Abstract:
:The effect of the novel, naturally occurring nucleotide cyclic diguanylic acid (c-di-GMP) on the lymphoblastoid CD4+ Jurkat cell line was studied. When exposed to 50 microM c-di-GMP, Jurkat cells exhibited a markedly elevated expression of the CD4 receptor of up to 6.3-fold over controls. C-di-GMP also causes blockage of the cell cycle at the S-phase, characterized by increased cellular thymidine uptake, reduction in G2/M-phase cells, increase in S-phase cells and decreased cell division. Additionally c-di-GMP naturally enters these cells and binds irreversibly to the P21ras protein. The effects described appear to be unique for c-di-GMP.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Steinberger O,Lapidot Z,Ben-Ishai Z,Amikam Ddoi
10.1016/s0014-5793(99)00036-8keywords:
subject
Has Abstractpub_date
1999-02-05 00:00:00pages
125-9issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(99)00036-8journal_volume
444pub_type
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