Recognition of an antiparallel beta-sheet structure of human epidermal growth factor by its receptor. Site-directed mutagenesis studies of Ala-30 and Asn-32.

abstract：:An improved method for expressing and purifying bovine pancreatic ribonuclease from a synthetic gene using the lambda promoter controlled by a temperature-sensitive repressor is described. The procedure involves isolation in the presence of a refolding buffer containing oxidized and reduced glutathione, under conditio...

journal_title：FEBS letters

authors： McGeehan GM,Benner SA

更新日期：1989-04-10 00:00:00

abstract：:The hypothesis that calpain subunits dissociate in the presence of Ca2+ has been tested by methods which avoid interference by Ca2+-induced aggregation and large subunit autolysis. Inactive Cys105Ser-m-calpain, bound either to Ni-NTA-agarose or to immobilized casein, after incubation with Ca2+, could be recovered in h...

journal_title：FEBS letters

authors： Dutt P,Arthur JS,Croall DE,Elce JS

更新日期：1998-10-09 00:00:00

abstract：:Cleavage of the envelope glycoprotein precursor gp160 of HIV-1 is a prerequisite for the infectivity of HIV-1, and occurs at least in part before gp160 reaches the cell surface. Kexin/subtilisin-related endopeptidases are proposed enzyme candidates for this intracellular processing. In this study, we reveal the possib...

journal_title：FEBS letters

authors： Okumura Y,Yano M,Murakami M,Mori S,Towatari T,Kido H

更新日期：1999-01-08 00:00:00

abstract：:Several catalytic properties of the hydrogenase from Scenedesmus obliquus have been examined to optimize the purification conditions. The Km-value for H2-evolution in the presence of the most effective electron mediator methylviologen is 0.66 mM. The pH-optimum is 6.3, the temperature-optimum is 50 degrees C and the e...

journal_title：FEBS letters

authors： Schnackenberg J,Schulz R,Senger H

更新日期：1993-07-19 00:00:00

abstract：:We review the evidence for H+ translocation by reconstituted cytochrome c oxidase; attention is paid to those aspects which we feel most open to criticism. Possible alternative hypotheses are assessed, with regard to experiments carried out to test them directly and with regard to published data. We conclude that, whi...

journal_title：FEBS letters

authors： Casey RP,Azzi A

更新日期：1983-04-18 00:00:00

abstract：:A mouse somatostatin (SS) receptor cDNA was cloned from neuroblastoma x glioma (NG108-15) cells. The sequence is almost identical to that of the mouse SSTR2 receptor [(1992) Proc. Natl. Acad. Sci. USA 89, 251)] but lacks about 300 nucleotides between transmembrane domain VII and the C-terminus. This spliced variant of...

journal_title：FEBS letters

authors： Vanetti M,Kouba M,Wang X,Vogt G,Höllt V

更新日期：1992-10-26 00:00:00

abstract：:A spectroscopic study of the flavocytochrome b enzyme, cellobiose oxidase, employing optical, NMR, EPR and near infra-red MCD techniques, has identified the axial ligands of the b-type haem. These are a histidine and a methionine, and this ligation set is discussed in relation to the functional role of the haem group....

journal_title：FEBS letters

authors： Cox MC,Rogers MS,Cheesman M,Jones GD,Thomson AJ,Wilson MT,Moore GR

更新日期：1992-07-28 00:00:00

abstract：:The phylogenetic distribution of divergently related protein families into the three domains of life (archaea, bacteria and eukaryotes) can signify the presence or absence of entire cellular processes in these domains and their ancestors. We can thus study the emergence of the major transitions during cellular evoluti...

journal_title：FEBS letters

authors： Ouzounis C,Kyrpides N

更新日期：1996-07-22 00:00:00

abstract：:An alpha beta heterodimer of the F1-ATPase of Rhodospirillum rubrum was isolated by extraction of chromatophores with LiCl. Each alpha beta heterodimer contains one tightly bound ADP, which is released upon removal of medium Mg2+. The dimer can be reversibly dissociated by removal of Mg(2+)-ions. The alpha beta hetero...

journal_title：FEBS letters

authors： Andralojc PJ,Harris DA

更新日期：1992-09-28 00:00:00

abstract：:The topic synthetic biology appears still as an 'empty basket to be filled'. However, there is already plenty of claims and visions, as well as convincing research strategies about the theme of synthetic biology. First of all, synthetic biology seems to be about the engineering of biology - about bottom-up and top-dow...

journal_title：FEBS letters

authors： Malinova V,Nallani M,Meier WP,Sinner EK

更新日期：2012-07-16 00:00:00

abstract：:In endocrine cells the regulated secretion of hormones, peptides, enzymes and neurotransmitters into the external medium occurs when mature secretory granules fuse with the plasma membrane. Secretory granules form at the trans-Golgi network (TGN) by envelopment of the dense-core aggregate of regulated secretory protei...

journal_title：FEBS letters

authors： Tooze SA

更新日期：1991-07-22 00:00:00

abstract：:To investigate the biochemical basis of the HIV-1 resistance to AZT we obtained the RT mutant containing four amino acid substitutions by an oligonucleotide-directed mutagenesis technique. Enzymatic properties of the wild type and mutant RTs were compared. 'AZT-resistant' mutations in RT were shown to be associated wi...

journal_title：FEBS letters

authors： Pokholok DK,Gudima SO,Yesipov DS,Dobrynin VN,Rechinsky VO,Kochetkov SN

更新日期：1993-07-05 00:00:00

abstract：:N-Oligosaccharyltransferase catalyzes the N-glycosylation of asparagine residues of nascent polypeptide chains in the endoplasmic reticulum, a pathway highly conserved in all eukaryotes. An enzymatically active complex was isolated from microsomal membranes from Saccharomyces cerevisiae, which is composed of four prot...

journal_title：FEBS letters

authors： Knauer R,Lehle L

更新日期：1994-05-09 00:00:00

abstract：:Azotobacter vinelandii (Av) and chroococcum (Ac) ferredoxin I contain [3Fe-4S]1 + 0 and [4Fe-4S]2+1+ clusters, when isolated aerobically, which undergo one-electron redox cycles at potentials of -460 +/- 10 mV (vs SHE) at pH 8.3 and -645 +/- 10 mV, respectively. The X-ray structure of Fd I (Av) reveals that the N-term...

journal_title：FEBS letters

authors： Thomson AJ

更新日期：1991-07-22 00:00:00

abstract：:A new GTP-binding protein serving as the specific substrate of islet-activating protein (IAP), pertussis toxin, was purified from porcine brain membranes as an alpha beta gamma-heterotrimeric structure. The alpha-subunit of the purified protein (alpha 40 beta gamma) had a molecular mass of 40 kDa and differed from tha...

journal_title：FEBS letters

authors： Katada T,Oinuma M,Kusakabe K,Ui M

更新日期：1987-03-23 00:00:00

abstract：:Replacing the essential Cys-149 by a selenocysteine into the active site of phosphorylating glyceraldehyde 3-phosphate dehydrogenase (GAPDH) from Bacillus stearothermophilus leads to a selenoGAPDH that mimics a selenoperoxidase activity. Saturation kinetics were observed with cumenyl and tert-butyl hydroperoxides, wit...

journal_title：FEBS letters

authors： Boschi-Muller S,Muller S,Van Dorsselaer A,Böck A,Branlant G

更新日期：1998-11-20 00:00:00

abstract：:Beta-thymosins sequester G-actin and preserve a pool of monomers of actin which constitute an important prerequisite for cellular function of the microfilament system. To study the influence of paraquat binding to G-actin on the interaction of G-actin with thymosin beta4 we determined the apparent dissociation constan...

journal_title：FEBS letters

authors： Huff T,Cappelletti G,Hannappel E

更新日期：1998-04-03 00:00:00

abstract：:Rhodamine 6G (3 microM) effectively inhibited delta pH-driven ATP synthesis in Methanobacterium thermoautotrophicum while delta pNa-driven ATP synthesis was not affected by it. Rhodamine 6G inhibited Mg(2+)-stimulated ATPase activity of membrane vesicles prepared from these cells but the ATPase catalytic sector detach...

journal_title：FEBS letters

authors： Smigán P,Majerník A,Greksák M

更新日期：1994-06-27 00:00:00

abstract：:Treatment of human vascular smooth muscle cells (SMC) with human alpha-thrombin greatly increased DNA synthesis and cell proliferation. Both the integrity of the catalytic site and that of the anion binding exosite were required for expression of this activity. Experiments employing Northerns indicated induction of c-...

journal_title：FEBS letters

authors： Kanthou C,Parry G,Wijelath E,Kakkar VV,Demoliou-Mason C

更新日期：1992-12-14 00:00:00

abstract：:The inhibitory glycine receptor (GlyR) is a ligand-gated chloride channel protein which displays developmental heterogeneity in the mammalian central nervous system. Here we describe 2 novel cDNA variants of the rat GlyR alpha 2 subunit and demonstrate that alternative splicing generates these 2 isoforms. The deduced ...

journal_title：FEBS letters

authors： Kuhse J,Kuryatov A,Maulet Y,Malosio ML,Schmieden V,Betz H

更新日期：1991-05-20 00:00:00

abstract：:Interleukin-8 (neutrophil-activating factor; NAP-1) has been crystallized by the vapour diffusion technique to give single crystals suitable for three-dimensional structural study at a resolution higher than 2.4 A. The crystals belong to the space group P3(1)21 or P3(2)21 and have unit cell dimensions a = b = 40.9 A, ...

journal_title：FEBS letters

authors： Auer M,Kallen J,Schleischitz S,Walkinshaw MD,Wasserbauer E,Ehn G,Lindley IJ

更新日期：1990-06-04 00:00:00

abstract：:The sequence of the 18 S rRNA of Tenebrio molitor is reported. A detailed secondary structure model for eukaryotic small subunit rRNAs is proposed. The model comprises 48 universal helices that eukaryotic and prokaryotic small subunit rRNAs have in common, plus a number of helices in areas of variable secondary struct...

journal_title：FEBS letters

authors： Hendriks L,De Baere R,Van Broeckhoven C,De Wachter R

更新日期：1988-05-09 00:00:00

abstract：:Translocation of folded proteins by the Tat system of Escherichia coli is believed to rely on the presence of phosphatidylethanolamine (PE) and the negatively charged phospholipids cardiolipin (CL) and phosphatidylglycerol (PG). Here, we show that while PE is indeed essential for activity, the Tat system is fully func...

journal_title：FEBS letters

authors： Rathmann C,Schlösser AS,Schiller J,Bogdanov M,Brüser T

更新日期：2017-09-01 00:00:00

abstract：:The Candida utilis QCR7 gene encoding subunit VII of the ubiquinol-cytochrome c oxidoreductase was isolated by functional complementation of the Saccharomyces cerevisiae subunit VII-null mutant. Several other subunit VII homologues as well as homologues for subunit VIII were identified by screening the GenBank databas...

journal_title：FEBS letters

authors： Boumans H,Berden JA,Grivell LA

更新日期：1995-07-10 00:00:00

abstract：:Histological studies suggest that adrenal medulla chromaffin cells in situ are polarised, but functional evidence is lacking. We present here the first demonstration for polarisation of exocytosis in isolated, spherical, bovine chromaffin cells. Cells were stimulated with 70 mM K(+) to cause a marked enhancement of ca...

journal_title：FEBS letters

authors： Cuchillo-Ibáñez I,Michelena P,Albillos A,García AG

更新日期：1999-10-01 00:00:00

abstract：:Phosphorylcholine, phosphorylcreatine, lactate, glutathione, glycine and leucine were identified in living HeLa cells in the first study of this cell type by 1H spin echo NMR spectroscopy. The technique has the advantage that it is non-invasive, providing detailed structural information on individual species present i...

journal_title：FEBS letters

authors： Reglinski J,Smith WE,Suckling CJ,Al-Kabban M,Watson ID,Stewart MJ

更新日期：1987-04-20 00:00:00

abstract：:A novel antimicrobial peptide, named misgurin, was isolated and characterized from the loach (mudfish), Misgurnus anguillicaudatus. The 21-amino-acid peptide with a molecular mass of 2502 Da was purified to homogeneity using a heparin-affinity column and C18 reverse-phase and gel-permeation high-performance liquid chr...

journal_title：FEBS letters

authors： Park CB,Lee JH,Park IY,Kim MS,Kim SC

更新日期：1997-07-14 00:00:00

abstract：:Using a cationic lipid bilayer, we show that DNA can be reliably adsorbed to the bilayer surface for atomic force microscopy (AFM) in aqueous buffers at high resolution. The measured width of the dsDNA is close to 2 nm, and a periodic modulation on dsDNA is reproducibly detected by the AFM. The measured period is 3.4 ...

journal_title：FEBS letters

authors： Mou J,Czajkowsky DM,Zhang Y,Shao Z

更新日期：1995-09-11 00:00:00

abstract：:(-)-Epigallocatechin-3-O-gallate (EGCG) exhibits anti-tumor activity mediated via the 67-kDa laminin receptor (67LR). In this study, we found that 67LR protein levels are reduced by exposure to low O(2) levels (5%), without affecting the expression of HIF-1α. We also found that EGCG-induced anti-cancer activity is abr...

journal_title：FEBS letters

authors： Tsukamoto S,Yamashita S,Kim YH,Kumazoe M,Huang Y,Yamada K,Tachibana H

更新日期：2012-09-21 00:00:00

abstract：:The maximal activities of the key glycolytic enzymes hexokinase and 6-phosphofructokinase, were reduced in brown adipose tissue in db/db mice compared to their lean littermates. Treatment of db/db mice with the thermogenic beta-adrenoceptor agonist, BRL 26830, restored normoglycaemia. The only significant increase in ...

journal_title：FEBS letters

authors： Young P,Cawthorne MA,Levy AL,Wilson K

更新日期：1984-10-15 00:00:00