Amino acids 225-235** of the protein C serine-protease domain are important for the interaction with the thrombin-thrombomodulin complex.

abstract：:Chaperone-mediated autophagy (CMA) is a lysosomal pathway that participates in the degradation of cytosolic proteins. CMA is activated by starvation and in response to stressors that result in protein damage. The selectivity intrinsic to CMA allows for removal of damaged proteins without disturbing nearby functional o...

journal_title：FEBS letters

authors： Kon M,Cuervo AM

更新日期：2010-04-02 00:00:00

abstract：:The Ca(2+)-activated photoprotein, aequorin, contains six tryptophan residues and has a bioluminescence emission maximum at 465 nm. On converting the six tryptophan residues to phenylalanine, the mutant aequorins exhibited varied luminescence activities and spectra, but one mutant, with tryptophan-86 replaced by pheny...

journal_title：FEBS letters

authors： Ohmiya Y,Ohashi M,Tsuji FI

更新日期：1992-04-20 00:00:00

abstract：:We recently purified two closely related 33 kDa proteins from rat hepatic cytosol, designated bile acid binder I and II, which selectively bind bile acids with comparable affinity as glutathione S-transferase B. This work has now been extended to human liver in which we have identified a similar cytosolic binding acti...

journal_title：FEBS letters

authors： Stolz A,Sugiyama Y,Kuhlenkamp J,Kaplowitz N

更新日期：1984-11-05 00:00:00

abstract：:The oxysterol binding proteins (OSBPs) are believed to control cholesterol homeostasis but their precise mechanism of action is not well understood. The Dictyostelium osbA gene encodes a predicted OSBP, OSBPa, which lacks the PH domain that in most other OSBPs directs targetting to the Golgi. OSBPa instead localises s...

journal_title：FEBS letters

authors： Fukuzawa M,Williams JG

更新日期：2002-09-11 00:00:00

abstract：:The influence of aniso-osmolarity on the activity of the MAP kinases Erk-1 and Erk-2 was studied in C6 glioma cells. Hypo-osmotic treatment (205 mosmol/l) led to an increased activity of Erk-1 and Erk-2 within 3 min, which became maximal at 10 min and returned to basal level within 120 min. In contrast, Erk activity w...

journal_title：FEBS letters

authors： Sinning R,Schliess F,Kubitz R,Häussinger D

更新日期：1997-01-03 00:00:00

abstract：:There is considerable epidemiological evidence that tea drinking lowers the risk of heart disease. However, the mechanism by which tea can be protective is unknown. Hamsters were fed a normal or high cholesterol diet for 2 weeks and drank green or black tea ad libitum. The plasma lipid profile was significantly improv...

journal_title：FEBS letters

authors： Vinson JA,Dabbagh YA

更新日期：1998-08-14 00:00:00

abstract：:Normal transcription and postranslational processing of the preprotachykinin (PPT)-I gene and regulated release of substance P and neurokinin A by the rat pancreatic endocrine cell-line, RINm5F, has been demonstrated, using radioimmunoassays (RIAs), reversed-phase (rp)HPLC and Northern blot analysis. This is the first...

journal_title：FEBS letters

authors： McGregor GP,Hartel R,Fehmann HC,Lankat-Buttgereit B,Göke B,Göke R,Voigt K

更新日期：1992-11-09 00:00:00

abstract：:Treatment of human amniotic cells (UAC) with human interferon-alpha (Hu-IFN alpha) or phorbol myristate acetate (PMA) resulted in translocation of protein kinase C (PK-C) activity from the cytosol fraction to that of the membranes. Analysis of 32P incorporation into phospholipid fractions and studies of alterations in...

journal_title：FEBS letters

authors： Premecz G,Markovits A,Bagi G,Farkas T,Földes I

更新日期：1989-06-05 00:00:00

abstract：:The R2 protein component of mouse ribonucleotide reductase has been obtained from overproducing Escherichia coli bacteria. It has been crystallized using NaCl as precipitant. The crystals are orthorhombic, space group C222(1), with cell dimensions a = 76.9 A, b = 108.9 A, c = 92.7 A and diffract to at least 2.5 A. The...

journal_title：FEBS letters

authors： Nielsen BB,Kauppi B,Thelander M,Thelander L,Larsen IK,Eklund H

更新日期：1995-10-16 00:00:00

abstract：:Hypermethylated in cancer, a new candidate tumor suppressor gene located in 17p13.3, encodes a protein with five Krüppel-like C2H2 zinc finger motifs and a N-terminal protein/protein interaction domain called broad complex, tramtrack and bric à brac/poxviruses and zinc finger domain. Hypermethylated in cancer appears ...

journal_title：FEBS letters

authors： Guerardel C,Deltour S,Leprince D

更新日期：1999-05-28 00:00:00

abstract：:The role of the Ca(2+)-activated tyrosine kinase, Pyk2, in the pleiotropic coupling of nerve cell stimulation to the MAP kinase cascade still remains undefined. Using a panel of PC12 clones, one of which was defective in Pyk2, we demonstrate (1) that the MAP kinase response induced by a [Ca(2+)](i) rise (following app...

journal_title：FEBS letters

authors： Barsacchi R,Heider H,Girault J,Meldolesi J

更新日期：1999-11-19 00:00:00

abstract：:On the basis of recent results, we propose a model for DNA polymerase translocation along DNA. Human immunodeficiency virus reverse transcriptase is taken as an example. According to the model, movement of the enzyme is the result of transition of the enzyme-bound DNA from the A- to B-form which is accompanied by leng...

journal_title：FEBS letters

authors： Wlassoff WA,Dymshits GM,Lavrik OI

更新日期：1996-07-15 00:00:00

abstract：:Treatment of Chinese hamster ovary K1 cells with phosphatidylserine (PS) caused typical apoptosis with distinct morphological and biochemical features in a dose- and time-dependent manner. However, unlike camptothecin-induced apoptosis, changes in mitochondrial transmembrane potential were not observed. In addition, c...

journal_title：FEBS letters

authors： Miyato Y,Ibuki Y,Ohyama H,Yamada T,Goto R

更新日期：2001-08-24 00:00:00

abstract：:Human red cells suspended in lead-citrate buffers (2.6 microM Pb2+) take up much less Pb than predicted from studies of equilibrium binding of Pb to haemolysates. Pb uptake is increased by ATP depletion, or by loading at 0 degrees C. Tracer studies with 203Pb indicate that the low uptake at 37 degrees C in the presenc...

journal_title：FEBS letters

authors： Simons TJ

更新日期：1984-07-09 00:00:00

abstract：:Lysine-rich phosphorylated cystatin alpha (P-cystatin alpha) from newborn rat epidermis is a good substrate for epidermal transglutaminase (TGase) and also one of the component proteins of cornified envelope in the stratum corneum. Since the filaggrin linker segment peptide was efficiently conjugated with P-cystatin a...

journal_title：FEBS letters

authors： Takahashi M,Tezuka T,Kakegawa H,Katunuma N

更新日期：1994-03-07 00:00:00

abstract：:The CLN3 gene encodes an integral membrane protein of unknown function. Mutations in CLN3 can cause juvenile neuronal ceroid lipofuscinosis, or Batten disease, an inherited neurodegenerative lysosomal storage disease affecting children. Here, we report a topological study of the CLN3 protein using bioinformatic approa...

journal_title：FEBS letters

authors： Nugent T,Mole SE,Jones DT

更新日期：2008-04-02 00:00:00

abstract：:500 MHz 1H NMR studies of human epidermal growth factor are described. The backbone resonances of the 1-48 derivative of hEGF have been assigned using two-dimensional techniques. Analysis of the type and magnitude of the observed sequential nuclear Overhauser effects and the NH-alpha CH spin-spin coupling constants al...

journal_title：FEBS letters

authors： Carver JA,Cooke RM,Esposito G,Campbell ID,Gregory H,Sheard B

更新日期：1986-09-01 00:00:00

abstract：:Two hybrid proteins between Pseudomonas aeruginosa exotoxin A (PE) and poliovirus protease 2Apro have been generated. One hybrid protein contains the poliovirus 2Apro sequence replacing the region of PE corresponding to amino acids 413-607. The other hybrid contains in addition the transforming growth factor sequence....

journal_title：FEBS letters

authors： Novoa I,Feduchi E,Carrasco L

更新日期：1994-11-21 00:00:00

abstract：:We have recently developed a method to produce native human proinsulin using a bacterial expression system. A proinsulin fusion protein was recovered from inclusion bodies and cleaved using cyanogen bromide. The released proinsulin polypeptide was S-sulfonated and purified by anion exchange chromatography. Following r...

journal_title：FEBS letters

authors： Cowley DJ,Mackin RB

更新日期：1997-02-03 00:00:00

abstract：:The orientation of the transition dipole moments in the ground state and the M-intermediate of bacteriorhodopsin were determined by time-resolved and steady-state polarized absorption spectroscopy on samples of oriented immobilized purple membranes. The angle between the transition dipole moment and the membrane norma...

journal_title：FEBS letters

authors： Otto H,Heyn MP

更新日期：1991-11-18 00:00:00

abstract：:Nitric oxide, as well as being a major regulator of vascular reactivity, has been shown to be one of the mediators of cytotoxicity in macrophages. This cytotoxic effect seems to be due to the interaction between nitric oxide and oxygen-related free radicals. This study shows that, in vitro, nitric oxide reacts with hy...

journal_title：FEBS letters

authors： Noronha-Dutra AA,Epperlein MM,Woolf N

更新日期：1993-04-19 00:00:00

abstract：:In thrombin-stimulated platelets alpha IIb beta 3 integrin engagement triggers both phosphatidylinositol 3',4'-bisphosphate synthesis and calpain activation. We checked the possible involvement of calpains in phosphatidylinositol 3-kinase signalling pathway using a cell permeant specific inhibitor of calpains, calpept...

journal_title：FEBS letters

authors： Montsarrat N,Racaud-Sultan C,Mauco G,Plantavid M,Payrastre B,Breton-Douillon M,Chap H

更新日期：1997-03-03 00:00:00

abstract：:Male germ cell Rac GTPase-activating protein (MgcRacGAP) is a core regulator of cytokinesis. Furthermore, it appears to be involved in human oncogenesis through cytokinesis-independent mechanisms and has been reported to be essential for nuclear translocation of signal transducer and activator of transcription (STAT) ...

journal_title：FEBS letters

authors： van Adrichem AJ,Wennerberg K

更新日期：2015-12-21 00:00:00

abstract：:Cytosine methylation of binding sites for transcription factors is a straightforward mechanism to prevent transcription, while data on an indirect mechanism, by methylation outside of the factor binding sites, are still scarce. We have studied the latter effect using a model promoter construct. For this, a 69 bp G + C...

journal_title：FEBS letters

authors： Hug M,Silke J,Georgiev O,Rusconi S,Schaffner W,Matsuo K

更新日期：1996-02-05 00:00:00

abstract：:Signal recognition particle (SRP) interacts with the signal sequence in nascent secretory and membrane proteins and directs them to the membrane of the endoplasmic reticulum. Membrane targeting is mediated by the 68 and the 72 kDa proteins of SRP. We have cloned and sequenced cDNA encoding the 68 kDa protein of canine...

journal_title：FEBS letters

authors： Herz J,Flint N,Stanley K,Frank R,Dobberstein B

更新日期：1990-12-10 00:00:00

abstract：:Myeloperoxidase (MPO) is the most abundant protein in neutrophils and plays a central role in microbial killing and inflammatory tissue damage. Because most of the non-steroidal anti-inflammatory drugs and other drugs contain a thiol group, it is necessary to understand how these substrates are oxidized by MPO. We hav...

journal_title：FEBS letters

authors： Burner U,Jantschko W,Obinger C

更新日期：1999-01-29 00:00:00

abstract：:The cellular uptake of a model antisense oligonucleotide complementary to 21 bases of the bovine GLUT-1 glucose transporter mRNA and a model vasopressin peptide that were biotinylated, was markedly stimulated by the presence of avidin, a cationic protein. Conversely, the bacteria homologue of avidin, streptavidin, whi...

journal_title：FEBS letters

authors： Pardridge WM,Boado RJ

更新日期：1991-08-19 00:00:00

abstract：:Cellulysin, a crude cellulase from the plant parasitic fungus Trichoderma viride, induces the biosynthesis of volatiles in higher plants (Nicotiana plumbaginifolia, Phaseolus lunatus, and Zea mays) when applied to cut petioles by the transpiration stream. The pattern of the emitted volatiles largely resembles that fro...

journal_title：FEBS letters

authors： Piel J,Atzorn R,Gäbler R,Kühnemann F,Boland W

更新日期：1997-10-20 00:00:00

abstract：:Two synthetic photoactive azidoarylphosphatidylcholines were used to investigate the level of interaction between D-beta-hydroxybutyrate apodehydrogenase (apoBDH), an amphipathic membrane protein, with the hydrophobic domain of phospholipids. The two synthetic lecithins, PL I (1-myristoyl-2-12-N-(4-azido-2-nitrophenyl...

journal_title：FEBS letters

authors： el Kebbaj MS,Berrez JM,Lakhlifi T,Morpain C,Latruffe N

更新日期：1985-03-11 00:00:00

abstract：:In higher eukaryotes, nine aminoacyl-tRNA synthetases form a multienzyme complex also comprising the three auxiliary proteins p18, p38 and p43, of apparent molecular masses of 18, 38 and 43 kDa. The function of these proteins, invariably found associated to the synthetase components of the complex, is unknown. In orde...

journal_title：FEBS letters

authors： Quevillon S,Mirande M

更新日期：1996-10-14 00:00:00