Abstract:
:The hyperthermophilic archaeon Sulfolobus solfataricus metabolises glucose and galactose by a 'promiscuous' non-phosphorylative variant of the Entner-Doudoroff pathway, in which a series of enzymes have sufficient substrate promiscuity to permit the metabolism of both sugars. Recently, it has been proposed that the part-phosphorylative Entner-Doudoroff pathway occurs in parallel in S. solfataricus as an alternative route for glucose metabolism. In this report we demonstrate, by in vitro kinetic studies of D-2-keto-3-deoxygluconate (KDG) kinase and KDG aldolase, that the part-phosphorylative pathway in S. solfataricus is also promiscuous for the metabolism of both glucose and galactose.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Lamble HJ,Theodossis A,Milburn CC,Taylor GL,Bull SD,Hough DW,Danson MJdoi
10.1016/j.febslet.2005.11.028keywords:
subject
Has Abstractpub_date
2005-12-19 00:00:00pages
6865-9issue
30eissn
0014-5793issn
1873-3468pii
S0014-5793(05)01391-8journal_volume
579pub_type
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