Abstract:
:The non-selective gastrin/cholecystokinin receptor antagonists proglumide and benzotript inhibit colon carcinoma cell proliferation by binding to the 78 kDa gastrin-binding protein (GBP) (Baldwin, Proc. Natl. Acad. Sci. USA, 91 (1994) 7593-7597). However, although most colon carcinoma cell lines synthesize progastrin, production of mature amidated gastrin17 has not been observed. In order to define the structural requirements for the binding of gastrin to the GBP the affinities of various fragments of amidated and C-terminally extended gastrin17 for the GBP have been measured. The results indicate that the GBP recognizes both N- and C-termini of gastrin17. Moreover since C-terminal amidation is not a prerequisite for binding of gastrin to the GBP, the GBP is a potential target for the autocrine effects of progastrin.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Baldwin GSdoi
10.1016/0014-5793(95)00017-4subject
Has Abstractpub_date
1995-02-06 00:00:00pages
97-100issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(95)00017-4journal_volume
359pub_type
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