The kinetics of the interaction between the actin-binding domain of alpha-actinin and F-actin.

abstract：:A pronounced transitory increase of histone acetyltransferase (EC 2.3.1.48) activity before the onset of DNA replication is shown to occur in regenerating rat liver. This effect is followed by an increase in the H1/H1(0) ratio at the start of DNA synthesis; H1(0) de is replaced by H1. Histone acetylation and H1/H1(0) ...

journal_title：FEBS letters

authors： Weiss G,Puschendorf B

更新日期：1988-09-26 00:00:00

abstract：:Platelet aggregation is accompanied by the tyrosine phosphorylation of several proteins including syk. However, some of these proteins are not identified. Recent studies showed that LAT is a syk substrate and is tyrosine phosphorylated during T cell stimulation. In this study, we demonstrated that LAT is present in pl...

journal_title：FEBS letters

authors： Sarkar S

更新日期：1998-12-28 00:00:00

abstract：:The fluorescent calcium indicator 'quin2' was used to demonstrate changes in cytoplasmic calcium concentrations in bovine anterior pituitary cells. The basal calcium concentration was 0.21 +/- 0.02 microM (mean of 4 cell preparations). Thyroliberin (TRH) (10(-10) - 10(-6) M) rapidly and at the higher concentrations tr...

journal_title：FEBS letters

authors： Schofield JG

更新日期：1983-08-08 00:00:00

abstract：:Photosystem I (PSI) complex of Anabaena variabilis ATCC 29413 consists of at least 11 subunits, 9 of which are resolved by high resolution gel electrophoresis. N-terminal amino acid sequences of the four subunits with molecular masses of 6.8, 5.2, 4.8 and 3.5 kDa were determined. Based on the sequence homology, the 3....

journal_title：FEBS letters

authors： Ikeuchi M,Nyhus KJ,Inoue Y,Pakrasi HB

更新日期：1991-08-05 00:00:00

abstract：:Electron microscopy of mammalian smooth muscle myosin rods showed them to be 153 +/- 7 nm (SD) long, and to bend sharply (greater than 90 degrees) but infrequently, and pH independently (range 6.5-9.5), at a single site 45 +/- 4 nm from one end of the molecule. Light meromyosin (LMM) preparations were 99 +/- 10 nm lon...

journal_title：FEBS letters

authors： Cross RA

更新日期：1984-10-15 00:00:00

abstract：:The mechanism of store-operated Ca2+ inflow in hepatocytes was investigated using fluo-3 and fura-2 to monitor changes in the concentration of intracellular free Ca2+ in single cells, and 1-(alpha-glycerophosphoryl)-myo-inositol 4,5-diphosphate, P4(5)-1-(2-nitrophenyl)ethyl ester ('caged' GPIP2) and 'caged' guanosine ...

journal_title：FEBS letters

authors： Berven LA,Barritt GJ

更新日期：1994-06-13 00:00:00

abstract：:Based on the amino acid alignment, Caenorhabditis elegans F32D1.1 was identified to be a homologue of the mammalian fidgetin. We produced and purified the F32D1.1 protein by using a baculovirus-expression system. F32D1.1 has an ATPase activity, which is sensitive to N-ethylmaleimide. Km and Vmax for the ATPase activit...

journal_title：FEBS letters

authors： Yakushiji Y,Yamanaka K,Ogura T

更新日期：2004-12-03 00:00:00

abstract：:The tendency of DNA to form fibers upon condensation with counterions is reviewed. It is shown that chromatin fibers may acquire a relatively constant diameter of about 30 nm simply as an optimal size achieved upon neutralization of DNA, without requiring a repetitive internal structure. Thus the size of chromatin fib...

journal_title：FEBS letters

authors： Subirana JA

更新日期：1992-05-11 00:00:00

abstract：:Compared with algal and cyanobacterial cytochrome c(6), cytochrome c(6A) from higher plants contains an additional loop of 12 amino acid residues. We have determined the first crystal structure of cytochrome c(6A) from Arabidopsis thaliana at 1.5 Angstrom resolution in order to help elucidate its function. The overall...

journal_title：FEBS letters

authors： Chida H,Yokoyama T,Kawai F,Nakazawa A,Akazaki H,Takayama Y,Hirano T,Suruga K,Satoh T,Yamada S,Kawachi R,Unzai S,Nishio T,Park SY,Oku T

更新日期：2006-06-26 00:00:00

abstract：:A macrophage migration inhibitory factor (MIF), originally described as a product of activated lymphocytes, has been defined as a 12 kDa protein, expressed in a wide variety of tissues. Here MIF is identified as a phenylpyruvate tautomerase (EC 5.3.2.1) having p-hydroxyphenylpyruvate and phenylpyruvate as its natural ...

journal_title：FEBS letters

authors： Rosengren E,Aman P,Thelin S,Hansson C,Ahlfors S,Björk P,Jacobsson L,Rorsman H

更新日期：1997-11-03 00:00:00

abstract：:Apoptotic proteases cleave and inactivate survival signaling molecules such as Akt/PKB, phospholipase C (PLC)-gamma1, and Bcl-2. We have found that treatment of A431 cells with tumor necrosis factor-alpha in the presence of cycloheximide resulted in the cleavage of epidermal growth factor receptor (EGFR) as well as th...

journal_title：FEBS letters

authors： Bae SS,Choi JH,Oh YS,Perry DK,Ryu SH,Suh PG

更新日期：2001-02-23 00:00:00

abstract：:Data are presented on the formation of a linoleic acid oxygenation product under basal conditions by guinea pig alveolar macrophages (AM). Under non-stimulated conditions 20 X 10(6) AM released about 1 nmol 9-hydroxylinoleic acid (9-HODE) during a 15 min incubation. Furthermore, AM released more than 2 nmol 12-hydroxy...

journal_title：FEBS letters

authors： Engels F,Willems H,Nijkamp FP

更新日期：1986-12-15 00:00:00

abstract：:In thrombin-stimulated platelets alpha IIb beta 3 integrin engagement triggers both phosphatidylinositol 3',4'-bisphosphate synthesis and calpain activation. We checked the possible involvement of calpains in phosphatidylinositol 3-kinase signalling pathway using a cell permeant specific inhibitor of calpains, calpept...

journal_title：FEBS letters

authors： Montsarrat N,Racaud-Sultan C,Mauco G,Plantavid M,Payrastre B,Breton-Douillon M,Chap H

更新日期：1997-03-03 00:00:00

abstract：:The effects of guanine nucleotides on arachidonic acid (AA) release were studied in intact and saponin-permeabilized human platelets. While GTP[S] itself caused a stimulation of AA release in permeabilized cells, GTP[S], GDP[S], GTP, ATP and other nucleotides inhibited AA release in response to thrombin and other agon...

journal_title：FEBS letters

authors： Rehm AG,Wu H,Halenda SP

更新日期：1988-07-18 00:00:00

abstract：:Primary cultures of rat dorsal root ganglionic (DRG) cells were stained with isoform-specific antibodies against non-muscle myosin II. Antibodies against the brain type myosin (MIIB) stained the peripheries of growth cones and non-neuronal cells. Double staining of the cells with the anti-myosin antibodies and rhodami...

journal_title：FEBS letters

authors： Cheng TP,Murakami N,Elzinga M

更新日期：1992-10-19 00:00:00

abstract：:In this work we report that the Saccharomyces cerevisiae RAD9, RAD24, RAD17, MEC1, MEC3 and RAD53 checkpoint genes are required for efficient non-homologous end joining (NHEJ). RAD9 and RAD24 function additionally in this process. Defective NHEJ in rad9Delta-rad24Delta, but not yku80Delta cells, is only partially resc...

journal_title：FEBS letters

authors： de la Torre-Ruiz M,Lowndes NF

更新日期：2000-02-11 00:00:00

abstract：:In lower and higher eukaryotes, a family of tightly related proteins designated eRF1 (for eukaryotic release factor 1) catalyses termination of protein synthesis at all three stop codons. The human genome contains four eRF1 homologous sequences localised on chromosomes 5, 6, 7 and X. We report here the cloning and the...

journal_title：FEBS letters

authors： Guenet L,Toutain B,Guilleret I,Chauvel B,Deaven LL,Longmire JL,Le Gall JY,David V,Le Treut A

更新日期：1999-07-02 00:00:00

abstract：:Iron regulatory protein 2 (IRP2) is a critical switch for cellular and systemic iron homeostasis. In iron-deficient or hypoxic cells, IRP2 binds to mRNAs containing iron responsive elements (IREs) and regulates their expression. Iron promotes proteasomal degradation of IRP2 via the F-box protein FBXL5. Here, we explor...

journal_title：FEBS letters

authors： Hausmann A,Lee J,Pantopoulos K

更新日期：2011-02-18 00:00:00

abstract：:We have reported that histone deacetylase (HDAC) inhibitors activate a member of the INK4 family, the p19INK4d gene, causing G1 phase arrest. We report here that HDAC inhibitor, Trichostatin A, activates another member of the INK4 family, the p18INK4c gene, through its promoter in Jurkat cells. Interestingly, the acti...

journal_title：FEBS letters

authors： Yokota T,Matsuzaki Y,Sakai T

更新日期：2004-09-10 00:00:00

abstract：:As the initiator of DNA double-strand breaks during meiosis in Saccharomyces cerevisiae, the SPO11 protein is essential for recombination. Similarity between SPO11 and archaebacterial TOP6A proteins points to evolutionary specialization of a DNA cleavage function for meiotic recombination. To determine whether this ex...

journal_title：FEBS letters

authors： Shannon M,Richardson L,Christian A,Handel MA,Thelen MP

更新日期：1999-12-03 00:00:00

abstract：:o-Aminophenol was found to be rapidly metabolized to a brown compound in the presence of purified human oxy- and methemoglobin, coupled with the oxidation and reduction of these hemoglobins by o-aminophenol. The final product of o-aminophenol was identified as 2-aminophenoxazine-3-one, by using spectrophotometry and H...

journal_title：FEBS letters

authors： Tomoda A,Yamaguchi J,Kojima H,Amemiya H,Yoneyama Y

更新日期：1986-02-03 00:00:00

abstract：:The inhibitory effect of actin on protein phosphorylation by three distinct protein kinases (CK-II, A-kinase and MAP-kinase) was examined in vitro. It was found that: (i) actin inhibits the activities of alpha-monomeric CK-II (CK-IIalpha) as well as oligomeric CK-II (alpha2beta2) in a dose-dependent manner, but has no...

journal_title：FEBS letters

authors： Karino A,Tanoue S,Fukuda M,Nakamura T,Ohtsuki K

更新日期：1996-12-02 00:00:00

abstract：:Soluble aluminium (Al(3+)) is the major constraint to plant growth on acid soils. Plants have evolved mechanisms to tolerate Al(3+) and one type of mechanism relies on the efflux of organic anions that protect roots by chelating the Al(3+). Al(3+) resistance genes of several species have now been isolated and found to...

journal_title：FEBS letters

authors： Delhaize E,Gruber BD,Ryan PR

更新日期：2007-05-25 00:00:00

abstract：:Decarboxylation of pyruvate by pyruvate decarboxylase (EC 4.1.1.1) was performed in a reaction mixture containing 50% deuterium. The isolated product, acetaldehyde, was investigated directly by 1H NMR and by mass spectrometry after conversion to the 2,4-dinitrophenyl hydrazone. The protium content of 56% at acetaldehy...

journal_title：FEBS letters

authors： Ermer J,Schellenberger A,Hübner G

更新日期：1992-03-09 00:00:00

abstract：:In a previous study [(1987) J. Mol. Biol. 194, 663-673], we isolated ten insertion/deletion mutants (indels) of the maltose binding protein for which the maltose binding constant was only a little or not at all affected. In this paper, we have localized these mutations in the recently solved three-dimensional structur...

journal_title：FEBS letters

authors： Betton JM,Martineau P,Saurin W,Hofnung M

更新日期：1993-06-28 00:00:00

abstract：:Mycobacterium tuberculosis contains multiple versions of the accA and accD genes that encode the alpha- and beta-subunits of at least three distinct multi-functional acyl-CoA carboxylase complexes. Because of its proposed involvement in pathogenic M. tuberculosis survival, the high-resolution crystal structure of the ...

journal_title：FEBS letters

authors： Holton SJ,King-Scott S,Nasser Eddine A,Kaufmann SH,Wilmanns M

更新日期：2006-12-22 00:00:00

abstract：:Ligand-directed functional selectivity has stimulated much interest in the molecular delineation of drug pharmacology and the process of drug discovery. Here we describe the development of a label-free optical biosensor with microfluidics for whole cell sensing. The microfluidics controls the duration of agonist expos...

journal_title：FEBS letters

authors： Goral V,Wu Q,Sun H,Fang Y

更新日期：2011-04-06 00:00:00

abstract：:The complete sequence of a 94 amino acid human seminal plasma polypeptide exhibiting inhibin-like activity is presented. This molecule, called beta-inhibin, selectively and specifically suppresses the release of pituitary FSH in vivo as well as in vitro. It does not affect the secretion of LH. Such a novel acidic prot...

journal_title：FEBS letters

authors： Seidah NG,Arbatti NJ,Rochemont J,Sheth AR,Chrétien M

更新日期：1984-10-01 00:00:00

abstract：:To determine the regulatory mechanism for human tyrosine hydroxylase, we examined modulations of the activity of the enzyme from human pheochromocytoma by cyclic AMP-dependent protein kinase, calmodulin-dependent protein kinase II and polyanion. The most remarkable activation was observed when the enzyme was assayed a...

journal_title：FEBS letters

authors： Okuno S,Kanayama Y,Fujisawa H

更新日期：1989-08-14 00:00:00

abstract：:Specific receptors for angiotensin II (AII) were expressed in albino Xenopus laevis oocytes co-injected with poly(A)+ mRNA isolated from rat adrenal cortex and the calcium-specific photoprotein, aequorin. In such oocytes, AII elicited rapid, dose-dependent rises in cytosolic free calcium with light emission responses ...

journal_title：FEBS letters

authors： Sandberg K,Markwick AJ,Trinh DP,Catt KJ

更新日期：1988-12-05 00:00:00