Abstract:
:In this work we report that the Saccharomyces cerevisiae RAD9, RAD24, RAD17, MEC1, MEC3 and RAD53 checkpoint genes are required for efficient non-homologous end joining (NHEJ). RAD9 and RAD24 function additionally in this process. Defective NHEJ in rad9Delta-rad24Delta, but not yku80Delta cells, is only partially rescued by imposing G1 or G2/M delays. Thus, checkpoint functions other than transient cell cycle delays may be required for normal levels of NHEJ. Epistasis analysis also indicated that YKU80 and RAD9/RAD24 function in the same pathway for repair of lesions caused by MMS and gamma-irradiation. Unlike NHEJ, the checkpoint pathway is not required for efficient site-specific integration of plasmid DNA into the yeast genome, which is RAD52-dependent, but RAD51-independent.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
de la Torre-Ruiz M,Lowndes NFdoi
10.1016/s0014-5793(00)01180-7keywords:
subject
Has Abstractpub_date
2000-02-11 00:00:00pages
311-5issue
2-3eissn
0014-5793issn
1873-3468pii
S0014579300011807journal_volume
467pub_type
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