Abstract:
:To demonstrate the interaction of calpastatin (CS) domain L (CSL) with Cav1.2 channel, we investigated the binding of CSL with various C-terminus-derived peptides at≈free, 100 nM, 10 μM, and 1mM Ca(2+) by using the GST pull-down assay method. Besides binding with the IQ motif, CSL was also found to bind with the PreIQ motif. With increasing [Ca(2+)], the affinity of the CSL-IQ interaction gradually decreased, and the affinity of the CSL-PreIQ binding gradually increased. The results suggest that CSL may bind with both the IQ and PreIQ motifs of the Cav1.2 channel in different Ca(2+)-dependent manners.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Sun W,Feng R,Hu H,Guo F,Gao Q,Shao D,Yin D,Wang H,Sun X,Zhao M,Minobe E,Sun Y,Jiao G,Kameyama M,Hao Ldoi
10.1016/j.febslet.2014.01.019subject
Has Abstractpub_date
2014-03-03 00:00:00pages
665-71issue
5eissn
0014-5793issn
1873-3468pii
S0014-5793(14)00046-5journal_volume
588pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::In order to study the transactivational property of the X gene in the methylotropic yeast Pichia pastoris, a Rous sarcoma virus-chloramphenicol acetyltransferase (RSV-CAT) cassette was co-transformed and integrated into the host yeast strain as a reporter which showed an overwhelming CAT activity. Immunoprecipitation ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00930-8
更新日期:1999-07-30 00:00:00
abstract::The 500 MHz 1H NMR spectrum of a 68-residue peptide, encompassing the rat thyroid transcription factor 1 homeodomain (TTF-1 HD), was fully assigned using standard 2D NMR methodology. The secondary structure elements and their spatial organization were determined and led to a structure very similar to that previously d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80845-l
更新日期:1993-12-28 00:00:00
abstract::In bacterial polypeptide synthesis aminoacyl-tRNA (aa-tRNA) bound to elongation factor Tu (EF-Tu) and GTP is part of a crucial intermediate ribonucleoprotein complex involved in the decoding of messenger RNA. The conformation and topology as well as the affinity of the macromolecules in this ternary aa-tRNA X EF-Tu X ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(87)80664-6
更新日期:1987-06-15 00:00:00
abstract::Ischemic preconditioning (IPrec) improves post-ischemic dysfunctions of the myocardium along with activation of protein kinase C isozymes including PKCdelta. Moreover, expression of cardio-protective determinants can reduce ischemic damages. Because IPrec is limited in aged hearts, we assessed in an experimental model...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01342-5
更新日期:2003-12-18 00:00:00
abstract::The archaeal exosome, a protein complex responsible for phosphorolytic degradation and tailing of RNA, has an RNA-binding platform containing Rrp4, Csl4, and DnaG. Aiming to detect novel interaction partners of the exosome, we copurified Nop5, which is a part of an rRNA methylating ribonucleoprotein complex, with the ...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12915
更新日期:2017-12-01 00:00:00
abstract::The eukaryotic transcription factor NF-kappaB is activated by a large variety of stimuli. We have recently shown that ER stress, caused by an aberrant accumulation of membrane proteins within this organelle, also activates NF-kappaB. Here, we show that activation of NF-kappaB by ER stress requires an increase in the i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00800-9
更新日期:1996-08-26 00:00:00
abstract::A number of human diseases can be linked to aberrations in protein folding which cause an imbalance in protein homeostasis. Molecular chaperones, including heat shock proteins, act to assist protein folding, stability and activity in the cell. Attention has begun to focus on modulating the expression and/or activity o...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2007.05.040
更新日期:2007-07-31 00:00:00
abstract::Two new three-disulfide intermediates have been found to be populated in the oxidative folding pathway of bovine pancreatic ribonuclease A at a low temperature (15 degrees C). These intermediates, des-[26-84] and des-[58-110], possess all but one of the four native disulfide bonds and have a stable tertiary structure,...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01391-5
更新日期:1999-11-05 00:00:00
abstract::The effects of metal cations on the activation of recombinant human SK4 (also known as hIK1 or hKCa4) channels, expressed in HEK 293 cells, were tested using patch clamp recording. Of the nine metals tested, cobalt, iron, magnesium, and zinc did not activate the SK4 channels when applied, at concentrations up to 100 m...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00194-5
更新日期:1999-03-05 00:00:00
abstract::In this study, we provide evidence that endoplasmic reticulum (ER) stress suppresses DNA double-strand break (DSB) repair and increases radiosensitivity of tumor cells by altering Rad51 levels. We show that the ER stress inducer tunicamycin stimulates selective degradation of Rad51 via the 26S proteasome, impairing DS...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.08.030
更新日期:2013-10-11 00:00:00
abstract::This study describes for the first time the amphiphilicity of the protein moiety of proteoglycogen. Glycogenin but not proteoglycogen associates to phospholipid vesicles and forms by itself stable Gibbs and Langmuir monolayers at the air-buffer interface. The adsorption free energy (-6.7 kcal/mol) and the glycogenin c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03192-1
更新日期:2001-12-07 00:00:00
abstract::LLC-PK1L cells, a kidney-derived cell line grown in defined medium, possess a vasopressin-sensitive adenylate cyclase. Somatostatin was able to inhibit the vasopressin-induced increase in adenylate cyclase activity, without affecting the basal enzyme activity. This inhibition was competitive. No effect of somatostatin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80304-x
更新日期:1984-04-24 00:00:00
abstract::The two activators of protein kinase C, oleoylacetylglycerol (OAG) and dioctanoylglycerol (DOG), are able to induce a concentration-dependent rise in cytoplasmic free Ca2+ concentration in gel-filtered human platelets, detected as an increase in quin2 fluorescence. The phorbol ester phorbol-12-myristate-13-acetate (PM...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80345-9
更新日期:1987-08-31 00:00:00
abstract::Prokaryotic aminoacylated-transfer RNAs often need to be efficiently segregated between translation and other cellular biosynthetic pathways. Many clinically relevant bacteria, including Streptococcus pneumoniae, Staphylococcus aureus, Enterococcus faecalis and Pseudomonas aeruginosa direct some aminoacylated-tRNA spe...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2013.07.036
更新日期:2013-09-17 00:00:00
abstract::The TSH-induced cyclic AMP response was studied using a 3-year-old ovine thyroid cell line TSH-independent for growth: OVNIS 5H. The kinetics of cyclic AMP production was followed both in cell layers and in cell culture media, with or without phosphodiesterase inhibitor. It is noteworthy that following the first wave ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80102-8
更新日期:1986-01-06 00:00:00
abstract::Hematopoietic progenitors have been shown to retain plasticity and switch lineages by appropriate stimuli. However, mature blood cells hardly showed such differentiation plasticity. In this paper, we tried to reprogram mature B cells into erythroid lineage by expressing various hematopoietic transcription factors. Amo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.08.019
更新日期:2012-10-19 00:00:00
abstract::It has been suggested that the C-terminal domain of Bcl-2 family members may contain a signal anchor sequence that targets these proteins to the mitochondrial outer membrane. We have investigated the consequence of deleting this domain upon cytochrome c release in yeast strains that coexpress truncated forms of Bax (i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01661-5
更新日期:1999-01-25 00:00:00
abstract::To evaluate ozone damage to hairless mouse skin, two parameters of oxidative damage, vitamin E depletion and malondialdehyde (MDA) production, were measured in vitamin E-enriched and in control skin from mice exposed to ozone (10 ppm). A 5% vitamin E solution (tocotrienol-rich fraction, TRF) in polyethylene glycol (PE...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01463-9
更新日期:1997-01-20 00:00:00
abstract::Localisation of mRNA encoding the four AMPA glutamate receptor subunits in the rat adrenal gland was investigated using in situ hybridisation. GluR1 is found in the zona glomerulosa of the cortex, GluR3 in the remaining parts of the cortex, GluR2 in adrenal medullary cells and GluR4 at a very low level in the zona glo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80472-7
更新日期:1993-10-11 00:00:00
abstract::The mitochondrial outer membrane contains a protein complex with at least eight subunits responsible for recognition and translocation of preproteins synthesized in the cytosol. Two subunits, the receptors Tom20 and Tom70, contain tetratrico peptide repeats that are thought to be involved in protein-protein interactio...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00156-1
更新日期:1996-03-11 00:00:00
abstract::Small RNAs are non-coding regulatory RNA molecules that control gene expression by mediating mRNA degradation, translational inhibition, or chromatin modification. Virus-derived small RNAs induce silencing of viral RNAs and are essential for antiviral defense in both animal and plant systems. The role of host endogeno...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2008.06.053
更新日期:2008-08-06 00:00:00
abstract::The kinetic properties of dUTPase from equine infectious anemia virus (EIAV) were investigated. K(M) (1.1 +/- 0.1 microM) and k(cat) (25 s(-1)) were found to be independent of pH in the neutral pH range. Above pH 8.0, K(M) increases slightly. Below pH 6.0, the enzyme is rapidly deactivated. Detergent was found to enha...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00935-6
更新日期:1997-09-08 00:00:00
abstract::Hterologous complementation of yeast mutants has enabled the isolation of genes encoding several families of amino acid transporters. Among them, NTR1 codes for a membrane protein with weak histidine transport activity. However, at the sequence level, NTR1 is related to rather non-specific oligopeptide transporters fr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00853-2
更新日期:1995-08-21 00:00:00
abstract::In human brain the Abeta peptide is produced mainly by neurons and the overexpression of amyloid precursor protein (APP) that involves an increase in Abeta secretion, has been observed in some areas of the Alzheimer's disease patients brain. We have generated two stably transfected human neuroblastoma lines which over...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.06.025
更新日期:2004-07-16 00:00:00
abstract::Fructose-1,6-bisphosphatase, a key enzyme in gluconeogenesis, undergoes catabolite inactivation when glucose is added to gluconeogenetically active cells of the yeast Saccharomyces cerevisiae. Phosphorylation of the enzyme is followed by rapid degradation. To elucidate the cellular proteolytic system involved in catab...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00668-7
更新日期:1994-08-01 00:00:00
abstract::The gap junctional intercellular communication mediated by Cx43 plays indispensable roles in both germ line development and postnatal folliculogenesis. In this study, we focused on the effect of follicle-stimulating hormone (FSH) on the Cx43 protein in rat primary granulosa cells and found that FSH stimulation elevate...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03441-5
更新日期:2002-11-06 00:00:00
abstract::Anandamide (AEA) exhibits anti-inflammatory effects. However, its role in the periodontal field remains unknown. Here, we found that gingival crevicular fluid contained a detectable level of AEA. The cannabinoid receptors CB1 and CB2 were expressed by human gingival fibroblasts (HGFs), and markedly upregulated under p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.12.079
更新日期:2006-01-23 00:00:00
abstract::We have observed that the extracellular domain of T beta RI and protectin (CD59), an inhibitor of the membrane attack complex of complement, share structural features, a distinct spacing of ten cysteines and a C-terminal 'Cys-box'. Based on these common features and the recently determined NMR-structure of protectin, ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01239-7
更新日期:1995-11-27 00:00:00
abstract::We investigated transactivation by NANOG in regulating growth and differentiation factor 3 (GDF3) expression in NCCIT cells. GDF3 expression was affected by shRNA-mediated downregulation and by exogenous overexpression of NANOG specifically, as well as by retinoic acid-mediated differentiation. GDF3 transcription was ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.08.013
更新日期:2012-09-21 00:00:00
abstract::pMNEI, a single chain sweet protein related to monellin, has been studied by means of 1H NMR at 500 MHz. A partial sequential assignment performed by means of the MCD method allowed the determination of the secondary structure of a large portion of the beta-sheet of pMNEI that contains a likely 'sweet finger': the loo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81138-c
更新日期:1992-09-21 00:00:00