Abstract:
:Evidence is presented that single electron reduction is sufficient for rapid electron transfer (k greater than 20 s-1 at pH 8.0 in 0.43 M potassium EDTA) between haem a/CuA and the binuclear centre in 'fast' oxidase, whereas in 'slow' oxidase intramolecular electron transfer is slow even when both CuA and haem a are reduced (k congruent to 0.01 s-1). However, while a single electron can equilibrate rapidly between CuA, haem a and CuB in 'fast' oxidase, it seems that equilibration with haem a3 is relatively slow (k congruent to 2 s-1). Electron transfer between cytochrome c and CuA/haem a is similar for both types of enzyme (k congruent to 2.4 x 10(5) M-1.s-1).
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Moody AJ,Brandt U,Rich PRdoi
10.1016/0014-5793(91)81161-zsubject
Has Abstractpub_date
1991-11-18 00:00:00pages
101-5issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(91)81161-Zjournal_volume
293pub_type
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