ADP-ribosylation of Rho enhances actin polymerization-coupled shape oscillations in human neutrophils.

abstract：:Activities of Escherichia coli DNA polymerase-I were examined in the presence of the anti-tumor drug cis-diaminedichloroplatinum(II) and its inactive geometric isomer trans-diaminedichloroplatinum(II). The trans-isomer did not inhibit the enzyme activity. The anti-tumor drug, on the other hand, retarded the enzyme in ...

journal_title：FEBS letters

authors： Duman RK,Heath RT,Bose RN

更新日期：1999-07-16 00:00:00

abstract：:Statistically significant similarity was revealed between amino acid sequences of NTP-binding pattern-containing domains which are among the most conserved protein segments in dissimilar groups of ss and dsDNA viruses (papova-, parvo-, geminiviruses and P4 bacteriophage), and RNA viruses (picorna-, como- and nepovirus...

journal_title：FEBS letters

authors： Gorbalenya AE,Koonin EV,Wolf YI

更新日期：1990-03-12 00:00:00

abstract：:Temperature-jump measurements were carried out on myosin subfragment 1 (S1) labeled at Cys-707 with 5-(iodoacetamido)fluorescein (S1-AF). The relaxation was monitored by following the increase in the fluorescence intensity of the attached probe after a jump of 5.8 degrees C. A single relaxation process was observed ov...

journal_title：FEBS letters

authors： Lin SH,Cheung HC

更新日期：1992-06-15 00:00:00

abstract：:We have shown by electrophoretic mobility shift assays that the nucleotide sequence CE-LPH1, centred at position -49 with respect to the transcription start site of the rat gene encoding intestinal lactase-phlorizin hydrolase, interacts in vitro with nuclear proteins present in the jejunum of suckling animals. Protein...

journal_title：FEBS letters

authors： Boukamel R,Freund JN

更新日期：1994-10-10 00:00:00

abstract：:cDNAs encoding aquaporins PIP1;1, PIP2;1, and TIP1;1 were isolated from Mimosa pudica (Mp) cDNA library. MpPIP1;1 exhibited no water channel activity; however, it facilitated the water channel activity of MpPIP2;1 in a phosphorylation-dependent manner. Mutagenesis analysis revealed that Ser-131 of MpPIP1;1 was phospho...

journal_title：FEBS letters

authors： Temmei Y,Uchida S,Hoshino D,Kanzawa N,Kuwahara M,Sasaki S,Tsuchiya T

更新日期：2005-08-15 00:00:00

abstract：:In a previous investigation of the rDNA region in Tetrahymena pyriformis mitochondrial DNA, we identified a putative tRNA(Met) gene [Heinonen et al. (1987) J. Biol. Chem. 262, 2879-2887]. On the basis of Northern hybridization analyses, we suggested that this gene is expressed, even though the resulting tRNA would be ...

journal_title：FEBS letters

authors： Schnare MN,Greenwood SJ,Gray MW

更新日期：1995-03-27 00:00:00

abstract：:Photosystem I (PSI) complex of Anabaena variabilis ATCC 29413 consists of at least 11 subunits, 9 of which are resolved by high resolution gel electrophoresis. N-terminal amino acid sequences of the four subunits with molecular masses of 6.8, 5.2, 4.8 and 3.5 kDa were determined. Based on the sequence homology, the 3....

journal_title：FEBS letters

authors： Ikeuchi M,Nyhus KJ,Inoue Y,Pakrasi HB

更新日期：1991-08-05 00:00:00

abstract：:We have followed with autoradiography the course of DNA synthesis and with cytofluorometry the partition of DNA during mitosis, through the different phases of the life-span of human fibroblasts. Towards the end of the proliferative potential of the cell population, DNA synthesis was disturbed during the late part of ...

journal_title：FEBS letters

authors： Macieira-Coelho A

更新日期：1995-01-23 00:00:00

abstract：:LLC-PK1L cells, a kidney-derived cell line grown in defined medium, possess a vasopressin-sensitive adenylate cyclase. Somatostatin was able to inhibit the vasopressin-induced increase in adenylate cyclase activity, without affecting the basal enzyme activity. This inhibition was competitive. No effect of somatostatin...

journal_title：FEBS letters

authors： Roy C

更新日期：1984-04-24 00:00:00

abstract：:Three site-directed mutants of human epidermal growth factor, Leu-26----Gly, Leu-47----Ala, and Ile-23----Thr, were examined for their ability to stimulate the protein-tyrosine kinase activity of the epidermal growth factor receptor. The receptor binding affinities of the mutant growth factors were 20- to 50-fold lowe...

journal_title：FEBS letters

authors： Matsunami RK,Campion SR,Niyogi SK,Stevens A

更新日期：1990-05-07 00:00:00

abstract：:Ample evidence indicates that sphingosine-1-phosphate (SPP) can serve as an intracellular second messenger regulating calcium mobilization, and cell growth and survival. Moreover, the dynamic balance between levels of the sphingolipid metabolites, ceramide and SPP, and consequent regulation of opposing signaling pathw...

journal_title：FEBS letters

authors： Spiegel S,Milstien S

更新日期：2000-06-30 00:00:00

abstract：:There is strong evidence of a key role for mitochondrial dysfunction in both sporadic and all forms of familial Parkinson's disease (PD). However, none of the clinical trials carried out with putative mitochondrial rescue agents have been successful. Firm establishment of a wet biomarker or a reliable readout from ima...

journal_title：FEBS letters

authors： Mortiboys H,Macdonald R,Payne T,Sassani M,Jenkins T,Bandmann O

更新日期：2018-03-01 00:00:00

abstract：:Glucosamine-6-phosphate (GlcN6P) N-acetyltransferase 1 (GNA1) is a key enzyme in the pathway toward biosynthesis of UDP-N-acetylglucosamine, an important donor substrate for N-linked glycosylation. GNA1 catalyzes the formation of N-acetylglucosamine-6-phosphate (GlcNAc6P) from acetyl-CoA (AcCoA) and the acceptor subst...

journal_title：FEBS letters

authors： Wang J,Liu X,Liang YH,Li LF,Su XD

更新日期：2008-09-03 00:00:00

abstract：:Using time-resolved low-temperature spectroscopy, we have examined whether or not bovine rhodopsin has a unique transducin-binding state, meta Ib, previously detected from chicken rhodopsin. Unlike chicken meta Ib, bovine meta Ib was detected only by detailed kinetics analysis of the bleaching process, but it was stab...

journal_title：FEBS letters

authors： Tachibanaki S,Imai H,Terakita A,Shichida Y

更新日期：1998-03-20 00:00:00

abstract：:The hypothesis that specific protein kinase C (PKC) isoforms regulate dopamine transporter (DAT) function was tested in Xenopus laevis oocytes expressing human (h)DAT. Activation of conventional PKCs (cPKCs) and novel PKCs (nPKCs) using 10 nM phorbol 12-myristate 13-acetate (PMA) significantly inhibited DAT-associated...

journal_title：FEBS letters

authors： Doolen S,Zahniser NR

更新日期：2002-04-10 00:00:00

abstract：:Two free radical scavengers, bioflavonoid rutin and the copper-rutin complex Cu(Rut)Cl2, inhibited lucigenin-amplified chemiluminescence and lipid peroxidation in rat liver microsomes, Cu(Rut)Cl2 being a 5-9 times more efficient inhibitor than rutin. The enhanced inhibitory activity of Cu(Rut)Cl2 was due to the presen...

journal_title：FEBS letters

authors： Afanas'ev IB,Ostrachovich EA,Korkina LG

更新日期：1998-03-27 00:00:00

abstract：:Linkage between the high-capacity Ca2+-binding protein calsequestrin and the ryanodine receptor is proposed to be essential for proper Ca2+-release during skeletal muscle excitation-contraction coupling. However, no direct biochemical evidence exists showing a connection between these high-molecular-mass complexes in ...

journal_title：FEBS letters

authors： Murray BE,Ohlendieck K

更新日期：1998-06-16 00:00:00

abstract：:The eukaryotic Ssu72 factor is involved in several RNA biogenesis processes. It has phosphatase activity on the carboxy-terminal domain (CTD) of the major subunit of RNA polymerase II. The Kluyveromyces lactis Ssu72 (KlSsu72) shows in vitro phosphatase activity for the pNPP substrate, and this activity is inhibited by...

journal_title：FEBS letters

authors： Rodríguez-Torres AM,Lamas-Maceiras M,García-Díaz R,Freire-Picos MA

更新日期：2013-08-19 00:00:00

abstract：:Escherichia coli outer membrane protease OmpT has been characterised as a serine protease based on its inhibitor profile, but serine protease consensus sequences are absent. By site-directed mutagenesis we substituted all conserved serines and histidines. Substitution of His(101) and His(212) by Ala, Asn or Gln result...

journal_title：FEBS letters

authors： Kramer RA,Dekker N,Egmond MR

更新日期：2000-02-25 00:00:00

abstract：:ADP-ribosylation of HeLa nonhistone proteins was investigated by using [3H]adenosine as an in vivo radioactive label. The aim was to determine basic differences in the patterns of modification of interphase and metaphase nonhistones. Fluorography revealed a relatively small number of modified proteins for isolated met...

journal_title：FEBS letters

authors： Adolph KW,Song MK

更新日期：1985-03-11 00:00:00

abstract：:A major house dust mite allergen Der f 1 belongs to the papain-like cysteine protease family. This study investigated whether Der f 1 can cleave the latency-associated peptide (LAP) of transforming growth factor (TGF)-beta via its proteolytic activity and activate latent TGF-beta. We found that Der f 1 can cleave LAP ...

journal_title：FEBS letters

authors： Nakamura Y,Miyata M,Shimokawa N,Ohnuma Y,Katoh R,Ogawa H,Okumura K,Nakao A

更新日期：2009-06-18 00:00:00

abstract：:Despite significant increases in circulating concentrations of lipid fuels (triacylglycerol, non-esterified fatty acids (NEFA) and ketone bodies) in late-pregnant rats sampled in the fed (absorptive) state, cardiac and skeletal muscle active pyruvate dehydrogenase (PDHa) activities remained comparable with those obser...

journal_title：FEBS letters

authors： Sugden MC,Holness MJ

更新日期：1993-04-26 00:00:00

abstract：:The activity of the Ca-pumping ATPase of cardiac sarcoplasmic reticulum (SR) is controlled by the phosphorylation of the intrinsic regulatory protein phospholamban (PLB), which affects both the apparent Km(Ca) and the Vmax of the transport process. We have investigated the correlation between phosphorylation of PLB an...

journal_title：FEBS letters

authors： Chiesi M,Schwaller R

更新日期：1989-02-13 00:00:00

abstract：:Biological activities of five synthetic lipid A analogues (D-glucosamine derivatives) were examined to elucidate the structure required for expression of the biological activities of endotoxin. Proclotting enzyme of horseshoe crab activation, interferon-inducing and tumor necrosis factor-inducing activities were signi...

journal_title：FEBS letters

authors： Matsuura M,Kojima Y,Homma JY,Kubota Y,Yamamoto A,Kiso M,Hasegawa A

更新日期：1984-02-27 00:00:00

abstract：:FT-IR analysis shows that treatment of F1ATPase with the inhibitors DCCD and Nbf-Cl, in the presence of saturating concentrations of ADP and AMP-PNP and in the absence of Mg2+, does not modify the secondary structure of the enzyme, but significantly modifies its compactness and thermal stability, although to different...

journal_title：FEBS letters

authors： Lippe G,Tanfani F,Di Pancrazio F,Contessi S,Bertoli E,Dabbeni-Sala F

更新日期：1998-08-07 00:00:00

abstract：:Human ovarian clear cell carcinoma cell line (transferrin (Tf)-non-producer), HAC 2, cells were adapted to grow in chemically defined synthetic medium when the cells were cultured with medium containing 10 micrograms/ml of insulin at least for 6 months. They synthesized and secreted constantly the 80 kDa protein immun...

journal_title：FEBS letters

authors： Ohkawa K,Takada K,Takizawa N,Hatano T,Tsukada Y,Matsuda M

更新日期：1990-09-17 00:00:00

abstract：:Caveolin is a protein present in membrane specializations termed caveolae where it may play a structural role. Previous studies on the cDNA sequence of chicken caveolin demonstrated that it is an integral membrane protein without significant homology to any known protein. The cDNA sequence of human lung caveolin is pr...

journal_title：FEBS letters

authors： Glenney JR Jr

更新日期：1992-12-07 00:00:00

abstract：:Trefoil factor family (TFF)-domain peptides 1-3 are mucin-associated molecules, largely found in epithelia of gastrointestinal tissues. Structurally similar, resistant to enzymatic degradation, they are up-regulated around areas of epithelial damage such as ulcers. Transgenic expression or exogenous peptide ameliorate...

journal_title：FEBS letters

authors： Wright NA,Hoffmann W,Otto WR,Rio MC,Thim L

更新日期：1997-05-19 00:00:00

abstract：:Upon dimerization by oxidation, Hsp33 functions as a molecular chaperone in prokaryotes. Previously published structures of both the inactive and active species are of doubtful relevance to the solution conformations since the inactive (reduced) crystal structure was dimeric, while the active (oxidized) species was cr...

journal_title：FEBS letters

authors： Lee YS,Ryu KS,Kim SJ,Ko HS,Sim DW,Jeon YH,Kim EH,Choi WS,Won HS

更新日期：2012-02-17 00:00:00

abstract：:Glucose-fructose oxidoreductase (GFOR), a periplasmic protein of Zymomonas mobilis, is synthesized as a precursor polypeptide with a twin-R signal peptide for Sec-independent protein export in bacteria. In higher plant chloroplasts, twin-R signal peptides are specific targeting signals for the Sec-independent delta pH...

journal_title：FEBS letters

authors： Halbig D,Hou B,Freudl R,Sprenger GA,Klösgen RB

更新日期：1999-03-19 00:00:00