Abstract:
:Regulators of G protein signaling RGS4 and RGS7 accelerate the kinetics of K(+) channels (GIRKs) in the Xenopus oocyte system. Here, via quantitative analysis of RGS expression, we reveal biphasic effects of RGSs on GIRK regulation. At low concentrations, RGS4 inhibited basal GIRK activity, but stimulated it at high concentrations. RGS7, which is associated with the G protein subunit G beta 5, is regulated by G beta 5 by two distinct mechanisms. First, G beta 5 augments RGS7 activity, and second, it increases its expression. These dual effects resolve previous controversies regarding RGS4 and RGS7 function and indicate that they modulate signaling by mechanisms supplementary to their GTPase-activating protein activity.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Keren-Raifman T,Bera AK,Zveig D,Peleg S,Witherow DS,Slepak VZ,Dascal Ndoi
10.1016/s0014-5793(01)02220-7keywords:
subject
Has Abstractpub_date
2001-03-09 00:00:00pages
20-8issue
1-2eissn
0014-5793issn
1873-3468pii
S0014-5793(01)02220-7journal_volume
492pub_type
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