Thiostrepton binds to malarial plastid rRNA.

abstract：:Chimeric antibodies composed of mouse-derived variable regions and human-derived constant regions have been developed for clinical use. However, construction of chimeric mouse/human genes in expression vectors is time-consuming work. In this study, we developed convenient vectors for construction of chimeric mouse/hum...

journal_title：FEBS letters

authors： Kameyama K,Imai K,Itoh T,Taniguchi M,Miura K,Kurosawa Y

更新日期：1989-02-27 00:00:00

abstract：:By use of the patch clamp technique, we have compared the electrophysiological signature of OmpC porin channels at neutral and acidic pH. The perfusion of pH 5.4 buffer to the periplasmic side of excised patches promoted the closure or block of approximately 20% of the open porins present in the patch without changes ...

journal_title：FEBS letters

authors： Liu N,Delcour AH

更新日期：1998-08-28 00:00:00

abstract：:Osteoblastic cells have been shown to be involved in osteoclast formation through cell to cell contacts. This study was designed to examine the possible function of vascular cell adhesion molecule 1 (VCAM-1) during osteoclastogenesis. As a source for stromal cells we used the recently established mouse bone marrow str...

journal_title：FEBS letters

authors： Feuerbach D,Feyen JH

更新日期：1997-01-27 00:00:00

abstract：:Nine analogs of fusion peptide of influenza virus hemagglutinin whose membrane perturbation activity has been thoroughly tested [Murata et al. (1992) Biochemistry 31, 1986-1992; Murata et al. (1993) Biophys. J. 64, 724-734] were characterized by molecular modeling techniques with the aim of delineating any specific st...

journal_title：FEBS letters

authors： Efremov RG,Nolde DE,Volynsky PE,Chernyavsky AA,Dubovskii PV,Arseniev AS

更新日期：1999-11-26 00:00:00

abstract：:Human nuclear cyclophilin 33 (hCyP33) was the first protein which was found to contain an RNA-binding motif and a PPIase domain. It was not known what cellular and physiological roles are played by the RNA-binding activity as well as the PPIase activity of hCyP33. In this paper, we investigated the binding specificity...

journal_title：FEBS letters

authors： Wang Y,Han R,Zhang W,Yuan Y,Zhang X,Long Y,Mi H

更新日期：2008-03-05 00:00:00

abstract：:Transcription mediators are evolutionarily conserved from yeast to human. We previously reported the specific in vivo roles of mediators during development. Transcriptional mediators including med-6, med-7, and med-10 were shown to be involved in the regulated transcription of specific genes, but not in the transcript...

journal_title：FEBS letters

authors： Kwon JY,Kim-Ha J,Lee BJ,Lee J

更新日期：2001-11-23 00:00:00

abstract：:Hypercellularity and accumulation of extracellular matrix are common responses of renal glomeruli to inflammatory stimuli. Using the differential display approach, we compared the gene expression patterns of proliferating and differentiating rat mesangial cells in two- and three-dimensional cultures. Osteopontin, an e...

journal_title：FEBS letters

authors： Pröls F,Heidgress D,Rupprecht HD,Marx M

更新日期：1998-01-23 00:00:00

abstract：:Using the patch-clamp technique, we demonstrate here the opening of K+ channels evoked by the actively transported amino acid L-alanine in isolated Necturus enterocytes. These channels had a conductance of about 30 pS and their activation was dependent on transmembrane electrical potential and cytosolic Ca2+. ...

journal_title：FEBS letters

authors： Sheppard DN,Giraldez F,Sepúlveda FV

更新日期：1988-07-18 00:00:00

abstract：:Phosphorylation of H2AX is believed to be associated with the repair of damaged DNA. Recent findings suggest a novel function of H2AX in cellular apoptosis. Specifically, it was shown that ultraviolet A-activated JNK phosphorylates H2AX to regulate apoptosis. Here we show that serum starvation induces H2AX phosphoryla...

journal_title：FEBS letters

authors： Lu C,Shi Y,Wang Z,Song Z,Zhu M,Cai Q,Chen T

更新日期：2008-08-06 00:00:00

abstract：:The Fanconi anemia (FA) pathway, of which the FANCD2 protein is a key component, plays crucial roles in the maintenance of hematopoietic stem cells and suppression of carcinogenesis. However, the function of FANCD2 remains unclear. Here, we report that FANCD2 is a novel and specific substrate of caspase 3. Cleavage of...

journal_title：FEBS letters

authors： Sakai W,Sugasawa K

更新日期：2014-10-16 00:00:00

abstract：:The ability of (S)-alpha-methylproline (alpha-MePro) to stabilise reverse-turn conformations in the peptide hormone bradykinin (BK = Arg1-Pro2-Pro3-Gly4-Phe5-Ser6-Pro7-Phe8-Arg9) has been investigated. Two BK analogues containing alpha-MePro at position 3 or position 7 were synthesised and their conformations in aqueo...

journal_title：FEBS letters

authors： Welsh JH,Zerbe O,von Philipsborn W,Robinson JA

更新日期：1992-02-10 00:00:00

abstract：:Mitochondrial ATP synthase, a major ATP supplier in respiring cells, should be regulated in amount and in activity to respond to the varying demands of cells for ATP. We screened 80 protein kinase inhibitors and found that HeLa cells treated with four inhibitors exhibited reduced mitochondrial ATP synthesis activity. ...

journal_title：FEBS letters

authors： Sugawara K,Fujikawa M,Yoshida M

更新日期：2013-11-29 00:00:00

abstract：:Carbohydrate chains of C1-inhibitor were identified with a binding assay using different lectins. Lectins from Sambucus nigra (SNA) and Maackia amurensis (MAA) that are specific for sialic acids bound to C1-inhibitor. Lectin from Datura stramonium (DSA) reacted also with the inhibitor indicating complex and hybrid sug...

journal_title：FEBS letters

authors： Schoenberger OL

更新日期：1992-12-21 00:00:00

abstract：:The fraction of polyclonal caldesmon antibodies cross-reacting with rabbit skeletal troponin T are shown to compete with smooth muscle tropomyosin for caldesmon and troponin T, as revealed by ELISA method. The epitope recognized by these antibodies was also found in Mr 77 kDa non-muscle caldesmon. These results provid...

journal_title：FEBS letters

authors： Birukov KG,Shirinsky VP,Vorotnikov AV,Gusev NB

更新日期：1990-03-26 00:00:00

abstract：:Biochemical, immunohistochemical and molecular biological methods were used to detect fetal myosin heavy chain (MyHC) in the human masseter of elderly and young subjects. Samples from the elderly subjects contained larger amounts of fetal MyHC than those of young adults. Only a very small amount of embryonic MyHC coul...

journal_title：FEBS letters

authors： Monemi M,Eriksson PO,Dubail I,Butler-Browne GS,Thornell LE

更新日期：1996-05-13 00:00:00

abstract：:Azotobacter vinelandii (Av) and chroococcum (Ac) ferredoxin I contain [3Fe-4S]1 + 0 and [4Fe-4S]2+1+ clusters, when isolated aerobically, which undergo one-electron redox cycles at potentials of -460 +/- 10 mV (vs SHE) at pH 8.3 and -645 +/- 10 mV, respectively. The X-ray structure of Fd I (Av) reveals that the N-term...

journal_title：FEBS letters

authors： Thomson AJ

更新日期：1991-07-22 00:00:00

abstract：:The structure of the complex of Ras with the Ras-binding domain of its effector RalGDS (RGS-RBD), the first genuine Ras-effector complex, has been solved by X-ray crystallography. As with the Rap-RafRBD complex (Nasser et al., 1995), the interaction is via an inter-protein beta-sheet between the switch I region of Ras...

journal_title：FEBS letters

authors： Vetter IR,Linnemann T,Wohlgemuth S,Geyer M,Kalbitzer HR,Herrmann C,Wittinghofer A

更新日期：1999-05-21 00:00:00

abstract：:Tert-butyl hydroperoxide (BHP), hydrogen peroxide and diamide caused a rapid and simultaneous release of glutathione disulfide (GSSG) and K+ in the isolated perfused rat liver. Both BHP-induced effluxes were suppressed by prior depletion of hepatic glutathione, but not by co-infusion of desferrioxamine which prevented...

journal_title：FEBS letters

authors： Masuda Y,Ozaki M,Aoki S

更新日期：1993-11-08 00:00:00

abstract：:The somatostatin upstream enhancer (SMS-UE) is a highly complex enhancer element. The distal A-element contains overlapping Pdx1 and Pbx binding sites. However, a point mutation in the A-element that abolishes both Pdxl and Pbx binding does not impair promoter activity. In contrast, a point mutation that selectively e...

journal_title：FEBS letters

authors： Andersen FG,Jensen J,Heller RS,Petersen HV,Larsson LI,Madsen OD,Serup P

更新日期：1999-02-26 00:00:00

abstract：:The relationship between glutathione deficiency, glycogen metabolism and ascorbate synthesis was investigated in isolated murine hepatocytes. Glutathione deficiency caused by various agents increased ascorbate synthesis with a stimulation of glycogen breakdown. Increased ascorbate synthesis from UDP-glucose or gulonol...

journal_title：FEBS letters

authors： Braun L,Csala M,Poussu A,Garzó T,Mandl J,Bánhegyi G

更新日期：1996-06-17 00:00:00

abstract：:32 consensus patterns for a set of functional regions and structural motifs in protein sequences were constructed. The pattern definition is heuristic and based on 11 selected steric and physicochemical properties. By comparison with these patterns, it was possible to identify, without false detection, 1532 sites in 8...

journal_title：FEBS letters

authors： Bork P

更新日期：1989-10-23 00:00:00

abstract：:Feeding insects introduce oral secretions (OS) into the wounded tissue of the attacked plant. Various OS-derived molecules must be involved in subsequent processes including the induction of plant defence reactions. Using the planar lipid bilayer membrane technique, isolated OS were analyzed with respect to their memb...

journal_title：FEBS letters

authors： Maischak H,Grigoriev PA,Vogel H,Boland W,Mithöfer A

更新日期：2007-03-06 00:00:00

abstract：:Incubation of phosphatidylethanolamine containing liposomes with malondialdehyde and other aldehydes with different chain lengths results in the fluorescence of chromolipids. Relative to malondialdehyde, the fluorescence was greatly enhanced with increasing chain length upon incubation of 2-alkenals with phospholipids...

journal_title：FEBS letters

authors： Montfoort A,Bezstarosti K,Groh MM,Koster JF

更新日期：1987-12-21 00:00:00

abstract：:It is well known that, in rabbits, the allotypes of the b locus carried by the light chains of the IgG molecules cross-react. Nothing similar has been described for the allotypes of the a locus carried by the heavy chains of the IgG molecules. This paper describes a cross-reaction between the rabbit allotypic markers ...

journal_title：FEBS letters

authors： Renneboog-Squilbin C

更新日期：1969-02-01 00:00:00

abstract：:FixK₂ is a CRP-like transcription factor that controls the endosymbiotic lifestyle of Bradyrhizobium japonicum. The reason for its noticeable protease sensitivity was explored here. The repertoire of Clp chaperone-proteases in B. japonicum was examined, and specifically ClpAP₁ and ClpXP₁ were purified and tested. FixK...

journal_title：FEBS letters

authors： Bonnet M,Stegmann M,Maglica Ž,Stiegeler E,Weber-Ban E,Hennecke H,Mesa S

更新日期：2013-01-04 00:00:00

abstract：:This paper summarizes results of the 40-year studies on replication and recombination of RNA molecules in the cell-free amplification system of bacteriophage Q. Special attention is paid to the molecular colony technique that has provided for the discovery of the nature of "spontaneous" RNA synthesis by Q replicase an...

journal_title：FEBS letters

authors： Chetverin AB

更新日期：2004-06-01 00:00:00

abstract：:Mitogen-activated protein (MAP) kinases p42mapk and p44mapk are activated by dual tyrosine and threonine phosphorylation in vivo. Both MAPKs are phosphorylated and activated in vitro by an activator recently identified as a protein-tyrosine/threonine kinase. We have isolated a putative cDNA for a MAP kinase kinase (MA...

journal_title：FEBS letters

authors： Haystead CM,Wu J,Gregory P,Sturgill TW,Haystead TA

更新日期：1993-02-08 00:00:00

abstract：:The requirement for a proline residue at the C-terminus of the Ca(2+)-binding photoprotein, aequorin, was investigated by measuring luminescence activities of a series of C-terminal deletion mutants, substitution mutants and an addition mutant. CD spectral measurements of apoaequorin with the C-terminal proline delete...

journal_title：FEBS letters

authors： Nomura M,Inouye S,Ohmiya Y,Tsuji FI

更新日期：1991-12-16 00:00:00

abstract：:Mouse epidermal cytosol contains a protein phosphatase with Mr 38,000, which dephosphorylates the elongation factor 2 (EF-2) of protein biosynthesis and is stimulated after topical application of TPA to mouse skin [(1988) Biochem. Biophys. Res. Commun. 153, 1129-1135]. Dephosphorylation of EF-2 by this phosphatase is ...

journal_title：FEBS letters

authors： Gschwendt M,Kittstein W,Mieskes G,Marks F

更新日期：1989-11-06 00:00:00

abstract：:Using a BAL31 exonuclease assay to determine the sites of 4,5',8-trimethylpsoralen photocrosslinking in DNA we have shown that 5'-TA sites which are accessible to psoralen DNA interstrand photocrosslinking in naked DNA become inaccessible when protein, in casu, lambda-repressor E. coli or RNA polymerase are bound at t...

journal_title：FEBS letters

authors： Zhen WP,Jeppesen C,Nielsen PE

更新日期：1988-02-29 00:00:00