Structure of dimeric ATP synthase from mitochondria: an angular association of monomers induces the strong curvature of the inner membrane.

abstract：:It was of interest to examine whether yeast Cu-thionein could be used to transfer the thiolate bound copper directly into the copper binding site of 'blue' apoproteins which contain free thiol groups. In particular apo-stellacyanin was used in the present study and it was found to be able to accept Cu(I) from yeast Cu...

journal_title：FEBS letters

authors： Hartmann HJ,Morpurgo L,Desideri A,Rotilio G,Weser U

更新日期：1983-02-07 00:00:00

abstract：:Oligopeptides derived from the gag polyprotein (Pr55gag) of human immunodeficiency virus type 1 (HIV-1) segment were used to evaluate the extension of the putative binding region for the complex of Pr55gag and the human cytosolic peptidyl prolyl cis/trans isomerase (PPIase) 18 kDa cyclophilin (Cyp18). Five N-terminall...

journal_title：FEBS letters

authors： Schutkowski M,Drewello M,Wöllner S,Jakob M,Reimer U,Scherer G,Schierhorn A,Fischer G

更新日期：1996-10-07 00:00:00

abstract：:The R2 protein component of mouse ribonucleotide reductase has been obtained from overproducing Escherichia coli bacteria. It has been crystallized using NaCl as precipitant. The crystals are orthorhombic, space group C222(1), with cell dimensions a = 76.9 A, b = 108.9 A, c = 92.7 A and diffract to at least 2.5 A. The...

journal_title：FEBS letters

authors： Nielsen BB,Kauppi B,Thelander M,Thelander L,Larsen IK,Eklund H

更新日期：1995-10-16 00:00:00

abstract：:The oleosin glycine-rich protein genes Atgrp-6, Atgrp-7, and Atgrp-8 occur in clusters in the Arabidopsis genome and are expressed specifically in the tapetum cells. The cis-regulatory regions involved in the tissue-specific gene expression were investigated by fusing different segments of the gene cluster to the uidA...

journal_title：FEBS letters

authors： Franco LO,de O Manes CL,Hamdi S,Sachetto-Martins G,de Oliveira DE

更新日期：2002-04-24 00:00:00

abstract：:Phosphatidylinositol transfer proteins (PITP) are abundant cytosolic proteins originally identified because of their ability to act in vitro as specific transporters of phosphatidylinositol or phosphatidylcholine between membranes. However, the cellular function of mammalian PITP has remained enigmatic till recently. ...

journal_title：FEBS letters

authors： Cockcroft S

更新日期：1997-06-23 00:00:00

abstract：:Sequence homology was found by computer analysis between potato spindle tuber viroid (PSTV) RNA and U3B snRNA of Novikoff hepatoma cells. This homology is colinear in arrangement, extends in length to 81% of the entire U3B snRNA molecule and is involved in the PSTV molecule unique sites which, if depicted in terms of ...

journal_title：FEBS letters

authors： Kiss T,Pósfai J,Solymosy F

更新日期：1983-11-14 00:00:00

abstract：:Rabbit red blood cells of various ages were separated on Percoll gradients and the activities of two large cytosolic proteases were measured. Both the multicatalytic protease (MCP), assayed by hydrolysis of fluorigenic peptides, and the 26 S ubiquitin/ATP-stimulated protease, assayed by degradation of ubiquitin-lysozy...

journal_title：FEBS letters

authors： Di Cola D,Pratt G,Rechsteiner M

更新日期：1991-03-11 00:00:00

abstract：:The band 3 protein-mediated, valinomycin-induced KCl efflux continues to increase with increasing [KCl] when the Cl-/Cl- equilibrium exchange becomes saturated. This suggests the existence of a band 3-mediated component of Cl- flux that contributes to the electrical conductance without being related to slippage; i.e.,...

journal_title：FEBS letters

authors： Kaplan JH,Pring M,Passow H

更新日期：1983-05-30 00:00:00

abstract：:The multidrug resistance protein (MRP) is an ATP-dependent transport protein for organic anions, as well as neutral or positively charged anticancer agents. In this study we report that dinitrophenyl-S-glutathione increases ATPase activity in plasma membrane vesicles prepared from the MRP-overexpressing cell line GLC4...

journal_title：FEBS letters

authors： Hooijberg JH,Broxterman HJ,Heijn M,Fles DL,Lankelma J,Pinedo HM

更新日期：1997-08-18 00:00:00

abstract：:Lipid transfer proteins (LTPs) and elicitins are both able to load and transfer lipidic molecules and share some structural and functional properties. While elicitins are known as elicitors of plant defence mechanisms, the biological function of LTP is still an enigma. We show that a wheat LTP1 binds with high affinit...

journal_title：FEBS letters

authors： Buhot N,Douliez JP,Jacquemard A,Marion D,Tran V,Maume BF,Milat ML,Ponchet M,Mikès V,Kader JC,Blein JP

更新日期：2001-11-30 00:00:00

abstract：:The ubiquitin-like modifier, FAT10, is involved in proteasomal degradation and antigen processing. As ubiquitin and the ubiquitin-like modifier, ISG15, cotranslationally modify proteins, we investigated whether FAT10 could also be conjugated to newly synthesized proteins. Indeed, we found that nascent proteins are mod...

journal_title：FEBS letters

authors： Spinnenhirn V,Bitzer A,Aichem A,Groettrup M

更新日期：2017-01-01 00:00:00

abstract：:The bipartite human interleukin-4 (IL-4) receptor was functionally expressed in murine pro-B cells and activated by human IL-4 to evoke intracellular signaling. Mutual association of signal transducing proteins within the receptor complex was then studied in dependence of ligand stimulation. Besides ligand-induced rec...

journal_title：FEBS letters

authors： Erhardt I,Lischke A,Sebald W,Friedrich K

更新日期：1998-11-13 00:00:00

abstract：:We analyzed the expression of aquaporins (AQPs) in the small intestine to elucidate their functions, and found that AQP9, which had not previously been detected there, is present in duodenum, jejunum, and ileum. AQP9 is expressed in colon as well, but not in stomach. Also, its expression in these intestinal sections i...

journal_title：FEBS letters

authors： Okada S,Misaka T,Matsumoto I,Watanabe H,Abe K

更新日期：2003-04-10 00:00:00

abstract：:We have studied the pharmacological properties of genetically engineered human NK1 tachykinin receptors in which residues at the extracellular surface of the fourth transmembranal domain were substituted with the corresponding amino acids from the NK2 receptor. We show that substitution of G166C:Y167F in the human NK1...

journal_title：FEBS letters

authors： Ciucci A,Palma C,Riitano D,Manzini S,Werge TM

更新日期：1997-10-27 00:00:00

abstract：:Piericidin is a potent inhibitor of the mitochondrial and bacterial type I NADH-ubiquinone oxidoreductases (Complex I) and is considered to bind at or close to the ubiquinone binding site(s) of the enzyme. Piericidin-resistant mutants of the bacterium Rhodobacter capsulatus have been isolated and the present work demo...

journal_title：FEBS letters

authors： Darrouzet E,Issartel JP,Lunardi J,Dupuis A

更新日期：1998-07-10 00:00:00

abstract：:Prochlorococcus marinus SS120 is an ecologically important and biochemically intriguing marine cyanobacterium. In addition to divinyl chlorophylls (DV-Chls) a and b it possesses a particular form of phycoerythrin (PE), but no other phycobilins and therefore no complete phycobilisomes. Here, a spectroscopic characteris...

journal_title：FEBS letters

authors： Steglich C,Mullineaux CW,Teuchner K,Hess WR,Lokstein H

更新日期：2003-10-09 00:00:00

abstract：:Nuclear ionic channels (NICs) represent ubiquitous structures of living cells, although little is known about their functional properties and encoding genes. To characterize NICs, liver nuclear membrane vesicles were reconstituted into either planar lipid bilayers or proteoliposomes. Reconstitution of nuclear envelope...

journal_title：FEBS letters

authors： Guihard G,Proteau S,Payet MD,Escande D,Rousseau E

更新日期：2000-07-07 00:00:00

abstract：:Rat peritoneal mast cells, labelled with [14C]arachidonic acid, released histamine and [14C]arachidonic acid upon the addition of A23187. The release induced by low concentrations of A23187 was suppressed by removal of extracellular Na+ and by addition of the Na+/H+ exchange inhibitor, amiloride. Addition of protein k...

journal_title：FEBS letters

authors： Linnebjerg H,Hansen HS,Jensen B

更新日期：1988-11-21 00:00:00

abstract：:We report that the rat beta 3-adrenergic receptor (beta 3-AR) gene has an intron. The intron starts with an in-frame stop codon with the result that unspliced transcripts will encode a C-terminal truncated protein. The reported protein sequences of mouse and human beta 3-AR were both deduced from genomic DNA sequences...

journal_title：FEBS letters

authors： Bensaid M,Kaghad M,Rodriguez M,Le Fur G,Caput D

更新日期：1993-03-08 00:00:00

abstract：:Streptomyces lividans 1326-9, a bldA+ strain, and its bldA39 mutant derivative J1725 were transformed with a cosmid containing the pur cluster, which determines the puromycin biosynthetic pathway from Streptomyces alboniger. bldA+ transformants produced puromycin in typical amounts, whereas bldA39 transformants did so...

journal_title：FEBS letters

authors： Tercero JA,Espinosa JC,Jiménez A

更新日期：1998-01-16 00:00:00

abstract：:Single-pass sequences were obtained from the 5'-ends of a total of 1794 rose petal cDNA clones. Cluster analysis identified 242 groups of sequences and 635 singletons indicating that the database represents a total of 877 genes. Putative functions could be assigned to 1151 of the transcripts. Expression analysis indic...

journal_title：FEBS letters

authors： Channelière S,Rivière S,Scalliet G,Szecsi J,Jullien F,Dolle C,Vergne P,Dumas C,Bendahmane M,Hugueney P,Cock JM

更新日期：2002-03-27 00:00:00

abstract：:The human mutT homolog, hMTH1, suppresses spontaneous mutations by degrading the endogeneous mutagen, 8-hydroxy-dGTP. We previously reported the broad substrate specificity of hMTH1, which also degrades the oxidatively damaged purine nucleotides, 2-hydroxy-dATP, 8-hydroxy-dATP, 2-hydroxy-ATP, and 8-hydroxy-GTP, in add...

journal_title：FEBS letters

authors： Fujikawa K,Yakushiji H,Nakabeppu Y,Suzuki T,Masuda M,Ohshima H,Kasai H

更新日期：2002-02-13 00:00:00

abstract：:The inhibitory glycine receptor (GlyR) is a ligand-gated chloride channel protein which displays developmental heterogeneity in the mammalian central nervous system. Here we describe 2 novel cDNA variants of the rat GlyR alpha 2 subunit and demonstrate that alternative splicing generates these 2 isoforms. The deduced ...

journal_title：FEBS letters

authors： Kuhse J,Kuryatov A,Maulet Y,Malosio ML,Schmieden V,Betz H

更新日期：1991-05-20 00:00:00

abstract：:Endothelial dysfunction plays a major role in the pathogenesis of atherosclerosis. Pro-inflammatory cytokines such as interleukin-1 beta and tumour necrosis factor alpha activate endothelial cells changing their resting phenotype to become pro-adhesive, pro-thrombotic and pro-atherogenic. Phage display in vivo biopann...

journal_title：FEBS letters

authors： Houston P,Goodman J,Lewis A,Campbell CJ,Braddock M

更新日期：2001-03-09 00:00:00

abstract：:Molecular dynamics analyses were performed to examine conformational changes in the C-domain of calmodulin and the N-domain of troponin C induced by binding of Ca(2+) ions. Analyses of conformational changes in calmodulin and troponin C indicated that the shortening of the distance between Ca(2+) ions and Ca(2+) bindi...

journal_title：FEBS letters

authors： Goto K,Toyama A,Takeuchi H,Takayama K,Saito T,Iwamoto M,Yeh JZ,Narahashi T

更新日期：2004-03-12 00:00:00

abstract：:Phosphorylation of the 20 kDa myosin light chain from smooth muscle by five different kinases was investigated. Three of the kinases (myosin light chain kinase, phosphorylase kinase, and cAMP-dependent protein kinase) phosphorylate serine residues, the fourth (casein-kinase-2) mainly threonine, and the fifth (glycogen...

journal_title：FEBS letters

authors： Singh TJ,Akatsuka A,Huang KP

更新日期：1983-08-08 00:00:00

abstract：:The nef gene of human and simian immunodeficiency virus is a key factor in acquired immunodeficiency syndrome pathogenesis and virus replication. Several Nef-induced phenomena, including the down-regulation of CD4 molecule, have been previously reported. In this study, we have identified and cloned a novel cellular pr...

journal_title：FEBS letters

authors： Fukushi M,Dixon J,Kimura T,Tsurutani N,Dixon MJ,Yamamoto N

更新日期：1999-01-08 00:00:00

abstract：:The maturation of Borna disease virus (BDV) glycoprotein GP was studied in regard to intracellular compartmentalization, compartmentalization signal-domains, proteolytic processing, and packaging into virus particles. Our data show that BDV-GP is (i) predominantly located in the endoplasmic reticulum (ER), (ii) partia...

journal_title：FEBS letters

authors： Eickmann M,Kiermayer S,Kraus I,Gössl M,Richt JA,Garten W

更新日期：2005-08-29 00:00:00

abstract：:Several myeloid precursors of antibacterial peptides have recently been shown to share homologous pre- and pro-regions. Taking advantage of this homology, a novel cDNA was cloned from pig bone marrow RNA. This encodes a 166-residue polypeptide with highly conserved pre- (29 residues) and pro- (101 residues) sequences,...

journal_title：FEBS letters

authors： Storici P,Scocchi M,Tossi A,Gennaro R,Zanetti M

更新日期：1994-01-17 00:00:00

abstract：:Screening of hemorphins in extracts of rat lung, brain, heart and spleen was carried out. The threshold for detection of hemorphins was 0.01 nmol for spleen and 0.05 nmol for other tissues. Both the content and the composition of hemorphins differed significantly in the tissues analyzed. Heart and lung extracts were r...

journal_title：FEBS letters

authors： Yatskin ON,Philippova MM,Blishchenko EYu,Karelin AA,Ivanov VT

更新日期：1998-05-29 00:00:00