Cloning and sequence analysis of a cDNA encoding rice glutaredoxin.

abstract：:Prostaglandin F2alpha, platelet-derived growth factor (PDGF) and calcium ionophore A23187 stimulated the rapid (within 25 s) generation of polyunsaturated 1,2-diacylglycerol (DAG) species, in particular 18:0/20:3n-9, 18:0/20:4n-6 and 18:0/20:5n-3, in Swiss 3T3 fibroblasts. This was followed by a second sustained phase...

journal_title：FEBS letters

authors： Pettitt TR,Wakelam MJ

更新日期：1998-05-15 00:00:00

abstract：:PMA-induced leukotriene C4 synthase (LTC4S) phosphorylation was investigated over a period of 8 h in a monocytic cell line (THP-1). The level of LTC4S phosphorylation was increased 3-5 fold over a 4 h period decreasing to basal levels after 8 h. This phosphorylation event was found to be specific to THP-1 cells as the...

journal_title：FEBS letters

authors： Gupta N,Nicholson DW,Ford-Hutchinson AW

更新日期：1999-04-16 00:00:00

abstract：:D-Aspartate increases human chorionic gonadotropin-induced testosterone production in purified rat Leydig cells. L-Aspartate, D-,L-glutamate or D-,L-asparagine could not substitute for D-aspartate and this effect was independent of glutamate receptor activation. Testosterone production was enhanced only in cells cultu...

journal_title：FEBS letters

authors： Nagata Y,Homma H,Lee JA,Imai K

更新日期：1999-02-12 00:00:00

abstract：:In the present study we have examined the levels and phosphorylation state of the insulin receptor and insulin receptor substrate 1 (IRS-1) as well as the association between IRS-1 and phosphatidylinositol 3-kinase (PI 3-kinase) in the liver and muscle of rats treated with glucagon. There was a decrease in the insulin...

journal_title：FEBS letters

authors： Saad MJ,Hartmann LG,de Carvalho DS,Galoro CA,Brenelli SL,Carvalho CR

更新日期：1995-08-14 00:00:00

abstract：:The eye lens protein and chaperonin, alpha-crystallin, was studied by differential scanning microcalorimetry, spectroscopy and size exclusion chromatography. The thermal transition of alpha-crystallin proceeds at Ttrs = 59.8 +/- 0.6 degrees C with an enthalpy change of delta H = 336 +/- 9 kJ per mol subunit. Disagreem...

journal_title：FEBS letters

authors： Gesierich U,Pfeil W

更新日期：1996-09-16 00:00:00

abstract：:While the majority of studies have focused on the biological roles of heparin-binding proteins, relatively little is known about their key residues and structural elements responsible for heparin interaction. In this study, we employed the IgG-binding domain B1 of Streptococcal protein G as a miniature scaffold to inv...

journal_title：FEBS letters

authors： Cheng YY,Cheng CS,Lee TR,Chang WS,Lyu PC

更新日期：2016-09-01 00:00:00

abstract：:The structure of rabbit liver Hsp90 was reevaluated by limited trypsinolysis, N-terminal sequencing and determination of the site that is phosphorylated by casein kinase II. Limited proteolysis results in formation of four groups of large peptides with M(r) in the range of 26-41 kDa. Peptides with M(r) 39-41 kDa were ...

journal_title：FEBS letters

authors： Bogatcheva NV,Ma Y,Urosev D,Gusev NB

更新日期：1999-09-03 00:00:00

abstract：:ROPs constitute a family of plant-specific, RHO-like small GTPases that serve as molecular switches in a wide range of signaling pathways. The activities of ROPs are regulated by guanine nucleotide exchange factors (GEFs). ROP11, a member of the ROP GTPase family in Arabidopsis, is a negative regulator of multiple ABA...

journal_title：FEBS letters

authors： Li Z,Liu D

更新日期：2012-05-07 00:00:00

abstract：:Mitogen activated protein kinase in extracts of U-937 macrophage-like cells was stimulated by LDL and oxLDL. A maximum value (161% of the basal phosphotransferase activity) was obtained after 6 min exposure to oxidized LDL (27 microgram/ml) using APRTPGGRR peptide substrate. The activatory effect was more pronounced (...

journal_title：FEBS letters

authors： Deigner HP,Claus R

更新日期：1996-05-06 00:00:00

abstract：:The 5 alpha-reductase activities in human prostatic nuclei and microsomes were compared. The activities in both subcellular fractions were identical with respect to pH dependence, heat inactivation and Michaelis constants for NADPH and testosterone. Subcellular distribution studies using DNA and nicotinamide mononucle...

journal_title：FEBS letters

authors： Houston B,Chisholm GD,Habib FK

更新日期：1985-06-17 00:00:00

abstract：:Here we report that splice blocking morpholinos (Sb MO) against zebrafish sox31 elicit developmental arrest, likely through creating a series of dominant negative splicing variants. Embryos injected with the Sb MO develop normally before the Mid-Blastula Transition (MBT); however, they do not initiate epiboly. Microar...

journal_title：FEBS letters

authors： Hu SN,Yu H,Zhang YB,Wu ZL,Yan YC,Li YX,Li YY,Li YP

更新日期：2012-02-03 00:00:00

abstract：:Previous data have suggested an involvement of MDR/PGP-like ABC transporters in transport of the plant hormone auxin and, recently, AtPGP1 has been demonstrated to catalyze the primary active export of auxin. Here we show that related isoform AtPGP4 is expressed predominantly during early root development. AtPGP4 loss...

journal_title：FEBS letters

authors： Santelia D,Vincenzetti V,Azzarello E,Bovet L,Fukao Y,Düchtig P,Mancuso S,Martinoia E,Geisler M

更新日期：2005-10-10 00:00:00

abstract：:The pl6INK4a tumor suppressor negatively regulates progression through the G1 phase of the mammalian cell cycle. To mimic the downmodulation of p16INK4a commonly seen in cancer, we designed and characterized a hammerhead ribozyme against exon E1alpha of the murine pl6INK4a transcript. Stable expression of the ribozyme...

journal_title：FEBS letters

authors： Nylandsted J,Rohde M,Bartek J,Strauss M

更新日期：1998-09-25 00:00:00

abstract：:The high affinity (low Km) cyclic GMP phosphodiesterase (PDE) is activated by GTP, while the cyclic AMP PDE is not. GTP and its hydrolysis-resistant analogue, guanylylimidodiphosphate (GppNHp), display a half-maximal stimulating effect at almost the same concentration (5 X 10(-6) M). The GTP stimulating effect is not ...

journal_title：FEBS letters

authors： Spina A,Chiosi E,Paolisso G,Laurenza A,Illiano G

更新日期：1983-07-04 00:00:00

abstract：:Tau proteins aggregate into different neuronal inclusions in several neurodegenerative disorders. In Alzheimer's disease (AD), hyperphosphorylated Tau from paired helical filaments (PHF) of neurofibrillary tangles, named PHF-Tau, have an electrophoretic profile with four main bands (Tau 55, 64, 69, 74 kDa). In Pick's ...

journal_title：FEBS letters

authors： Sergeant N,David JP,Lefranc D,Vermersch P,Wattez A,Delacourte A

更新日期：1997-08-04 00:00:00

abstract：:Plastoquinone-9 (PQ-9)-depleted PSII reaction center core complex, consisting of CP47/D1/D2/Cytb-559/I, was isolated from spinach PSII particles. PQ-9, lipids and several proteins were extracted from the original PSII particles and separated by several steps of chromatography to be reconstituted into the isolated comp...

journal_title：FEBS letters

authors： Kitamura K,Ozawa S,Shiina T,Toyoshima Y

更新日期：1994-10-31 00:00:00

abstract：:An efficient sensing of danger and a rapid activation of the immune system are crucial for the survival of plants. Conserved pathogen/microbe-associated molecular patterns (PAMPs/MAMPs) and endogenous molecular patterns, which are present only when the tissue is infected or damaged (damage-associated molecular pattern...

journal_title：FEBS letters

authors： De Lorenzo G,Brutus A,Savatin DV,Sicilia F,Cervone F

更新日期：2011-06-06 00:00:00

abstract：:Intravital multiphoton imaging allows visualization of infections and pathogenic mechanisms within intact organs in their physiological context. Today, most organs of mice and rats are applicable to in vivo or ex vivo imaging, opening completely new avenues for many researchers. Advances in fluorescent labeling of pat...

journal_title：FEBS letters

authors： Stolp B,Melican K

更新日期：2016-07-01 00:00:00

abstract：:A strain of yeast, Hansenula mrakii, secretes a toxin that kills sensitive yeasts, such as Saccharomyces cerevisiae Monoclonal antibodies raised against the toxin had both binding and neutralizing activities. The toxin in culture media was isolated by an affinity column of monoclonal antibody. The toxin is a basic pol...

journal_title：FEBS letters

authors： Yamamoto T,Imai M,Tachibana K,Mayumi M

更新日期：1986-01-20 00:00:00

abstract：:Three proteins of the mitochondrial inner membrane are known that are essential for the viability of yeast and seem to be involved in import of preproteins; the integral membrane proteins MIM17 and MIM23 and the peripheral membrane protein MIM44, MIM17 and MIM23 are homologous to each other in their hydrophobic domain...

journal_title：FEBS letters

authors： Kübrich M,Keil P,Rassow J,Dekker PJ,Blom J,Meijer M,Pfanner N

更新日期：1994-08-01 00:00:00

abstract：:Human natural killer (NK) cells possess an arsenal of receptors programmed to regulate the NK cell functions, once encountering a target cell. In general, the activating receptors mediate cytotoxicity when engaged by their tumor specific, stress induced, virally encoded, or rarely, self ligands. Whereas, the inhibitor...

journal_title：FEBS letters

authors： Stanietsky N,Mandelboim O

更新日期：2010-12-15 00:00:00

abstract：:Ankyrin repeat domain 17 (Ankrd17) encodes an ubiquitously expressed protein with two clusters of ankyrin repeats. We have used gene targeting strategy to ablate the Ankrd17 gene in mouse. The Ankrd17-deficient mice died between embryonic day (E) 10.5 and E11.5 due to cardiovascular defects. Serious hemorrhages were d...

journal_title：FEBS letters

authors： Hou SC,Chan LW,Chou YC,Su CY,Chen X,Shih YL,Tsai PC,Shen CK,Yan YT

更新日期：2009-09-03 00:00:00

abstract：:The AMP-activated protein kinase (AMPK) is the central component of a protein kinase cascade that plays a major role in energy sensing. AMPK is activated pharmacologically by 5-amino-4-imidazolecarboxamide (AICA) riboside monophosphate (ZMP), which mimics the effects of AMP on the AMPK cascade. Here we show that uptak...

journal_title：FEBS letters

authors： Fryer LG,Parbu-Patel A,Carling D

更新日期：2002-11-06 00:00:00

abstract：:UBR5 is an E3 ubiquitin ligase involved in distinct processes such as transcriptional regulation and development. UBR5 is highly upregulated in embryonic stem cells (ESCs), whereas its expression decreases with differentiation, suggesting a role for UBR5 in ESC function. However, little is known about how UBR5 regulat...

journal_title：FEBS letters

authors： Saez I,Gerbracht JV,Koyuncu S,Lee HJ,Horn M,Kroef V,Denzel MS,Dieterich C,Gehring NH,Vilchez D

更新日期：2020-01-01 00:00:00

abstract：:In unicellular species codon usage is determined by mutational biases and natural selection. Among prokaryotes, the influence of these factors is different if the genome is skewed towards AT or GC, since in AT-rich organisms translational selection is absent. On the other hand, in AT-rich unicellular eukaryotes the tw...

journal_title：FEBS letters

authors： Naya H,Romero H,Carels N,Zavala A,Musto H

更新日期：2001-07-20 00:00:00

abstract：:A method was established for measuring molecule numbers of three different myosin heavy chain (MHC) mRNA isoforms in total RNA preparations. The quantification was based on a combination of primer-directed reverse transcriptase and polymerase chain reactions with 5'-digoxigenin-labeled oligonucleotides, using external...

journal_title：FEBS letters

authors： Peuker H,Pette D

更新日期：1993-03-08 00:00:00

abstract：:The gene trap methodology is a powerful tool to characterize novel genes and analyze their importance in biological phenomena. It is based on the use of mouse embryonic stem cells and reporter vectors designed to randomly integrate into the genome, tagging an insertion site and generating a mutation. Theoretically, al...

journal_title：FEBS letters

authors： Cecconi F,Meyer BI

更新日期：2000-08-25 00:00:00

abstract：:Three mutant polypeptides of the type I 37-residue winter flounder 'antifreeze' protein have been synthesized. All four threonine residues in the native peptide were been mutated to serine, valine and glycine respectively and two additional salt bridges were incorporated into the sequences in order to improve aqueous ...

journal_title：FEBS letters

authors： Haymet AD,Ward LG,Harding MM,Knight CA

更新日期：1998-07-03 00:00:00

abstract：:Human respiratory syncytial virus (HRSV) is the leading cause of lower respiratory tract disease in infants. The HRSV small hydrophobic (SH) protein plays an important role in HRSV pathogenesis, although its mode of action is unclear. Analysis of the ability of SH protein to induce membrane permeability and form homo-...

journal_title：FEBS letters

authors： Carter SD,Dent KC,Atkins E,Foster TL,Verow M,Gorny P,Harris M,Hiscox JA,Ranson NA,Griffin S,Barr JN

更新日期：2010-07-02 00:00:00

abstract：:The calcitonin receptor (CTR) gene in human osteoclasts formed in a human bone marrow cell culture system was examined by reverse transcription-polymerase chain reaction (RT-PCR). The RT-PCR results indicated that the 5'-untranslated region (5'UTR) was different between CTR mRNAs in human osteoclasts and in a mammary ...

journal_title：FEBS letters

authors： Nishikawa T,Ishikawa H,Yamamoto S,Koshihara Y

更新日期：1999-09-24 00:00:00