Identification and expression-profiling of Xenopus tropicalis miRNAs including plant miRNA-like RNAs at metamorphosis.

abstract：:Isolated mitotic chromosomes are able to form complexes with phosphatidylcholine liposomes in the presence and absence of Ca2+ ions, in the latter case in the presence of polyamines. Interactions with chromosomes stimulates liposome fusion. The fusion is promoted by condensed and EDTA-decondensed chromosomes. ...

journal_title：FEBS letters

authors： Balakireva LA,Belyaev ND,Budker VG,Kiseleva EV

更新日期：1991-06-24 00:00:00

abstract：:ATP synthesis rate, measured as the variation in external Pi concentration, varied as a linear function of either delta mu H+ or delta Gpin, in such a manner that the delta Gpin/delta mu H+ ratio increased while VATP increased. We also observed a linear dependence of the flux control coefficient of the Pi carrier on...

journal_title：FEBS letters

authors： Beauvoit B,Rigoulet M,Guerin B

更新日期：1989-02-27 00:00:00

abstract：:The Kv3.1 channel plays a crucial role in regulating the high-frequency firing properties of neurons. Here, we determined whether Src regulates the subcellular distributions of the Kv3.1b channel. Co-expression of active Src induced a dramatic redistribution of Kv3.1b to the endoplasmic reticulum. Furthermore, co-expr...

journal_title：FEBS letters

authors： Bae SH,Kim DH,Shin SK,Choi JS,Park KS

更新日期：2014-01-03 00:00:00

abstract：:We show that the calcium-mimic dye, Stains-all, is a convenient probe to study the structural features of the individual calcium-binding sites of calmodulin (CaM) and related calcium-binding proteins (CaBP). These peptides bind the dye in their calcium-binding sites, and induce a circular dichroism (CD) band in the bo...

journal_title：FEBS letters

authors： Sharma Y,Gopalakrishna A,Balasubramanian D,Fairwell T,Krishna G

更新日期：1993-07-12 00:00:00

abstract：:Spermidine N1-acetyltransferase in an extract from phytohemagglutinin-stimulated bovine lymphocytes was inactivated by preincubation with alkaline phosphatase. Inactivation of the acetylase with the phosphatase was totally inhibited by addition of pyrophosphate. These results suggest that spermidine N1-acetyltransfera...

journal_title：FEBS letters

authors： Matsui I,Otani S,Kamei M,Morisawa S

更新日期：1982-12-13 00:00:00

abstract：:The effect of cAMP on the transcriptional activity of the HIV-1 long terminal repeat/enhancer was investigated and compared to the effect of cAMP on virus replication. In culture cAMP repressed virus replication in vivo using different cell types. Transient transfection studies with HIV-1 enhancer-derived luciferase r...

journal_title：FEBS letters

authors： Banas B,Eberle J,Banas B,Schlöndorff D,Luckow B

更新日期：2001-12-07 00:00:00

abstract：:Apolipoprotein E- (ApoE-) rich high-density lipoprotein (HDL), which was prepared from the bound fraction of normolipemic volunteers on heparin-Sepharose and from a hyperalphalipoproteinemic patient, potently inhibited aggregation of human platelets in a dose-dependent fashion. Dimyristoyl phosphatidylcholine liposome...

journal_title：FEBS letters

authors： Higashihara M,Kinoshita M,Teramoto T,Kume S,Kurokawa K

更新日期：1991-04-22 00:00:00

abstract：:Three of four natural compounds, which are caffeic acid, eupatilin and 4'-demethyleupatilin, isolated from Chinese plant, Artemisia rubripes Nakai selectively inhibited 5-lipoxygenase of cultured mastocytoma cells. Half-inhibition doses (ID50) for caffeic acid, eupatilin and 4'-demethyleupatilin were 3.7, 14 and 18 X ...

journal_title：FEBS letters

authors： Koshihara Y,Neichi T,Murota S,Lao A,Fujimoto Y,Tatsuno T

更新日期：1983-07-11 00:00:00

abstract：:A unique Cu,Zn-SOD was found and isolated from plaice Paralichthys olivaceus skin. Surprisingly, the properties of purified fish skin SOD were very different from those of SOD from other sources reported so far. The purified SOD was composed of four same subunits of 16 kDa and the molecular weight of the native SOD wa...

journal_title：FEBS letters

authors： Nakano T,Sato M,Takeuchi M

更新日期：1995-02-27 00:00:00

abstract：:Creatine kinases (CK) play a prominent role in cell energy distribution through an energy shuttle between mitochondria and other organelles. Human brain CK was cloned and overexpressed in COS-7 cells. We then deleted His-65 and/or Pro-66 situated near the center of a flexible loop as shown by X-ray crystallography on ...

journal_title：FEBS letters

authors： Mourad-Terzian T,Steghens JP,Min KL,Collombel C,Bozon D

更新日期：2000-06-09 00:00:00

abstract：:The sequence of seminalplasmin, a basic antimicrobial and transcription-inhibitory protein from bovine seminal plasma, has been determined using an automated sequenator. This sequence is slightly different from that reported earlier by Theil and Scheit [(1983) EMBO J. 2, 1159-1163] and identical with that of caltrin, ...

journal_title：FEBS letters

authors： Sitaram N,Kumari VK,Bhargava PM

更新日期：1986-06-09 00:00:00

abstract：:Sufficient highly purified native pea cytosolic ascorbate peroxidase was obtained to characterize some of its kinetic and spectral properties. Its rate constant for compound I formation from reaction with H2O2 is 4.O x 10(7) M-1 s-1, somewhat faster than is typical for peroxidases. Compound I has the typical optical s...

journal_title：FEBS letters

authors： Marquez LA,Quitoriano M,Zilinskas BA,Dunford HB

更新日期：1996-07-01 00:00:00

abstract：:Inducible heat shock protein 70 (Hsp70) is one of the most important HSPs for maintenance of cell integrity during normal cellular growth as well as pathophysiological conditions. Tumor necrosis factor receptor-associated factor 6 (TRAF6) is a crucial signaling transducer that regulates a diverse array of physiologica...

journal_title：FEBS letters

authors： Chen H,Wu Y,Zhang Y,Jin L,Luo L,Xue B,Lu C,Zhang X,Yin Z

更新日期：2006-05-29 00:00:00

abstract：:Filamin plays an important role in actin cytoskeleton organization, membrane stabilization, and anchoring of transmembrane proteins. Using short interfering RNA (siRNA) to selectively target the filamin A gene and silence its expression, we studied the role of filamin A in G protein coupled receptor (GPCR) signaling. ...

journal_title：FEBS letters

authors： Huang C,Wu Z,Hujer KM,Miller RT

更新日期：2006-03-20 00:00:00

abstract：:Genome maintenance and integrity requires continuous alterations of the compaction state of the chromatin structure. Chromatin remodelers, among others the INO80 complex, help organize chromatin by repositioning, reshaping, or evicting nucleosomes. We report on INO80 nucleosome remodeling, assayed by single-molecule F...

journal_title：FEBS letters

authors： Schwarz M,Schall K,Kallis E,Eustermann S,Guariento M,Moldt M,Hopfner KP,Michaelis J

更新日期：2018-02-01 00:00:00

abstract：:In contrast to the preponderance of affected males in families with X-linked mental retardation, Rett syndrome (RTT) is a neurological disorder occurring almost exclusively in females. The near complete absence of affected males in RTT families has been explained by the lethal effect of an X-linked gene mutation in he...

journal_title：FEBS letters

authors： Orrico A,Lam C,Galli L,Dotti MT,Hayek G,Tong SF,Poon PM,Zappella M,Federico A,Sorrentino V

更新日期：2000-09-22 00:00:00

abstract：:A 1000-bp fragment of Enterococcus hirae genomic DNA was amplified by the polymerase chain reaction method, using the oligonucleotide primers designed from amino acid sequences of both amino-terminal and a tryptic fragment of the Na(+)-ATPase alpha-subunit in this organism. DNA sequencing of this product revealed that...

journal_title：FEBS letters

authors： Kakinuma Y,Igarashi K,Konishi K,Yamato I

更新日期：1991-11-04 00:00:00

abstract：:The reaction of alpha-chymotrypsin with AcTyr-OEt and with AcTrp-OEt at pH 7.0 and 7.8 was studied over a wide range of substrate concentrations. The reaction with AcTyr-OEt at pH 7.8 was shown to be non-hyperbolic using a variety of criteria whereas those at pH 7.0 with the same substrate and at both pH values with A...

journal_title：FEBS letters

authors： Garrell J,Cuchillo CM

更新日期：1985-10-14 00:00:00

abstract：:Neuronal activity is often associated with changes in gene expression. By a two-dimensional cDNA-display system, restriction landmark cDNA scanning, we identified a novel gene whose expression in the hippocampus was up-regulated by kainate stimulation. The mRNA expression was detected only in brain and up-regulated by...

journal_title：FEBS letters

authors： Nakayama T,Yaoi T,Yasui M,Kuwajima G

更新日期：1998-05-22 00:00:00

abstract：:The effect of expression of c-fos gene on proteoglycan synthesis, one of the important markers of cartilage metabolism, was examined by introducing the c-fos DNA into HCS 2/8 chondrocytes. The [35S]sulfate incorporation into proteoglycan was decreased in the c-fos transfectants expressing exogenous c-fos mRNA, when co...

journal_title：FEBS letters

authors： Tsuji M,Funahashi S,Takigawa M,Seiki M,Fujii K,Yoshida T

更新日期：1996-03-04 00:00:00

abstract：:The fat mouse strain exhibits a late-onset obesity syndrome associated with a mutation in the gene encoding carboxypeptidase E (CPE). Since CPE plays a central role in the biosynthesis of a number of regulatory peptides, including gastrin, we examined the biogenesis and processing of progastrin in fat/fat mice by meas...

journal_title：FEBS letters

authors： Lacourse KA,Friis-Hansen L,Rehfeld JF,Samuelson LC

更新日期：1997-10-13 00:00:00

abstract：:Anandamide loses its cannabimimetic activities upon hydrolysis to arachidonic acid and ethanolamine. So far the anandamide hydrolyzing activity widely distributed in mammalian organs has been attributed exclusively to an enzyme referred to as anandamide amidohydrolase with an optimum pH around 9. We found another enzy...

journal_title：FEBS letters

authors： Ueda N,Yamanaka K,Terasawa Y,Yamamoto S

更新日期：1999-07-09 00:00:00

abstract：:The p53 tumor suppressor coordinates a multitude of cellular and organismal processes and exerts its activities mainly by activation of gene transcription. Here we describe the transcriptional activation of ectodysplasin A2 receptor (EDA2R) by p53 in a variety of cell types and tissues. We demonstrate that treatment o...

journal_title：FEBS letters

authors： Brosh R,Sarig R,Natan EB,Molchadsky A,Madar S,Bornstein C,Buganim Y,Shapira T,Goldfinger N,Paus R,Rotter V

更新日期：2010-06-03 00:00:00

abstract：:The GTPase RhoA is essential for the development of pre-T cells in the thymus. To investigate the mechanisms used by RhoA to control thymocyte development we have used Affymetrix gene profiling to identify RhoA regulated genes in T cell progenitors. The data show that RhoA plays a specific and essential role in pre-T ...

journal_title：FEBS letters

authors： Mullin M,Lightfoot K,Clarke R,Miller M,Lahesmaa R,Cantrell D

更新日期：2007-09-04 00:00:00

abstract：:Tau is reversibly hyperphosphorylated in the mouse brain by starvation or cold water swimming. Here, we report tau phosphorylation in the hippocampus of normal mouse after ether anesthesia, known to trigger typical stress reactions. Robust phosphorylation of tau was observed immediately and 10min after ether vapor exp...

journal_title：FEBS letters

authors： Ikeda Y,Ishiguro K,Fujita SC

更新日期：2007-03-06 00:00:00

abstract：:Targeting of preproteins to mitochondria and their translocation across the outer membrane are mediated by the mitochondrial receptor complex. This protein complex contains the import receptors MOM19 and MOM72 and the general insertion pore GIP. All seven components of the receptor complex are synthesized in the cytos...

journal_title：FEBS letters

authors： Keil P,Pfanner N

更新日期：1993-04-26 00:00:00

abstract：:The complexity of the proteasome gene family in higher plants was investigated by identification and sequencing cDNA clones from the Arabidopsis thaliana database showing homologies to 20S proteasome subunits. We identified plant counterparts for each of the 14 proteasomal subunit subfamilies. Moreover, several of the...

journal_title：FEBS letters

authors： Parmentier Y,Bouchez D,Fleck J,Genschik P

更新日期：1997-10-27 00:00:00

abstract：:Human class Alpha glutathione transferase (GST) A1-1 has been subjected to site-directed mutagenesis of a Tyr residue conserved in all classes of cytosolic GSTs. The change of Tyr8----Phe lowers the specific activities with three substrates to 2-8% of the values for the wild-type enzyme. The changes in the kinetic par...

journal_title：FEBS letters

authors： Stenberg G,Board PG,Mannervik B

更新日期：1991-11-18 00:00:00

abstract：:Casein kinase Ialpha (CKIalpha) belongs to a family of serine/threonine protein kinases involved in membrane trafficking, RNA processing, mitotic spindle formation and cell cycle progression. In this report, we identified several CKIalpha interacting proteins including RCC1, high mobility group proteins 1 and 2 (HMG1,...

journal_title：FEBS letters

authors： Dubois T,Howell S,Zemlickova E,Aitken A

更新日期：2002-04-24 00:00:00

abstract：:Light stimulation of rod cells in vertebrate eyes may cause Ca2+ release from the intracellular disks. Radiolabelled tracers show that light causes a small hyperpolarization of intact disk stacks and redistribution of the ions Ca2+ and Cl-. ...

journal_title：FEBS letters

authors： Hughes SM,Brand MD

更新日期：1985-03-25 00:00:00