Feedback regulation of Ras2 guanine nucleotide exchange factor (Ras2-GEF) activity of Cdc25p by Cdc25p phosphorylation in the yeast Saccharomyces cerevisiae.

abstract：:We reported the preparation of a novel trehalose derivative based on enzymatic oxidation of trehalose by water-soluble glucose-3-dehydrogenase (G3DH) from marine bacterium Halomonas sp. alpha-15 cells. The product of G3DH enzymatic conversion was 3,3'-diketotrehalose (3,3'dkT), a novel trehalose derivative of which bo...

journal_title：FEBS letters

authors： Sode K,Akaike E,Sugiura H,Tsugawa W

更新日期：2001-01-26 00:00:00

abstract：:The stoichiometric complex formed between human leukocyte elastase and a synthetic MeO-Suc-Ala-Ala-Pro-Val chloromethyl ketone inhibitor was co-crystallized and its X-ray structure determined, using Patterson search methods. Its structure has been crystallographically refined to a final R value of 0.145 (8.0 and 2.3 A...

journal_title：FEBS letters

authors： Wei AZ,Mayr I,Bode W

更新日期：1988-07-18 00:00:00

abstract：:The regulatory mechanism by which GATA-3 suppresses IFN-gamma gene expression was investigated. A reduction of GATA-3 using RNA interference technology enhanced, whereas overexpression of GATA-3 suppressed IFN-gamma mRNA expression. IL-4 expression was reciprocally affected by GATA-3. GATA-3-mediated down-regulation o...

journal_title：FEBS letters

authors： Kaminuma O,Kitamura F,Kitamura N,Miyagishi M,Taira K,Yamamoto K,Miura O,Miyatake S

更新日期：2004-07-16 00:00:00

abstract：:The cDNA of the human muscle type acylphosphatase was isolated and characterized. The mRNA presents a very long 5'-untranslated region, covering the first half of the molecule: 175 bases of this part were cloned and prediction of the possible secondary structure showed that a very stable stem-loop structure could be f...

journal_title：FEBS letters

authors： Fiaschi T,Marzocchini R,Raugei G,Veggi D,Chiarugi P,Ramponi G

更新日期：1997-11-03 00:00:00

abstract：:Interleukin-4 (IL-4) is a pleiotropic cytokine, which acts on both hematopoietic and non-hematopoietic cells, through different types of receptor complexes. In this study, we report that in human B cells, IL-4 caused rapid phosphorylation of Janus kinase (JAK) 1 and JAK3 tyrosine kinases. In keratinocytes, the hematop...

journal_title：FEBS letters

authors： Wery-Zennaro S,Letourneur M,David M,Bertoglio J,Pierre J

更新日期：1999-12-24 00:00:00

abstract：:Tertiary structure models of Interleukin-6 were constructed using a routine prediction method based on the X-ray crystal structures of granulocyte colony-stimulating factor (GCSF) and leukemia inhibitory factor (LIF). The models were evaluated with the aid of the sequence-structure compatibility (3D-1D) method program...

journal_title：FEBS letters

authors： Sumikawa H,Fukuhara K,Suzuki E,Matsuo Y,Nishikawa K

更新日期：1997-03-10 00:00:00

abstract：:The Golgi apparatus is known to modify and sort newly synthesized secretory proteins. However, fundamental mysteries remain about the structure, operation, and dynamics of this organelle. Important insights have emerged from studying the Golgi in yeasts. For example, yeasts have provided direct evidence for Golgi cist...

journal_title：FEBS letters

authors： Papanikou E,Glick BS

更新日期：2009-12-03 00:00:00

abstract：:The prohormone convertase PC2 is one of the major subtilisin/kexin-like enzymes responsible for the formation of small bioactive peptides in neural and endocrine cells. This convertase is unique among the members of the subtilisin/kexin-like mammalian serine proteinase family in that it undergoes zymogen processing of...

journal_title：FEBS letters

authors： Benjannet S,Mamarbachi AM,Hamelin J,Savaria D,Munzer JS,Chrétien M,Seidah NG

更新日期：1998-05-22 00:00:00

abstract：:The metabolite-modulated association of a fraction of hexokinase to mitochondria in brain is well documented, however, the involvement of other non-mitochondrial components in the binding of the hexokinase is controversial. Now we present evidence that the hexokinase binds both tubulin and microtubules in brain in vit...

journal_title：FEBS letters

authors： Wágner G,Kovács J,Löw P,Orosz F,Ovádi J

更新日期：2001-11-30 00:00:00

abstract：:A Trp-free alphaA-crystallin mutant (W9F) was prepared by site-directed mutation. This mutant appears to be identical to the wild-type in terms of conformation (secondary and tertiary structures). W9F was labeled with a sulfhydryl-specific fluorescent probe, 2-(4'-maleimidylanilino) naphthalene-6-sulfonate (MIANS), an...

journal_title：FEBS letters

authors： Sun TX,Akhtar NJ,Liang JJ

更新日期：1998-07-03 00:00:00

abstract：:The structure of the complex of Ras with the Ras-binding domain of its effector RalGDS (RGS-RBD), the first genuine Ras-effector complex, has been solved by X-ray crystallography. As with the Rap-RafRBD complex (Nasser et al., 1995), the interaction is via an inter-protein beta-sheet between the switch I region of Ras...

journal_title：FEBS letters

authors： Vetter IR,Linnemann T,Wohlgemuth S,Geyer M,Kalbitzer HR,Herrmann C,Wittinghofer A

更新日期：1999-05-21 00:00:00

abstract：:We report on the cDNA cloning and characterization of a novel short-chain isoprenyl diphosphate synthase from the aphid Myzus persicae. Of the three IPPS cDNAs we cloned, two yielded prenyltransferase activity following expression in Escherichia coli; these cDNAs encode identical proteins except for the presence, in o...

journal_title：FEBS letters

authors： Vandermoten S,Charloteaux B,Santini S,Sen SE,Béliveau C,Vandenbol M,Francis F,Brasseur R,Cusson M,Haubruge E

更新日期：2008-06-11 00:00:00

abstract：:The purinergic receptor P2X(7) is part of a complex signaling mechanism participating in a variety of physiological and pathological processes. Depending on the activation scheme, P2X(7) receptors in vivo are non-selective cation channels or form large pores that can mediate apoptotic cell death. Expression of P2X(7)R...

journal_title：FEBS letters

authors： Locovei S,Scemes E,Qiu F,Spray DC,Dahl G

更新日期：2007-02-06 00:00:00

abstract：:In bovine adrenocortical cells, the fatty acyl chains of the phospholipids have been identified in the membranes of the different cell compartments: plasma membranes, Golgi complex and coated vesicle membranes. An increase in the total number of unsaturation in the fatty acid is demonstrated in the coated vesicle memb...

journal_title：FEBS letters

authors： de Paillerets C,Bomsel M,Weintraub H,Pépin D,Alfsen A

更新日期：1987-07-13 00:00:00

abstract：:Factor VIIa is a vitamin K-dependent enzyme whose gamma-carboxyglutamic acid (Gla)-containing domain is important for calcium ion-dependent binding to the cofactor tissue factor and membrane surfaces. This domain contains 10 Gla residues, the individual roles and importance of which are not known. Comparisons with the...

journal_title：FEBS letters

authors： Persson E,Nielsen LS

更新日期：1996-05-06 00:00:00

abstract：:The L1CAM antibody A10-A3 efficiently reduces tumor growth in a nude mouse model. Here, we describe the crystal structure of the Fab fragment of A10-A3 determined at 2.0 angstrom resolution. The A10-A3 antibody H3 loop reveals a characteristic arrangement of exposed aromatic residues that may play an important role in...

journal_title：FEBS letters

authors： Wei CH,Lee ES,Jeon JY,Heo YS,Kim SJ,Jeon YH,Kim KH,Hong HJ,Ryu SE

更新日期：2011-01-03 00:00:00

abstract：:The COP9 signalosome (CSN) is a platform for protein communication in eukaryotic cells. It has an intrinsic metalloprotease that removes the ubiquitin (Ub)-like protein Nedd8 from cullins. CSN-mediated deneddylation regulates culling-RING Ub ligases (CRLs) and controls ubiquitination of proteins involved in DNA damage...

journal_title：FEBS letters

authors： Hannss R,Dubiel W

更新日期：2011-09-16 00:00:00

abstract：:Increases in choline containing metabolites have been associated with a number of disorders, including malignant cell growth. In this study, high resolution magic angle spinning (1)H nuclear magnetic resonance spectroscopy was employed to monitor metabolite changes during cell transfection, and an increase in phosphoc...

journal_title：FEBS letters

authors： Griffin JL,Mann CJ,Scott J,Shoulders CC,Nicholson JK

更新日期：2001-12-07 00:00:00

abstract：:We established robust, reliable protocols for 'Differential Display (DD),' an RNA fingerprinting method originally developed by Liang and Pardee [(1992) Science 257, 967-971] using RT-PCR with arbitrary primers. Our protocols are optimized so that reliable DD analysis can be performed on a fluorescent DNA sequencer to...

journal_title：FEBS letters

authors： Ito T,Kito K,Adati N,Mitsui Y,Hagiwara H,Sakaki Y

更新日期：1994-09-05 00:00:00

abstract：:C6 rat glioma cells persistently infected with subacute sclerosing panencephalitis virus (C6/SSPE) were treated with measles antiserum and purified anti-measles IgG. This stimulated phosphoinositide breakdown and an increase in inositol phosphates. In uninfected C6 cells, however, only fetal calf serum (FCS), but not ...

journal_title：FEBS letters

authors： Weinmann-Dorsch C,Koschel K

更新日期：1989-04-24 00:00:00

abstract：:Galectin-3, with a wide tissue distribution and marked developmental regulation, provides significant insights into the progression of various disease and developmental stages. Recognized by its specificity for galactose, a detailed characterization of its sugar binding ability has been investigated by isothermal titr...

journal_title：FEBS letters

authors： Bachhawat-Sikder K,Thomas CJ,Surolia A

更新日期：2001-06-29 00:00:00

abstract：:Cytochrome c oxidase has been isolated from beef heart mitochondria and labeled with the fluorochrome eosin-5-maleimide (EMA) after pretreatment with mersalyl. On SDS-polyacrylamide gels, EMA fluorescence and absorption occurred at a single band corresponding to subunit III. Since only Cys 115 of the two cysteinyl res...

journal_title：FEBS letters

authors： Müller M,Azzi A

更新日期：1985-05-06 00:00:00

abstract：:As a conserved non-coding RNA gene, transcripts of MALAT-1 localize predominately in the nucleus. However in G2/M cell cycle phase, MALAT-1 transcripts were surprisingly found to translocate from the nucleus into the cytoplasm. Investigation also found that in this process MALAT-1 interacts with an abundant nuclear fa...

journal_title：FEBS letters

authors： Yang F,Yi F,Han X,Du Q,Liang Z

更新日期：2013-10-01 00:00:00

abstract：:The synthesis of gangliosides GM3 and GD3 is carried out by the successive addition of sialic acid residues on lactosylceramide (LacCer) by the Golgi located sialyltransferases Sial-T1 and Sial-T2, respectively. CHO-K1 cells lack Sial-T2 and only express GM3. Here we show that the activity of Sial-T1 was near 2.5-fold...

journal_title：FEBS letters

authors： Spessott W,Crespo PM,Daniotti JL,Maccioni HJ

更新日期：2012-07-30 00:00:00

abstract：:In human brain the Abeta peptide is produced mainly by neurons and the overexpression of amyloid precursor protein (APP) that involves an increase in Abeta secretion, has been observed in some areas of the Alzheimer's disease patients brain. We have generated two stably transfected human neuroblastoma lines which over...

journal_title：FEBS letters

authors： Recuero M,Serrano E,Bullido MJ,Valdivieso F

更新日期：2004-07-16 00:00:00

abstract：:A novel type IV collagen-degrading metalloproteinase was purified from the conditioned media of a murine metastatic sarcoma cell line. The molecular weight of the purified enzyme was determined to be 100 kDa by SDS-PAGE, while 700 kDa by gel filtration suggesting that the enzyme has a multimer structure. This enzyme d...

journal_title：FEBS letters

authors： Kato Y,Ogawa K,Yamamoto S,Abe S,Kishi J,Hayakawa T

更新日期：1990-07-30 00:00:00

abstract：:Mutations of the cysteines (positions 668 and 714) were generated in the IF2 C domain of Bacillus stearothermophilus translation initiation factor IF2. The corresponding proteins were characterized functionally and structurally. Most (yet not all) amino acid replacements at both positions resulted in severe reduction ...

journal_title：FEBS letters

authors： Misselwitz R,Welfle K,Krafft C,Welfle H,Brandi L,Caserta E,Gualerzi CO

更新日期：1999-10-15 00:00:00

abstract：:Olfactory cilia contain cyclic nucleotide-gated and Ca2+-dependent Cl- conductances that underlie excitatory chemotransduction, and a Ca2+-dependent K+ (KCa) conductance, apparently involved in inhibitory transduction. Previous single-channel patch-clamp studies on olfactory cilia revealed four different KCas, with di...

journal_title：FEBS letters

authors： Castillo K,Bacigalupo J,Wolff D

更新日期：2005-03-14 00:00:00

abstract：:Acid extracts of rat pituitary neuro-intermediate lobes have been shown by ion-exchange chromatography and radio-immunoassay to contain predominantly the inactive derivatives of beta-endorphin, alpha, N-acetyl beta-endorphin 1-27 and alpha, N-acetyl beta-endorphin 1-26; the biologically active form, beta-endorphin 1-3...

journal_title：FEBS letters

authors： Ham J,Smyth DG

更新日期：1984-10-01 00:00:00

abstract：:The post-translational modification of histone proteins plays an important role in controlling cell fate by directing essentially all DNA-associated nuclear processes. Misregulation and mutation of histone modifying enzymes is a hallmark of tumorigenesis. However, how these different epigenetic modifications lead to t...

journal_title：FEBS letters

authors： Johnsen SA

更新日期：2012-06-04 00:00:00