Abstract:
:To investigate additional functions of the T cell adaptor, Src homology 2 (SH2) domain-containing leukocyte protein of 76 kD (SLP-76), we performed a yeast two-hybrid assay using the N-terminal region of SLP-76 fused with the kinase domain of Syk. By screening a human leukemia cDNA library, we identified the p85 subunit of phosphoinositide 3-kinase (PI3K) as one of the interacting molecules. Unlike the SH2 domain of Vav or Nck, tyrosine phosphorylation of SLP-76 at position 113 or 128 was sufficient for it to associate with the N-terminal SH2 of p85. Collectively, these data suggest that SLP-76 may play a role in PI3K signaling pathways.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Shim EK,Moon CS,Lee GY,Ha YJ,Chae SK,Lee JRdoi
10.1016/j.febslet.2004.07.090keywords:
subject
Has Abstractpub_date
2004-09-24 00:00:00pages
35-40issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579304010324journal_volume
575pub_type
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