Abstract:
:The octameric ring protein, Translin, demonstrates marked similarities to the family of helicase enzymes regarding its quaternary organization and dimerization of subunits. Here we show that the level of Translin closely parallels the proliferative state in various cell types. Expression is periodic during the cell cycle, with protein synthesis becoming maximal in the S and mitosis phases, consistent with a role in cell division. Moreover, induced overexpression of Translin was found to accelerate cell proliferation. Confocal microscopic analysis revealed that Translin is localized at the centrosomes at prophase and the mitotic spindle at metaphase, then translocating to the spindle midbodies during cytokinesis. This novel localization is attributable to specific interactions with microtubules of the mitotic spindles, and especially gamma-tubulin. The results suggest that Translin participates in processes ensuring the segregation of chromosomes and cytokinesis.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Ishida R,Okado H,Sato H,Shionoiri C,Aoki K,Kasai Mdoi
10.1016/s0014-5793(02)03095-8keywords:
subject
Has Abstractpub_date
2002-08-14 00:00:00pages
105-10issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579302030958journal_volume
525pub_type
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