Rapid disruption of intestinal barrier function by gliadin involves altered expression of apical junctional proteins.

abstract：:The activation of peroxisome proliferator-activated receptor gamma (PPARgamma) has been shown to inhibit the production and the effects of proinflammatory cytokines. Since interleukin-1beta (IL-1beta) directly mediates cartilage degradation in osteoarthritis, we investigated the capability of PPARgamma ligands to modu...

journal_title：FEBS letters

authors： Boyault S,Simonin MA,Bianchi A,Compe E,Liagre B,Mainard D,Bécuwe P,Dauça M,Netter P,Terlain B,Bordji K

更新日期：2001-07-13 00:00:00

abstract：:We have used a gene replacement strategy to delete the previously isolated gene [(1987) EMBO J. 6, 2825-2833] for the cytochrome c oxidase subunit I from Paracoccus denitrificans. The resulting mutant was still able to synthesize active cytochrome c oxidase. This led us to look for another locus which could completely...

journal_title：FEBS letters

authors： Raitio M,Pispa JM,Metso T,Saraste M

更新日期：1990-02-26 00:00:00

abstract：:Endothelin (ET-1) has been shown to exert vasoconstrictor activity in vivo and mobilize Ca2+ in vascular smooth muscle cells in culture. In this paper we show that the human skin fibroblast exhibits specific receptors to ET-1 and that activation of these receptors results in increased intracellular Ca2+ (Ca2+i) and ac...

journal_title：FEBS letters

authors： Gardner JP,Maher E,Aviv A

更新日期：1989-10-09 00:00:00

abstract：:The actin-depolymerising factor (ADF)/cofilin group of proteins are stimulus-responsive actin-severing proteins, members of which are regulated by reversible phosphorylation. The phosphorylation site on the maize ADF, ZmADF3, is Ser-6 but the kinase responsible is unknown [Smertenko et al., Plant J. 14 (1998) 187-193]...

journal_title：FEBS letters

authors： Allwood EG,Smertenko AP,Hussey PJ

更新日期：2001-06-15 00:00:00

abstract：:We have recently developed a method to produce native human proinsulin using a bacterial expression system. A proinsulin fusion protein was recovered from inclusion bodies and cleaved using cyanogen bromide. The released proinsulin polypeptide was S-sulfonated and purified by anion exchange chromatography. Following r...

journal_title：FEBS letters

authors： Cowley DJ,Mackin RB

更新日期：1997-02-03 00:00:00

abstract：:Jasmonic acid (JA) has been shown to be a signaling compound which elicits the production of secondary metabolites including phytoalexins in plants. It has been shown that the phytoalexin production is elicited by exogenously applied JA in rice leaves. We now show that this phytoalexin production by exogenously applie...

journal_title：FEBS letters

authors： Tamogami S,Rakwal R,Kodama O

更新日期：1997-07-21 00:00:00

abstract：:CpG dinucleotides are efficiently methylated in vertebrate genomes except in the CpG islands having a high C+G content. Methylated CpGs are the single most mutated dinucleotide. Sequences surrounding disease causing CpG mutation sites were analyzed from locus-specific mutation databases. Both tetra- and heptanucleotid...

journal_title：FEBS letters

authors： Ollila J,Lappalainen I,Vihinen M

更新日期：1996-11-04 00:00:00

abstract：:Lipid transfer proteins (LTPs) and elicitins are both able to load and transfer lipidic molecules and share some structural and functional properties. While elicitins are known as elicitors of plant defence mechanisms, the biological function of LTP is still an enigma. We show that a wheat LTP1 binds with high affinit...

journal_title：FEBS letters

authors： Buhot N,Douliez JP,Jacquemard A,Marion D,Tran V,Maume BF,Milat ML,Ponchet M,Mikès V,Kader JC,Blein JP

更新日期：2001-11-30 00:00:00

abstract：:Tumor necrosis factor alpha (TNF-α) is a prototypical proinflammatory cytokine that can elicit strong inflammation in macrophages by activating NF-κB. The underlying epigenetic mechanism is obscure. We show here that megakaryocytic leukemia 1 (MKL1) is an epigenetic mediator of TNF-α-induced proinflammatory transcript...

journal_title：FEBS letters

authors： Song M,Fang F,Dai X,Yu L,Fang M,Xu Y

更新日期：2017-03-01 00:00:00

abstract：:Analysis of fluorimetric equilibrium-binding isotherms of a proteolytic fragment of bacteriophage T4 gene 32 protein (g32P) lacking residues 1-9 shows that this region contains the site responsible for the function of the NH2-terminal 'B' domain (residues 1-21). The end codon of the frameshift mutant g32P-PR201 has be...

journal_title：FEBS letters

authors： Casas-Finet JR

更新日期：1989-06-05 00:00:00

abstract：:In the unfolded protein response (UPR), Ire1 activates Hac1 to coordinate the transcription of hundreds of genes to mitigate ER stress. Recent work in Caenorhabditis elegans suggests that oxidative stress inhibits this canonical Ire1 signalling pathway, activating instead an antioxidant stress response. We sought to d...

journal_title：FEBS letters

authors： Guerra-Moreno A,Ang J,Welsch H,Jochem M,Hanna J

更新日期：2019-05-01 00:00:00

abstract：:Purified plasma membrane vesicles isolated from multidrug-resistant human KB-V1 cells accumulate [3H]vinblastine in an energy-dependent manner. The accumulation of [3H]vinblastine in the presence of ATP is a saturable process. ATP can be replaced by other purine nucleotide triphosphates, of which GTP is the most effic...

journal_title：FEBS letters

authors： Lelong IH,Padmanabhan R,Lovelace E,Pastan I,Gottesman MM

更新日期：1992-06-15 00:00:00

abstract：:Histone deacetylase 6 (HDAC6) is a multi-substrate cytoplasmic enzyme that regulates many important biological processes. Recently, some reports have implicated HDAC6 in viral infection. However, nothing is known about its regulation in virus-infected cells. The data presented here for the first time demonstrate the c...

journal_title：FEBS letters

authors： Husain M,Harrod KS

更新日期：2009-08-06 00:00:00

abstract：:The folding of HIV gp41 into a 6-helix bundle drives virus-cell membrane fusion. To examine the structural relationship between the 6-helix bundle core domain and other regions of gp41, we expressed in Escherichia coli, the entire ectodomain of HIV-2(ST) gp41 as a soluble, trimeric maltose-binding protein (MBP)/gp41 c...

journal_title：FEBS letters

authors： Lay CS,Wilson KA,Kobe B,Kemp BE,Drummer HE,Poumbourios P

更新日期：2004-06-04 00:00:00

abstract：:WW domains harbor substrates containing proline-rich motifs, but the substrate specificity and binding mechanism remain elusive for those WW domains less amenable for structural studies, such as human WWP2 (hWWP2). Herein we have employed multiple techniques to investigate the second WW domain (WW2) in hWWP2. Our resu...

journal_title：FEBS letters

authors： Jiang J,Wang N,Jiang Y,Tan H,Zheng J,Chen G,Jia Z

更新日期：2015-07-08 00:00:00

abstract：:Although neurokinin A (NKA), a tachykinin peptide with sequence homology to substance P (SP), is a weak competitor of radiolabeled SP binding to the NK-1 receptor (NK-1R), more recent direct binding studies using radiolabeled NKA have demonstrated an unexpected high-affinity interaction with this receptor. To document...

journal_title：FEBS letters

authors： Bremer AA,Leeman SE,Boyd ND

更新日期：2000-12-01 00:00:00

abstract：:We previously showed that the post-translational cleavage of alphav subunit is essential for integrin-dependent signalling and cell adhesion. Here, we report that blocking alphav subunit cleavage by expression of alpha1-PDX, a convertase inhibitor, modified the capacity of cells to change shape, via a remodelling of t...

journal_title：FEBS letters

authors： Berthet V,Rigot V,Nejjari M,Marvaldi J,Luis J

更新日期：2004-01-16 00:00:00

abstract：:Hepatitis C virus (HCV) replication and pathogenesis involve both virus-encoded proteins and cellular factors. In our study, we showed that NS5B, the HCV RNA-dependent RNA polymerase, interacted with M2 type pyruvate kinase (M2PK) but not L type pyruvate kinase. We confirmed the interaction by GST pull down, coimmunop...

journal_title：FEBS letters

authors： Wu X,Zhou Y,Zhang K,Liu Q,Guo D

更新日期：2008-06-25 00:00:00

abstract：:Rabbit red blood cells of various ages were separated on Percoll gradients and the activities of two large cytosolic proteases were measured. Both the multicatalytic protease (MCP), assayed by hydrolysis of fluorigenic peptides, and the 26 S ubiquitin/ATP-stimulated protease, assayed by degradation of ubiquitin-lysozy...

journal_title：FEBS letters

authors： Di Cola D,Pratt G,Rechsteiner M

更新日期：1991-03-11 00:00:00

abstract：:Enhancement by thrombin of Ca2+-dependent 5HT secretion in the absence of added GTP decreases as the time between electropermeabilisation and addition of thrombin is increased. No decrease occurs if thrombin is added with GTP. Observation of apparent GTP-independent receptor/phospholipase C coupling may result from th...

journal_title：FEBS letters

authors： Knight DE,Scrutton MC

更新日期：1987-10-19 00:00:00

abstract：:The cytosolic lipid peroxidation product 4-hydroxynonenal (HNE) is rapidly metabolized in mitochondria isolated from rat kidney cortex. About 80% of HNE was degraded within 3 min of incubation. Main products of HNE which were identified in mitochondria were the hydroxynonenoic acid, the 1,4-dihydroxynonene and the glu...

journal_title：FEBS letters

authors： Ullrich O,Grune T,Henke W,Esterbauer H,Siems WG

更新日期：1994-09-19 00:00:00

abstract：:The tuf gene of eubacteria, encoding the EF-tu elongation factor, was duplicated early in the evolution of the taxon. Phylogenetic and genomic location analysis of 20 complete eubacterial genomes suggests that this ancient duplication has been differentially lost and maintained in eubacteria. ...

journal_title：FEBS letters

authors： Lathe WC 3rd,Bork P

更新日期：2001-08-03 00:00:00

abstract：:The contribution of hepatic glycogen to lipogenesis was studied in isolated, intact rat hepatocytes. To establish its importance as a substrate for lipogenesis, the glycogen of isolated hepatocytes was prelabelled with 14C from glucose. Evidence is presented that neither glucose nor glycogen constitute major sources o...

journal_title：FEBS letters

authors： Geelen MJ,Hindriks GA

更新日期：1984-09-17 00:00:00

abstract：:Parathyroid hormone (PTH) binds to its receptor (PTH 1 receptor, PTH1R) and activates multiple pathways. The PTH1R, a class b GPCR, contains consensus calmodulin-binding motifs. The PTH1R cytoplasmic tail interacts with calmodulin in a calcium-dependent manner via the basic 1-5-8-14 motif. Calcium-dependent calmodulin...

journal_title：FEBS letters

authors： Mahon MJ,Shimada M

更新日期：2005-01-31 00:00:00

abstract：:Modelling the amino acid sequence of NAD(+)-linked 15-hydroxyprostaglandin dehydrogenase into the three-dimensional structure of 3 alpha/20 beta-hydroxysteroid dehydrogenase shows that these two enzymes, as well as the NADP(+)-linked prostaglandin dehydrogenase (identical to carbonyl reductase) have similar conformati...

journal_title：FEBS letters

authors： Krook M,Ghosh D,Duax W,Jörnvall H

更新日期：1993-05-10 00:00:00

abstract：:Recently the identity of the peptidyl-prolyl cis-trans isomerase (PPIase), which accelerates the cis/trans isomerization of prolyl peptide bonds and cyclophilin, the binding protein for the immunosuppressive drug Cyclosporin A (CsA), was discovered. The PPIase catalysis toward the substrate Suc-Ala-Phe-Pro-Phe-pNA has...

journal_title：FEBS letters

authors： Hübner D,Drakenberg T,Forsén S,Fischer G

更新日期：1991-06-17 00:00:00

abstract：:The nucleotide sequences of the dnaQ genes from Salmonella typhimurium and Buchnera aphidicola, encoding the epsilon-subunit of the DNA polymerase III holoenzyme, have been determined. The Salmonella typhimurium dnaQ protein consists of 243 amino acid residues with a calculated molecular weight of 27224. The Buchnera ...

journal_title：FEBS letters

authors： Huang Y,Braithwaite DK,Ito J

更新日期：1997-01-02 00:00:00

abstract：:We report here the molecular cloning, nucleotide sequence, and predicted amino acid sequence of an alpha-subunit of the developmentally useful model, Artemia. The amino acid sequence shows divergence from that of mammals, birds, Torpedo, and Drosophila. However, regions in the putative ATP binding and transmembrane do...

journal_title：FEBS letters

authors： Baxter-Lowe LA,Guo JZ,Bergstrom EE,Hokin LE

更新日期：1989-10-23 00:00:00

abstract：:An electrometric technique was used to investigate electron transfer between spinach plastocyanin (Pc) and photooxidized primary electron donor P700 in photosystem I (PS I) complexes from the cyanobacterium Synechocystis sp. PCC 6803. In the presence of Pc, the fast unresolvable kinetic phase of membrane potential gen...

journal_title：FEBS letters

authors： Mamedov MD,Mamedova AA,Chamorovsky SK,Semenov AY

更新日期：2001-07-06 00:00:00

abstract：:Prodigiosin 25-C inhibited the accumulation of 3-(2,4-dinitroanilino)-3'-amino-N-methyldipropylamine and acridine orange in the acidic compartments of baby hamster kidney cells with little perturbation of cellular ATP levels. In rat liver lysosomes, prodigiosin 25-C inhibited the proton pump activity with an IC50 of a...

journal_title：FEBS letters

authors： Kataoka T,Muroi M,Ohkuma S,Waritani T,Magae J,Takatsuki A,Kondo S,Yamasaki M,Nagai K

更新日期：1995-02-06 00:00:00