Abstract:
:Abscisic acid (ABA) is a plant hormone involved in many developmental and physiological processes, but as yet, no ABA receptor has been identified. Flow cytometry of rice protoplasts and immunoblotting of purified plasma membranes (PMs) have been used to demonstrate that the monoclonal antibody JIM19 recognizes carbohydrate epitopes of cell surface glycoproteins. Using surface plasmon resonance technology specific binding of PMs to JIM19 was observed. Such interaction was antagonized significantly by ABA, but not by the biologically inactive ABA catabolite phaseic acid. These in vitro interactions were correlated with the biological activities of JIM19, ABA and phaseic acid on activation of the ABA-inducible Em promoter using two different transient reporter gene assays, beta-glucuronidase/luciferase and quantitative flow cytometry of Aequoria green fluorescent protein. Pre-treatment with JIM19 resulted in significant inhibition of ABA-inducible gene expression. Taken together, these data suggest that JIM19 interacts with a functional PM complex involved in ABA signalling.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Desikan R,Hagenbeek D,Neill SJ,Rock CDdoi
10.1016/s0014-5793(99)00972-2keywords:
subject
Has Abstractpub_date
1999-08-06 00:00:00pages
257-62issue
2eissn
0014-5793issn
1873-3468pii
S0014-5793(99)00972-2journal_volume
456pub_type
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