Abstract:
:Hexameric inorganic pyrophosphatase of Escherichia coli contains about 1 mol/mol of 'structural' pyrophosphate, which survives gel filtration and prolonged incubation with Mg2+, does not exchange with medium phosphate and pyrophosphate but is removed with 0.8 M perchloric acid. The site of pyrophosphate binding seems to be another than the active site. An additional 0.9 mol of enzyme-bound pyrophosphate is formed in the presence of phosphate and Mg2+ but this pyrophosphate is in fast equilibrium with medium phosphate and appears to be bound to the active site.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Shestakov AA,Baykov AA,Avaeva SMdoi
10.1016/0014-5793(90)80187-nsubject
Has Abstractpub_date
1990-03-26 00:00:00pages
194-6issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(90)80187-Njournal_volume
262pub_type
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