Abstract:
:At present, little is known about signal transduction mechanisms in schistosomes, which cause the disease of schistosomiasis. The mitogen-activated protein kinase (MAPK) signaling pathways, which are evolutionarily conserved from yeast to Homo sapiens, play key roles in multiple cellular processes. Here, we reconstructed the hypothetical MAPK signaling pathways in Schistosoma japonicum and compared the schistosome pathways with those of model eukaryote species. We identified 60 homologous components in the S. japonciumMAPK signaling pathways. Among these, 27 were predicted to be full-length sequences. Phylogenetic analysis of these proteins confirmed the evolutionary conservation of the MAPK signaling pathways. Remarkably, we identified S. japonicum homologues of GTP-binding protein beta and alpha-I subunits in the yeast mating pathway, which might be involved in the regulation of different life stages and female sexual maturation processes as well in schistosomes. In addition, several pathway member genes, including ERK, JNK, Sja-DSP, MRAS and RAS, were determined through quantitative PCR analysis to be expressed in a stage-specific manner, with ERK, JNK and their inhibitor Sja-DSP markedly upregulated in adult female schistosomes.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Wang L,Yang Z,Li Y,Yu F,Brindley PJ,McManus DP,Wei D,Han Z,Feng Z,Li Y,Hu Wdoi
10.1016/j.febslet.2006.05.055subject
Has Abstractpub_date
2006-06-26 00:00:00pages
3677-86issue
15eissn
0014-5793issn
1873-3468pii
S0014-5793(06)00668-5journal_volume
580pub_type
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