Abstract:
:Mammalian culture cells have the potential for periodicity, since high concentrations of serum can elicit the circadian expression of clock genes in rat-1 fibroblasts. However, the mechanism by which serum affects circadian gene expression remains unclear. In the present study, we incubated rat-1 cells with forskolin and successfully induced the rhythmic expression of Per1, Per2 and dbp. In the initial step of the circadian gene expression, a marked transient induction of Per1 was observed accompanied with CREB phosphorylation. Thus the present study strongly suggests that CREB activation through the cAMP/PKA pathway is involved in the generation of circadian rhythm in rat-1 cells
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Yagita K,Okamura Hdoi
10.1016/s0014-5793(99)01724-xkeywords:
subject
Has Abstractpub_date
2000-01-07 00:00:00pages
79-82issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(99)01724-Xjournal_volume
465pub_type
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