Linker histone H1FOO regulates the chromatin structure in mouse zygotes.

abstract：:Phosphorylcholine, phosphorylcreatine, lactate, glutathione, glycine and leucine were identified in living HeLa cells in the first study of this cell type by 1H spin echo NMR spectroscopy. The technique has the advantage that it is non-invasive, providing detailed structural information on individual species present i...

journal_title：FEBS letters

authors： Reglinski J,Smith WE,Suckling CJ,Al-Kabban M,Watson ID,Stewart MJ

更新日期：1987-04-20 00:00:00

abstract：:Bacillus subtilis heme A synthase is a membrane protein with 8 transmembrane segments. By using a two-step mutagenesis approach we have generated and selected a fully functional enzyme protein variant with a seven residue internal deletion. The biochemical properties of the shortened variant are similar to those of th...

journal_title：FEBS letters

authors： Lewin A,Hederstedt L

更新日期：2008-04-16 00:00:00

abstract：:Putrescine N-methyltransferase (PMT) catalyses S-adenosylmethionine (SAM)-dependent methylation of putrescine in tropane alkaloid biosynthesis. PMT presumably evolved from the ubiquitous spermidine synthase (SPDS). SPDS protein structure suggested that only few amino acid exchanges in the active site were necessary to...

journal_title：FEBS letters

authors： Biastoff S,Reinhardt N,Reva V,Brandt W,Dräger B

更新日期：2009-10-20 00:00:00

abstract：:Currently described substrates of the bacterial Tat protein transport system are directed for export by signal peptides containing a pair of invariant arginine residues. The signal peptide of the TtrB subunit of Salmonella enterica tetrathionate reductase contains a single arginine residue but is nevertheless able to ...

journal_title：FEBS letters

authors： Hinsley AP,Stanley NR,Palmer T,Berks BC

更新日期：2001-05-18 00:00:00

abstract：:Small-angle X-ray scattering was used to investigate a complex state of apocalmodulin induced by the binding of a Ca(2+)/calmodulin-dependent protein kinase IV calmodulin target site. Upon binding of the peptide, the molecular weight for apocalmodulin increased by 8.4%, which provides direct evidence for the formation...

journal_title：FEBS letters

authors： Izumi Y,Kuwamoto S,Jinbo Y,Yoshino H

更新日期：2001-04-20 00:00:00

abstract：:Boar spermadhesins are a group of seminal plasma, heparin-binding proteins which appear to be involved in sperm capacitation and gamete interaction. Using a proteolytic protection assay we have identified regions of AQN-1, AQN-3, PSP-I and AWN which remain attached to a heparin-Sepharose column following in-column dig...

journal_title：FEBS letters

authors： Calvete JJ,Dostàlova Z,Sanz L,Adermann K,Thole HH,Töpfer-Petersen E

更新日期：1996-02-05 00:00:00

abstract：:We have expressed in E. coli segments of the rod portion of rabbit skeletal fast muscle myosin and compared physical properties of two different species, LMM-30 and LMM-30C'. LMM-30 consists of 263 amino acids including the original C-terminus of myosin heavy chain. LMM-30C' is colinear with LMM-30, but is devoid of 1...

journal_title：FEBS letters

authors： Maeda K,Rösch A,Maéda Y,Kalbitzer HR,Wittinghofer A

更新日期：1991-04-09 00:00:00

abstract：:The kinetics of the tRNA cycle is in itself capable of keeping the translational error level almost unaffected by amino acid starvation. There is no need to assume any yet unknown mechanism or property. Kinetic analysis shows that the concentration of aminoacyl-tRNA can stay high even for large reductions in aminoacyl...

journal_title：FEBS letters

authors： Liljenström H

更新日期：1987-10-19 00:00:00

abstract：:Parathyroid hormone (PTH) binds to its receptor (PTH 1 receptor, PTH1R) and activates multiple pathways. The PTH1R, a class b GPCR, contains consensus calmodulin-binding motifs. The PTH1R cytoplasmic tail interacts with calmodulin in a calcium-dependent manner via the basic 1-5-8-14 motif. Calcium-dependent calmodulin...

journal_title：FEBS letters

authors： Mahon MJ,Shimada M

更新日期：2005-01-31 00:00:00

abstract：:A chloroperoxidase gene from Pseudomonas pyrrocinia was cloned into Escherichia coli using the cosmid vector pJB8. The gene coding for the chloroperoxidase could be localized to a 1.5 kb fragment of DNA which was subcloned into the high-copy-number plasmid pUC18. In one subclone increased halogenating activity could b...

journal_title：FEBS letters

authors： Wolfframm C,van Pée KH,Lingens F

更新日期：1988-10-10 00:00:00

abstract：:Those aerobic archaea whose genomes have been sequenced possess a single 4-gene operon that, by sequence comparisons with Bacteria and Eukarya, appears to encode the three component enzymes of a 2-oxoacid dehydrogenase multienzyme complex. However, no catalytic activity of any such complex has ever been detected in th...

journal_title：FEBS letters

authors： Heath C,Jeffries AC,Hough DW,Danson MJ

更新日期：2004-11-19 00:00:00

abstract：:To explore the structural basis required for the holoenzyme formation of cAMP-dependent protein kinase, we have prepared rabbit anti-peptide antibodies that can block the holoenzyme formation without affecting the catalytic activity of the enzyme. The antibodies were raised against a specific site in the catalytic (C)...

journal_title：FEBS letters

authors： Sahara S,Sato K,Kaise H,Mori K,Sato A,Aoto M,Tokmakov AA,Fukami Y

更新日期：1996-04-15 00:00:00

abstract：:Addition of cyanide to the CO complex of cytochrome oxidase reduces the apparent photosensitivity of the Fe-CO bond. This effect is not seen with azide, or when cyanide is added to ferromyoglobin-CO. It is proposed that cyanide binds to CuB, and restricts the passage of CO out of the protein. This restriction favors g...

journal_title：FEBS letters

authors： Hill BC

更新日期：1994-11-14 00:00:00

abstract：:Xanthine oxidase was decreased 2- to 10-fold in all examined rat hepatomas irrespective of the malignancy; growth rate and degrees of histological differentiation of the neoplasms. The affinity to substrate (KM=6-8 muM) and the pH optimum (8.0) of the liver and hepatoma enzymes were the same. The reprogramming of gene...

journal_title：FEBS letters

authors： Prajda N,Weber G

更新日期：1975-11-15 00:00:00

abstract：:By sequence analysis we show that the U104 domain found in the UNC104 subfamily of kinesins is a forkhead homology-associated domain (FHA). A combination of limited proteolysis, mass spectroscopy, and physicochemical analysis define this domain as a genuine autonomously folding domain. Our data show that the FHA domai...

journal_title：FEBS letters

authors： Westerholm-Parvinen A,Vernos I,Serrano L

更新日期：2000-12-15 00:00:00

abstract：:We have studied the action of an alpha-latrotoxin (alpha-LTX) complex of two polypeptides (LTX 130 kDa and low molecular weight protein (LMWP) 8 kDa) and the action of a venom fraction containing LTX with excess LMWP on calcium influx into synaptosomes and PC12 cells as well as on [14C]GABA release from synaptosomes. ...

journal_title：FEBS letters

authors： Grishin EV,Himmelreich NH,Pluzhnikov KA,Pozdnyakova NG,Storchak LG,Volkova TM,Woll PG

更新日期：1993-12-27 00:00:00

abstract：:Linkage between the high-capacity Ca2+-binding protein calsequestrin and the ryanodine receptor is proposed to be essential for proper Ca2+-release during skeletal muscle excitation-contraction coupling. However, no direct biochemical evidence exists showing a connection between these high-molecular-mass complexes in ...

journal_title：FEBS letters

authors： Murray BE,Ohlendieck K

更新日期：1998-06-16 00:00:00

abstract：:Rats were infused intravenously with a bolus of mevalonolactone or cholesterol-rich chylomicron and microsomes prepared from the livers at times up to 2 h after the infusion. Acyl-CoA cholesterol acyltransferase (ACAT) activity was measured in the microsomes under conditions that define the activity state of the enzym...

journal_title：FEBS letters

authors： Suckling KE,Stange EF,Dietschy JM

更新日期：1983-08-08 00:00:00

abstract：:Alix and its homologs are involved in various phenomena such as endosomal protein-sorting and adaptation to stress conditions. In this study, we found that development of Dictyostelium discoideum Alix (DdAlix) deletion mutant (alx-) cells was impaired in alkaline pH environments. The fruiting body formation efficiency...

journal_title：FEBS letters

authors： Ohkouchi S,Saito H,Aruga F,Maeda T,Shibata H,Maki M

更新日期：2005-03-14 00:00:00

abstract：:The unfolded protein response (UPR)--the endoplasmic reticulum stress response--is found in various pathologies including ischemia-reperfusion injury (IRI). However, its role during IRI is still unclear. Here, by combining two different bioinformatical methods--a method based on ordinary differential equations (Time S...

journal_title：FEBS letters

authors： Le Pape S,Dimitrova E,Hannaert P,Konovalov A,Volmer R,Ron D,Thuillier R,Hauet T

更新日期：2014-08-25 00:00:00

abstract：:Jasmonic acid (JA) has been shown to be a signaling compound which elicits the production of secondary metabolites including phytoalexins in plants. It has been shown that the phytoalexin production is elicited by exogenously applied JA in rice leaves. We now show that this phytoalexin production by exogenously applie...

journal_title：FEBS letters

authors： Tamogami S,Rakwal R,Kodama O

更新日期：1997-07-21 00:00:00

abstract：:We examined the spatial expression patterns of two orphan receptor guanylyl cyclase genes OlGC4 and OlGC5 during embryogenesis of medaka and characterized the 5' flanking region required for tissue-specific expression of OlGC4 by introducing promoter-GFP fusion constructs into medaka embryos. Expression of OlGC5 is co...

journal_title：FEBS letters

authors： Kusakabe T,Suzuki N

更新日期：2000-10-20 00:00:00

abstract：:Localisation of mRNA encoding the four AMPA glutamate receptor subunits in the rat adrenal gland was investigated using in situ hybridisation. GluR1 is found in the zona glomerulosa of the cortex, GluR3 in the remaining parts of the cortex, GluR2 in adrenal medullary cells and GluR4 at a very low level in the zona glo...

journal_title：FEBS letters

authors： Kristensen P

更新日期：1993-10-11 00:00:00

abstract：:Secretoneurin is a recently characterized neuropeptide present in the primary amino acid sequence of secretogranin II. We investigated the proteolytic processing of secretogranin II by prohormone convertases in vivo in a cellular system using the vaccinia virus system. Both PC1 and PC2 can cleave the secretogranin II ...

journal_title：FEBS letters

authors： Hoflehner J,Eder U,Laslop A,Seidah NG,Fischer-Colbrie R,Winkler H

更新日期：1995-03-06 00:00:00

abstract：:The role of two positively charged amino acid residues located at the active site of Escherichia coli maltodextrin phosphorylase was investigated by site-directed mutagenesis. Substitution of Lys539 by an arginine caused a 600-fold reduction, substitution of Arg534 by a glutamine caused an even larger 7000-fold reduct...

journal_title：FEBS letters

authors： Schinzel R,Drueckes P

更新日期：1991-07-29 00:00:00

abstract：:A large protein complex (approx. 2000 kDa) was found in the cytosol of the hyperthermophilic archaebacterium Staphylothermus marinus. The purified protein was shown to be a homomultimer of 93 kDa subunits, the primary structure of which was determined by nucleotide sequence analysis. The protein belongs to the family ...

journal_title：FEBS letters

authors： Cicicopol C,Peters J,Kellermann J,Baumeister W

更新日期：1994-12-19 00:00:00

abstract：:The sequence of seminalplasmin, a basic antimicrobial and transcription-inhibitory protein from bovine seminal plasma, has been determined using an automated sequenator. This sequence is slightly different from that reported earlier by Theil and Scheit [(1983) EMBO J. 2, 1159-1163] and identical with that of caltrin, ...

journal_title：FEBS letters

authors： Sitaram N,Kumari VK,Bhargava PM

更新日期：1986-06-09 00:00:00

abstract：:Temporary inhibition of the cysteine proteinases papain and cathepsin L was observed with several hairpin loop mutants of recombinant chicken cystatin at enzyme concentrations above nanomolar. Kinetic modelling of inhibition data, gel electrophoresis and amino acid sequencing revealed that reappearance of papain activ...

journal_title：FEBS letters

authors： Machleidt W,Nägler DK,Assfalg-Machleidt I,Stubbs MT,Fritz H,Auerswald EA

更新日期：1995-03-20 00:00:00

abstract：:The known functions of type II thioesterases (TEIIs) in type I polyketide synthases (PKSs) include selecting of starter acyl units, removal of aberrant extender acyl units, releasing of final products, and dehydration of polyketide intermediates. In this study, we characterized two TEIIs (ScnI and PKSIaTEII) from Stre...

journal_title：FEBS letters

authors： Wang YY,Ran XX,Chen WB,Liu SP,Zhang XS,Guo YY,Jiang XH,Jiang H,Li YQ

更新日期：2014-08-25 00:00:00

abstract：:In Clostridium thermoaceticum, the synthesis of acetyl-CoA from methyl tetrahydrofolate occurs via a series of enzymatic reactions involving methyl transferase, corrinoid/Fe-S protein (corrinoid), carbon monoxide dehydrogenase (CODH) and ferredoxin. We have investigated the possibility of one or more of these proteins...

journal_title：FEBS letters

authors： Shanmugasundaram T,Sundaresh CS,Kumar GK

更新日期：1993-07-12 00:00:00