Abstract:
:Magnesium (Mg(2+)) transport across membranes plays an essential role in cellular growth and survival. TRPM7 is the unique fusion of a Mg(2+) permeable pore with an active cytosolic kinase domain, and is considered a master regulator of cellular Mg(2+) homeostasis. We previously found that the genetic deletion of TRPM7 in DT40 B cells results in Mg(2+) deficiency and severe growth impairment, which can be rescued by supplementation with excess extracellular Mg(2+). Here, we show that gene expression of the Mg(2+) selective transporter MagT1 is upregulated in TRPM7(-/-) cells. Furthermore, overexpression of MagT1 in TRPM7(-/-) cells augments their capacity to uptake Mg(2+), and improves their growth behavior in the absence of excess Mg(2+).
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Deason-Towne F,Perraud AL,Schmitz Cdoi
10.1016/j.febslet.2011.05.052subject
Has Abstractpub_date
2011-07-21 00:00:00pages
2275-8issue
14eissn
0014-5793issn
1873-3468pii
S0014-5793(11)00418-2journal_volume
585pub_type
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