Abstract:
:DNA bending is due to two main factors: the inherent curvature of the sequence and its flexibility. Most methods of analysis do not allow a differentiation between these two factors. In this paper I show that the flexibility of DNA sequences can be estimated from the standard deviation of roll values determined by X-ray crystallography for each base step. As an application of this approach, the nucleosome formation ability of triple repeat sequences has been determined and shown to be in agreement with the experimental results. Local variations in twist do not appear to have any influence on nucleosome formation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Subirana JAdoi
10.1016/s0014-5793(97)01317-3subject
Has Abstractpub_date
1997-11-17 00:00:00pages
352-4issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(97)01317-3journal_volume
417pub_type
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