The uremic solute indoxyl sulfate acts as an antioxidant against superoxide anion radicals under normal-physiological conditions.

abstract：:In order to study the transactivational property of the X gene in the methylotropic yeast Pichia pastoris, a Rous sarcoma virus-chloramphenicol acetyltransferase (RSV-CAT) cassette was co-transformed and integrated into the host yeast strain as a reporter which showed an overwhelming CAT activity. Immunoprecipitation ...

journal_title：FEBS letters

authors： Lal SK,Kumar V

更新日期：1999-07-30 00:00:00

abstract：:Expression of the leptin receptor gene has been examined in mouse hypothalamus and other brain regions by in situ hybridization. With a probe recognizing all the known splice variants, receptor mRNA was evident in several brain regions (cortex, hippocampus, thalamus), with strong expression in the hypothalamus (arcuat...

journal_title：FEBS letters

authors： Mercer JG,Hoggard N,Williams LM,Lawrence CB,Hannah LT,Trayhurn P

更新日期：1996-06-03 00:00:00

abstract：:Structural integrity may be needed for the glutathione-linked peroxidase activity of human ceruloplasmin. Intact human ceruloplasmin has a potent peroxidase property to decompose H2O2 in the presence of reduced glutathione. However, the fragment of approximately 116000 Da produced by proteolytic degradation had less t...

journal_title：FEBS letters

authors： Kim IG,Park SY

更新日期：1998-10-23 00:00:00

abstract：:The key mechanism in prion disease is the conversion of cellular prion protein into an altered, pathogenic conformation, in which cellular mechanisms play a poorly understood role. Both forms of prion protein are lipid-anchored and reside in rafts that appear to protect the native conformation against conversion. Neur...

journal_title：FEBS letters

authors： Morris RJ,Parkyn CJ,Jen A

更新日期：2006-10-09 00:00:00

abstract：:The phosphopeptide Ser (P)-Ser(P)-Ser-(P)-Glu-Glu-Ser22-Ile-Thr, reproducing the 17-24 segment of beta-casein A2 including the seryl residue (Ser-22) which is targeted by casein kinase-1 was synthesized and used as model substrate for this enzyme. Its phosphorylation efficiency is actually higher than that of intact b...

journal_title：FEBS letters

authors： Meggio F,Perich JW,Reynolds EC,Pinna LA

更新日期：1991-06-03 00:00:00

abstract：:APE-independent base excision repair (BER) pathway plays an important role in the regulation of DNA repair mechanisms. In this study it has been found that recently discovered tyrosyl-DNA phosphodiesterase 1 (Tdp1) catalyzes the AP site cleavage reaction to generate breaks with the 3'- and 5'-phosphate termini. The re...

journal_title：FEBS letters

authors： Lebedeva NA,Rechkunova NI,Lavrik OI

更新日期：2011-02-18 00:00:00

abstract：:The degree of conservation of HeLa interphase chromatin nonhistone antigens among the nonhistones of isolated metaphase chromosomes was determined with immunological procedures. Proteins were separated on SDS-polyacrylamide gels and electrophoretically transferred to diazophenylthioether (DPT)-paper, which was then ov...

journal_title：FEBS letters

authors： Adolph KW

更新日期：1984-01-09 00:00:00

abstract：:Piriprost and nordihydroguiaretic acid (NDGA), specific inhibitors of arachidonate lipoxygenase, inhibited phytohaemagglutinin (PHA)-stimulated breakdown of inositol lipids in human T lymphocytes. The dual inhibitors eicosatetraynoic acid (ETYA) and BW 755C, which inhibit both lipoxygenase and cyclooxygenase, also had...

journal_title：FEBS letters

authors： Mire-Sluis AR,Cox CA,Hoffbrand AV,Wickremasinghe RG

更新日期：1989-11-20 00:00:00

abstract：:Friedreich's ataxia is caused by a deficit in the mitochondrial protein frataxin. The present work demonstrates that in vivo yeast frataxin Yfh1p and Isu1p, the mitochondrial scaffold protein for the Fe-S cluster assembly, have tightly linked biological functions, acting in concert to promote the Fe-S cluster assembly...

journal_title：FEBS letters

authors： Ramazzotti A,Vanmansart V,Foury F

更新日期：2004-01-16 00:00:00

abstract：:Gene expression is controlled through a complex interplay among mRNAs, non-coding RNAs and RNA-binding proteins (RBPs), which all assemble along with other RNA-associated factors in dynamic and functional ribonucleoprotein complexes (RNPs). To date, our understanding of RBPs is largely limited to proteins with known o...

journal_title：FEBS letters

authors： Faoro C,Ataide SF

更新日期：2014-10-16 00:00:00

abstract：:The present study discusses the efficacy of Aloe emodin-8-O-glycoside (AEG), a plant derived anthroquinone, on alleviating insulin resistance and augmenting glycogen synthesis in L6 myotubes and 3T3L1 adipocytes. Dose-dependent increase in glucose uptake activity (GUA) was observed in both cell lines. Immunoblot analy...

journal_title：FEBS letters

authors： Anand S,Muthusamy VS,Sujatha S,Sangeetha KN,Bharathi Raja R,Sudhagar S,Poornima Devi N,Lakshmi BS

更新日期：2010-07-16 00:00:00

abstract：:ZAP-70 is a protein tyrosine kinase (PTK) that plays a critical role in T cell activation. To study the role of ZAP-70 catalytic activity in this process, a substrate capable of distinguishing between the activities of ZAP-70 and other PTKs would be useful, especially since it has recently been shown that ZAP-70 inter...

journal_title：FEBS letters

authors： Watts JD,Brabb T,Bures EJ,Wange RL,Samelson LE,Aebersold R

更新日期：1996-12-02 00:00:00

abstract：:Muscarinic acetylcholine receptors purified from porcine atrium were phosphorylated, depending on the presence of agonists, by a protein kinase partially purified from porcine brain, which had similar properties to the beta-adrenergic receptor kinase. GTP-binding regulatory proteins (Go) had dual effects on the phosph...

journal_title：FEBS letters

authors： Haga K,Haga T

更新日期：1990-07-30 00:00:00

abstract：:F(0)F(1)-ATP synthase couples ATP synthesis/hydrolysis with transmembrane proton transport. The catalytic mechanism involves rotation of the gamma epsilon c(approximately 10)-subunits complex relative to the rest of the enzyme. In the absence of protonmotive force the enzyme is inactivated by the tight binding of MgAD...

journal_title：FEBS letters

authors： Feniouk BA,Junge W

更新日期：2005-09-26 00:00:00

abstract：:A cDNA clone encoding cholesterol side-chain cleavage cytochrome P450 (P450scc) was isolated from a rainbow trout ovarian follicle cDNA library. The cDNA contains an open reading frame of 1,542 nucleotides encoding a protein of 514 amino acids. The predicted amino acid sequence of trout P450scc shows 48% homology with...

journal_title：FEBS letters

authors： Takahashi M,Tanaka M,Sakai N,Adachi S,Miller WL,Nagahama Y

更新日期：1993-03-15 00:00:00

abstract：:Hydrogenase gene from Clostridium butyricum was cloned in Escherichia coli HK16 (Hyd-) using pBR322 and PstI. The plasmid, pCBH1, containing hydrogenase gene was 7.3 MDa and pCBH1 had 5 PstI-DNA fragments (3.9, 2.6, 0.7, 0.03-0.04, less than 0.02 MDa, respectively). The hydrogenase activity of HK16 (pCBH1) was about 3...

journal_title：FEBS letters

authors： Karube I,Urano N,Yamada T,Hirochika H,Sakaguchi K

更新日期：1983-07-11 00:00:00

abstract：:Metabolic control theory is extended to include channelled metabolism in general. A simple relationship between the flux control by the enzymes and the degree of metabolite channelling is derived. This relationship suggests experiments in which modulation of gene expression allows one to quantify channelling. ...

journal_title：FEBS letters

authors： Kholodenko BN,Westerhoff HV

更新日期：1993-03-29 00:00:00

abstract：:Heparin was found to stimulate the phosphorylation of histone H1 but not protamine sulfate catalyzed by Ca2+/phospholipid-dependent protein kinase (protein kinase C or PKC). The effect of heparin on histone H1 phosphorylation appeared to be due to an increase in phosphatidylserine affinity for PKC activation in the pr...

journal_title：FEBS letters

authors： Goueli SA,Hanten JA,Ahmed K

更新日期：1991-05-06 00:00:00

abstract：:The role of Na+/H+ exchange in protein kinase C-mediated effects in platelets was investigated by studying the effect of removal of extracellular Na+ ([Na+]e) on the different responses induced by phorbol 12-myristate 13-acetate (PMA) and 1,2-dioctanoylglycerol (diC8). None of the responses studied, namely, protein ph...

journal_title：FEBS letters

authors： Krishnamurthi S,Patel Y,Morgan WA,Wheeler-Jones CP,Kakkar VV

更新日期：1989-07-31 00:00:00

abstract：:Complex I (NADH:ubiquinone oxidoreductase) is the first and largest protein complex of the oxidative phosphorylation. Crystal structures have elucidated the positions of most subunits of bacterial evolutionary origin in the complex, but the positions of the eukaryotic subunits are unknown. Based on the analysis of seq...

journal_title：FEBS letters

authors： Szklarczyk R,Wanschers BF,Nabuurs SB,Nouws J,Nijtmans LG,Huynen MA

更新日期：2011-03-09 00:00:00

abstract：:The hirudin-like factor 1 (HLF1) of Hirudo medicinalis belongs to a new class of leech-derived factors. In previous investigations, HLF1 did not exhibit anticoagulatory activities. Here, we describe the analysis of natural and synthetic variants of HLF1 and HLF-Hyb, a yet uncharacterized member of the HLF family. Modi...

journal_title：FEBS letters

authors： Müller C,Lukas P,Böhmert M,Hildebrandt JP

更新日期：2020-03-01 00:00:00

abstract：:Olfactory cilia contain cyclic nucleotide-gated and Ca2+-dependent Cl- conductances that underlie excitatory chemotransduction, and a Ca2+-dependent K+ (KCa) conductance, apparently involved in inhibitory transduction. Previous single-channel patch-clamp studies on olfactory cilia revealed four different KCas, with di...

journal_title：FEBS letters

authors： Castillo K,Bacigalupo J,Wolff D

更新日期：2005-03-14 00:00:00

abstract：:The purpose of the present study was to investigate whether 5-hydroxytryptamine (5-HT, serotonin) affects migration of vascular endothelial cells. 5-HT significantly enhanced migration of human aortic endothelial cells (HAECs), and this enhancement was completely inhibited by GR 55562, a 5-HT1 receptor antagonist, and...

journal_title：FEBS letters

authors： Matsusaka S,Wakabayashi I

更新日期：2005-12-19 00:00:00

abstract：:Sialic acids comprise a family of terminal sugars essential for a variety of biological recognition systems. UDP-N-acetylglucosamine 2-epimerase catalyzes the first step of their biosynthesis. Periodate-oxidized UDP-N-acetylglucosamine, namely 2',3'-dialdehydo-UDP-alpha-D-N-acetylglucosamine, was found to be an effect...

journal_title：FEBS letters

authors： Blume A,Chen H,Reutter W,Schmidt RR,Hinderlich S

更新日期：2002-06-19 00:00:00

abstract：:The Alanine Serine Cysteine Transporter 2 (ASCT2) is involved in balancing the intracellular amino acid pool. This function is allowed by the antiport mechanism and the asymmetric specificity towards different neutral amino acids, distinctive of this transporter. In the present work, the interaction of the putative su...

journal_title：FEBS letters

authors： Scalise M,Pochini L,Pingitore P,Hedfalk K,Indiveri C

更新日期：2015-11-30 00:00:00

abstract：:The UDP-GlcNAc:lysosomal enzyme, N-acetylglucosamine-1-phosphotransferase (GlcNAc-1-PT), is an α2 β2 γ2 hexamer that mediates the initial step in the formation of the mannose 6-phosphate targeting signal on newly synthesized lysosomal acid hydrolases. The GNPTAB gene encodes the 1256 amino acid long α/β precursor whic...

journal_title：FEBS letters

authors： Liu L,Lee WS,Doray B,Kornfeld S

更新日期：2017-01-01 00:00:00

abstract：:In rat brain astroglia-enriched cultures long-term treatment with interleukin-1beta induces NO release and stimulation of soluble guanylyl cyclase. The cGMP formed is recovered in the extracellular medium but not in the cell monolayer. The interleukin-1beta effect is mediated by type I receptor and potentiated by inte...

journal_title：FEBS letters

authors： Pedraza CE,Baltrons MA,García A

更新日期：2001-11-02 00:00:00

abstract：:The alpha3/beta4 subtype of neuronal nicotinic acetylcholine receptor (nAChR) was stably expressed in human embryonic kidney (HEK) 293 cells that co-expressed a voltage-gated Ca2+ channel. alpha3/beta4-nAChR-expressing clones were identified using the fura-2 Ca2+ imaging technique, and were further characterised by si...

journal_title：FEBS letters

authors： Stetzer E,Ebbinghaus U,Storch A,Poteur L,Schrattenholz A,Kramer G,Methfessel C,Maelicke A

更新日期：1996-11-11 00:00:00

abstract：:Calpain, a Ca2+-dependent biomodulator, alters the properties of substrate proteins by cleaving them at a limited number of specific sites. Recent studies of the structure-function relationship of calpain and X-ray analysis of its Ca2+-binding domain have revealed hitherto unknown features of the regulation of calpain...

journal_title：FEBS letters

authors： Suzuki K,Sorimachi H

更新日期：1998-08-14 00:00:00

abstract：:The tomato saponin alpha-tomatine has been proposed to kill sensitive cells by binding to cell membranes followed by leakage of cell components. However, details of the modes of action of the compound on fungal cells are poorly understood. In the present study, mechanisms involved in alpha-tomatine-induced cell death ...

journal_title：FEBS letters

authors： Ito S,Ihara T,Tamura H,Tanaka S,Ikeda T,Kajihara H,Dissanayake C,Abdel-Motaal FF,El-Sayed MA

更新日期：2007-07-10 00:00:00