Abstract:
:Okadaic acid, a specific inhibitor of protein phosphatase 1 in Paramecium causes sustained backward swimming in response to depolarising stimuli (S. Klumpp et al. (1990) EMBO J. 9, 685). Here, we employ okadaic acid, tautomycin, microcystin LR and inhibitor 1 as phosphatase inhibitors to identify a 42 kDa protein in the excitable ciliary membrane that is dephosphorylated by protein phosphatase 1. Identification of the 42 kDa protein was facilitated by the finding that the protein kinase responsible for its phosphorylation uses Ca-ATP as a substrate just as effectively as Mg-ATP. Notably, dephosphorylation of the 42 kDa protein is specifically inhibited by cyclic AMP; cyclic GMP has no effect.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Klumpp S,Schultz JEdoi
10.1016/0014-5793(91)81003-qkeywords:
subject
Has Abstractpub_date
1991-08-19 00:00:00pages
60-4issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(91)81003-Qjournal_volume
288pub_type
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