Ceramide generated by acidic sphingomyelinase contributes to tumor necrosis factor-alpha-mediated apoptosis in human colon HT-29 cells through glycosphingolipids formation. Possible role of ganglioside GD3.


:In the present study we assessed the contribution of acidic sphingomyelinase (ASMase), a ceramide generating enzyme, in tumor necrosis factor (TNF)-mediated apoptosis in human colon HT-29 cells. TNF induced apoptosis in HT-29 cells in a time- and dose-dependent fashion. Downregulation of the active endogenous ASMase form prevented TNF-stimulated ASMase activity and apoptosis. Furthermore, inhibition of glucosylceramide synthase, which blunted TNF-stimulated GD3 levels, abolished TNF-mediated cell death. Immunocytochemical staining revealed the co-localization of GD3 with mitochondria induced by TNF. The knockdown of targeted GD3 synthase by antisense expression vector protected HT-29 cells against TNF-induced cell death. Thus, ASMase plays a key role in TNF-induced cell death in human colon epithelial cells possibly through GD3 generation.




FEBS letters


Colell A,Morales A,Fernández-Checa JC,García-Ruiz C





Has Abstract


2002-08-28 00:00:00














  • Fraktionierung handelsüblichen insulins. Das verhalten von proinsulin in der ultrazentrifuge.

    abstract::Bovine insulin can be split into several fractions by gel filtration on Sephadex G50. These are, in order of decreasing molecular weight, a new unidentified substance, proinsulin, and a large fraction containing several substances. Ultracentrifugal examination of the proinsulin in acetic acid solution (pH 2.3) gave a ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zühlke H,Behlke J

    更新日期:1968-12-01 00:00:00

  • Multisite phosphorylation of the 80 kDa (MARCKS) protein kinase C substrate in C3H/10T1/2 fibroblasts. Quantitative analysis of individual sites by solid-phase microsequencing.

    abstract::A synthetic peptide, KKKKRFSFKKSFKLSGFSFKK, containing the phosphorylation sites of the acidic 80-87 kDa protein kinase C substrate was used to identify phosphopeptides in enzyme digests of this protein from mouse fibroblast C3H/10T1/2 cells. Stimulation of phosphorylation occurred, in vivo, with TPA at Ser7, Ser11 an...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Amess B,Manjarrez-Hernandez HA,Howell SA,Learmonth M,Aitken A

    更新日期:1992-02-10 00:00:00

  • Prominent expression of FRS2beta protein in neural cells and its association with intracellular vesicles.

    abstract::The fibroblast growth factor receptor substrate (FRS)-2 protein family comprises FRS2alpha, a well-known central mediator for fibroblast growth factor signaling, and FRS2beta, whose endogenous expression pattern and function are not yet defined. Immunohistochemical analysis revealed that expression of FRS2beta was res...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Minegishi Y,Iwanari H,Mochizuki Y,Horii T,Hoshino T,Kodama T,Hamakubo T,Gotoh N

    更新日期:2009-02-18 00:00:00

  • The two common polymorphic forms of human NRH-quinone oxidoreductase 2 (NQO2) have different biochemical properties.

    abstract::There are two common forms of NRH-quinone oxidoreductase 2 (NQO2) in the human population resulting from SNP rs1143684. One has phenylalanine at position 47 (NQO2-F47) and the other leucine (NQO2-L47). Using recombinant proteins, we show that these variants have similar steady state kinetic parameters, although NQO2-L...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Megarity CF,Gill JR,Caraher MC,Stratford IJ,Nolan KA,Timson DJ

    更新日期:2014-05-02 00:00:00

  • Unfolding of bacteriophage P22 tailspike protein: enhanced thermal stability of an N-terminal fusion mutant.

    abstract::The tailspike protein (TSP) of bacteriophage P22 is a homotrimeric multifunctional protein responsible for cell attachment and hydrolysis of the Salmonella typhimurium host cell receptor. Despite the folding of TSP involves the formation of thermolabile intermediates, the mature protein is extremely resistant to heat ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Carbonell X,Villaverde A

    更新日期:1998-08-07 00:00:00

  • Identification and structural characterization of a mannose-6-phosphate containing oligomannosidic N-glycan from human erythropoietin secreted by recombinant BHK-21 cells.

    abstract::A sialidase resistant mono-charged N-glycan was isolated from glycosylation site I (Asn-24) of recombinant human erythropoietin expressed from baby hamster kidney (BHK-21) cells and constituted approximately 2-4% of the oligosaccharide material at this glycosylation site. Mass spectrometry and both 1- and 2-dimensiona...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nimtz M,Wray V,Rüdiger A,Conradt HS

    更新日期:1995-05-29 00:00:00

  • Positive feedback between ethanolamine-specific phospholipid base exchange and cytochrome P450 activities in rat liver microsomes. The effect of clofibric acid.

    abstract::The results of the present investigation relate the effects of the nutritional state and administration of clofibric acid (CLA), a hypolipidaemic drug and peroxisomal proliferator, on phosphatidylethanolamine (PE) synthesis in rat liver and fatty acid metabolism. Fasting and CLA treatment of animals causes an increase...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lenart J,Komańska I,Pikuła S,Jasińska R

    更新日期:1998-08-28 00:00:00

  • Alteration of transbilayer phospholipid compositions is involved in cell adhesion, cell spreading, and focal adhesion formation.

    abstract::We previously showed that P4-ATPases, ATP10A/ATP8B1, and ATP11A/ATP11C have flippase activities toward phosphatidylcholine (PC), and aminophospholipids [phosphatidylserine (PS) and phosphatidylethanolamine], respectively. Here, we investigate the effect of PC-specific flippases versus aminophospholipid-specific flippa...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Miyano R,Matsumoto T,Takatsu H,Nakayama K,Shin HW

    更新日期:2016-07-01 00:00:00

  • Activation of Na,K-ATPase by an endogenous peptide, PEC-60.

    abstract::Several peptidic and non-peptidic factors can modulate Na,K-ATPase activity, among them mainly inhibitors of this enzyme, ouabain being the most effective. In a very few cases only, activation of Na,K-ATPase by endogenous factors has been recorded. We have investigated the effect on Na,K-ATPase of a novel regulatory p...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kairane C,Zilmer M,Mutt V,Sillard R

    更新日期:1994-05-23 00:00:00

  • A pseudosubstrate peptide inhibits protein kinase C-mediated phosphorylation in permeabilized Rat-1 cells.

    abstract::Activation of protein kinase C (PKC) in Rat-1 fibroblasts leads to rapid phosphorylation of an 80-kDa protein, a major substrate of PKC. Digitonin-permeabilized cells perfectly supported this early response. Introduction of a PKC pseudosubstrate peptide inhibited 80 kDa phosphorylation with an IC50 of 1 microM, while ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Eichholtz T,Alblas J,van Overveld M,Moolenaar W,Ploegh H

    更新日期:1990-02-12 00:00:00

  • MiR-449c targets c-Myc and inhibits NSCLC cell progression.

    abstract::MicroRNAs (miRNA) play an important role in tumorigenesis, proliferation, and differentiation. Altered miRNA expression in cancer indicates that miRNAs can function as tumor suppressors or oncogenes. MiR-449c downregulation in non-small cell lung cancer (NSCLC) compared with normal lung tissues was investigated in thi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Miao LJ,Huang SF,Sun ZT,Gao ZY,Zhang RX,Liu Y,Wang J

    更新日期:2013-05-02 00:00:00

  • Periplasmic competition for zinc uptake between the metallochaperone ZnuA and Cu,Zn superoxide dismutase.

    abstract::We have investigated the availability of zinc in the periplasmic space of Escherichia coli using a mutant Cu,Zn superoxide dismutase whose dimerization is triggered by zinc binding. This mutant enzyme accumulates in the monomeric form when wild type cells are grown in minimal medium, but assembles in the dimeric form ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Berducci G,Mazzetti AP,Rotilio G,Battistoni A

    更新日期:2004-07-02 00:00:00

  • Time course and temperature dependence of synaptic vesicle endocytosis.

    abstract::In presynaptic nerve terminals, synaptic vesicles are recycled locally via an evolutionarily conserved process that ensures maintenance of neurotransmission as well as structural integrity of synapses. Temperature is a key environmental factor that impacts critical steps involved in fusion, endocytosis and transport i...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Chanaday NL,Kavalali ET

    更新日期:2018-11-01 00:00:00

  • Genetic code preferentially conserves long-range interactions among the amino acids.

    abstract::The physical properties of amino acids were investigated in order to evaluate their possible relationship to the assignment of codons for amino acids in the genetic code. A comparison of the interconversion probability between amino acids and the distances between the amino acids for individual physical properties rev...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sitaramam V

    更新日期:1989-04-10 00:00:00

  • Isolation and analysis of the mouse opsin gene.

    abstract::We have identified three overlapping 5'-truncated mouse opsin cDNA clones by immunologically screening a lambda gt11 retina expression library. Using one of the cDNA clones as a probe, we isolated a 5 kb genomic fragment that encompassed the complete coding sequence for mouse opsin. The coding region for opsin was int...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Baehr W,Falk JD,Bugra K,Triantafyllos JT,McGinnis JF

    更新日期:1988-10-10 00:00:00

  • In vivo translational efficiency of different hepatitis C virus 5'-UTRs.

    abstract::Initiation of translation in hepatitis C virus (HCV) is dependent on the presence of an internal ribosome entry site (IRES) contained in its 341-nt-long 5'-untranslated region (UTR). This region is very conserved among different isolates and has been used to classify HCV isolates in six different genotypes. These geno...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Buratti E,Gerotto M,Pontisso P,Alberti A,Tisminetzky SG,Baralle FE

    更新日期:1997-07-14 00:00:00

  • Role of prostaglandin E2 in peptidoglycan mediated iNOS expression in mouse peritoneal macrophages in vitro.

    abstract::Many extracellular stimuli, e.g. microbial products, cytokines etc., result in the expression of inducible nitric oxide synthase (iNOS) in macrophages. However, it is not known whether expression of the iNOS gene in response to microbial products is a primary response of macrophages, or is the result of paracrine/auto...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dahiya Y,Pandey RK,Bhatt KH,Sodhi A

    更新日期:2010-10-08 00:00:00

  • Complex demethylation patterns at Sp1 binding sites in F9 embryonal carcinoma cells.

    abstract::The ubiquitous transcription factor Sp1 has been implicated in the mechanism which maintains CpG islands methylation-free. Plasmids containing GC boxes (Sp1 sites) were in vitro methylated at every CpG dinucleotide. After stable introduction into F9 embryonal carcinoma cells, we analysed the methylation of the sequenc...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Silke J,Rother KI,Georgiev O,Schaffner W,Matsuo K

    更新日期:1995-08-21 00:00:00

  • A new FACIT of the collagen family: COL21A1.

    abstract::Interrogation of the Human Genome data for sequences related to the von Willebrand factor A-domain module identified a previously unreported 4.1 kb full-length cDNA that is predicted to encode a new member of the collagen superfamily of extracellular matrix proteins, collagen XXI. The domain organization of collagen X...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fitzgerald J,Bateman JF

    更新日期:2001-09-14 00:00:00

  • Serum amyloid A and protein AA: molecular mechanisms of a transmissible amyloidosis.

    abstract::Systemic AA-amyloidosis is a complication of chronic inflammatory diseases and the fibril protein AA derives from the acute phase reactant serum AA. AA-amyloidosis can be induced in mice by an inflammatory challenge. The lag phase before amyloid develops can be dramatically shortened by administration of a small amoun...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Westermark GT,Westermark P

    更新日期:2009-08-20 00:00:00

  • The isolated N-terminal extracellular domain of the glucagon-like peptide-1 (GLP)-1 receptor has intrinsic binding activity.

    abstract::The glucagon-like peptide 1 (7-37)/(7-36) amide (GLP-1) receptor belongs to a new subclass of seven transmembrane domain, G-protein coupled receptors comprising several receptors for peptide hormones. The receptors of this family share many common motifs including a relatively large N-terminal extracellular domain. Th...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wilmen A,Göke B,Göke R

    更新日期:1996-11-25 00:00:00

  • Involvement of a 65 kDa phosphoprotein in the regulation of membrane fusion during exocytosis in Paramecium cells.

    abstract::Antisera were raised against a phosphoprotein of 65 kDa (PP65) from Paramecium cells (shown before to be selectively dephosphorylated during synchronous exocytosis) and specified by immunoblotting. By immunofluorescence PP65 has been localized within the cortex, beneath the cell membrane. This corresponds to data obta...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Stecher B,Höhne B,Gras U,Momayezi M,Glas-Albrecht R,Plattner H

    更新日期:1987-10-19 00:00:00

  • Characterization of the wound-induced metallocarboxypeptidase inhibitor from potato. cDNA sequence, induction of gene expression, subcellular immunolocalization and potential roles of the C-terminal propeptide.

    abstract::A partial cDNA clone for the potato wound-inducible metallocarboxypeptidase inhibitor (PCI) was isolated from a cDNA library constructed from mRNA of abscisic acid (ABA)-treated potato leaves. The full 5' region of the cDNA was obtained through a RACE-PCR protocol. PCI mRNA encodes a precursor polypeptide which compri...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Villanueva J,Canals F,Prat S,Ludevid D,Querol E,Avilés FX

    更新日期:1998-11-27 00:00:00

  • Buthionine sulfoximine is a multitarget inhibitor of trypanothione synthesis in Trypanosoma cruzi.

    abstract::Buthionine sulfoximine (BSO) induces decreased glutathione (GSH) and trypanothione [T(SH)2 ] pools in trypanosomatids, presumably because only gamma-glutamylcysteine synthetase (γECS) is blocked. However, some BSO effects cannot be explained by exclusive γECS inhibition; therefore, its effect on the T(SH)2 metabolism ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vázquez C,Mejia-Tlachi M,González-Chávez Z,Silva A,Rodríguez-Zavala JS,Moreno-Sánchez R,Saavedra E

    更新日期:2017-12-01 00:00:00

  • Mitochondrial dynamics, mitophagy and biogenesis in neonatal hypoxic-ischaemic brain injury.

    abstract::Hypoxic-ischaemic encephalopathy, resulting from asphyxia during birth, affects 2-3 in every 1000 term infants and depending on severity, brings about life-changing neurological consequences or death. This hypoxic-ischaemia (HI) results in a delayed neural energy failure during which the majority of brain injury occur...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Thornton C,Jones A,Nair S,Aabdien A,Mallard C,Hagberg H

    更新日期:2018-03-01 00:00:00

  • Urea-generated free rotating water molecules are active in the protein unfolding process.

    abstract::The critical urea concentration (C3*) which destabilizes the structure of bovine serum albumin and chymotrypsinogen was determined by UV difference spectroscopy. The increase of the relative content of mobile rotating water molecules in aqueous urea was formerly shown by millimeter spectroscopy [1]. The rise of rotato...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Khurgin Yu,Maksareva E

    更新日期:1993-01-04 00:00:00

  • Activation of endogenous c-Src or a related tyrosine kinase by intracellular (pY)EEI peptide increases voltage-operated calcium channel currents in rabbit ear artery cells.

    abstract::The effect of activation of endogenous c-Src tyrosine kinase by (pY)EEI peptide was examined on voltage-operated calcium channel (VOC) currents in arterial smooth muscle cells. In single rabbit ear artery cells intracellular application of (pY)EEI peptide increased calcium channel currents. Inactive, non-phosphorylate...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wijetunge S,Hughes AD

    更新日期:1996-12-09 00:00:00

  • Vitamin K-dependent carboxylase: synthesis of an inhibitor of the glutamyl binding site.

    abstract::Liver microsomes contain a vitamin K and O2-dependent carboxylase that converts peptide-bound glutamyl residues to gamma-carboxyglutamate residues. The peptide Boc-O-phospho-Ser-O-phospho-Ser-Leu-OMe has now been synthesized. This peptide inhibits the carboxylation of endogenous protein precursors by a detergent-solub...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rich DH,Kawai M,Goodman HL,Engelke J,Suttie JW

    更新日期:1983-02-07 00:00:00

  • Human CyP33 binds specifically to mRNA and binding stimulates PPIase activity of hCyP33.

    abstract::Human nuclear cyclophilin 33 (hCyP33) was the first protein which was found to contain an RNA-binding motif and a PPIase domain. It was not known what cellular and physiological roles are played by the RNA-binding activity as well as the PPIase activity of hCyP33. In this paper, we investigated the binding specificity...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wang Y,Han R,Zhang W,Yuan Y,Zhang X,Long Y,Mi H

    更新日期:2008-03-05 00:00:00

  • Functional analysis of an indole-diterpene gene cluster for lolitrem B biosynthesis in the grass endosymbiont Epichloë festucae.

    abstract::Epichloë festucae Fl1 in association with Lolium perenne synthesizes a diverse range of indole-diterpene bioprotective metabolites, including lolitrem B, a potent tremorgen. The ltm genes responsible for the synthesis of these metabolites are organized in three clusters at a single sub-telomeric locus in the genome of...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Saikia S,Takemoto D,Tapper BA,Lane GA,Fraser K,Scott B

    更新日期:2012-07-30 00:00:00