Suppressors of RNA silencing encoded by plant viruses and their role in viral infections.

abstract：:The ∆Np63 isoform of the p53-family transcription factor Trp63 is a key regulator of mammary epithelial stem cells that is involved in breast cancer development. To investigate the role of ∆Np63 at different stages of normal mammary gland development, we generated a ∆Np63-inducible conditional knockout (cKO) mouse mod...

journal_title：FEBS letters

authors： Kumar S,Nandi A,Mahesh A,Sinha S,Flores E,Chakrabarti R

更新日期：2020-03-01 00:00:00

abstract：:Low-density lipoprotein receptor-related protein 4 (Lrp4) is essential for pre- and post-synaptic specialization at the neuromuscular junction (NMJ), an indispensable synapse between a motor nerve and skeletal muscle. Muscle-specific receptor tyrosine kinase MuSK must form a complex with Lrp4 to organize postsynaptic ...

journal_title：FEBS letters

authors： Hoshi T,Tezuka T,Yokoyama K,Iemura S,Natsume T,Yamanashi Y

更新日期：2013-11-29 00:00:00

abstract：:Helical filaments of bacterial flagella are built up by a self-assembly process from thousands of flagellin subunits. To clarify how the disordered terminal regions of flagellin interact upon filament formation, polymerization ability of various terminally truncated fragments was investigated. Fragments deprived of 19...

journal_title：FEBS letters

authors： Gugolya Z,Muskotál A,Sebestyén A,Diószeghy Z,Vonderviszt F

更新日期：2003-01-30 00:00:00

abstract：:Thrombomodulin (TM) is a single-transmembrane glycoprotein receptor for thrombin, which is best known as a cofactor for thrombin-mediated activation of anticoagulant protein C. C-type lectin-like domain (CTLD) of TM has distinct coagulation/fibrinolysis-independent anti-inflammatory properties. Here we found anti-infl...

journal_title：FEBS letters

authors： Jin H,Yang X,Liu K,Gu Q,Xu X

更新日期：2011-11-04 00:00:00

abstract：:Rats bearing the fast-growing AH-130 Yoshida ascites hepatoma showed a marked cachectic response which has been previously reported [Tessitore et al. (1987) Biochem. J. 241, 153-159]. Thus tumour-bearing animals showed significant decreases in body and muscle weight (soleus and gastrocnemius) as compared to both pair-...

journal_title：FEBS letters

authors： Llovera M,García-Martínez C,Agell N,Marzábal M,López-Soriano FJ,Argilés JM

更新日期：1994-02-07 00:00:00

abstract：:Gene array profiling of RNA from cells engineered to express a dominant-negative version of the ETS family member transcription factor Pea3 (polyomavirus enhancer activator 3) identified WT1 as a candidate downstream gene. Given the co-expression of WT1 and Pea3 in developing kidney and breast tissue undergoing mesenc...

journal_title：FEBS letters

authors： Discenza MT,Vaz D,Hassell JA,Pelletier J

更新日期：2004-02-27 00:00:00

abstract：:Azide-binding to the heme-copper binuclear center of bo-type ubiquinol oxidase from Escherichia coli was investigated with Fourier-transform infrared spectroscopy. Deconvolution analyses of infrared spectra of the azide (14N3)-inhibited air-oxidized form showed a major infrared azide antisymmetric stretching band at 2...

journal_title：FEBS letters

authors： Tsubaki M,Mogi T,Hori H

更新日期：1999-04-23 00:00:00

abstract：:Association reactions of a recombinant CFTR-NBF-2 polypeptide fused to glutathione S-transferase with guanine nucleotides were monitored quantitatively by recording the fluorescence enhancement of excited trinitrophenol (TNP)-labelled GTP after binding to NBF-2. Binding of TNP-GTP to the recombinant NBF-2 polypeptide ...

journal_title：FEBS letters

authors： Randak C,Neth P,Auerswald EA,Assfalg-Machleidt I,Roscher AA,Hadorn HB,Machleidt W

更新日期：1996-11-25 00:00:00

abstract：:Subclinical inflammation is a candidate etiological factor in the pathogenesis of metabolic syndrome and in the progression of atherosclerosis. A central role for activated macrophages has been elucidated recently as important regulators of the inflammatory process in atherosclerosis. Macrophage differentiation and fu...

journal_title：FEBS letters

authors： Szanto A,Roszer T

更新日期：2008-01-09 00:00:00

abstract：:Several positive transcription factors regulate Arabidopsis anthocyanin biosynthesis. HY5, a component of light-signaling pathways, and PAP1, an R2R3-MYB transcription factor, share common regulatory targets on anthocyanin biosynthesis genes. The epistatic interactions between the two transcription factors are current...

journal_title：FEBS letters

authors： Shin DH,Choi M,Kim K,Bang G,Cho M,Choi SB,Choi G,Park YI

更新日期：2013-05-21 00:00:00

abstract：:It has recently been discovered that glutathione-dependent formaldehyde dehydrogenase (FALDH) exhibits a strong S-nitrosoglutathione reductase activity. Plants use NO and S-nitrosothiols as signaling molecules to activate defense mechanisms. Therefore, it is interesting to investigate the regulation of FALDH by mechan...

journal_title：FEBS letters

authors： Díaz M,Achkor H,Titarenko E,Martínez MC

更新日期：2003-05-22 00:00:00

abstract：:Schizosaccharomyces pombe cells take up D-gluconate, as an alternative carbon source for growth, during glucose starvation or when cultured on glycerol-containing medium. Gluconate uptake is not detectable while cells are growing logarithmically on glucose. The addition of D-glucose as well as its non-metabolizable an...

journal_title：FEBS letters

authors： Caspari T,Urlinger S

更新日期：1996-10-21 00:00:00

abstract：:Here we present a method to purify large amounts of highly pure and stably arrested ribosome-nascent chain complexes (RNCs) from Escherichia coli cells. It relies on the combined use of translation-arrest sequences to generate nascent polypeptides of specified length and subsequent tag purification of the RNCs. Moreov...

journal_title：FEBS letters

authors： Rutkowska A,Beerbaum M,Rajagopalan N,Fiaux J,Schmieder P,Kramer G,Oschkinat H,Bukau B

更新日期：2009-07-21 00:00:00

abstract：:The cDNA coding for mature human alpha 1-proteinase inhibitor (alpha 1-PI) has been inserted into a variety of yeast expression vectors. Yeast cells transformed with these plasmids were then assayed for the production of mature, unglycosylated alpha 1-PI. The production level is optimal when the recombinant plasmid ca...

journal_title：FEBS letters

authors： Hoylaerts M,Weyens A,Bollen A,Harford N,Cabezón T

更新日期：1986-08-11 00:00:00

abstract：:A new epsilon-N-trimethyllysine metabolite has been isolated from the mycelium of Neurospora crassa. The labelled compound produced from incubations in vivo and in vitro from epsilon-N-trimethyl ([14-C]H3)L-lysine has been identified as 2-keto-epsilon-N-trimethyl-hexanoic acid by reducing its 2,4-dinitrophenyl hydrazo...

journal_title：FEBS letters

authors： Villanueva VR,Lederer E

更新日期：1975-04-01 00:00:00

abstract：:We previously isolated a Saccharomyces cerevisiae mutant (HsTnII), which displays 40% reduced chronological lifespan as compared to the wild type (WT). In this study, we found HsTnII cultures to be characterized by fragmented and dysfunctional mitochondria, and by increased initiation of apoptosis during chronological...

journal_title：FEBS letters

authors： Aerts AM,Zabrocki P,Govaert G,Mathys J,Carmona-Gutierrez D,Madeo F,Winderickx J,Cammue BP,Thevissen K

更新日期：2009-01-05 00:00:00

abstract：:The morphology of complexes between relaxed circular plasmid pBR322 DNA and tripeptide L-Val-L-Val-L-Val-NH-NH-Dns (TVP) at different peptide/DNA ratios was studied by electron microscopy. The results show that interaction of TVP with circular DNA leads to the formation of perfect torus-shaped particles. The torus par...

journal_title：FEBS letters

authors： Vengerov YY,Semenov TE,Streltsov SA,Makarov VL,Khorlin AA,Gursky GV

更新日期：1985-01-21 00:00:00

abstract：:A latent MAP kinase kinase kinase activity previously detected after chromatography of PC12 cell extracts on Mono Q [(1992) FEBS Lett. 314, 461-465] has been identified as B-Raf by immunoblotting and immunoprecipitation experiments and by its specific activation with B-Raf antibodies. B-Raf is phosphorylated after sti...

journal_title：FEBS letters

authors： Traverse S,Cohen P

更新日期：1994-08-15 00:00:00

abstract：:Extended X-ray absorption fine structure (EXAFS) spectra at the Cu K-edge have been recorded of the oxidized and reduced form at pH 3.5 of rusticyanin, the type-1 or 'blue'-copper protein from Thiobacillus ferrooxidans. The EXAFS of oxidized rusticyanin is well simulated with models assuming a ligand set of 2 N(His) a...

journal_title：FEBS letters

authors： Holt SD,Piggott B,Ingledew WJ,Feiters MC,Diakun GP

更新日期：1990-08-20 00:00:00

abstract：:ADP-ribosylation of HeLa nonhistone proteins was investigated by using [3H]adenosine as an in vivo radioactive label. The aim was to determine basic differences in the patterns of modification of interphase and metaphase nonhistones. Fluorography revealed a relatively small number of modified proteins for isolated met...

journal_title：FEBS letters

authors： Adolph KW,Song MK

更新日期：1985-03-11 00:00:00

abstract：:Bcl-xL, a close homolog of Bcl2, is an important regulator of apoptosis and is overexpressed in human cancer. Phosphorylation of Bcl-xL can be induced by microtubule-damaging drugs such as taxol or 2-methoxyestradiol (2-ME). By site-directed mutagenesis studies, we have identified that serine 62 is the necessary site ...

journal_title：FEBS letters

authors： Basu A,Haldar S

更新日期：2003-03-13 00:00:00

abstract：:The open reading frame YLR070c of Saccharomyces cerevisiae has high sequence similarity to S. cerevisiae sorbitol dehydrogenase and to xylitol dehydrogenase of Pichia stipitis. Overexpression of this open reading frame in S. cerevisiae resulted in xylitol dehydrogenase activity. The enzyme is specific for NADH. The fo...

journal_title：FEBS letters

authors： Richard P,Toivari MH,Penttilä M

更新日期：1999-08-20 00:00:00

abstract：:Two new three-disulfide intermediates have been found to be populated in the oxidative folding pathway of bovine pancreatic ribonuclease A at a low temperature (15 degrees C). These intermediates, des-[26-84] and des-[58-110], possess all but one of the four native disulfide bonds and have a stable tertiary structure,...

journal_title：FEBS letters

authors： Welker E,Narayan M,Volles MJ,Scheraga HA

更新日期：1999-11-05 00:00:00

abstract：:Upon in vitro translation of mRNAs from slow (soleus) muscles of the mouse a hitherto undescribed translation product has been detected that was absent from fast skeletal muscles and was termed p19/6.8 according to its position in 2-dimensional gels. mRNA for p19/6.8 was also found in the ventricle of the heart. p19/6...

journal_title：FEBS letters

authors： Kluxen FW

更新日期：1988-03-28 00:00:00

abstract：:Recently, we reported two novel early-staged encapsulation-relating proteins (56 kDa and 48 kDa ERPs) isolated from the hemolymph of coleopteran insect, Tenebrio molitor larvae [Cho et al. (1999) Eur. J. Biochem. (in press)]. Here, a cDNA clone for another early-staged encapsulation-relating protein (86 kDa) was isola...

journal_title：FEBS letters

authors： Cho MY,Choi HW,Moon GY,Kim MH,Kwon TH,Homma K,Natori S,Lee BL

更新日期：1999-05-28 00:00:00

abstract：:A novel protein that binds specifically to the intracellular domain of the p55 tumor necrosis factor (TNF) receptor was cloned by two-hybrid screening of a HeLa cell cDNA library. Data bank searches revealed high sequence similarity of the protein (55.11) to yeast, nematode and plant proteins, whose functions are yet ...

journal_title：FEBS letters

authors： Boldin MP,Mett IL,Wallach D

更新日期：1995-06-19 00:00:00

abstract：:The profiling of grapevine (Vitis vinifera L.) genes under water deficit was specifically targeted to sugar transporters. Leaf water status was characterized by physiological parameters and soluble sugars content. The expression analysis provided evidence that VvHT1 hexose transporter gene was strongly down-regulated ...

journal_title：FEBS letters

authors： Medici A,Laloi M,Atanassova R

更新日期：2014-11-03 00:00:00

abstract：:A high-throughput cell-free protein synthesis method has been described. The methodology is based on a bilayer diffusion system that enables the continuous supply of substrates, together with the continuous removal of small byproducts, through a phase between the translation mixture and substrate mixture. With the use...

journal_title：FEBS letters

authors： Sawasaki T,Hasegawa Y,Tsuchimochi M,Kamura N,Ogasawara T,Kuroita T,Endo Y

更新日期：2002-03-06 00:00:00

abstract：:Two different clones of Swiss 3T3 cells belonging to the same original cell line have been obtained, one of which was unresponsive to mitogenic stimulation (e.g. insulin-like growth factor-I, bombesin, insulin-like growth factor-I + bombesin), while the other clone showed a very high rate of DNA synthesis under identi...

journal_title：FEBS letters

authors： Martelli AM,Gilmour RS,Neri LM,Manzoli L,Corps AN,Cocco L

更新日期：1991-06-03 00:00:00

abstract：:Poly(A)+ RNA isolated from post-mortem human hypothalami has been used to characterize the poly-protein precursors to vasopressin and oxytocin. Translation in a cell-free system and subsequent immuno-precipitation with antibodies raised against either vasopressin or neurophysin identified a product of Mr 19000 (prepro...

journal_title：FEBS letters

authors： Mohr E,Hillers M,Ivell R,Haulica ID,Richter D

更新日期：1985-11-25 00:00:00