Abstract:
:We have identified a novel protein on the outer membrane of Arabidopsis thaliana mitochondria. This protein displays 67% sequence identity with the 64 kDa translocase of the outer envelope membrane of chloroplasts (Toc). A mitochondrial localisation for this protein was determined by (i). its presence in the proteome of highly purified Arabidopsis mitochondria, (ii). Western blot analysis with antibodies to Toc64 from pea that indicate its presence in Arabidopsis and pea mitochondria, (iii). green fluorescent protein fusion proteins that indicate an exclusive mitochondrial localisation for this protein, and (iv). expression profiles in various tissue types and during development that are more similar to translocase of the outer mitochondrial membrane components than to chloroplastic Toc components. Thus Arabidopsis mitochondria contain a protein with high sequence identity to a plastid protein import receptor.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Chew O,Lister R,Qbadou S,Heazlewood JL,Soll J,Schleiff E,Millar AH,Whelan Jdoi
10.1016/s0014-5793(03)01457-1keywords:
subject
Has Abstractpub_date
2004-01-16 00:00:00pages
109-14issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579303014571journal_volume
557pub_type
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