Error-prone DNA polymerases: novel structures and the benefits of infidelity.

abstract：:NF-kappa B is a nuclear protein, found only in cells that transcribe immunoglobulin light chain genes, that interacts with a defined site in the kappa immunoglobulin enhancer. This protein can be induced in pre-B cells by stimulation with bacterial lipopolysaccharide (LPS). The induction involves a posttranslational a...

journal_title：Cell

authors： Sen R,Baltimore D

更新日期：1986-12-26 00:00:00

abstract：:Four RNA products have been characterized from a soluble system that accurately splices purified substrate RNA. The labeled substrate RNA contained the first and second exons of the major late transcription unit of adenovirus 2 and a shortened form of the first intervening sequence. One of these RNA products was the e...

journal_title：Cell

authors： Grabowski PJ,Padgett RA,Sharp PA

更新日期：1984-06-01 00:00:00

abstract：:A family of novel substrates was designed to enable the efficient accumulation of intermediates in site-specific recombination. Strategically placed nicks allow these "suicide substrates" to initiate the reaction but prevent its completion or reversal. Consequently, it has been possible to determine that lambda site-s...

journal_title：Cell

authors： Nunes-Düby SE,Matsumoto L,Landy A

更新日期：1987-08-28 00:00:00

abstract：:During adaptive angiogenesis, a key process in the etiology and treatment of cancer and obesity, the vasculature changes to meet the metabolic needs of its target tissues. Although the cues governing vascular remodeling are not fully understood, target-derived signals are generally believed to underlie this process. H...

journal_title：Cell

authors： Linneweber GA,Jacobson J,Busch KE,Hudry B,Christov CP,Dormann D,Yuan M,Otani T,Knust E,de Bono M,Miguel-Aliaga I

更新日期：2014-01-16 00:00:00

abstract：:Three different cases of pancreatic beta cell hyperplasia in mice are accompanied by an increase in a subclass of cells expressing tyrosine hydroxylase (TH), a neuronal enzyme. In the nontumorigenic cases of islet growth during normal pregnancy and in the obese mutant mouse, the TH-insulin cells do not divide, in cont...

journal_title：Cell

authors： Teitelman G,Alpert S,Hanahan D

更新日期：1988-01-15 00:00:00

abstract：:In atherosclerosis, the accumulation of apolipoprotein B-lipoproteins in the matrix beneath the endothelial cell layer of blood vessels leads to the recruitment of monocytes, the cells of the immune system that give rise to macrophages and dendritic cells. Macrophages derived from these recruited monocytes participate...

journal_title：Cell

authors： Moore KJ,Tabas I

更新日期：2011-04-29 00:00:00

abstract：:How chaperonins orchestrate the successful folding of even the most elaborate of proteins is a question of central importance. In two recent studies in Cell by Joachimiak et al. and Freund et al., a new class of TRiC substrate is identified, and how the chaperonin exploits its different subunits to extend its substrat...

journal_title：Cell

authors： Zhuravleva A,Radford SE

更新日期：2014-12-04 00:00:00

abstract：:Synapse formation requires changes in cell morphology and the upregulation and localization of synaptic proteins. In the cerebellum, mossy fibers undergo extensive remodeling as they contact several granule cells and form complex, multisynaptic glomerular rosettes. Here we show that granule cells secrete factors that ...

journal_title：Cell

authors： Hall AC,Lucas FR,Salinas PC

更新日期：2000-03-03 00:00:00

abstract：:Nine transcripts complementary to mouse L cell mitochondrial DNA have been detected, sized and mapped to restriction fragments using the method of Berk and Sharp (1977). RNA isolated from L cell mitochondria was hybridized to 32P-labeled, cloned L cell mitochondrial DNA restriction fragments in 70% formamide under con...

journal_title：Cell

authors： Battey J,Clayton DA

更新日期：1978-05-01 00:00:00

abstract：:The hsp 70 gene of Drosophila contains three domains to which a heat shock gene specific transcription factor (HSTF) binds. In addition to the previously described 55 bp binding domain proximal to the TAT homology, there are two 25 bp binding sites farther upstream. Footprinting studies with 5' and 3' deletion mutatio...

journal_title：Cell

authors： Topol J,Ruden DM,Parker CS

更新日期：1985-09-01 00:00:00

abstract：:We determined the nucleotide sequences of all coding regions and a significant part of the flanking regions of the chicken c-src gene, which is a cellular homolog of the v-src gene of Rous sarcoma virus. The c-src gene consists of 12 exons; the boundaries of the exons were determined by assuming that the amino acid se...

journal_title：Cell

authors： Takeya T,Hanafusa H

更新日期：1983-03-01 00:00:00

abstract：:The intestinal microbiota produces tens of thousands of metabolites. Here, we used host sensing of small molecules by G-protein coupled receptors (GPCRs) as a lens to illuminate bioactive microbial metabolites that impact host physiology. We screened 144 human gut bacteria against the non-olfactory GPCRome and identif...

journal_title：Cell

authors： Chen H,Nwe PK,Yang Y,Rosen CE,Bielecka AA,Kuchroo M,Cline GW,Kruse AC,Ring AM,Crawford JM,Palm NW

更新日期：2019-05-16 00:00:00

abstract：:Alu RNA accumulation due to DICER1 deficiency in the retinal pigmented epithelium (RPE) is implicated in geographic atrophy (GA), an advanced form of age-related macular degeneration that causes blindness in millions of individuals. The mechanism of Alu RNA-induced cytotoxicity is unknown. Here we show that DICER1 def...

journal_title：Cell

authors： Tarallo V,Hirano Y,Gelfand BD,Dridi S,Kerur N,Kim Y,Cho WG,Kaneko H,Fowler BJ,Bogdanovich S,Albuquerque RJ,Hauswirth WW,Chiodo VA,Kugel JF,Goodrich JA,Ponicsan SL,Chaudhuri G,Murphy MP,Dunaief JL,Ambati BK,Ogura Y

更新日期：2012-05-11 00:00:00

abstract：:We find that inactivation of a Drosophila homolog of the tumor suppressor APC (D-APC) causes retinal neuronal degeneration and pigment cell hypertrophy, a phenotype remarkably similar to that found in humans with germline APC mutations. Retinal degeneration in the D-APC mutant results from apoptotic cell death, which ...

journal_title：Cell

authors： Ahmed Y,Hayashi S,Levine A,Wieschaus E

更新日期：1998-06-26 00:00:00

abstract：:The HIV auxiliary protein Vpr potently blocks the cell cycle at the G2/M transition. Here, we show that G2/M arrest results from untimely activation of the structure-specific endonuclease (SSE) regulator SLX4 complex (SLX4com) by Vpr, a process that requires VPRBP-DDB1-CUL4 E3-ligase complex. Direct interaction of Vpr...

journal_title：Cell

authors： Laguette N,Brégnard C,Hue P,Basbous J,Yatim A,Larroque M,Kirchhoff F,Constantinou A,Sobhian B,Benkirane M

更新日期：2014-01-16 00:00:00

abstract：:22q11.2 microdeletions result in specific cognitive deficits and schizophrenia. Analysis of Df(16)A(+/-) mice, which model this microdeletion, revealed abnormalities in the formation of neuronal dendrites and spines, as well as altered brain microRNAs. Here, we show a drastic reduction of miR-185, which resides within...

journal_title：Cell

authors： Xu B,Hsu PK,Stark KL,Karayiorgou M,Gogos JA

更新日期：2013-01-17 00:00:00

abstract：:Posttranslational modifications of histones have been strongly correlated with transcriptional regulation. In this issue of Cell, comprehensively examined the nature of arginine methyltransferases and histone modifications in p53-mediated transcription. ...

journal_title：Cell

authors： Fingerman IM,Briggs SD

更新日期：2004-06-11 00:00:00

abstract：:Subcellular compartments in which folding and assembly of proteins occur seem to have a set of PCB proteins capable of mediating these and related processes, such as translocation across membranes. When a domain of a polypeptide chain emerges from a ribosome during synthesis or from the distal side of a membrane durin...

journal_title：Cell

authors： Rothman JE

更新日期：1989-11-17 00:00:00

abstract：:Screens for agents that specifically kill epithelial cancer stem cells (CSCs) have not been possible due to the rarity of these cells within tumor cell populations and their relative instability in culture. We describe here an approach to screening for agents with epithelial CSC-specific toxicity. We implemented this ...

journal_title：Cell

authors： Gupta PB,Onder TT,Jiang G,Tao K,Kuperwasser C,Weinberg RA,Lander ES

更新日期：2009-08-21 00:00:00

abstract：:We describe a monoclonal antibody directed against a neuron-specific mitochondrial protein from rat brain. On protein blots the antibody recognizes a single polypeptide of apparent molecular weight of 23,000. By solid-phase immunoassay the antigen was detected in nonneural tissues. Within neurons, the antibody stains ...

journal_title：Cell

authors： Hawkes R,Niday E,Matus A

更新日期：1982-02-01 00:00:00

abstract：:Short DNA chains, isolated from in vitro pulse-labeled replicating polyoma DNA, exhibit some degree of self-complementarity (28% resistance to S1 nuclease after self-annealing to plateau levels). This level of self-annealing is not increased if short DNA chains present as free single-stranded DNA after extraction are ...

journal_title：Cell

authors： Francke B,Vogt M

更新日期：1975-06-01 00:00:00

abstract：:Escherichia coli and Micrococcus luteus DNA topoisomerase I are found to promote catenation of double-stranded DNA rings. At low DNA concentration dimeric catenanes are the major catenated products; at high DNA concentration or when spermidine is present, catenanes containing more than two rings are formed. There is n...

journal_title：Cell

authors： Tse Y,Wang JC

更新日期：1980-11-01 00:00:00

abstract：:Using cryo-electron microscopy, we have solved the structure of an icosidodecahedral COPII coat involved in cargo export from the endoplasmic reticulum (ER) coassembled from purified cargo adaptor Sec23-24 and Sec13-31 lattice-forming complexes. The coat structure shows a tetrameric assembly of the Sec23-24 adaptor la...

journal_title：Cell

authors： Stagg SM,LaPointe P,Razvi A,Gürkan C,Potter CS,Carragher B,Balch WE

更新日期：2008-08-08 00:00:00

abstract：:The sigma factor sigma 70 of E. coli RNA polymerase acts not only in initiation, but also at an early stage of elongation to induce a transcription pause, and simultaneously to allow the phage lambda gene Q transcription antiterminator to act. We identify the signal in DNA that induces early pausing to be a version of...

journal_title：Cell

authors： Ring BZ,Yarnell WS,Roberts JW

更新日期：1996-08-09 00:00:00

abstract：:Activation of the cyclin-dependent kinases to promote cell cycle progression requires their association with cyclins as well as phosphorylation of a threonine (residue 161 in human p34cdc2). This phosphorylation is carried out by CAK, the Cdk-activating kinase. We have purified and cloned CAK from S. cerevisiae. Unlik...

journal_title：Cell

authors： Kaldis P,Sutton A,Solomon MJ

更新日期：1996-08-23 00:00:00

abstract：:Sirtuins are thought to form crucial links between energy levels and cellular metabolism. Libert et al. now provide evidence that SIRT1 activity in the brain modifies mammalian emotional behavior via monoamine signaling and that changes in this pathway might contribute to human affective disorders. ...

journal_title：Cell

authors： Drew LJ,Hen R

更新日期：2011-12-23 00:00:00

abstract：:Plasma membrane vesicles and protoplasts of Escherichia coli were fused to planar lipid bilayers and studied with electrophysiological techniques. Large transmembrane aqueous channels were opened when 0.2 nM LamB signal peptide was added to the cytoplasmic side of the membrane. These aqueous pores are similar in condu...

journal_title：Cell

authors： Simon SM,Blobel G

更新日期：1992-05-15 00:00:00

abstract：:Trigger factor (TF) is a molecular chaperone that binds to bacterial ribosomes where it contacts emerging nascent chains, but TF is also abundant free in the cytosol where its activity is less well characterized. In vitro studies show that TF promotes protein refolding. We find here that ribosome-free TF stably associ...

journal_title：Cell

authors： Martinez-Hackert E,Hendrickson WA

更新日期：2009-09-04 00:00:00

abstract：:Long chain fatty acids (LCFAs) are an important energy substrate used by cardiac myocytes and other cells, but the mechanism whereby these molecules cross the plasma membrane is poorly understood. We used an expression cloning strategy and a cDNA library from 3T3-L1 adipocytes to identify a cDNA that, when expressed i...

journal_title：Cell

authors： Schaffer JE,Lodish HF

更新日期：1994-11-04 00:00:00

abstract：:We studied H1 gene expression in hybrids of Xenopus laevis (female) x Xenopus borealis (male) and saw paternal H1 synthesis in mid-blastulae, which indicates that the H1 genes are active by this stage. The behavior of the maternal store of H1 histone mRNA was studied in androgenetic haploids. In which all stored mRNA ...

journal_title：Cell

authors： Woodland HR,Flynn JM,Wyllie AJ

更新日期：1979-09-01 00:00:00