Abstract:
:Activation of the cyclin-dependent kinases to promote cell cycle progression requires their association with cyclins as well as phosphorylation of a threonine (residue 161 in human p34cdc2). This phosphorylation is carried out by CAK, the Cdk-activating kinase. We have purified and cloned CAK from S. cerevisiae. Unlike CAKs from other organisms, Cak1p is active as a monomer, has full activity when expressed in E. coli, and is not a component of the basal transcription factor, TFIIH. A temperature-sensitive mutation in CAK1 confers a G2 delay accompanied by low Cdc28p protein kinase activity and shows genetic interactions with altered expression of the gene for the major mitotic cyclin, CLB2. Our data raise the intriguing possibility that p40MO15-cyclin H-MAT1, identified as the predominant CAK in vertebrate cell extracts, may not function as a physiological CAK.
journal_name
Celljournal_title
Cellauthors
Kaldis P,Sutton A,Solomon MJdoi
10.1016/s0092-8674(00)80129-4subject
Has Abstractpub_date
1996-08-23 00:00:00pages
553-64issue
4eissn
0092-8674issn
1097-4172pii
S0092-8674(00)80129-4journal_volume
86pub_type
杂志文章相关文献
CELL文献大全abstract::Mucus production by goblet cells of the large intestine serves as a crucial antimicrobial protective mechanism at the interface between the eukaryotic and prokaryotic cells of the mammalian intestinal ecosystem. However, the regulatory pathways involved in goblet cell-induced mucus secretion remain largely unknown. He...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.01.026
更新日期:2014-02-27 00:00:00
abstract::The sequences corresponding to the 18S and 28S rRNAs have been mapped within a cloned 17 kilobase (kb) fragment formed by Eco R1 cleavage of Drosophila melanogaster rDNA. This fragment, Dm103, represents the longer of two major types of repeating units that are present in the rDNA of this fly, and was cloned as a hybr...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(77)90212-4
更新日期:1977-02-01 00:00:00
abstract::Fragile X syndrome, a common form of inherited intellectual disability, is caused by loss of the fragile X mental retardation protein FMRP. FMRP is present predominantly in the cytoplasm, where it regulates translation of proteins that are important for synaptic function. We identify FMRP as a chromatin-binding protei...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.03.040
更新日期:2014-05-08 00:00:00
abstract::Subsets of long-lived, tumor-initiating stem cells often escape cancer therapies. However, sources and mechanisms that generate tumor heterogeneity and drug-resistant cell population are still unfolding. Here, we devise a functional reporter system to lineage trace and/or genetic ablate signaling in TGF-β-activated sq...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.01.043
更新日期:2015-02-26 00:00:00
abstract::Partially edited mRNAs were selected by the polymerase chain reaction and sequenced. In the case of cytochrome b, 102 out of 106 clones displayed patterns of editing that were consistent with a strictly progressive 3' to 5' editing process, as predicted by the guide RNA model of RNA editing. In the case of cytochrome ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90197-m
更新日期:1990-06-01 00:00:00
abstract::The initiation of chromosome segregation at anaphase is linked by the spindle assembly checkpoint to the completion of chromosome-microtubule attachment during metaphase. To determine the function of the mitotic checkpoint protein Mad2 during normal cell division and when mitosis goes awry, we have knocked out Mad2 in...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80875-2
更新日期:2000-06-09 00:00:00
abstract::The synthesis and secretion of plasminogen activator by cultured macrophages can be induced and stimulated by concanavalin A and by phorbol myristate acetate, and inhibited by such agents as glucocorticoids, mitotic inhibitors and compounds affecting cAMP metabolism. By the manipulation of stimulatory and inhibitory i...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(77)90086-1
更新日期:1977-07-01 00:00:00
abstract::On each side of the midline of the Drosophila CNS, axons are organized into a series of parallel pathways. Here we show that the midline repellent Slit, previously identified as a short-range signal that regulates midline crossing, also functions at long range to pattern these longitudinal pathways. In this long-range...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)00207-5
更新日期:2000-12-22 00:00:00
abstract::The sphingomyelin pathway, which is initiated by sphingomyelin hydrolysis to generate the second messenger ceramide, signals apoptosis for tumor necrosis factor alpha, Fas, and ionizing radiation. In the present studies, the anticancer drug daunorubicin also stimulated ceramide elevation and apoptosis in P388 and U937...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(95)90429-8
更新日期:1995-08-11 00:00:00
abstract::The small ribonucleoproteins recognized by anti-La autoantibodies contain a heterogeneous mixture of small RNAs from uninfected mammalian cells. The identity of many of these has now been established by the discovery of precursor forms of 5S rRNA and of certain tRNAs among La RNAs from HeLa cells. The small fraction o...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90099-x
更新日期:1982-05-01 00:00:00
abstract::Stem cells are highly resistant to viral infection compared to their differentiated progeny; however, the mechanism is mysterious. Here, we analyzed gene expression in mammalian stem cells and cells at various stages of differentiation. We find that, conserved across species, stem cells express a subset of genes previ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.11.018
更新日期:2018-01-25 00:00:00
abstract::The 75 kDa tumor necrosis factor receptor (TNFR2) transduces extracellular signals via receptor-associated cytoplasmic proteins. Two of these signal transducers, TRAF1 and TRAF2, were isolated and characterized previously. We report here the biochemical purification and subsequent molecular cloning of two novel TNFR2-...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(95)90149-3
更新日期:1995-12-29 00:00:00
abstract::Kinetochores direct attachment of chromosomes to microtubules of the mitotic spindle during cell division. Three recent studies in Cell, including one in this issue, reveal important new roles for two kinetochore protein complexes-Ndc80 and INCENP-Survivin-in establishing the correct attachment of chromosomes to spind...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2006.11.032
更新日期:2006-12-15 00:00:00
abstract::In vertebrates, muscles of the limbs and body wall derive from the lateral compartment of the embryonic somites, and axial muscles derive from the medial compartment. Whereas the mechanisms that direct patterning of somites along the dorsoventral axis are beginning to be understood, little is known about the tissue in...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81291-x
更新日期:1996-02-09 00:00:00
abstract::The subcellular distribution of the type II enzyme of cAMP-dependent protein kinase (cAMP-dPK II) in epithelial and fibroblastic cells was determined by indirect immunofluorescence microscopy. In interphase cells both regulatory (R II) and catalytic (C) subunits were concentrated in a perinuclear area. By comparison o...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(85)80084-2
更新日期:1985-07-01 00:00:00
abstract::Screens for agents that specifically kill epithelial cancer stem cells (CSCs) have not been possible due to the rarity of these cells within tumor cell populations and their relative instability in culture. We describe here an approach to screening for agents with epithelial CSC-specific toxicity. We implemented this ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.06.034
更新日期:2009-08-21 00:00:00
abstract::Double-strand break (DSB) repair is critical for maintaining genomic integrity and requires the processing of the 5' DSB ends. Recent studies have shed light on the mechanism and regulation of DNA end processing during DSB repair by homologous recombination. ...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2009.05.007
更新日期:2009-05-29 00:00:00
abstract::Suppressor of Hairless [Su(H)]/Lag-1/RBP-Jkappa/CBF1 is the only known transducing transcription factor for Notch receptor signaling. Here, we show that Su(H) has three distinct functions in the development of external mechanosensory organs in Drosophila: Notch-dependent transcriptional activation and a novel auto-rep...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)00198-7
更新日期:2000-12-08 00:00:00
abstract::Enveloped viruses enter cells by inducing fusion of viral and cellular membranes, a process catalyzed by a specialized membrane-fusion protein expressed on their surface. This review focuses on recent structural studies of viral fusion proteins with an emphasis on their metastable prefusion form and on interactions wi...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2018.02.054
更新日期:2018-03-08 00:00:00
abstract::The liver is capable of completely regenerating itself in response to injury and after partial hepatectomy. In liver of old animals, the proliferative response is dramatically reduced, the mechanism for which is unknown. The liver specific protein, C/EBPalpha, normally arrests proliferation of hepatocytes through inhi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(03)00318-0
更新日期:2003-05-16 00:00:00
abstract::Although a great deal is known biochemically about peroxiredoxins (Prxs), little is known about their real physiological function. We show here that two cytosolic yeast Prxs, cPrxI and II, which display diversity in structure and apparent molecular weights (MW), can act alternatively as peroxidases and molecular chape...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2004.05.002
更新日期:2004-05-28 00:00:00
abstract::Characterizing cell surface receptors mediating viral infection is critical for understanding viral tropism and developing antiviral therapies. Nevertheless, due to challenges associated with detecting protein interactions on the cell surface, the host receptors of many human pathogens remain unknown. Here, we build a...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.06.028
更新日期:2018-08-23 00:00:00
abstract::During development, neurons switch among growth states, such as initial axon outgrowth, axon pruning, and regrowth. By studying the stereotypic remodeling of the Drosophila mushroom body (MB), we found that the heme-binding nuclear receptor E75 is dispensable for initial axon outgrowth of MB γ neurons but is required ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.11.047
更新日期:2016-01-14 00:00:00
abstract::We have determined the nucleotide sequence of a secondary lambda attachment site in trpC. Direct sequence analysis of lambdatrp transducing phage DNA fragments carrying the two prophage attachment sites reveals a 6 nucleotide homology in the crossover region which is a subset of the 15 nucleotide core sequence in the ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90016-3
更新日期:1979-02-01 00:00:00
abstract::We have isolated and characterized a set of overlapping cDNA clones that encode the human centromere autoantigen centromere protein C (CENP-C). The identity of these clones has been established using several criteria. First, they were shown to encode a polypeptide that migrates at the expected position for CENP-C on S...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90538-n
更新日期:1992-07-10 00:00:00
abstract::The alpha IIb beta 3 integrin binds Arg-Gly-Asp-containing (RGD-containing) ligands in a cation-dependent interaction. A fourteen amino acid sequence, beta 3 (118-131), and an antibody to it, inhibited ligand binding functions of alpha IIb beta 3, and a 1:1 stoichiometric beta 3 (118-131)-RGD complex was detected by m...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90551-7
更新日期:1994-11-18 00:00:00
abstract::Targeted gene regulation on a genome-wide scale is a powerful strategy for interrogating, perturbing, and engineering cellular systems. Here, we develop a method for controlling gene expression based on Cas9, an RNA-guided DNA endonuclease from a type II CRISPR system. We show that a catalytically dead Cas9 lacking en...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.02.022
更新日期:2013-02-28 00:00:00
abstract::We have shown that the DNA sequences adjacent to the telomeres of Saccharomyces cerevisiae chromosomes are highly conserved and contain a high density of replication origins. The salient features of these telomeres can be summarized as follows. There are three moderately repetitive elements present at the telomeres: t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90437-3
更新日期:1983-06-01 00:00:00
abstract::Immune responses in diverse tissue sites are critical for protective immunity and homeostasis. Here, we investigate how tissue localization regulates the development and function of human natural killer (NK) cells, innate lymphocytes important for anti-viral and tumor immunity. Integrating high-dimensional analysis of...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.01.022
更新日期:2020-02-20 00:00:00
abstract::Eukaryotic Argonaute proteins strictly mediate RNA-guided RNA interference. In contrast, prokaryotic Argonautes can utilize DNA guides to target complementary DNA sequences to protect their hosts against invading DNA. In this issue of Cell, Jolly and colleagues demonstrate that Thermus thermophilus Argonaute additiona...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.08.037
更新日期:2020-09-17 00:00:00