The TNFR2-TRAF signaling complex contains two novel proteins related to baculoviral inhibitor of apoptosis proteins.


:The 75 kDa tumor necrosis factor receptor (TNFR2) transduces extracellular signals via receptor-associated cytoplasmic proteins. Two of these signal transducers, TRAF1 and TRAF2, were isolated and characterized previously. We report here the biochemical purification and subsequent molecular cloning of two novel TNFR2-associated proteins, designated c-IAP1 and c-IAP2, that are closely related mammalian members of the inhibitor of apoptosis protein (IAP) family originally identified in baculoviruses. The viral and cellular IAPs contain N-terminal baculovirus IAP repeat (BIR) motifs and a C-terminal RING finger. The c-IAPs do not directly contact TNFR2, but rather associate with TRAF1 and TRAF2 through their N-terminal BIR motif-comprising domain. The recruitment of c-IAP1 or c-IAP2 to the TNFR2 signaling complex requires a TRAF2-TRAF1 heterocomplex.






Rothe M,Pan MG,Henzel WJ,Ayres TM,Goeddel DV




Has Abstract


1995-12-29 00:00:00
















  • Specific and saturable binding of pp60v-src to plasma membranes: evidence for a myristyl-src receptor.

    abstract::The molecular basis for membrane association of pp60v-src, the transforming protein of Rous sarcoma virus, was investigated in a cell-free system. Newly synthesized pp60v-src polypeptide, produced by in vitro translation of src mRNA, rapidly bound to plasma membranes. Binding was saturable and dependent on the presenc...


    pub_type: 杂志文章


    authors: Resh MD

    更新日期:1989-07-28 00:00:00

  • p53-dependent inhibition of cyclin-dependent kinase activities in human fibroblasts during radiation-induced G1 arrest.

    abstract::gamma-Irradiation of human diploid fibroblasts in the G1 interval caused arrest of the cell cycle prior to S phase. This cell cycle block was correlated with a lack of activation of both cyclin E-Cyclin-dependent kinase 2 (Cdk2) and cyclin A-Cdk2 kinases and depended on wild-type p53. Although the accumulation of cycl...


    pub_type: 杂志文章


    authors: Dulić V,Kaufmann WK,Wilson SJ,Tlsty TD,Lees E,Harper JW,Elledge SJ,Reed SI

    更新日期:1994-03-25 00:00:00

  • The mouse segmentation gene kr encodes a novel basic domain-leucine zipper transcription factor.

    abstract::The mouse kreisler (kr) mutation causes segmentation abnormalities in the caudal hindbrain and defective inner ear development. Based on an inversion discovered in the original kr allele, we selected a candidate cDNA highly expressed in the developing caudal hindbrain. This cDNA encodes a basic domain-leucine zipper (...


    pub_type: 杂志文章


    authors: Cordes SP,Barsh GS

    更新日期:1994-12-16 00:00:00

  • Crystal structure of the complex of human epidermal growth factor and receptor extracellular domains.

    abstract::Epidermal growth factor (EGF) regulates cell proliferation and differentiation by binding to the EGF receptor (EGFR) extracellular region, comprising domains I-IV, with the resultant dimerization of the receptor tyrosine kinase. In this study, the crystal structure of a 2:2 complex of human EGF and the EGFR extracellu...


    pub_type: 杂志文章


    authors: Ogiso H,Ishitani R,Nureki O,Fukai S,Yamanaka M,Kim JH,Saito K,Sakamoto A,Inoue M,Shirouzu M,Yokoyama S

    更新日期:2002-09-20 00:00:00

  • Vacuole fusion at a ring of vertex docking sites leaves membrane fragments within the organelle.

    abstract::Three membrane microdomains can be identified on docked vacuoles: "outside" membrane, not in contact with other vacuoles, "boundary" membrane that contacts adjacent vacuoles, and "vertices," where boundary and outside membrane meet. In living cells and in vitro, vacuole fusion occurs at vertices rather than from a cen...


    pub_type: 杂志文章


    authors: Wang L,Seeley ES,Wickner W,Merz AJ

    更新日期:2002-02-08 00:00:00

  • Insulin Receptor Associates with Promoters Genome-wide and Regulates Gene Expression.

    abstract::Insulin receptor (IR) signaling is central to normal metabolic control and dysregulated in prevalent chronic diseases. IR binds insulin at the cell surface and transduces rapid signaling via cytoplasmic kinases. However, mechanisms mediating long-term effects of insulin remain unclear. Here, we show that IR associates...


    pub_type: 杂志文章


    authors: Hancock ML,Meyer RC,Mistry M,Khetani RS,Wagschal A,Shin T,Ho Sui SJ,Näär AM,Flanagan JG

    更新日期:2019-04-18 00:00:00

  • Single-Cell Analysis: The Differences That Kill.

    abstract::Using single-cell RNA sequencing, Avraham et al. investigate how variability in macrophage response to infection is controlled by variability within the pathogen population. They find that heterogeneous expression of the Salmonella virulence factor PhoP and subsequent cell-wall modifications lead to the bimodal induct...


    pub_type: 评论,杂志文章


    authors: Tay S

    更新日期:2015-09-10 00:00:00

  • RNA Sensing by Gut Piezo1 Is Essential for Systemic Serotonin Synthesis.

    abstract::Gastrointestinal enterochromaffin cells regulate bone and gut homeostasis via serotonin (5-hydroxytryptamine [5-HT]) production. A recent report suggested that gut microbes regulate 5-HT levels; however, the precise underlying molecular mechanisms are unexplored. Here, we reveal that the cation channel Piezo1 in the g...


    pub_type: 杂志文章


    authors: Sugisawa E,Takayama Y,Takemura N,Kondo T,Hatakeyama S,Kumagai Y,Sunagawa M,Tominaga M,Maruyama K

    更新日期:2020-08-06 00:00:00

  • It takes a PHD to read the histone code.

    abstract::The pattern of histone modifications, called the histone code, influences transitions between chromatin states and the regulation of transcriptional activity. Four recent papers describe how plant homeodomain (PHD) finger proteins read part of this code. The PHD finger may promote both gene expression and repression t...


    pub_type: 杂志文章,评审


    authors: Mellor J

    更新日期:2006-07-14 00:00:00

  • Inactivation of the mouse Msh2 gene results in mismatch repair deficiency, methylation tolerance, hyperrecombination, and predisposition to cancer.

    abstract::To investigate the role of the presumed DNA mismatch repair (MMR) gene Msh2 in genome stability and tumorigenesis, we have generated cells and mice that are deficient for the gene. Msh2-deficient cells have lost mismatch binding and have acquired microsatellite instability, a mutator phenotype, and tolerance to methyl...


    pub_type: 杂志文章


    authors: de Wind N,Dekker M,Berns A,Radman M,te Riele H

    更新日期:1995-07-28 00:00:00

  • Little effect of the tan locus on pigmentation in female hybrids between Drosophila santomea and D. melanogaster.

    abstract::Previous work on Drosophila santomea suggested that its absence of abdominal pigmentation, compared to the other darkly pigmented species, is based on mutations in the cis-regulatory region of tan, inactivating the expression of that gene in the abdomen of D. santomea males and females. Our discovery that D. santomea ...


    pub_type: 杂志文章


    authors: Matute DR,Butler IA,Coyne JA

    更新日期:2009-12-11 00:00:00

  • Cancer-associated protein kinase C mutations reveal kinase's role as tumor suppressor.

    abstract::Protein kinase C (PKC) isozymes have remained elusive cancer targets despite the unambiguous tumor promoting function of their potent ligands, phorbol esters, and the prevalence of their mutations. We analyzed 8% of PKC mutations identified in human cancers and found that, surprisingly, most were loss of function and ...


    pub_type: 杂志文章


    authors: Antal CE,Hudson AM,Kang E,Zanca C,Wirth C,Stephenson NL,Trotter EW,Gallegos LL,Miller CJ,Furnari FB,Hunter T,Brognard J,Newton AC

    更新日期:2015-01-29 00:00:00

  • Biotech's perfect storm.

    abstract::The global financial crisis has hit biotech companies hard both in the US and Europe as venture capital dries up. Finding new sources of long-term financing for translating research into new therapeutics will be essential for maintaining innovation and new drug development by biotech companies. ...


    pub_type: 杂志文章


    authors: Karberg S

    更新日期:2009-08-07 00:00:00

  • Mosaic analysis with double markers reveals tumor cell of origin in glioma.

    abstract::Cancer cell of origin is difficult to identify by analyzing cells within terminal stage tumors, whose identity could be concealed by the acquired plasticity. Thus, an ideal approach to identify the cell of origin is to analyze proliferative abnormalities in distinct lineages prior to malignancy. Here, we use mosaic an...


    pub_type: 杂志文章


    authors: Liu C,Sage JC,Miller MR,Verhaak RG,Hippenmeyer S,Vogel H,Foreman O,Bronson RT,Nishiyama A,Luo L,Zong H

    更新日期:2011-07-22 00:00:00

  • Determination of the amount of homology required for recombination in bacteriophage T4.

    abstract::Homology is an important feature of recombination. We have used the rll cistrons of bacteriophage T4 to determine the extent of homology required for recombination. We varied the amount of homologous DNA available for recombination in both marker rescue experiments and deletion-by-deletion crosses. Our results suggest...


    pub_type: 杂志文章


    authors: Singer BS,Gold L,Gauss P,Doherty DH

    更新日期:1982-11-01 00:00:00

  • The 5' terminal structure of the methylated mRNA synthesized in vitro by vesicular stomatitis virus.

    abstract::The 5' terminal structure of the mRNA synthesized in vitro by the virion-associated RNA polymerase of vesicular stomatitis virus in the presence of S-adenosyl-L-methione consists of 7-methyl guanosine linked to 2'-O-methyl adenosine through a 5'-5' pyrophosphate bond as m7G(5')ppp(5')A-m-p ... The alpha and beta phosp...


    pub_type: 杂志文章


    authors: Abraham G,Rhodes DP,Banerjee AK

    更新日期:1975-05-01 00:00:00

  • Suppression of PKR promotes network excitability and enhanced cognition by interferon-γ-mediated disinhibition.

    abstract::The double-stranded RNA-activated protein kinase (PKR) was originally identified as a sensor of virus infection, but its function in the brain remains unknown. Here, we report that the lack of PKR enhances learning and memory in several behavioral tasks while increasing network excitability. In addition, loss of PKR i...


    pub_type: 杂志文章


    authors: Zhu PJ,Huang W,Kalikulov D,Yoo JW,Placzek AN,Stoica L,Zhou H,Bell JC,Friedlander MJ,Krnjević K,Noebels JL,Costa-Mattioli M

    更新日期:2011-12-09 00:00:00

  • Translational control by MAPK signaling in long-term synaptic plasticity and memory.

    abstract::Enduring forms of synaptic plasticity and memory require new protein synthesis, but little is known about the underlying regulatory mechanisms. Here, we investigate the role of MAPK signaling in these processes. Conditional expression of a dominant-negative form of MEK1 in the postnatal murine forebrain inhibited ERK ...


    pub_type: 杂志文章


    authors: Kelleher RJ 3rd,Govindarajan A,Jung HY,Kang H,Tonegawa S

    更新日期:2004-02-06 00:00:00

  • The president's scientist.

    abstract::The science advisor to the US president has the opportunity to influence how scientific research is supported by the federal government, as well as the ways in which science and technology can be applied to meet the needs of society. ...


    pub_type: 杂志文章


    authors: Lane NF,Matthews KR

    更新日期:2009-11-25 00:00:00

  • Vesicle-mediated export and assembly of pore-forming oligomers of the enterobacterial ClyA cytotoxin.

    abstract::The ClyA protein is a pore-forming cytotoxin expressed by Escherichia coli and some other enterobacteria. It confers cytotoxic activity toward mammalian cells, but it has remained unknown how ClyA is surface exposed and exported from bacterial cells. Outer-membrane vesicles (OMVs) released from the bacteria were shown...


    pub_type: 杂志文章


    authors: Wai SN,Lindmark B,Söderblom T,Takade A,Westermark M,Oscarsson J,Jass J,Richter-Dahlfors A,Mizunoe Y,Uhlin BE

    更新日期:2003-10-03 00:00:00

  • Function of retinoic acid receptor gamma in the mouse.

    abstract::Null mutant mice for retinoic acid receptor gamma 2 (RAR gamma 2) or all RAR gamma isoforms were generated. RAR gamma 2 mutants appeared normal, whereas RAR gamma mutants exhibited growth deficiency, early lethality, and male sterility due to squamous metaplasia of the seminal vesicles and prostate. These defects were...


    pub_type: 杂志文章


    authors: Lohnes D,Kastner P,Dierich A,Mark M,LeMeur M,Chambon P

    更新日期:1993-05-21 00:00:00

  • Structural basis for the function of the ribosomal L7/12 stalk in factor binding and GTPase activation.

    abstract::The L7/12 stalk of the large subunit of bacterial ribosomes encompasses protein L10 and multiple copies of L7/12. We present crystal structures of Thermotoga maritima L10 in complex with three L7/12 N-terminal-domain dimers, refine the structure of an archaeal L10E N-terminal domain on the 50S subunit, and identify th...


    pub_type: 杂志文章


    authors: Diaconu M,Kothe U,Schlünzen F,Fischer N,Harms JM,Tonevitsky AG,Stark H,Rodnina MV,Wahl MC

    更新日期:2005-07-01 00:00:00

  • FGF21 and the second coming of PPARγ.

    abstract::Peptide hormone fibroblast growth factor-21 (FGF21) has insulin-mimetic properties. Dutchak et al. now suggest that FGF21 also acts in an autocrine fashion in adipocytes and is required to mediate effects of the PPARγ agonist class of antidiabetic drugs. Does this new property improve FGF21's fledgling clinical prospe...


    pub_type: 评论,杂志文章


    authors: Qiang L,Accili D

    更新日期:2012-02-03 00:00:00

  • Phosphorylation of intermediate filament proteins by cAMP-dependent protein kinases.

    abstract::The intermediate filament proteins, desmin and vimentin, are phosphorylated in skeletal muscle cells in vivo. Desmin kinase activities have been purified from mature chicken skeletal muscle and identified as the cAMP-dependent kinases. Chicken skeletal muscle contains two cAMP-dependent protein kinases which are simil...


    pub_type: 杂志文章


    authors: O'Connor CM,Gard DL,Lazarides E

    更新日期:1981-01-01 00:00:00

  • MIX-1: an essential component of the C. elegans mitotic machinery executes X chromosome dosage compensation.

    abstract::We show that a functional component of the C. elegans mitotic machinery regulates X chromosome gene expression. This protein, MIX-1, is a member of the dosage compensation complex that associates specifically with hermaphrodite X chromosomes to reduce their gene expression during interphase. MIX-1 also associates with...


    pub_type: 杂志文章


    authors: Lieb JD,Albrecht MR,Chuang PT,Meyer BJ

    更新日期:1998-01-23 00:00:00

  • The nucleotide sequence of the maize controlling element Activator.

    abstract::We have determined the nucleotide sequence of the transposable maize controlling element Activator (Ac). The Ac element is 4563 bp long and has an imperfect terminal repetition of 11 bp. The element contains two open reading frames (ORF) encoding polypeptides of 839 and 210 amino acids. Evidence derived from structura...


    pub_type: 杂志文章


    authors: Pohlman RF,Fedoroff NV,Messing J

    更新日期:1984-06-01 00:00:00

  • Gene organization and recombinational hotspots in the murine major histocompatibility complex.

    abstract::By chromosome walking in the major histocompatibility complex (MHC) of the BALB/c mouse, we have linked the K region to the I region at the molecular level. Forty-nine overlapping cosmid clones define a stretch of about 600 kb of DNA containing 2 class I and 7 class II genes. Eleven independent recombination events we...


    pub_type: 杂志文章


    authors: Steinmetz M,Stephan D,Fischer Lindahl K

    更新日期:1986-03-28 00:00:00

  • The Ancient Gamete Fusogen HAP2 Is a Eukaryotic Class II Fusion Protein.

    abstract::Sexual reproduction is almost universal in eukaryotic life and involves the fusion of male and female haploid gametes into a diploid cell. The sperm-restricted single-pass transmembrane protein HAP2-GCS1 has been postulated to function in membrane merger. Its presence in the major eukaryotic taxa-animals, plants, and ...


    pub_type: 杂志文章


    authors: Fédry J,Liu Y,Péhau-Arnaudet G,Pei J,Li W,Tortorici MA,Traincard F,Meola A,Bricogne G,Grishin NV,Snell WJ,Rey FA,Krey T

    更新日期:2017-02-23 00:00:00

  • Extending the host range of Listeria monocytogenes by rational protein design.

    abstract::In causing disease, pathogens outmaneuver host defenses through a dedicated arsenal of virulence determinants that specifically bind or modify individual host molecules. This dedication limits the intruder to a defined range of hosts. Newly emerging diseases mostly involve existing pathogens whose arsenal has been alt...


    pub_type: 杂志文章


    authors: Wollert T,Pasche B,Rochon M,Deppenmeier S,van den Heuvel J,Gruber AD,Heinz DW,Lengeling A,Schubert WD

    更新日期:2007-06-01 00:00:00

  • Separation of drug transport and chloride channel functions of the human multidrug resistance P-glycoprotein.

    abstract::The human multidrug resistance P-glycoprotein is an active transporter that pumps cytotoxic drugs out of cells. Expression of P-glycoprotein is also associated with a volume-activated chloride channel. Here we address the relationship between these two functions. Drug transport requires ATP hydrolysis while, in contra...


    pub_type: 杂志文章


    authors: Gill DR,Hyde SC,Higgins CF,Valverde MA,Mintenig GM,Sepúlveda FV

    更新日期:1992-10-02 00:00:00