Abstract：
:Plasmids expressing partial-length sense or antisense protein 4.1 RNA were microinjected into fertilized Xenopus eggs. Nuclease protection assays reveal that antisense protein 4.1 RNA lead to the specific loss of endogenous protein 4.1 transcripts after midblastula transition, with no effect on the levels of three unrelated transcripts. As a control, we show that this dramatic loss of endogenous protein 4.1 transcripts is blocked when fertilized eggs receive a second injection of plasmids that express partial-length sense protein 4.1 RNA. Immunocytochemistry of tadpole embryos with antibodies monospecific for protein 4.1 demonstrates that the antisense protein 4.1 RNA blocks the normal expression of protein 4.1 in embryos and interferes with the normal interdigitation of the photoreceptor outer segments with the pigment epithelium layer in the retina. These data suggest that reduced expression of a single membrane skeleton protein is sufficient to perturb normal cellular interactions of the retina.
journal_name
Celljournal_title
Cellauthors
Giebelhaus DH,Eib DW,Moon RTdoi
10.1016/0092-8674(88)90576-4subject
Has Abstractpub_date
1988-05-20 00:00:00pages
601-15issue
4eissn
0092-8674issn
1097-4172pii
0092-8674(88)90576-4journal_volume
53pub_type
杂志文章相关文献
CELL文献大全abstract：:Transfer of human chromosome 11, which contains the myoD locus, from primary fibroblasts into 10T1/2 cells results in activation of myoD. In contrast, hybrids that retain human chromosome 11 and additional human chromosomes fail to activate myoD. We show that human chromosome 4 inhibits myoD activation. myoD enhancer/...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(95)90033-0
更新日期：1995-08-25 00:00:00
abstract：:Evasion of DNA damage-induced cell death, via mutation of the p53 tumor suppressor or overexpression of prosurvival Bcl-2 family proteins, is a key step toward malignant transformation and therapeutic resistance. We report that depletion or acute inhibition of checkpoint kinase 1 (Chk1) is sufficient to restore gamma-...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2008.03.037
更新日期：2008-05-30 00:00:00
abstract：:The modulation of cell metabolism by cell shape and external surface contact has been studied by suspension culture of anchorage-dependent fibroblasts. The suspended cells shut down protein synthesis and nuclear RNA metabolism and cease replicating DNA. However, these responses to suspension are lost or modified as ce...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90111-2
更新日期：1981-06-01 00:00:00
abstract：:We present evidence that Tn10 transposase promotes double strand breaks and single strand joints at Tn10 termini in vivo. Plasmids containing a shortened Tn10 element and a transposase overproducer fusion give rise, upon transposase induction, to new DNA species. The most prominent class is a circularized transposon m...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(84)90204-6
更新日期：1984-11-01 00:00:00
abstract：:Inward rectifier K+ channels mediate the K+ conductance at resting potential in many types of cell. Since these K+ channels do not pass outward currents (inward rectification) when the cell membrane is depolarized beyond a trigger threshold, they play an important role in controlling excitability. Both a highly voltag...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(95)90459-x
更新日期：1995-01-13 00:00:00
abstract：:The extracellular space (ECS) of the brain has an extremely complex spatial organization, which has defied conventional light microscopy. Consequently, despite a marked interest in the physiological roles of brain ECS, its structure and dynamics remain largely inaccessible for experimenters. We combined 3D-STED micros...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.02.007
更新日期：2018-02-22 00:00:00
abstract：:Neural progenitors generate distinct cell types at different stages, but the mechanisms controlling these temporal transitions are poorly understood. In the Drosophila CNS, a cascade of transcription factors, the "temporal gene cascade," has been identified that acts to alter progenitor competence over time. However, ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2009.10.032
更新日期：2009-11-25 00:00:00
abstract：:The adaptive immune system is important for control of most viral infections. The three fundamental components of the adaptive immune system are B cells (the source of antibodies), CD4+ T cells, and CD8+ T cells. The armamentarium of B cells, CD4+ T cells, and CD8+ T cells has differing roles in different viral infect...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2021.01.007
更新日期：2021-01-12 00:00:00
abstract：:The unfolded protein response (UPR) regulates gene expression in response to stress in the endoplasmic reticulum (ER). We determined the transcriptional scope of the UPR using DNA microarrays. Rather than regulating only ER-resident chaperones and phospholipid biosynthesis, as anticipated from earlier work, the UPR af...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80835-1
更新日期：2000-04-28 00:00:00
abstract：:Gastrulation is a complex process requiring the coordination of cell shape changes and cell movements. In Drosophila, gastrulation begins immediately upon cellularization of the blastoderm stage embryo with the formation of the ventral furrow and posterior midgut. Cells that form both of these invaginations change the...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(91)90652-f
更新日期：1991-01-25 00:00:00
abstract：:SR proteins constitute a family of pre-mRNA splicing factors that play important roles in both constitutive and regulated splicing. Here, we describe one member of the family, which we call SRp38, with unexpected properties. Unlike other SR proteins, SRp38 cannot activate splicing and is essentially inactive in splici...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(02)01038-3
更新日期：2002-11-01 00:00:00
abstract：:Procollagen and collagen were isolated from the culture medium and cell layer of line TSD4 (obtained from mouse teratocarcinoma OTT6050). SDS-polyacrylamide gel electrophoresis of the highly purified procollagen fraction demonstrated that the fraction is composed of theta chains (150,000 daltons), pro alpha chains (13...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(77)90222-7
更新日期：1977-02-01 00:00:00
abstract：:The P7C3 class of aminopropyl carbazole chemicals fosters the survival of neurons in a variety of rodent models of neurodegeneration or nerve cell injury. To uncover its mechanism of action, an active derivative of P7C3 was modified to contain both a benzophenone for photocrosslinking and an alkyne for CLICK chemistry...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.07.040
更新日期：2014-09-11 00:00:00
abstract：:Mitochondrial respiratory-chain complexes assemble from subunits of dual genetic origin assisted by specialized assembly factors. Whereas core subunits are translated on mitochondrial ribosomes, others are imported after cytosolic translation. How imported subunits are ushered to assembly intermediates containing mito...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2012.11.053
更新日期：2012-12-21 00:00:00
abstract：:NPR1 is an essential regulator of plant systemic acquired resistance (SAR), which confers immunity to a broad-spectrum of pathogens. SAR induction results in accumulation of the signal molecule salicylic acid (SA), which induces defense gene expression via activation of NPR1. We found that in an uninduced state, NPR1 ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(03)00429-x
更新日期：2003-06-27 00:00:00
abstract：:We have analyzed the transcriptional regulation of plastid genes during chloroplast development in illuminated spinach cotyledons and during leaf formation. The RNAs encoded by plastid genes accumulate with different kinetics during the developmental transitions. Using a novel plastid run-on transcription assay we dem...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(87)90290-x
更新日期：1987-05-08 00:00:00
abstract：:Recent studies have revealed that multiple intracellular signaling proteins may assemble into structured, yet sometimes infinite, higher-order signaling machines for transmission of receptor activation information to cellular responses. These studies advance our understanding of cell signaling and implicate new molecu...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.03.013
更新日期：2013-04-11 00:00:00
abstract：:A "switch" from oxidative phosphorylation (OXPHOS) to aerobic glycolysis is a hallmark of T cell activation and is thought to be required to meet the metabolic demands of proliferation. However, why proliferating cells adopt this less efficient metabolism, especially in an oxygen-replete environment, remains incomplet...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.05.016
更新日期：2013-06-06 00:00:00
abstract：:During neural tube closure and spinal cord development, many cells die in both the central and peripheral nervous systems (CNS and PNS, respectively). However, myeloid-derived professional phagocytes have not yet colonized the trunk region during early neurogenesis. How apoptotic cells are removed from this region dur...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2019.08.001
更新日期：2019-09-19 00:00:00
abstract：:Xenogeneic spinal cord chimeras were constructed by grafting fragments of quail neural primordium into chick embryos at 2 days of incubation. Hatched birds displayed normal motor behavior for about 5 to 7 weeks, whereupon they developed a neurological syndrome; in the grafted spinal cord the pathological signs of the ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(86)90395-8
更新日期：1986-04-25 00:00:00
abstract：:The alpha IIb beta 3 integrin binds Arg-Gly-Asp-containing (RGD-containing) ligands in a cation-dependent interaction. A fourteen amino acid sequence, beta 3 (118-131), and an antibody to it, inhibited ligand binding functions of alpha IIb beta 3, and a 1:1 stoichiometric beta 3 (118-131)-RGD complex was detected by m...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(94)90551-7
更新日期：1994-11-18 00:00:00
abstract：:Chemotherapy is designed to induce cell death. However, at non-lethal doses, cancer cells can choose to remain proliferative or become senescent. The slow development of senescence makes studying this decision challenging. Here, by analyzing single-cell p21 dynamics before, during, and days after drug treatment, we li...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2019.05.041
更新日期：2019-07-11 00:00:00
abstract：:Planar polarity is a global, tissue-level phenomenon that coordinates cell behavior in a two-dimensional plane. The Frizzled/planar cell polarity (PCP) and anterior-posterior (AP) patterning systems for planar polarity operate in a variety of cell types and provide direction to cells with different morphologies and be...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2007.05.050
更新日期：2007-06-15 00:00:00
abstract：:We have examined the chromatin structure of a repeated cluster of tRNA genes of Xenopus laevis, with particular attention to possible phase relationships between nucleosomes and the underlying tDNA sequence. In transcriptionally active liver and cultured kidney cells, essentially all tDNA is packaged into regularly sp...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90387-1
更新日期：1981-12-01 00:00:00
abstract：:Antigen presenting cells (macrophages and dendritic cells) express pattern recognition molecules that are thought to recognize foreign ligands during early phases of the immune response. The best known of these are probably the Toll-like receptors, but a number of other receptors are also involved. Several of these re...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/s0092-8674(02)01201-1
更新日期：2002-12-27 00:00:00
abstract：:The orderly reduction in chromosome number that occurs during meiosis depends on two aspects of chromosome behavior specific to the first meiotic division. These are the retention of cohesion between sister centromeres and their attachment to microtubules that extend to the same pole (monopolar attachment). By deletin...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)00217-8
更新日期：2000-12-22 00:00:00
abstract：:The c-Abl tyrosine kinase is inhibited by mechanisms that are poorly understood. Disruption of these mechanisms in the Bcr-Abl oncoprotein leads to several forms of human leukemia. We found that like Src kinases, c-Abl 1b is activated by phosphotyrosine ligands. Ligand-activated c-Abl is particularly sensitive to the ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(03)00191-0
更新日期：2003-03-21 00:00:00
abstract：:Muscle atrophy (cachexia) in cancer patients is a life-threatening condition for which therapeutic options are limited. Zhou et al. (2010) now identify a new target for treating cachexia, the activin type-2 receptor (ActRIIB). In several mouse models of cachexia, the authors reversed wasting of skeletal and cardiac mu...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2010.08.004
更新日期：2010-08-20 00:00:00
abstract：:We report the identification and characterization of a class of IS10 transposase mutants that carry out only some of the steps required for transposition. These mutants were identified among transposition-defective mutants as a specific subclass that retains the wild-type ability to induce SOS functions in the presenc...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(89)90299-7
更新日期：1989-10-20 00:00:00
abstract：:The crystal structure of the snake toxin fasciculin, bound to mouse acetylcholinesterase (mAChE), at 3.2 A resolution reveals a synergistic three-point anchorage consistent with the picomolar dissociation constant of the complex. Loop II of fasciculin contains a cluster of hydrophobic residues that interact with the p...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(95)90128-0
更新日期：1995-11-03 00:00:00
