Abstract:
:The axonal shafts of neurons contain bundled microtubules, whereas extending growth cones contain unbundled microtubule filaments, suggesting that localized activation of microtubule-associated proteins (MAP) at the transition zone may bundle these filaments during axonal growth. Dephosphorylation is thought to lead to MAP activation, but specific molecular pathways have remained elusive. We find that Spinophilin, a Protein-phosphatase 1 (PP1) targeting protein, is responsible for the dephosphorylation of the MAP Doublecortin (Dcx) Ser 297 selectively at the "wrist" of growing axons, leading to activation. Loss of activity at the "wrist" is evident as an impaired microtubule cytoskeleton along the shaft. These findings suggest that spatially restricted adaptor-specific MAP reactivation through dephosphorylation is important in organization of the neuronal cytoskeleton.
journal_name
Celljournal_title
Cellauthors
Bielas SL,Serneo FF,Chechlacz M,Deerinck TJ,Perkins GA,Allen PB,Ellisman MH,Gleeson JGdoi
10.1016/j.cell.2007.03.023subject
Has Abstractpub_date
2007-05-04 00:00:00pages
579-91issue
3eissn
0092-8674issn
1097-4172pii
S0092-8674(07)00389-3journal_volume
129pub_type
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