Abstract:
:The linear extrachromosomal ribosomal DNA of Tetrahymena is generated from a single integrated copy during macronuclear development. The free ends of this extrachromosomal gene contain 20-70 tandem repeats of the hexanucleotide CCCCAA. We have determined the nucleotide sequence at the same (3') end of the single, integrated micronuclear gene. In contrast to the extrachromosomal gene, only a single CCCCAA sequence was found at this position. The same result was obtained from two independently isolated DNA clones, and was therefore not likely an artifact of cloning. Comparisons of the genomic DNA with the cloned fragment by Southern hybridization also supported this argument. Thus the tandemly repetitive hexanucleotide at the free ends of the extrachromosomal rDNA is not an inherited feature, and must be generated during the development of the macronucleus.
journal_name
Celljournal_title
Cellauthors
King BO,Yao MCdoi
10.1016/0092-8674(82)90417-2subject
Has Abstractpub_date
1982-11-01 00:00:00pages
177-82issue
1eissn
0092-8674issn
1097-4172pii
0092-8674(82)90417-2journal_volume
31pub_type
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