Abstract:
:Fractionation of a microsomal detergent extract with ammonium sulfate allows separation of the signal recognition particle receptor (SR alpha), which is required for targeting of the nascent chain, from other microsomal proteins, such as signal peptidase, whose activity is displayed during subsequent translocation. The reconstituted SR alpha-enriched fraction is functional in assays of precursor targeting and elongation arrest release but lacks translocation activity. This defect can be complemented by addition, prior to reconstitution, of a separate protein subfraction. In addition, protein components necessary for translocation can be reversibly depleted from the complementary fraction, under conditions where precursor targeting is retained, by sulfhydryl-directed chromatography. Thus, precursor binding and translocation can be biochemically uncoupled, indicating that they are sequential reactions mediated by distinct components.
journal_name
Celljournal_title
Cellauthors
Nicchitta CV,Migliaccio G,Blobel Gdoi
10.1016/0092-8674(91)90091-csubject
Has Abstractpub_date
1991-05-17 00:00:00pages
587-98issue
4eissn
0092-8674issn
1097-4172pii
0092-8674(91)90091-Cjournal_volume
65pub_type
杂志文章相关文献
CELL文献大全abstract::Acquisition of the ability to produce and respond to a growth factor may result in increased cellular proliferation and could lead to malignant transformation. The fact that a large variety of tumor cells secrete transforming growth factor-alpha (TGF-alpha) suggests involvement of TGF-alpha in cellular transformation ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90747-6
更新日期:1986-07-18 00:00:00
abstract::The cocrystal structure of human poly(A)-binding protein (PABP) has been determined at 2.6 A resolution. PABP recognizes the 3' mRNA poly(A) tail and plays critical roles in eukaryotic translation initiation and mRNA stabilization/degradation. The minimal PABP used in this study consists of the N-terminal two RRM-type...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81517-2
更新日期:1999-09-17 00:00:00
abstract::We have cloned a Dictyostelium phosphotyrosine phosphatase (PTP1) with a catalytic domain showing approximately 38%-50% amino acid identity to those of other PTPs. PTP1 contains an approximately 99 amino acid insert and bacterially produced PTP1 possesses PTP activity. PTP1 is expressed at a very low level in vegetati...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90597-6
更新日期:1992-11-13 00:00:00
abstract::The yeast Sir2/3/4p complex is found in abundance at telomeres, where it participates in the formation of silent heterochromatin and telomere maintenance. Here, we show that Sir3p is released from telomeres in response to DNA double-strand breaks (DSBs), binds to DSBs, and mediates their repair, independent of cell ma...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80772-2
更新日期:1999-05-28 00:00:00
abstract::The endoplasmic reticulum (ER) often forms stacked membrane sheets, an arrangement that is likely required to accommodate a maximum of membrane-bound polysomes for secretory protein synthesis. How sheets are stacked is unknown. Here, we used improved staining and automated ultrathin sectioning electron microscopy meth...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.06.031
更新日期:2013-07-18 00:00:00
abstract::An examination of Abelson murine leukemia virus (A-MuL V)-hematopoietic cell interaction in cultures of fetal tissues reveals that A-MuLV can stimulate the formation of two different types of colonies. One type of colony is white and composed of A-MuLV-transformed lymphoid cells that can develop into established cell ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90035-0
更新日期:1981-10-01 00:00:00
abstract::Suppressor of Hairless [Su(H)]/Lag-1/RBP-Jkappa/CBF1 is the only known transducing transcription factor for Notch receptor signaling. Here, we show that Su(H) has three distinct functions in the development of external mechanosensory organs in Drosophila: Notch-dependent transcriptional activation and a novel auto-rep...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)00198-7
更新日期:2000-12-08 00:00:00
abstract::Appropriate cell number and organ size in a multicellular organism are determined by coordinated cell growth, proliferation, and apoptosis. Disruption of these processes can cause cancer. Recent studies have identified the Large tumor suppressor (Lats)/Warts (Wts) protein kinase as a key component of a pathway that co...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2004.12.036
更新日期:2005-03-11 00:00:00
abstract::Translation of ribosomal proteins in the alpha operon of E. coli is repressed by one of the encoded proteins, S4; it specifically recognizes an RNA fragment containing the translational initiation site for the first gene in the operon. RNA structure mapping experiments have suggested a pseudoknot structure for the S4 ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90123-2
更新日期:1989-05-19 00:00:00
abstract::Recent work has identified a mouse gene (tsg101) whose inactivation in fibroblasts results in cellular transformation and the ability to produce metastatic tumors in nude mice. Here, we report that the human homolog, TSG101, which we isolated and mapped to chromosome 11, bands 15.1-15.2, a region proposed to contain t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81866-8
更新日期:1997-01-10 00:00:00
abstract::Linear simian virus 40 (SV40) chromosomes were prepared by Eco R1 nuclease cleavage of the circular SV40 chromosomes released from virions with dithiothreitol at pH 9,8. Chromatin-DNA hybrids were constructed with segments of 3H-labeled, naked SV40 DNA covalently joined via the Eco R1-generated cohesive ends to segmen...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90279-9
更新日期:1978-11-01 00:00:00
abstract::Nuclear receptors undergo ligand-dependent conformational changes that are required for corepressor-coactivator exchange, but whether there is an actual requirement for specific epigenetic landmarks to impose ligand dependency for gene activation remains unknown. Here we report an unexpected and general strategy that ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2006.12.038
更新日期:2007-02-09 00:00:00
abstract::Rodent cells resistant to PALA, a specific inhibitor of the aspartate transcarbamylase activity of the multifunctional CAD protein, overproduce CAD as a result of amplification of the CAD gene. We cloned a functional CAD gene from Syrian hamster cells using a cosmid vector. Two independently isolated cosmids containin...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90410-4
更新日期:1981-12-01 00:00:00
abstract::Removal of histone H1 produces a transition in the structure of chromatin fibers as observed by electron microscopy. Chromatin containing all histone proteins appears as fibers with a diameter of about 250 A. The nucleosomes within these fibers are closely packed. If histone H1 is selectively removed with 50-100 mM Na...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(77)90188-x
更新日期:1977-09-01 00:00:00
abstract::Cyclic dinucleotides (CDNs) have been previously recognized as important secondary signaling molecules in bacteria and, more recently, in mammalian cells. In the former case, they represent secondary messengers affecting numerous responses of the prokaryotic cell, whereas in the latter, they act as agonists of the inn...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2013.08.014
更新日期:2013-08-29 00:00:00
abstract::Pulses of ecdysteroids direct Drosophila through its life cycle by activating stage- and tissue-specific genetic regulatory hierarchies. Here we show that an orphan nuclear receptor, DHR78, functions at the top of the ecdysteroid regulatory hierarchies. Null mutations in DHR78 lead to lethality during the third larval...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81184-8
更新日期:1998-05-15 00:00:00
abstract::Cultured preadipose 3T3 cells are induced or enabled to undergo adipose conversion in the presence of an extract of pituitary gland. Adipogenic activity is found in standard growth hormone preparations derived from different species. Further purification of rat growth hormone by several methods does not remove its adi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90440-8
更新日期:1982-07-01 00:00:00
abstract::Using positional cloning strategies, we have identified a CTG triplet repeat that undergoes expansion in myotonic dystrophy patients. This sequence is highly variable in the normal population. PCR analysis of the interval containing this repeat indicates that unaffected individuals have been 5 and 27 copies. Myotonic ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90154-5
更新日期:1992-02-21 00:00:00
abstract::The large tumor antigen (LTag) of simian virus 40, an AAA(+) protein, is a hexameric helicase essential for viral DNA replication in eukaryotic cells. LTag functions as an efficient molecular machine powered by ATP binding and hydrolysis for origin DNA melting and replication fork unwinding. To understand how ATP bind...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2004.09.017
更新日期:2004-10-01 00:00:00
abstract::Polarity in mammalian cells emerges from the assembly of signaling molecules into extensive biochemical interaction networks. Despite their complexity, bacterial pathogens have evolved parsimonious mechanisms to hijack these systems. Here, we develop a tractable experimental and theoretical model to uncover fundamenta...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.11.063
更新日期:2012-02-17 00:00:00
abstract::During aging, stromal functions are thought to be impaired, but little is known whether this stems from changes of fibroblasts. Using population- and single-cell transcriptomics, as well as long-term lineage tracing, we studied whether murine dermal fibroblasts are altered during physiological aging under different di...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.10.012
更新日期:2018-11-29 00:00:00
abstract::Seeing begins in the photoreceptors, where light is absorbed and signaled to the nervous system. Throughout the animal kingdom, photoreceptors are diverse in design and purpose. Nonetheless, phototransduction-the mechanism by which absorbed photons are converted into an electrical response-is highly conserved and base...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2009.09.029
更新日期:2009-10-16 00:00:00
abstract::This year marks the centenary of Robert Koch's Nobel Prize for discovering the cause of tuberculosis. Koch was also the first scientist to isolate the anthrax and cholera microbes. Yet perhaps one of his greatest contributions to biology is the least appreciated: his method for propagating individual colonies of bacte...
journal_title:Cell
pub_type: 传,历史文章,杂志文章
doi:10.1016/j.cell.2005.11.001
更新日期:2005-11-18 00:00:00
abstract::T cells expressing chimeric antigen receptors (CARs) are promising cancer therapeutic agents, with the prospect of becoming the ultimate smart cancer therapeutics. To expand the capability of CAR T cells, here, we present a split, universal, and programmable (SUPRA) CAR system that simultaneously encompasses multiple ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.03.038
更新日期:2018-05-31 00:00:00
abstract::Three recent papers reveal a fascinating link between pol II termination and ribonucleolytic decay of the nascent transcript by a 5'-3' exonuclease (yeast Rat1 and human Xrn2). The exonuclease travels with pol II and gains access to the nascent RNA after endonucleolytic cleavage at the poly(A) site or at a second cotr...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2004.11.041
更新日期:2004-12-29 00:00:00
abstract::Neural inputs from internal organs are essential for normal autonomic function. The vagus nerve is a key body-brain connection that monitors the digestive, cardiovascular, and respiratory systems. Within the gastrointestinal tract, vagal sensory neurons detect gut hormones and organ distension. Here, we investigate th...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.05.011
更新日期:2016-06-30 00:00:00
abstract::In this issue of Cell, two articles show that tumor-specific changes in HLA-mediated antigen presentation affect tumor immunogenicity and may play a role in shaping cancer cell survival. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2017.11.012
更新日期:2017-11-30 00:00:00
abstract::Here we characterize the T-cell-activating protein (TAP), an Ly-6 gene product involved in T cell activation, as a glycoprotein with a molecular weight of 10-12 kd under nonreducing conditions and 15-18 kd under reducing ones. Two of the three bands that are precipitated from metabolically labeled cells are expressed ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90593-3
更新日期:1986-11-07 00:00:00
abstract::During C. elegans embryogenesis an 8-cell stage blastomere, called MS, undergoes a reproducible cleavage pattern, producing pharyngeal cells, body wall muscles, and cell deaths. We show here that maternal-effect mutations in the pie-1 and mex-1 genes cause additional 8-cell stage blastomeres to adopt a fate very simil...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90542-k
更新日期:1992-07-10 00:00:00
abstract::Dysfunctional mitochondria accumulate in many human diseases. Accordingly, mitophagy, which removes these mitochondria through lysosomal degradation, is attracting broad attention. Due to uncertainties in the operational principles of conventional mitophagy probes, however, the specificity and quantitativeness of thei...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.04.025
更新日期:2020-05-28 00:00:00