Abstract：
:We have constructed a gene fusion using the promoter of Drosophila hsp70 and the structural gene for Drosophila alcohol dehydrogenase (Adh) and used this construct to transform Adh-deficient flies. In these transformants, Adh is expressed only after heat shock. Like hsp70 itself, this heat-shock-inducible Adh (Adhhs) is induced in a wide variety of tissues. It fails to be induced in primary spermatocytes. Although the tissue distribution of Adh activity is very different from wild type, this does not appear to be deleterious. Indeed, the induction of Adhhs allows flies to survive exposure to ethanol. We have used this latter characteristic to select dominant, trans-acting mutations that alter the response of flies to heat shock.
journal_name
Celljournal_title
Cellauthors
Bonner JJ,Parks C,Parker-Thornburg J,Mortin MA,Pelham HRdoi
10.1016/0092-8674(84)90432-xsubject
Has Abstractpub_date
1984-07-01 00:00:00pages
979-91issue
3eissn
0092-8674issn
1097-4172pii
0092-8674(84)90432-Xjournal_volume
37pub_type
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