Abstract:
:Adult neovascularization relies on the recruitment of circulating cells, but their angiogenic roles and recruitment mechanisms are unclear. We show that the endothelial growth factor VEGF is sufficient for organ homing of circulating mononuclear myeloid cells and is required for their perivascular positioning and retention. Recruited bone marrow-derived circulating cells (RBCCs) summoned by VEGF serve a function distinct from endothelial progenitor cells. Retention of RBCCs in close proximity to angiogenic vessels is mediated by SDF1, a chemokine induced by VEGF in activated perivascular myofibroblasts. RBCCs enhance in situ proliferation of endothelial cells via secreting proangiogenic activities distinct from locally induced activities. Precluding RBCCs strongly attenuated the proangiogenic response to VEGF and addition of purified RBCCs enhanced angiogenesis in excision wounds. Together, the data suggest a model for VEGF-programmed adult neovascularization highlighting the essential paracrine role of recruited myeloid cells and a role for SDF1 in their perivascular retention.
journal_name
Celljournal_title
Cellauthors
Grunewald M,Avraham I,Dor Y,Bachar-Lustig E,Itin A,Jung S,Chimenti S,Landsman L,Abramovitch R,Keshet Edoi
10.1016/j.cell.2005.10.036subject
Has Abstractpub_date
2006-01-13 00:00:00pages
175-89issue
1eissn
0092-8674issn
1097-4172pii
S0092-8674(05)01274-2journal_volume
124pub_type
杂志文章相关文献
CELL文献大全abstract::Recent studies have revealed that multiple intracellular signaling proteins may assemble into structured, yet sometimes infinite, higher-order signaling machines for transmission of receptor activation information to cellular responses. These studies advance our understanding of cell signaling and implicate new molecu...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.03.013
更新日期:2013-04-11 00:00:00
abstract::During metastasis, migrating breast cancer stem cells undergo a loss of polarity leading to an epithelial-to-mesenchymal transition (EMT). Gupta et al. (2009) use this attribute of cancer stem cells to develop a high-throughput screen, which successfully identifies small molecules that specifically inhibit cancer stem...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2009.08.007
更新日期:2009-08-21 00:00:00
abstract::The crystal structure of a proteolytic subfragment from scallop striated muscle myosin, complexed with MgADP, has been solved at 2.5 A resolution and reveals an unusual conformation of the myosin head. The converter and the lever arm are in very different positions from those in either the pre-power stroke or near-rig...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80756-4
更新日期:1999-05-14 00:00:00
abstract::V(D)J recombination of antigen receptor gene segments in B and T cells is mediated by the lymphoid-specific proteins RAG1 and RAG2. Now, Ji et al. (2010) demonstrate how RAG1 and RAG2 use DNA sequence specificity and modified histones within chromatin to target specific loci for V(D)J recombination at different stages...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2010.04.014
更新日期:2010-04-30 00:00:00
abstract::We have identified a yeast protein that binds specifically to the ARS consensus sequence. By two-step chromatography we have purified the factor to apparent homogeneity as a single polypeptide of 67 kd. The purified ARS consensus-binding protein (ACBP) recognizes the ARS consensus of the four genomic ARS elements test...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(91)90319-t
更新日期:1991-03-08 00:00:00
abstract::The axonal shafts of neurons contain bundled microtubules, whereas extending growth cones contain unbundled microtubule filaments, suggesting that localized activation of microtubule-associated proteins (MAP) at the transition zone may bundle these filaments during axonal growth. Dephosphorylation is thought to lead t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.03.023
更新日期:2007-05-04 00:00:00
abstract::Telomerase activity was demonstrated in cell-free extracts from S. cerevisiae through the use of a PCR-based assay. As expected, this activity was eliminated by RNase or phenol treatment of the extract and was dependent on dGTP and dTTP. Telomerase was not detected in extracts prepared from cells grown for approximate...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(05)80017-0
更新日期:1995-06-30 00:00:00
abstract::We have isolated the genes encoding the largest subunit of all three classes of RNA polymerase from Trypanosoma brucei. While the pol II largest subunit is encoded by a single gene in all organisms examined to date, trypanosomes contain two copies of the gene. Both genes are expressed in the procyclic and bloodstream ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90686-7
更新日期:1989-03-10 00:00:00
abstract::The classical model for the activation of the nucleotide exchange factor Son of sevenless (SOS) involves its recruitment to the membrane, where it engages Ras. The recent discovery that Ras*GTP is an allosteric activator of SOS indicated that the regulation of SOS is more complex than originally envisaged. We now pres...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2004.10.005
更新日期:2004-10-29 00:00:00
abstract::Maintaining the pluripotency of mouse ES cells requires both LIF (leukemia inhibitory factor) and unknown factors in serum. The paper from Ying et al. in this issue of Cell shows that BMP (bone morphogenetic protein) can replace serum in this capacity, defining molecular requirements for ES cell self-renewal. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/s0092-8674(03)00853-5
更新日期:2003-10-31 00:00:00
abstract::Dysfunction and loss of insulin-producing pancreatic beta cells represent hallmarks of diabetes mellitus. Here, we show that mice lacking the mitogen-activated protein kinase (MAPK) p38delta display improved glucose tolerance due to enhanced insulin secretion from pancreatic beta cells. Deletion of p38delta results in...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2008.11.018
更新日期:2009-01-23 00:00:00
abstract::ATP increases microtubule steady state assembly and disassembly rates in vitro in a concentration-dependent manner. Bovine brain microtubules, composed of 75% tubulin and 25% high molecular weight microtubule-associated proteins (MAPs), were purified by three cycles of assembly and disassembly in the absence of ATP. W...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90122-3
更新日期:1979-11-01 00:00:00
abstract::We have isolated a population of vesicular carriers involved in the transport (transcytosis) of proteins from the basolateral to the apical plasma membrane of hepatocytes. The obtained fraction was enriched in compartments containing known transcytosed proteins and depleted in elements of the secretory pathway, Golgi ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(91)90210-p
更新日期:1991-01-11 00:00:00
abstract::Type III secretion systems mediate export of virulence proteins and flagellar assembly subunits in Gram-negative bacteria. Chaperones specific to each class of secreted protein are believed to prevent degradation of the secreted substrates. We show that an additional role of chaperones may be to regulate translation o...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)00053-2
更新日期:2000-08-18 00:00:00
abstract::Apoptosis and autophagy are both tightly regulated biological processes that play a central role in tissue homeostasis, development, and disease. The anti-apoptotic protein, Bcl-2, interacts with the evolutionarily conserved autophagy protein, Beclin 1. However, little is known about the functional significance of thi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2005.07.002
更新日期:2005-09-23 00:00:00
abstract::We describe the identification and characterization of the Saccharomyces cerevisiae ZIP2 gene, which encodes a novel meiosis-specific protein essential for synaptonemal complex formation. In the zip2 mutant, chromosomes are homologously paired but not synapsed. The Zip2 protein localizes to discrete foci on meiotic ch...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81164-2
更新日期:1998-05-01 00:00:00
abstract::Rho and rac, two members of the ras-related superfamily of small GTPases, regulate the polymerization of actin to produce stress fibers and lamellipodia, respectively. We report here that cdc42, another member of the rho family, triggers the formation of a third type of actin-based structure found at the cell peripher...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(95)90370-4
更新日期:1995-04-07 00:00:00
abstract::During the SOS response, LexA repressor is inactivated by specific cleavage. Although cleavage requires RecA protein in vivo, RecA acts indirectly as a coprotease by stimulating an inherent self-cleavage activity of LexA. In lambda lysogens, cleavage of lambda Cl repressor in a similar but far slower reaction results ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90645-7
更新日期:1993-06-18 00:00:00
abstract::Ubiquitin-conjugating enzyme variant (UEV) proteins resemble ubiquitin-conjugating enzymes (E2s) but lack the defining E2 active-site residue. The MMS2-encoded UEV protein has been genetically implicated in error-free postreplicative DNA repair in Saccharomyces cerevisiae. We show that Mms2p forms a specific heteromer...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80575-9
更新日期:1999-03-05 00:00:00
abstract::Hematopoietic stem cells (HSCs) develop from a specialized subpopulation of endothelial cells known as hemogenic endothelium (HE). Although the HE origin of HSCs is now well established in different species, the signaling pathways that control this transition remain poorly understood. Here, we show that activation of ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.08.055
更新日期:2013-09-26 00:00:00
abstract::The yeast Sir2/3/4p complex is found in abundance at telomeres, where it participates in the formation of silent heterochromatin and telomere maintenance. Here, we show that Sir3p is released from telomeres in response to DNA double-strand breaks (DSBs), binds to DSBs, and mediates their repair, independent of cell ma...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80772-2
更新日期:1999-05-28 00:00:00
abstract::Mutations in the Drosophila maternal genes plutonium (plu) and pan gu (png) have the striking phenotype that DNA replication initiates in unfertilized eggs. Fertilized eggs from plu or png mutant mothers also have a mutant phenotype; DNA replication is uncoupled from nuclear division, resulting in giant, polyploid nuc...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(91)90050-9
更新日期:1991-09-20 00:00:00
abstract::The protein kinase Akt occupies a central position in multiple signaling pathways. Although numerous Akt substrates have been identified, less is known about the factors that regulate specific cellular responses to Akt signaling. In this issue, Schenck et al. (2008) demonstrate that the endosomal protein Appl1 modulat...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2008.04.015
更新日期:2008-05-02 00:00:00
abstract::Acquisition of the intestinal microbiota begins at birth, and a stable microbial community develops from a succession of key organisms. Disruption of the microbiota during maturation by low-dose antibiotic exposure can alter host metabolism and adiposity. We now show that low-dose penicillin (LDP), delivered from birt...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.05.052
更新日期:2014-08-14 00:00:00
abstract::Although a great deal is known biochemically about peroxiredoxins (Prxs), little is known about their real physiological function. We show here that two cytosolic yeast Prxs, cPrxI and II, which display diversity in structure and apparent molecular weights (MW), can act alternatively as peroxidases and molecular chape...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2004.05.002
更新日期:2004-05-28 00:00:00
abstract::All recombination models postulate one or more recombination intermediates that are joint molecules containing two homologous parental molecules. A spike of branched DNA molecules not seen in DNA from mitotic cells was found in the two-dimensional gel analysis of meiotic DNA from S. cerevisiae. The mass of molecules i...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90173-2
更新日期:1994-01-14 00:00:00
abstract::Receptor-mediated endocytosis of specific ligands is mediated through clustering of receptor-ligand complexes in coated pits on the cell surface. Following this clustering event, the ligand is internalized into a noncoated intracellular vesicle, the receptosome, which selectively avoids fusion with lysosomes and moves...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(80)90115-4
更新日期:1980-08-01 00:00:00
abstract::We have analyzed the transcriptional regulation of plastid genes during chloroplast development in illuminated spinach cotyledons and during leaf formation. The RNAs encoded by plastid genes accumulate with different kinetics during the developmental transitions. Using a novel plastid run-on transcription assay we dem...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90290-x
更新日期:1987-05-08 00:00:00
abstract::We predict regulatory targets for 14 Arabidopsis microRNAs (miRNAs) by identifying mRNAs with near complementarity. Complementary sites within predicted targets are conserved in rice. Of the 49 predicted targets, 34 are members of transcription factor gene families involved in developmental patterning or cell differen...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(02)00863-2
更新日期:2002-08-23 00:00:00
abstract::We have isolated five clones containing human histone genes from a human genomic DNA library, using a cloned X. laevis histone H4 cDNA probe (pX1ch4). Each genomic clone has been mapped and the locations of the histone genes have been determined by blot hybridization and hybridization-selection of human histone mRNA. ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90092-1
更新日期:1981-06-01 00:00:00