Identification and purification of a protein that binds the yeast ARS consensus sequence.

abstract：:The lack of in vitro prostate cancer models that recapitulate the diversity of human prostate cancer has hampered progress in understanding disease pathogenesis and therapy response. Using a 3D organoid system, we report success in long-term culture of prostate cancer from biopsy specimens and circulating tumor cells....

journal_title：Cell

authors： Gao D,Vela I,Sboner A,Iaquinta PJ,Karthaus WR,Gopalan A,Dowling C,Wanjala JN,Undvall EA,Arora VK,Wongvipat J,Kossai M,Ramazanoglu S,Barboza LP,Di W,Cao Z,Zhang QF,Sirota I,Ran L,MacDonald TY,Beltran H,Mosquera J

更新日期：2014-09-25 00:00:00

abstract：:DNA repair was measured in the dihydrofolate reductase gene in Chinese hamster ovary cells, amplified for the gene, by quantitating pyrimidine dimers with a specific UV-endonuclease. More than two thirds of the dimers had been removed from a 14.1 kb restriction fragment of the gene by 26 hr after irradiation (20 J/m2)...

journal_title：Cell

authors： Bohr VA,Smith CA,Okumoto DS,Hanawalt PC

更新日期：1985-02-01 00:00:00

abstract：:Constitutive egress of bone marrow (BM)-resident hematopoietic stem and progenitor cells (HSPCs) into the blood is a well-established phenomenon, but the ultimate fate and functional relevance of circulating HSPCs is largely unknown. We show that mouse thoracic duct (TD) lymph contains HSPCs that possess short- and lo...

journal_title：Cell

authors： Massberg S,Schaerli P,Knezevic-Maramica I,Köllnberger M,Tubo N,Moseman EA,Huff IV,Junt T,Wagers AJ,Mazo IB,von Andrian UH

更新日期：2007-11-30 00:00:00

abstract：:Vascular permeability is frequently associated with inflammation and is triggered by a cohort of secreted permeability factors such as vascular endothelial growth factor (VEGF). Here, we show that the physiological vascular permeability that precedes implantation is directly controlled by progesterone receptor (PR) an...

journal_title：Cell

authors： Goddard LM,Murphy TJ,Org T,Enciso JM,Hashimoto-Partyka MK,Warren CM,Domigan CK,McDonald AI,He H,Sanchez LA,Allen NC,Orsenigo F,Chao LC,Dejana E,Tontonoz P,Mikkola HK,Iruela-Arispe ML

更新日期：2014-01-30 00:00:00

abstract：:The triskelion shape of the clathrin molecule enables it to form the polyhedral protein network that covers clathrin-coated pits and vesicles. Domains within the clathrin heavy chain that are responsible for maintaining triskelion shape and function were identified and localized. Sequences that mediate trimerization a...

journal_title：Cell

authors： Näthke IS,Heuser J,Lupas A,Stock J,Turck CW,Brodsky FM

更新日期：1992-03-06 00:00:00

abstract：:Iron is ubiquitous in the environment and in biology. The study of iron biology focuses on physiology and homeostasis-understanding how cells and organisms regulate their iron content, how diverse tissues orchestrate iron allocation, and how dysregulated iron homeostasis leads to common hematological, metabolic, and n...

journal_title：Cell

authors： Hentze MW,Muckenthaler MU,Andrews NC

更新日期：2004-04-30 00:00:00

abstract：:The looming threat of a new influenza virus pandemic has fueled ambitious efforts to devise more predictive parameters for assessing the risks associated with emergent virus strains. At the same time, a comprehensive understanding of critical factors that can accurately predict the outcome of vaccination is sorely nee...

journal_title：Cell

authors： Miller MS,Palese P

更新日期：2014-04-10 00:00:00

abstract：:Thyroid hormone (T3) and retinoic acid (RA) receptors mediate ligand-dependent inhibition of epidermal growth factor (EGF) receptor and c-erbB2/neu promoter activities. Ligand-activated T3 and RA receptors act via a 36 bp 5' fragment of the EGF receptor gene in vivo and, in the presence of nuclear extract, bind with h...

journal_title：Cell

authors： Hudson LG,Santon JB,Glass CK,Gill GN

更新日期：1990-09-21 00:00:00

abstract：:Using cryo-electron microscopy, we have solved the structure of an icosidodecahedral COPII coat involved in cargo export from the endoplasmic reticulum (ER) coassembled from purified cargo adaptor Sec23-24 and Sec13-31 lattice-forming complexes. The coat structure shows a tetrameric assembly of the Sec23-24 adaptor la...

journal_title：Cell

authors： Stagg SM,LaPointe P,Razvi A,Gürkan C,Potter CS,Carragher B,Balch WE

更新日期：2008-08-08 00:00:00

abstract：:Planar polarity is a global, tissue-level phenomenon that coordinates cell behavior in a two-dimensional plane. The Frizzled/planar cell polarity (PCP) and anterior-posterior (AP) patterning systems for planar polarity operate in a variety of cell types and provide direction to cells with different morphologies and be...

journal_title：Cell

authors： Zallen JA

更新日期：2007-06-15 00:00:00

abstract：:In nematodes, a fraction of mRNAs contains a common 22 nucleotide 5' terminal spliced leader (SL) sequence derived by trans splicing. Here, we show that a cell-free extract prepared from developing embryos of the parasitic nematode Ascaris lumbricoides catalyzes accurate and efficient SL addition to a synthetic pre-mR...

journal_title：Cell

authors： Hannon GJ,Maroney PA,Denker JA,Nilsen TW

更新日期：1990-06-29 00:00:00

abstract：:The Escherichia coli relBE operon encodes a toxin-antitoxin pair, RelE-RelB. RelB can reverse inhibition of protein synthesis by RelE in vivo. We have found that although RelE does not degrade free RNA, it cleaves mRNA in the ribosomal A site with high codon specificity. Among stop codons UAG is cleaved with fast, UAA...

journal_title：Cell

authors： Pedersen K,Zavialov AV,Pavlov MY,Elf J,Gerdes K,Ehrenberg M

更新日期：2003-01-10 00:00:00

abstract：:What happens to organelles during mitosis, and how they are apportioned to each of the daughter cells, is not completely clear. We have devised a procedure to address whether Golgi membranes fuse with the Endoplasmic Reticulum (ER) during mitosis via the detection of interactions between ER and Golgi proteins. This pr...

journal_title：Cell

authors： Pecot MY,Malhotra V

更新日期：2004-01-09 00:00:00

abstract：:Overlapping cDNA clones from the translocation breakpoint region (TBR) gene, recently discovered at the neurofibromatosis type 1 locus and found to be interrupted by deletions and a t(17;22) translocation, have been sequenced. A 4 kb sequence of the transcript of the TBR gene has been compared with sequences of genomi...

journal_title：Cell

authors： Cawthon RM,Weiss R,Xu GF,Viskochil D,Culver M,Stevens J,Robertson M,Dunn D,Gesteland R,O'Connell P

更新日期：1990-07-13 00:00:00

abstract：:For the faithful initiation of transcription of exogenously added fibroin gene, a crude cellular extract was prepared from the posterior silk glands where fibroin gene is specifically transcribed. The faithful initiation was demonstrated by the assay with use of truncated fibroin gene templates and confirmed by nuclea...

journal_title：Cell

authors： Tsuda M,Suzuki Y

更新日期：1981-11-01 00:00:00

abstract：:We have examined the conformation of DNA cloned from the hypersensitive region 5' of the chicken adult beta-globin gene, in order to determine whether the unusual sensitivity to nucleases is related to altered secondary structure of the DNA. The region contains a large number of sites for methylation; we have studied ...

journal_title：Cell

authors： Nickol JM,Felsenfeld G

更新日期：1983-12-01 00:00:00

abstract：:The v-erbB gene of avian erythroblastosis virus (AEV) codes for an integral plasma membrane glycoprotein, gp74erbB. Expression of gp74erbB and its intracellular precursors, gp66erbB and gp68erbB, has been studied in cells transformed by two temperature-sensitive mutants of AEV. After shift to 42 degrees C, the process...

journal_title：Cell

authors： Beug H,Hayman MJ

更新日期：1984-04-01 00:00:00

abstract：:Type 2 diabetes (T2D) is a worldwide epidemic with a medical need for additional targeted therapies. Suppression of hepatic glucose production (HGP) effectively ameliorates diabetes and can be exploited for its treatment. We hypothesized that targeting PGC-1α acetylation in the liver, a chemical modification known to ...

journal_title：Cell

authors： Sharabi K,Lin H,Tavares CDJ,Dominy JE,Camporez JP,Perry RJ,Schilling R,Rines AK,Lee J,Hickey M,Bennion M,Palmer M,Nag PP,Bittker JA,Perez J,Jedrychowski MP,Ozcan U,Gygi SP,Kamenecka TM,Shulman GI,Schreiber SL,Gr

更新日期：2017-03-23 00:00:00

abstract：:PTEN is a frequently mutated tumor suppressor gene that opposes the PI3K/AKT pathway through dephosphorylation of phosphoinositide-3,4,5-triphosphate. Recently, nuclear compartmentalization of PTEN was found as a key component of its tumor-suppressive activity; however its nuclear function remains poorly defined. Here...

journal_title：Cell

authors： Song MS,Carracedo A,Salmena L,Song SJ,Egia A,Malumbres M,Pandolfi PP

更新日期：2011-01-21 00:00:00

abstract：:Balancing the capacity for protein maturation with changes in protein flux through the endoplasmic reticulum (ER) is crucial for maintaining ER homeostasis. In this issue, Merksamer et al. (2008) exploit a redox-sensitive fluorescent protein to monitor the environment inside the ER of living yeast, illuminating how th...

journal_title：Cell

authors： Kang SW,Hegde RS

更新日期：2008-11-28 00:00:00

abstract：:Evidence is now mounting that liquid-liquid phase separation (LLPS) underlies the formation of membraneless compartments in cells. This realization has motivated major efforts to delineate the function of such biomolecular condensates in normal cells and their roles in contexts ranging from development to age-related ...

journal_title：Cell

authors： Alberti S,Gladfelter A,Mittag T

更新日期：2019-01-24 00:00:00

abstract：:Proteolysis by the ubiquitin-proteasome pathway is often regulated, but the mechanisms underlying such regulation remain ill-defined. In Saccharomyces cerevisiae, cell type is controlled by the MAT transcription factors. The alpha2 repressor is a known ubiquitin pathway substrate in alpha haploid cells. We show that a...

journal_title：Cell

authors： Johnson PR,Swanson R,Rakhilina L,Hochstrasser M

更新日期：1998-07-24 00:00:00

abstract：:The Wee1 kinase phosphorylates and inhibits cyclin-dependent kinase 1 (Cdk1), thereby delaying entry into mitosis until appropriate conditions have been met. An understanding of the mechanisms that regulate Wee1 should provide new insight into how cells make the decision to enter mitosis. We report here that Swe1, the...

journal_title：Cell

authors： Harvey SL,Charlet A,Haas W,Gygi SP,Kellogg DR

更新日期：2005-08-12 00:00:00

abstract：:Enhancers provide critical information directing cell-type-specific transcriptional programs, regulated by binding of signal-dependent transcription factors and their associated cofactors. Here, we report that the most strongly activated estrogen (E2)-responsive enhancers are characterized by trans-recruitment and in ...

journal_title：Cell

authors： Liu Z,Merkurjev D,Yang F,Li W,Oh S,Friedman MJ,Song X,Zhang F,Ma Q,Ohgi KA,Krones A,Rosenfeld MG

更新日期：2014-10-09 00:00:00

abstract：:Fractionation of a microsomal detergent extract with ammonium sulfate allows separation of the signal recognition particle receptor (SR alpha), which is required for targeting of the nascent chain, from other microsomal proteins, such as signal peptidase, whose activity is displayed during subsequent translocation. Th...

journal_title：Cell

authors： Nicchitta CV,Migliaccio G,Blobel G

更新日期：1991-05-17 00:00:00

abstract：:Human hepatitis B virus core protein expressed in E. coli assembles into two sizes of particle. We have determined their three-dimensional structures by electron cryomicroscopy and image processing. The large and small particles correspond to triangulation number T = 4 and T = 3 dimer clustered packings, containing 24...

journal_title：Cell

authors： Crowther RA,Kiselev NA,Böttcher B,Berriman JA,Borisova GP,Ose V,Pumpens P

更新日期：1994-06-17 00:00:00

abstract：:The protein product of the human Duchenne muscular dystrophy locus (DMD) and its mouse homolog (mDMD) have been identified by using polyclonal antibodies directed against fusion proteins containing two distinct regions of the mDMD cDNA. The DMD protein is shown to be approximately 400 kd and to represent approximately...

journal_title：Cell

authors： Hoffman EP,Brown RH Jr,Kunkel LM

更新日期：1987-12-24 00:00:00

abstract：:Certain mutant alleles at the low density lipoprotein (LDL) receptor locus produce receptors that bind LDL normally, but fail to cluster in coated pits and therefore cannot transport LDL into cells. We prepared genomic DNA libraries from cells of two individuals with this phenotype (internalization-defective familial ...

journal_title：Cell

authors： Lehrman MA,Goldstein JL,Brown MS,Russell DW,Schneider WJ

更新日期：1985-07-01 00:00:00

abstract：:Brefeldin A (BFA) is a fungal metabolite that causes a redistribution of the stacked cisternae of the Golgi complex into the endoplasmic reticulum by inhibiting anterograde transport. We report that BFA also causes membrane tubules derived from the trans-Golgi network (TGN) to fuse with early endosomes. In the presenc...

journal_title：Cell

authors： Wood SA,Park JE,Brown WJ

更新日期：1991-11-01 00:00:00

abstract：:We have developed an in vitro system utilizing yeast cell-free extracts to catalyze recombination events between homologous plasmids containing different mutant alleles of the Tet or ARG4 genes. The reaction increased the frequency of Tcr or Arg+ transformants (recombinants) from 2 X 10(-6) to 1-3 X 10(-3). Linearizin...

journal_title：Cell

authors： Symington LS,Fogarty LM,Kolodner R

更新日期：1983-12-01 00:00:00