Abstract:
:Evidence is now mounting that liquid-liquid phase separation (LLPS) underlies the formation of membraneless compartments in cells. This realization has motivated major efforts to delineate the function of such biomolecular condensates in normal cells and their roles in contexts ranging from development to age-related disease. There is great interest in understanding the underlying biophysical principles and the specific properties of biological condensates with the goal of bringing insights into a wide range of biological processes and systems. The explosion of physiological and pathological contexts involving LLPS requires clear standards for their study. Here, we propose guidelines for rigorous experimental characterization of LLPS processes in vitro and in cells, discuss the caveats of common experimental approaches, and point out experimental and theoretical gaps in the field.
journal_name
Celljournal_title
Cellauthors
Alberti S,Gladfelter A,Mittag Tdoi
10.1016/j.cell.2018.12.035subject
Has Abstractpub_date
2019-01-24 00:00:00pages
419-434issue
3eissn
0092-8674issn
1097-4172pii
S0092-8674(18)31649-0journal_volume
176pub_type
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