当前位置： SCI文献检索 > CELL期刊下所有文献 > The synthesis and distribution of desmin and vimentin during myogenesis in vitro.

The synthesis and distribution of desmin and vimentin during myogenesis in vitro.

Abstract：

:Electrophoretic and autoradiographic analyses of the incorporation of 35S--methionine into newly synthesized proteins during myogenesis reveal that presumptive chicken myoblasts synthesize primarily one intermediate filament protein: vimentin. Desmin synthesis is initiated at the onset of fusion. Synthesis rates of both filament subunits increase during the first three days in culture, relative to the total protein synthesis rate. The observed increase in the rate of desmin synthesis (at least 10 fold) is significantly greater than that observed for vimentin, and is responsible for a net increase in the cellular desmin content relative to vimentin. Both filament subunits continue to be synthesized through at least 20 days in culture. Immunofluorescent staining using desmin- and vimentin-specific antisera supports the conclusion that desmin is synthesized only in fusing or multinucleate cells. These results indicate that the synthesis of the two filament subunits is not coordinately regulated during myogenesis. The distributions of desmin and vimentin in multinucleate chicken myotubes are indistinguishable, as determined by double immunofluorescence techniques. In early myotubes, both proteins are found in an intricate network of free cytoplasmic filaments. Later in myogenesis, several days after the appearance of alpha--actinin-containing Z line striations, both filament proteins become associated with the Z lines of newly assembled myofibrils, with a corresponding decrease in the number of cytoplasmic filaments. This transition corresponds to the time when the alpha--actinin-containing Z lines become aligned laterally. These data suggest that the two intermediate filament systems, desmin and vimentin, have an important role in the lateral organization and registration of myofibrils and that the synthesis of desmin and assembly of desmin-containing intermediate filaments during myogenesis is directly related to these functions. These results also indicate that the Z disc is assembled in at least two distinct steps during myogenesis.

journal_name

Cell

journal_title

Cell

authors

Gard DL,Lazarides E

doi

10.1016/0092-8674(80)90408-0

subject

Has Abstract

pub_date

1980-01-01 00:00:00

pages

263-75

issue

1

eissn

0092-8674

issn

1097-4172

pii

0092-8674(80)90408-0

journal_volume

19

pub_type

杂志文章

相关文献

CELL文献大全
  • Avant garde fatty acid synthesis by trypanosomes.

    abstract：:In this issue of Cell, Lee et al. (2006) report that the parasite Trypanosoma brucei synthesizes fatty acids in an unconventional way. T. brucei and two other trypanosomes use enzymes called elongases to synthesize myristate, a fourteen carbon (C14) fatty acid essential for pathogenesis. This is an unexpected finding ...

    journal_title：Cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.cell.2006.08.001

    authors： Cronan JE

    更新日期：2006-08-25 00:00:00

  • In trypanosomes the homolog of the largest subunit of RNA polymerase II is encoded by two genes and has a highly unusual C-terminal domain structure.

    abstract：:We have isolated the genes encoding the largest subunit of all three classes of RNA polymerase from Trypanosoma brucei. While the pol II largest subunit is encoded by a single gene in all organisms examined to date, trypanosomes contain two copies of the gene. Both genes are expressed in the procyclic and bloodstream ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(89)90686-7

    authors： Smith JL,Levin JR,Ingles CJ,Agabian N

    更新日期：1989-03-10 00:00:00

  • Regulation of DLG localization at synapses by CaMKII-dependent phosphorylation.

    abstract：:Discs large (DLG) mediates the clustering of synaptic molecules. Here we demonstrate that synaptic localization of DLG itself is regulated by CaMKII. We show that DLG and CaMKII colocalize at synapses and exist in the same protein complex. Constitutively activated CaMKII phenocopied structural abnormalities of dlg mut...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/s0092-8674(00)81964-9

    authors： Koh YH,Popova E,Thomas U,Griffith LC,Budnik V

    更新日期：1999-08-06 00:00:00

  • Nerve growth factor is an autocrine survival factor for memory B lymphocytes.

    abstract：:Production of nerve growth factor (NGF) was assessed in cultures of human T and B lymphocytes and macrophages. NGF was constitutively produced by B cells only, which also expressed surface p140trk-A and p75NGFR molecules and hence efficiently bound and internalized the cytokine. Neutralization of endogenous NGF caused...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/s0092-8674(00)81113-7

    authors： Torcia M,Bracci-Laudiero L,Lucibello M,Nencioni L,Labardi D,Rubartelli A,Cozzolino F,Aloe L,Garaci E

    更新日期：1996-05-03 00:00:00

  • Developmental activation of the H-2K gene is correlated with an increase in DNA methylation.

    abstract：:Embryonal carcinoma cell lines present strong developmental analogies with early embryonic cells. Previous studies have shown that treatment of F9 teratocarcinoma cells with retinoic acid induces the expression of the classical transplantation antigens which are indispensable for effective interactions between cells. ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(83)90179-4

    authors： Tanaka K,Appella E,Jay G

    更新日期：1983-12-01 00:00:00

  • Peroxisomes Get Loud: A Redox Antidote to Hearing Loss.

    abstract：:Pejvakin (PJVK), a protein originally identified in Persian families with sensorineural hearing loss, regulates peroxisomal dynamics and the antioxidant defense triggered by noise exposure in hair cells and auditory neurons of the inner ear. These findings bring peroxisomes to the forefront of noise-induced hearing lo...

    journal_title：Cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.cell.2015.10.060

    authors： Mardones P,Hetz C

    更新日期：2015-11-05 00:00:00

  • Quantitative and dynamic assessment of the contribution of the ER to phagosome formation.

    abstract：:Phagosomes were traditionally thought to originate from an invagination and scission of the plasma membrane to form a distinct intracellular vacuole. An alternative model implicating the endoplasmic reticulum (ER) as a major component of nascent and maturing phagosomes was recently proposed (Gagnon et al., 2002). To r...

    journal_title：Cell

    pub_type： 信件

    doi：10.1016/j.cell.2005.08.018

    authors： Touret N,Paroutis P,Terebiznik M,Harrison RE,Trombetta S,Pypaert M,Chow A,Jiang A,Shaw J,Yip C,Moore HP,van der Wel N,Houben D,Peters PJ,de Chastellier C,Mellman I,Grinstein S

    更新日期：2005-10-07 00:00:00

  • Increased expression of neurofilament subunit NF-L produces morphological alterations that resemble the pathology of human motor neuron disease.

    abstract：:Excessive accumulation of neurofilaments in the cell bodies and proximal axons of motor neurons is a major pathological hallmark during the early stages of many human motor neuron diseases. To test directly the consequence of overexpression of the major neurofilament subunit NF-L, we produced transgenic mice that accu...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(93)90157-l

    authors： Xu Z,Cork LC,Griffin JW,Cleveland DW

    更新日期：1993-04-09 00:00:00

  • Structural Insights into the Process of GPCR-G Protein Complex Formation.

    abstract：:The crystal structure of the β2-adrenergic receptor (β2AR) bound to the G protein adenylyl cyclase stimulatory G protein (Gs) captured the complex in a nucleotide-free state (β2AR-Gsempty). Unfortunately, the β2AR-Gsempty complex does not provide a clear explanation for G protein coupling specificity. Evidence from se...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2019.04.021

    authors： Liu X,Xu X,Hilger D,Aschauer P,Tiemann JKS,Du Y,Liu H,Hirata K,Sun X,Guixà-González R,Mathiesen JM,Hildebrand PW,Kobilka BK

    更新日期：2019-05-16 00:00:00

  • Biochemical fractionation and assembly of the membrane components that mediate nascent chain targeting and translocation.

    abstract：:Fractionation of a microsomal detergent extract with ammonium sulfate allows separation of the signal recognition particle receptor (SR alpha), which is required for targeting of the nascent chain, from other microsomal proteins, such as signal peptidase, whose activity is displayed during subsequent translocation. Th...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(91)90091-c

    authors： Nicchitta CV,Migliaccio G,Blobel G

    更新日期：1991-05-17 00:00:00

  • Identification of mouse sperm SED1, a bimotif EGF repeat and discoidin-domain protein involved in sperm-egg binding.

    abstract：:We report the identification of SED1, a protein required for mouse sperm binding to the egg zona pellucida. SED1 is homologous to a small group of secreted cell-matrix adhesive proteins that contain Notch-like EGF repeats and discoidin/F5/8 type C domains. SED1 is expressed in spermatogenic cells and is secreted by th...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/s0092-8674(03)00643-3

    authors： Ensslin MA,Shur BD

    更新日期：2003-08-22 00:00:00

  • In and out of the replication factory.

    abstract：:In this issue of Cell, use live-fluorescence microscopy to monitor individual genomic loci as they replicate in budding yeast. They confirm that DNA is recruited to replication factories and show that sister replication forks initiated from the same origin are held together within a single replication factory. ...

    journal_title：Cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.cell.2006.06.014

    authors： Meister P,Taddei A,Gasser SM

    更新日期：2006-06-30 00:00:00

  • Gene transfer of H-2 class II genes: antigen presentation by mouse fibroblast and hamster B-cell lines.

    abstract：:We have transferred the mouse Ak alpha and Ak beta genes, which encode the class II I-Ak molecule, into mouse L-cell fibroblasts and hamster B cells. I-Ak molecules are expressed on the surface of both cell types. The L-cell and hamster B-cell I-Ak molecules appear normal by serological analyses and two-dimensional ge...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(84)90225-3

    authors： Malissen B,Price MP,Goverman JM,McMillan M,White J,Kappler J,Marrack P,Pierres A,Pierres M,Hood L

    更新日期：1984-02-01 00:00:00

  • Contact with epithelial cells induces the formation of surface appendages on Salmonella typhimurium.

    abstract：:The enteric bacteria Salmonella typhimurium has the ability to invade (enter) nonphagocytic cells. The internalization process occurs as a result of an intimate interaction between the bacteria and the host cell, in which S. typhimurium triggers a cascade of host cell-signaling events leading to the formation of host ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(94)90510-x

    authors： Ginocchio CC,Olmsted SB,Wells CL,Galán JE

    更新日期：1994-02-25 00:00:00

  • SnapShot: receptor dynamics at plastic synapses.

    abstract：:The dynamic synapse is represented by the constant mobility and exchange of components at both the cell surface and at intracellular sites. This includes thermally powered Brownian diffusion movement, followed by reversible trapping through receptor-scaffold interactions and active transport of cargo vesicles through ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2014.06.002

    authors： Kneussel M,Triller A,Choquet D

    更新日期：2014-06-19 00:00:00

  • A molecular link tethering neuronal responses with the past.

    abstract：:Neurons need to alter their response to a given stimulus over time in order for the animal to modify its behavior within a changing environment. Chen et al. now demonstrate that neuronal structure and function are altered coordinately by the history of the cell's activity through an unexpected molecular pathway. ...

    journal_title：Cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.cell.2012.09.008

    authors： Della Santina L,Wong RO

    更新日期：2012-09-28 00:00:00

  • mTORC1 Activator SLC38A9 Is Required to Efflux Essential Amino Acids from Lysosomes and Use Protein as a Nutrient.

    abstract：:The mTORC1 kinase is a master growth regulator that senses many environmental cues, including amino acids. Activation of mTORC1 by arginine requires SLC38A9, a poorly understood lysosomal membrane protein with homology to amino acid transporters. Here, we validate that SLC38A9 is an arginine sensor for the mTORC1 path...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2017.09.046

    authors： Wyant GA,Abu-Remaileh M,Wolfson RL,Chen WW,Freinkman E,Danai LV,Vander Heiden MG,Sabatini DM

    更新日期：2017-10-19 00:00:00

  • Intron sequences involved in lariat formation during pre-mRNA splicing.

    abstract：:We have shown that lariat formation during in vitro splicing of several RNA precursors, from Drosophila to man, occurs at a unique and identifiable but weakly conserved site, 18 to 37 nucleotides proximal to the 3' splice site. Lariat formation within an artificial intron lacking a normal branch-point sequence occurs ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(85)90064-9

    authors： Reed R,Maniatis T

    更新日期：1985-05-01 00:00:00

  • The product of the mouse Xist gene is a 15 kb inactive X-specific transcript containing no conserved ORF and located in the nucleus.

    abstract：:The Xist gene maps to the X inactivation center region in both mouse and human, and previous analysis of the 3' end of the gene has demonstrated inactive X-specific expression, suggesting a possible role in X inactivation. We have now analyzed the entire mouse Xist gene. The mature inactive X-specific transcript is 15...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(92)90519-i

    authors： Brockdorff N,Ashworth A,Kay GF,McCabe VM,Norris DP,Cooper PJ,Swift S,Rastan S

    更新日期：1992-10-30 00:00:00

  • Communication by touch: role of cellular extensions in complex animals.

    abstract：:Neurons make networks of intercellular connections. Many other cells also send out long cellular extensions and "touch" other cells far away. The extensions are intriguing, but what do they do? Possible rationales for this cell behavior include: (1) forming precise binary connections with the possibility of forming mo...

    journal_title：Cell

    pub_type： 杂志文章,评审

    doi：10.1016/s0092-8674(03)00156-9

    authors： Rørth P

    更新日期：2003-03-07 00:00:00

  • Swaying is a mutant allele of the proto-oncogene Wnt-1.

    abstract：:Mice homozygous for the recessive mutation swaying (sw) are characterized by ataxia and hypertonia, attributed to the malformation of anterior regions of the cerebellum. We show that sw is a deletion of a single base pair from the proto-oncogene Wnt-1. The deletion is predicted to cause premature termination of transl...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(91)90369-a

    authors： Thomas KR,Musci TS,Neumann PE,Capecchi MR

    更新日期：1991-11-29 00:00:00

  • Regulation of armadillo by a Drosophila APC inhibits neuronal apoptosis during retinal development.

    abstract：:We find that inactivation of a Drosophila homolog of the tumor suppressor APC (D-APC) causes retinal neuronal degeneration and pigment cell hypertrophy, a phenotype remarkably similar to that found in humans with germline APC mutations. Retinal degeneration in the D-APC mutant results from apoptotic cell death, which ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/s0092-8674(00)81461-0

    authors： Ahmed Y,Hayashi S,Levine A,Wieschaus E

    更新日期：1998-06-26 00:00:00

  • Function of Neurospora mitochondrial tyrosyl-tRNA synthetase in RNA splicing requires an idiosyncratic domain not found in other synthetases.

    abstract：:Neurospora mitochondrial tyrosyl-tRNA synthetase (mt TyrRS), which is encoded by nuclear gene cyt-18, functions in splicing group I introns. Analysis of intragenic partial revertants of the cyt-18-2 mutant and in vitro mutants of the cyt-18 protein expressed in E. coli showed that splicing activity of the cyt-18 prote...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(90)90119-y

    authors： Cherniack AD,Garriga G,Kittle JD Jr,Akins RA,Lambowitz AM

    更新日期：1990-08-24 00:00:00

  • Mistranslation in a eucaryotic organism.

    abstract：:Previous work from our laboratory has demonstrated that a subclass of the aminoglycoside antibiotics, those containing the drug fragment paromamine, stimulates mistranslation in cell-free protein-synthesizing systems derived from eucaryotic cells. We report here experiments which show that the ciliate Tetrahymena ther...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(78)90201-5

    authors： Palmer E,Wilhelm JM

    更新日期：1978-02-01 00:00:00

  • TFIIH plays an essential role in RNA polymerase I transcription.

    abstract：:TFIIH is a multisubunit protein complex that plays an essential role in nucleotide excision repair and transcription of protein-coding genes. Here, we report that TFIIH is also required for ribosomal RNA synthesis in vivo and in vitro. In yeast, pre-rRNA synthesis is impaired in TFIIH ts strains. In a mouse, part of c...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/s0092-8674(02)00729-8

    authors： Iben S,Tschochner H,Bier M,Hoogstraten D,Hozák P,Egly JM,Grummt I

    更新日期：2002-05-03 00:00:00

  • Specialized piRNA pathways act in germline and somatic tissues of the Drosophila ovary.

    abstract：:In Drosophila gonads, Piwi proteins and associated piRNAs collaborate with additional factors to form a small RNA-based immune system that silences mobile elements. Here, we analyzed nine Drosophila piRNA pathway mutants for their impacts on both small RNA populations and the subcellular localization patterns of Piwi ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2009.03.040

    authors： Malone CD,Brennecke J,Dus M,Stark A,McCombie WR,Sachidanandam R,Hannon GJ

    更新日期：2009-05-01 00:00:00

  • Transposons in place of telomeric repeats at a Drosophila telomere.

    abstract：:We present the first isolation of the terminal DNA of an intact Drosophila telomere. It differs from those isolated from other eukaryotes by the lack of short tandem repeats at the terminus. The terminal 14.5 kb is composed of four tandem elements derived from two families of non-long terminal repeat retrotransposons ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(93)90318-k

    authors： Levis RW,Ganesan R,Houtchens K,Tolar LA,Sheen FM

    更新日期：1993-12-17 00:00:00

  • The structure of the human histone genes: clustered but not tandemly repeated.

    abstract：:We have isolated five clones containing human histone genes from a human genomic DNA library, using a cloned X. laevis histone H4 cDNA probe (pX1ch4). Each genomic clone has been mapped and the locations of the histone genes have been determined by blot hybridization and hybridization-selection of human histone mRNA. ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(81)90092-1

    authors： Heintz N,Zernik M,Roeder RG

    更新日期：1981-06-01 00:00:00

  • Synthesis of hybrid bacterial plasmids containing highly repeated satellite DNA.

    abstract：:Hybrid plasmid molecules containing tandemly repeated Drosophila satellite DNA were constructed using a modification of the (dA)-(dT) homopolymer procedure of Lobban and Kaiser (1973). Recombinant plasmids recovered after transformation of recA bacteria contained 10% of the amount of satellite DNA present in the trans...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(77)90038-1

    authors： Brutlag D,Fry K,Nelson T,Hung P

    更新日期：1977-03-01 00:00:00

  • DNAase I encapsulated in liposomes can induce neoplastic transformation of Syrian hamster embryo cells in culture.

    abstract：:We have used liposomes to deliver DNAase I inside normal Syrian hamster embryo (SHE) cells. We showed the entrance of DNAase I inside the cell by dose-dependent cytotoxicity; and the entrance of DNAase I into the nucleus by the induction of chromosomal aberrations and somatic mutation at the HPRT locus (but not at the...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(84)90450-1

    authors： Zajac-Kaye M,Ts'o PO

    更新日期：1984-12-01 00:00:00