Abstract：
:Previous work from our laboratory has demonstrated that a subclass of the aminoglycoside antibiotics, those containing the drug fragment paromamine, stimulates mistranslation in cell-free protein-synthesizing systems derived from eucaryotic cells. We report here experiments which show that the ciliate Tetrahymena thermophila (formerly T. pyriformis, syngen 1) is sensitive to the paromamine-containing aminoglycoside antibiotics. The drugs are active with respect to growth inhibition, inhibition of protein synthesis in the whole organism, inhibition of protein synthesis in vitro and the stimulation of mistranslation in cell-free protein-synthesizing systems. Because of their misreading properties, these drugs may be useful in isolating and propagating strains carrying mutations which can be translationally suppressed (that is, missense and nonsense mutations).
journal_name
Celljournal_title
Cellauthors
Palmer E,Wilhelm JMdoi
10.1016/0092-8674(78)90201-5subject
Has Abstractpub_date
1978-02-01 00:00:00pages
329-34issue
2eissn
0092-8674issn
1097-4172pii
0092-8674(78)90201-5journal_volume
13pub_type
杂志文章相关文献
CELL文献大全abstract：:A new type of mutation that affects a discrete step in the process of adsorptive endocytosis has been identified in one of 22 strains of fibroblasts derived from subjects with the clinical phenotype of homozygous familial hypercholesterolemia (FH). In this unique mutant strain (derived from subject J.D.), the cell sur...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(76)90130-6
更新日期：1976-12-01 00:00:00
abstract：:The Mre11/Rad50 complex has been implicated in the early steps of DNA double-strand break (DSB) repair through homologous recombination in several organisms. However, the enzymatic properties of this complex are incompatible with the generation of 3' single-stranded DNA for recombinase loading and strand exchange. In ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2008.09.054
更新日期：2008-10-17 00:00:00
abstract：:The crystal structure of the Src SH2 domain complexed with a high affinity 11-residue phosphopeptide has been determined at 2.7 A resolution by X-ray diffraction. The peptide binds in an extended conformation and makes primary interactions with the SH2 domain at six central residues: PQ(pY)EEI. The phosphotyrosine and...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(93)90405-f
更新日期：1993-03-12 00:00:00
abstract：:Malignant gliomas are aggressive brain tumors with limited therapeutic options, and improvements in treatment require a deeper molecular understanding of this disease. As in other cancers, recent studies have identified highly tumorigenic subpopulations within malignant gliomas, known generally as cancer stem cells. H...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2011.06.006
更新日期：2011-07-08 00:00:00
abstract：:M-MuLV-specific DNA probes were used to establish the state of integration and amplification of recombinant proviral sequences in Moloney virus-induced tumors of Balb/Mo, Balb/c and 129 mice. The somatically acquired viral sequences contain both authentic M-MuLV genomes and recombinants of M-MuLV with endogenous viral...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90099-4
更新日期：1981-06-01 00:00:00
abstract：:Upon heat shock, Drosophila Kc cells still contain normal cellular messenger RNAs in the cytoplasm. The distribution of these 25 degrees C mRNAs between polysomes and the postpolysomal fraction of heat-shocked cells appears unaltered as compared with control cells. The translatability of these normal cellular messages...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90155-0
更新日期：1981-02-01 00:00:00
abstract：:Cytotoxic T lymphocytes and lymphocytes with NK-like activity contain a serine esterase activity which has been localized to their cytoplasmic granules by cytochemistry and subcellular fractionation studies. The serine esterase-specific inhibitor 3H-DFP labels two protein species in the granules. The two proteins, ref...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(86)90441-1
更新日期：1986-10-24 00:00:00
abstract：:Embryonal carcinoma cell lines present strong developmental analogies with early embryonic cells. Previous studies have shown that treatment of F9 teratocarcinoma cells with retinoic acid induces the expression of the classical transplantation antigens which are indispensable for effective interactions between cells. ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90179-4
更新日期：1983-12-01 00:00:00
abstract：:Interactions between transcription factors bound to separate operator sites commonly play an important role in gene regulation by mediating cooperative binding to the DNA. However, few detailed structural models for understanding the molecular basis of such cooperativity are available. The c1 repressor of bacteriophag...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80891-0
更新日期：2000-06-23 00:00:00
abstract：:During yeast cell division, aggregates of damaged proteins are segregated asymmetrically between the bud and the mother. It is thought that protein aggregates are cleared from the bud via actin cable-based retrograde transport toward the mother and that Bni1p formin regulates this transport. Here, we examined the dyna...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2011.11.002
更新日期：2011-11-23 00:00:00
abstract：:Bacteria are known to communicate primarily via secreted extracellular factors. Here we identify a previously uncharacterized type of bacterial communication mediated by nanotubes that bridge neighboring cells. Using Bacillus subtilis as a model organism, we visualized transfer of cytoplasmic fluorescent molecules bet...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2011.01.015
更新日期：2011-02-18 00:00:00
abstract：:Fragile X syndrome results from the expansion of a CGG repeat that is normally interrupted by occasional AGG triplets. Linkage disequilibrium suggests that certain normal haplotypes may contribute unequally to the pool of fragile X chromosomes. Sequence analysis of normal alleles demonstrates haplotype-specific variat...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(94)90134-1
更新日期：1994-06-17 00:00:00
abstract：:Calmodulin is implicated as the primary transducer of the calcium signal in pancreatic beta cells, where it is present at very high concentrations. We have produced three lines of transgenic mice carrying a calmodulin minigene regulated by the rat insulin II promoter. Immunohistochemistry and hybridization analyses in...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(89)90505-9
更新日期：1989-09-22 00:00:00
abstract：:It is established that tumor cell-derived VEGF acts on endothelial cells to promote angiogenesis and tumor growth. Here, we demonstrate that in K5-SOS-dependent mouse skin tumors, autocrine VEGF is required for tumor cell proliferation in a cell-autonomous and angiogenesis-independent manner. VEGF is upregulated in SO...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2009.12.046
更新日期：2010-01-22 00:00:00
abstract：:Subcellular compartments in which folding and assembly of proteins occur seem to have a set of PCB proteins capable of mediating these and related processes, such as translocation across membranes. When a domain of a polypeptide chain emerges from a ribosome during synthesis or from the distal side of a membrane durin...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/0092-8674(89)90005-6
更新日期：1989-11-17 00:00:00
abstract：:Two-component signal transduction systems are the predominant means by which bacteria sense and respond to environmental stimuli. Bacteria often employ tens or hundreds of these paralogous signaling systems, comprised of histidine kinases (HKs) and their cognate response regulators (RRs). Faithful transmission of info...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2008.04.040
更新日期：2008-06-13 00:00:00
abstract：:The yeast Sir2/3/4p complex is found in abundance at telomeres, where it participates in the formation of silent heterochromatin and telomere maintenance. Here, we show that Sir3p is released from telomeres in response to DNA double-strand breaks (DSBs), binds to DSBs, and mediates their repair, independent of cell ma...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80772-2
更新日期：1999-05-28 00:00:00
abstract：:Dimerization-driven activation of the intracellular kinase domains of the epidermal growth factor receptor (EGFR) upon extracellular ligand binding is crucial to cellular pathways regulating proliferation, migration, and differentiation. Inactive EGFR can exist as both monomers and dimers, suggesting that the mechanis...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2012.12.030
更新日期：2013-01-31 00:00:00
abstract：:In this issue, Baumann et al. (2007) identify a helicase PICH that localizes to "threads" that remain connected between sister kinetochores after they have separated in anaphase. These threads are thought to be catenated centromeric DNA. PICH contributes to the mitotic checkpoint by recruiting Mad2 to kinetochores and...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2006.12.022
更新日期：2007-01-12 00:00:00
abstract：:The advent of H7N9 in early 2013 is of concern for a number of reasons, including its capability to infect humans, the lack of clarity in the etiology of infection, and because the human population does not have pre-existing immunity to the H7 subtype. Earlier sequence analyses of H7N9 hemagglutinin (HA) point to amin...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.05.034
更新日期：2013-06-20 00:00:00
abstract：:The yeast SIR2 gene is involved in regulating nucleosome phasing and transcription in the mating type system. We have found that SIR2 also plays another important role in the cell. Specifically, in wild-type SIR2 strains recombination between the tandemly repeated ribosomal RNA genes is depressed. In sir2 mutants, bot...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(89)90681-8
更新日期：1989-03-10 00:00:00
abstract：:Spinocerebellar ataxia type 1 (SCA1) is one of nine inherited, typically adult onset, polyglutamine neurodegenerative diseases. To examine whether development impacts SCA1, we used a conditional transgenic mouse model of SCA1 to delay the postnatal expression of mutant ATXN1 until after completion of cerebellar develo...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2006.09.036
更新日期：2006-11-17 00:00:00
abstract：:Netrins are bifunctional: they attract some axons and repel others. Netrin receptors of the Deleted in Colorectal Cancer (DCC) family are implicated in attraction and those of the UNC5 family in repulsion, but genetic evidence also suggests involvement of the DCC protein UNC-40 in some cases of repulsion. To test whet...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80804-1
更新日期：1999-06-25 00:00:00
abstract：:The HIV auxiliary protein Vpr potently blocks the cell cycle at the G2/M transition. Here, we show that G2/M arrest results from untimely activation of the structure-specific endonuclease (SSE) regulator SLX4 complex (SLX4com) by Vpr, a process that requires VPRBP-DDB1-CUL4 E3-ligase complex. Direct interaction of Vpr...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.12.011
更新日期：2014-01-16 00:00:00
abstract：:Centrosomes are non-membrane-bound compartments that nucleate microtubule arrays. They consist of nanometer-scale centrioles surrounded by a micron-scale, dynamic assembly of protein called the pericentriolar material (PCM). To study how PCM forms a spherical compartment that nucleates microtubules, we reconstituted P...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.05.028
更新日期：2017-06-01 00:00:00
abstract：:The Eph receptor tyrosine kinase family is regulated by autophosphorylation within the juxtamembrane region and the kinase activation segment. We have solved the X-ray crystal structure to 1.9 A resolution of an autoinhibited, unphosphorylated form of EphB2 comprised of the juxtamembrane region and the kinase domain. ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(01)00496-2
更新日期：2001-09-21 00:00:00
abstract：:A diverse range of ultimate chemical carcinogens inhibited the transfer of methyl groups from S-adenosylmethionine to hemimethylated DNA in a reaction catalyzed by mouse spleen methyltransferase. The formation of alkali-labile sites in DNA lessened its ability to accept methyl groups in vitro, but the methylation reac...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90514-7
更新日期：1983-01-01 00:00:00
abstract：:Pathogenic and other cytoplasmic DNAs activate the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway to induce inflammation via transcriptional activation by IRF3 and nuclear factor κB (NF-κB), but the functional consequences of exposing cGAS to chromosomes upon mitotic nuclear envelope bre...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2019.05.035
更新日期：2019-07-11 00:00:00
abstract：:Deletion analysis was carried out on the human 7SK RNA gene to map regions essential for in vitro transcription by RNA polymerase III. The sequence promoting transcription is located between 37 and 3 bp upstream of the 7SK RNA coding region. RNA polymerase III transcription of adjacent plasmid sequences can be directe...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(87)90012-2
更新日期：1987-10-09 00:00:00
abstract：:Histone acetyltransferases (HATs) and histone deacetylases (HDACs) conduct many critical functions through nonhistone substrates in metazoans, but only chromatin-associated nonhistone substrates are known in Saccharomyces cerevisiae. Using yeast proteome microarrays, we identified and validated many nonchromatin subst...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2009.01.033
更新日期：2009-03-20 00:00:00
