Regulation of armadillo by a Drosophila APC inhibits neuronal apoptosis during retinal development.


:We find that inactivation of a Drosophila homolog of the tumor suppressor APC (D-APC) causes retinal neuronal degeneration and pigment cell hypertrophy, a phenotype remarkably similar to that found in humans with germline APC mutations. Retinal degeneration in the D-APC mutant results from apoptotic cell death, which accompanies a defect in neuronal differentiation. Reduction in the Drosophila beta-catenin, Armadillo (Arm), rescues the differentiation defect and prevents apoptosis in the D-APC mutant, while Arm overexpression mimics D-APC inactivation. A mutation in dTCF, the DNA-binding protein required in Arm-mediated signal transduction, can eliminate the cell death without rescuing the differentiation defect in D-APC mutants. Uncoupling of these two Arm-induced processes suggests a novel role for the Arm/dTCF complex in the activation of apoptosis.






Ahmed Y,Hayashi S,Levine A,Wieschaus E




Has Abstract


1998-06-26 00:00:00
















  • The structure of ClpB: a molecular chaperone that rescues proteins from an aggregated state.

    abstract::Molecular chaperones assist protein folding by facilitating their "forward" folding and preventing aggregation. However, once aggregates have formed, these chaperones cannot facilitate protein disaggregation. Bacterial ClpB and its eukaryotic homolog Hsp104 are essential proteins of the heat-shock response, which have...


    pub_type: 杂志文章


    authors: Lee S,Sowa ME,Watanabe YH,Sigler PB,Chiu W,Yoshida M,Tsai FT

    更新日期:2003-10-17 00:00:00

  • RNase III cleaves eukaryotic preribosomal RNA at a U3 snoRNP-dependent site.

    abstract::A yeast gene homologous to bacterial RNase III (RNT1) encodes a double-strand-specific endoribonuclease essential for ribosome synthesis. Two rRNA processing events are blocked in cells temperature sensitive for RNT1: cleavage at the snoRNA-dependent AO site in the 5' ETS and cleavage in the 3' ETS. Recombinant RNT1 p...


    pub_type: 杂志文章


    authors: Elela SA,Igel H,Ares M Jr

    更新日期:1996-04-05 00:00:00

  • An Engineered CRISPR-Cas9 Mouse Line for Simultaneous Readout of Lineage Histories and Gene Expression Profiles in Single Cells.

    abstract::Tracing the lineage history of cells is key to answering diverse and fundamental questions in biology. Coupling of cell ancestry information with other molecular readouts represents an important goal in the field. Here, we describe the CRISPR array repair lineage tracing (CARLIN) mouse line and corresponding analysis ...


    pub_type: 杂志文章


    authors: Bowling S,Sritharan D,Osorio FG,Nguyen M,Cheung P,Rodriguez-Fraticelli A,Patel S,Yuan WC,Fujiwara Y,Li BE,Orkin SH,Hormoz S,Camargo FD

    更新日期:2020-06-11 00:00:00

  • Back to the future with ubiquitin.

    abstract::Two papers published in 1984 by the Varshavsky laboratory revealed that the ubiquitin/proteasome pathway is the principal system for degradation of short-lived proteins in mammalian cells, setting the stage for future demonstrations of this pathway's many regulatory roles. This perspective discusses the impact of thos...


    pub_type: 评论,杂志文章,评审


    authors: Pickart CM

    更新日期:2004-01-23 00:00:00

  • Expression of rRNA and tRNA genes in Escherichia coli: evidence for feedback regulation by products of rRNA operons.

    abstract::We have tested a model for global ribosome biosynthesis by examining the effects of increased gene dosage on the synthesis of rRNA. Increasing gene dosage does not lead to a significant increase in total rRNA transcription; i.e., rRNA synthesis from individual rRNA operons is reduced to keep total rRNA production unch...


    pub_type: 杂志文章


    authors: Jinks-Robertson S,Gourse RL,Nomura M

    更新日期:1983-07-01 00:00:00

  • A Smad transcriptional corepressor.

    abstract::Following TGFbeta receptor-mediated phosphorylation and association with Smad4, Smad2 moves into the nucleus, binds to target promoters in association with DNA-binding cofactors, and recruits coactivators such as p300/CBP to activate transcription. We identified the homeodomain protein TGIF as a Smad2-binding protein ...


    pub_type: 杂志文章


    authors: Wotton D,Lo RS,Lee S,Massagué J

    更新日期:1999-04-02 00:00:00

  • A role for a small stable RNA in modulating the activity of DNA-binding proteins.

    abstract::The 10Sa RNA, encoded by the E. coli ssrA gene, appears to modulate action of some DNA-binding proteins. When ssrA is inactivated, lacZ expression from the lac operon, as well as galK from a gal operon fused to a phage lambda promoter, is reduced from that observed in bacteria wild-type for ssrA. These differences are...


    pub_type: 杂志文章


    authors: Retallack DM,Friedman DI

    更新日期:1995-10-20 00:00:00

  • Identification of a regulated pathway for nuclear pre-mRNA turnover.

    abstract::We have identified a nuclear pathway that rapidly degrades unspliced pre-mRNAs in yeast. This involves 3'-->5' degradation by the exosome complex and 5'-->3' degradation by the exonuclease Rat1p. 3'-->5' degradation is normally the major pathway and is regulated in response to carbon source. Inhibition of pre-mRNA deg...


    pub_type: 杂志文章


    authors: Bousquet-Antonelli C,Presutti C,Tollervey D

    更新日期:2000-09-15 00:00:00

  • Procollagen and collagen produced by a teratocarcinoma-derived cell line, TSD4: evidence for a new molecular form of collagen.

    abstract::Procollagen and collagen were isolated from the culture medium and cell layer of line TSD4 (obtained from mouse teratocarcinoma OTT6050). SDS-polyacrylamide gel electrophoresis of the highly purified procollagen fraction demonstrated that the fraction is composed of theta chains (150,000 daltons), pro alpha chains (13...


    pub_type: 杂志文章


    authors: Little CD,Church RL,Miller RA,Ruddle FH

    更新日期:1977-02-01 00:00:00

  • Evaluating the Effects of SARS-CoV-2 Spike Mutation D614G on Transmissibility and Pathogenicity.

    abstract::Global dispersal and increasing frequency of the SARS-CoV-2 spike protein variant D614G are suggestive of a selective advantage but may also be due to a random founder effect. We investigate the hypothesis for positive selection of spike D614G in the United Kingdom using more than 25,000 whole genome SARS-CoV-2 sequen...


    pub_type: 杂志文章


    authors: Volz E,Hill V,McCrone JT,Price A,Jorgensen D,O'Toole Á,Southgate J,Johnson R,Jackson B,Nascimento FF,Rey SM,Nicholls SM,Colquhoun RM,da Silva Filipe A,Shepherd J,Pascall DJ,Shah R,Jesudason N,Li K,Jarrett R,Pacchi

    更新日期:2021-01-07 00:00:00

  • A Species-Wide Inventory of NLR Genes and Alleles in Arabidopsis thaliana.

    abstract::Infectious disease is both a major force of selection in nature and a prime cause of yield loss in agriculture. In plants, disease resistance is often conferred by nucleotide-binding leucine-rich repeat (NLR) proteins, intracellular immune receptors that recognize pathogen proteins and their effects on the host. Consi...


    pub_type: 杂志文章


    authors: Van de Weyer AL,Monteiro F,Furzer OJ,Nishimura MT,Cevik V,Witek K,Jones JDG,Dangl JL,Weigel D,Bemm F

    更新日期:2019-08-22 00:00:00

  • Deletion of DDB1 in mouse brain and lens leads to p53-dependent elimination of proliferating cells.

    abstract::DDB1, a component of the Cul4 ubiquitin ligase complex, promotes protein ubiquitination in diverse cellular functions, including nuclear excision repair, regulation of the cell cycle, and DNA replication. To investigate its physiological significance, we generated mice with null and floxed alleles of the DDB1 gene. He...


    pub_type: 杂志文章


    authors: Cang Y,Zhang J,Nicholas SA,Bastien J,Li B,Zhou P,Goff SP

    更新日期:2006-12-01 00:00:00

  • An Akt/beta-arrestin 2/PP2A signaling complex mediates dopaminergic neurotransmission and behavior.

    abstract::Dopamine plays an important role in the etiology of schizophrenia, and D2 class dopamine receptors are the best-established target of antipsychotic drugs. Here we show that D2 class-receptor-mediated Akt regulation involves the formation of signaling complexes containing beta-arrestin 2, PP2A, and Akt. beta-arrestin 2...


    pub_type: 杂志文章


    authors: Beaulieu JM,Sotnikova TD,Marion S,Lefkowitz RJ,Gainetdinov RR,Caron MG

    更新日期:2005-07-29 00:00:00

  • Fast-Spiking Interneurons Supply Feedforward Control of Bursting, Calcium, and Plasticity for Efficient Learning.

    abstract::Fast-spiking interneurons (FSIs) are a prominent class of forebrain GABAergic cells implicated in two seemingly independent network functions: gain control and network plasticity. Little is known, however, about how these roles interact. Here, we use a combination of cell-type-specific ablation, optogenetics, electrop...


    pub_type: 杂志文章


    authors: Owen SF,Berke JD,Kreitzer AC

    更新日期:2018-02-08 00:00:00

  • Single-Cell Transcriptomics Reveals Early Emergence of Liver Parenchymal and Non-parenchymal Cell Lineages.

    abstract::The cellular complexity and scale of the early liver have constrained analyses examining its emergence during organogenesis. To circumvent these issues, we analyzed 45,334 single-cell transcriptomes from embryonic day (E)7.5, when endoderm progenitors are specified, to E10.5 liver, when liver parenchymal and non-paren...


    pub_type: 杂志文章


    authors: Lotto J,Drissler S,Cullum R,Wei W,Setty M,Bell EM,Boutet SC,Nowotschin S,Kuo YY,Garg V,Pe'er D,Church DM,Hadjantonakis AK,Hoodless PA

    更新日期:2020-10-29 00:00:00

  • Cancer-associated protein kinase C mutations reveal kinase's role as tumor suppressor.

    abstract::Protein kinase C (PKC) isozymes have remained elusive cancer targets despite the unambiguous tumor promoting function of their potent ligands, phorbol esters, and the prevalence of their mutations. We analyzed 8% of PKC mutations identified in human cancers and found that, surprisingly, most were loss of function and ...


    pub_type: 杂志文章


    authors: Antal CE,Hudson AM,Kang E,Zanca C,Wirth C,Stephenson NL,Trotter EW,Gallegos LL,Miller CJ,Furnari FB,Hunter T,Brognard J,Newton AC

    更新日期:2015-01-29 00:00:00

  • Transcription of specific genes in isolated nuclei by exogenous RNA polymerases.

    abstract::Mouse plasmacytoma (MOPC) 460 cells contain two chromatographic forms of RNA polymerase III (IIIA and IIIB) in addition to the major class I and II RNA polymerases. Nuclei isolated from these cells actively synthesize RNA. Among the discrete transcription products observed are the 5S and 4.5S RNAs and additional low m...


    pub_type: 杂志文章


    authors: Sklar VE,Roeder RG

    更新日期:1977-03-01 00:00:00

  • A ceRNA hypothesis: the Rosetta Stone of a hidden RNA language?

    abstract::Here, we present a unifying hypothesis about how messenger RNAs, transcribed pseudogenes, and long noncoding RNAs "talk" to each other using microRNA response elements (MREs) as letters of a new language. We propose that this "competing endogenous RNA" (ceRNA) activity forms a large-scale regulatory network across the...


    pub_type: 杂志文章


    authors: Salmena L,Poliseno L,Tay Y,Kats L,Pandolfi PP

    更新日期:2011-08-05 00:00:00

  • Initiating cellular stress responses.

    abstract::The phosphoinositide 3-kinase related kinases (PIKKs) mediate responses to diverse stresses, including DNA double-strand breaks (DSBs), abnormal replication fork progression, the recognition of premature termination codons in mRNAs, and inadequate nutrient availability. Rigorous control of these kinases limits cellula...


    pub_type: 杂志文章,评审


    authors: Bakkenist CJ,Kastan MB

    更新日期:2004-07-09 00:00:00

  • Reprogramming of DNA methylation in pollen guides epigenetic inheritance via small RNA.

    abstract::Epigenetic inheritance is more widespread in plants than in mammals, in part because mammals erase epigenetic information by germline reprogramming. We sequenced the methylome of three haploid cell types from developing pollen: the sperm cell, the vegetative cell, and their precursor, the postmeiotic microspore, and f...


    pub_type: 杂志文章


    authors: Calarco JP,Borges F,Donoghue MT,Van Ex F,Jullien PE,Lopes T,Gardner R,Berger F,Feijó JA,Becker JD,Martienssen RA

    更新日期:2012-09-28 00:00:00

  • Posttranscriptional control of T cell effector function by aerobic glycolysis.

    abstract::A "switch" from oxidative phosphorylation (OXPHOS) to aerobic glycolysis is a hallmark of T cell activation and is thought to be required to meet the metabolic demands of proliferation. However, why proliferating cells adopt this less efficient metabolism, especially in an oxygen-replete environment, remains incomplet...


    pub_type: 杂志文章


    authors: Chang CH,Curtis JD,Maggi LB Jr,Faubert B,Villarino AV,O'Sullivan D,Huang SC,van der Windt GJ,Blagih J,Qiu J,Weber JD,Pearce EJ,Jones RG,Pearce EL

    更新日期:2013-06-06 00:00:00

  • Tumor Evolution: A Problem of Histocompatibility.

    abstract::In this issue of Cell, two articles show that tumor-specific changes in HLA-mediated antigen presentation affect tumor immunogenicity and may play a role in shaping cancer cell survival. ...


    pub_type: 评论,杂志文章


    authors: Vokes NI,Van Allen EM

    更新日期:2017-11-30 00:00:00

  • Meiosis-specific formation of joint DNA molecules containing sequences from homologous chromosomes.

    abstract::All recombination models postulate one or more recombination intermediates that are joint molecules containing two homologous parental molecules. A spike of branched DNA molecules not seen in DNA from mitotic cells was found in the two-dimensional gel analysis of meiotic DNA from S. cerevisiae. The mass of molecules i...


    pub_type: 杂志文章


    authors: Collins I,Newlon CS

    更新日期:1994-01-14 00:00:00

  • Making Sense of Mutation: What D614G Means for the COVID-19 Pandemic Remains Unclear.

    abstract::In this issue of Cell, Korber et al. found that a SARS-CoV-2 variant in the spike protein D614G rapidly became dominant around the world. Although clinical and in vitro data suggest that D614G changes the virus phenotype, the impact of the mutation on transmission, disease, and vaccine and therapeutic development are ...


    pub_type: 评论,杂志文章


    authors: Grubaugh ND,Hanage WP,Rasmussen AL

    更新日期:2020-08-20 00:00:00

  • Mutations of immunoglobulin transmembrane and cytoplasmic domains: effects on intracellular signaling and antigen presentation.

    abstract::The membrane-bound form of immunoglobulin serves as an antigen-specific receptor for B cells mediating signal transduction and antigen presentation. We have developed an assay that reconstitutes both these physiologic responses with respect to the antigen phosphorylcholine. By introducing specific mutations in the hum...


    pub_type: 杂志文章


    authors: Shaw AC,Mitchell RN,Weaver YK,Campos-Torres J,Abbas AK,Leder P

    更新日期:1990-10-19 00:00:00

  • Translation/secretion coupling by type III secretion systems.

    abstract::Type III secretion systems mediate export of virulence proteins and flagellar assembly subunits in Gram-negative bacteria. Chaperones specific to each class of secreted protein are believed to prevent degradation of the secreted substrates. We show that an additional role of chaperones may be to regulate translation o...


    pub_type: 杂志文章


    authors: Karlinsey JE,Lonner J,Brown KL,Hughes KT

    更新日期:2000-08-18 00:00:00

  • E. coli mutant pleiotropically defective in the export of secreted proteins.

    abstract::A hybrid beta-galactosidase molecule containing a substantial portion of the amino-terminal sequence of the maltose-binding protein is inserted in the cytoplasmic membrane of E. coli; in this location, the protein has very low enzymatic activity. The strain producing it is, therefore, Lac-. Selection for derivatives o...


    pub_type: 杂志文章


    authors: Oliver DB,Beckwith J

    更新日期:1981-09-01 00:00:00

  • Preview. MicroRNAs: from decay to decoy.

    abstract::MicroRNAs interact with Argonaute proteins to guide posttranscriptional gene silencing. Eiring et al. (2010) now show that miR-328 has a second function, acting as a decoy by binding to hnRNP E2 and lifting its translational repression of an mRNA involved in myeloid cell differentiation. ...


    pub_type: 评论,杂志文章


    authors: Beitzinger M,Meister G

    更新日期:2010-03-05 00:00:00

  • Transmembrane location of oligosaccharide-lipid synthesis in microsomal vesicles.

    abstract::The oligosaccharide-lipid which is the precursor of asparagine-linked oligosaccharides of eucaryotic glycoproteins is synthesized from sugar nucleotides in the endoplasmic reticulum. The transmembrane location of the assembly of this oligosaccharide-lipid has been studied in vitro in rat liver microsomes. Protease tre...


    pub_type: 杂志文章


    authors: Snider MD,Sultzman LA,Robbins PW

    更新日期:1980-09-01 00:00:00

  • Crystal structure of the A domain from the alpha subunit of integrin CR3 (CD11b/CD18).

    abstract::We have determined the high resolution crystal structure of the A domain from the alpha chain of integrin CR3. The domain adopts a classic alpha/beta "Rossmann" fold and contains an unusual Mg2+ coordination site at its surface. One of the coordinating ligands is the glutamate side chain from another A domain molecule...


    pub_type: 杂志文章


    authors: Lee JO,Rieu P,Arnaout MA,Liddington R

    更新日期:1995-02-24 00:00:00