Regulation of armadillo by a Drosophila APC inhibits neuronal apoptosis during retinal development.


:We find that inactivation of a Drosophila homolog of the tumor suppressor APC (D-APC) causes retinal neuronal degeneration and pigment cell hypertrophy, a phenotype remarkably similar to that found in humans with germline APC mutations. Retinal degeneration in the D-APC mutant results from apoptotic cell death, which accompanies a defect in neuronal differentiation. Reduction in the Drosophila beta-catenin, Armadillo (Arm), rescues the differentiation defect and prevents apoptosis in the D-APC mutant, while Arm overexpression mimics D-APC inactivation. A mutation in dTCF, the DNA-binding protein required in Arm-mediated signal transduction, can eliminate the cell death without rescuing the differentiation defect in D-APC mutants. Uncoupling of these two Arm-induced processes suggests a novel role for the Arm/dTCF complex in the activation of apoptosis.






Ahmed Y,Hayashi S,Levine A,Wieschaus E




Has Abstract


1998-06-26 00:00:00
















  • Targeted disruption of the trkB neurotrophin receptor gene results in nervous system lesions and neonatal death.

    abstract::We have generated mice carrying a germline mutation in the tyrosine kinase catalytic domain of the trkB gene. This mutation eliminates expression of gp145trkB, a protein-tyrosine kinase that serves as the signaling receptor for two members of the nerve growth factor family of neurotrophins, brain-derived neurotrophic ...


    pub_type: 杂志文章


    authors: Klein R,Smeyne RJ,Wurst W,Long LK,Auerbach BA,Joyner AL,Barbacid M

    更新日期:1993-10-08 00:00:00

  • Point mutations identify the conserved, intron-contained TACTAAC box as an essential splicing signal sequence in yeast.

    abstract::Our previous deletion experiments have shown that a short region of yeast nuclear gene introns containing the conserved sequence 5'-TACTAACA-3' is essential for splicing. In this report we show that the chemically synthesized decanucleotide 5'-TGTACTAACA-3', when introduced into a hybrid gene forming unspliceable RNA ...


    pub_type: 杂志文章


    authors: Langford CJ,Klinz FJ,Donath C,Gallwitz D

    更新日期:1984-03-01 00:00:00

  • Structure of the catalytic domain of human DOT1L, a non-SET domain nucleosomal histone methyltransferase.

    abstract::Dot1 is an evolutionarily conserved histone methyltransferase that methylates lysine-79 of histone H3 in the core domain. Unlike other histone methyltransferases, Dot1 does not contain a SET domain, and it specifically methylates nucleosomal histone H3. We have solved a 2.5 A resolution structure of the catalytic doma...


    pub_type: 杂志文章


    authors: Min J,Feng Q,Li Z,Zhang Y,Xu RM

    更新日期:2003-03-07 00:00:00

  • Crystal structure of human uracil-DNA glycosylase in complex with a protein inhibitor: protein mimicry of DNA.

    abstract::Uracil-DNA glycosylase inhibitor (Ugi) is a B. subtilis bacteriophage protein that protects the uracil-containing phage DNA by irreversibly inhibiting the key DNA repair enzyme uracil-DNA glycosylase (UDG). The 1.9 A crystal structure of Ugi complexed to human UDG reveals that the Ugi structure, consisting of a twiste...


    pub_type: 杂志文章


    authors: Mol CD,Arvai AS,Sanderson RJ,Slupphaug G,Kavli B,Krokan HE,Mosbaugh DW,Tainer JA

    更新日期:1995-09-08 00:00:00

  • The protein network of HIV budding.

    abstract::HIV release requires TSG101, a cellular factor that sorts proteins into vesicles that bud into multivesicular bodies (MVB). To test whether other proteins involved in MVB biogenesis (the class E proteins) also participate in HIV release, we identified 22 candidate human class E proteins. These proteins were connected ...


    pub_type: 杂志文章


    authors: von Schwedler UK,Stuchell M,Müller B,Ward DM,Chung HY,Morita E,Wang HE,Davis T,He GP,Cimbora DM,Scott A,Kräusslich HG,Kaplan J,Morham SG,Sundquist WI

    更新日期:2003-09-19 00:00:00

  • Crystal structures of T. brucei MRP1/MRP2 guide-RNA binding complex reveal RNA matchmaking mechanism.

    abstract::The mitochondrial RNA binding proteins MRP1 and MRP2 form a heteromeric complex that functions in kinetoplastid RNA editing. In this process, MRP1/MRP2 serves as a matchmaker by binding to guide RNAs and facilitating their hybridization with cognate preedited mRNAs. To understand the mechanism by which this complex pe...


    pub_type: 杂志文章


    authors: Schumacher MA,Karamooz E,Zíková A,Trantírek L,Lukes J

    更新日期:2006-08-25 00:00:00

  • Transcription termination within the E1A gene of adenovirus induced by insertion of the mouse beta-major globin terminator element.

    abstract::In induced erythroleukemia cells, transcription of the beta-globin gene terminates in a region 600-1500 nucleotides downstream of the poly(A) site. To determine whether this region of the mouse DNA functions to terminate transcription when moved to another genomic site, portions of the putative termination region have...


    pub_type: 杂志文章


    authors: Falck-Pedersen E,Logan J,Shenk T,Darnell JE Jr

    更新日期:1985-04-01 00:00:00

  • Genomic rearrangements in a mouse cell line containing integrated SV40 DNA.

    abstract::In the SV40-transformed mouse embryo fibroblast cell line SVT2/S, genomic rearrangements involving the SV40 DNA and flanking host sequences were identified by Southern blot hybridization using viral DNA as probe. No rearrangements of SV40 DNA integrated into nonpermissive mouse cells have been previously described. Th...


    pub_type: 杂志文章


    authors: Sager R,Anisowicz A,Howell N

    更新日期:1981-01-01 00:00:00

  • Signal peptides open protein-conducting channels in E. coli.

    abstract::Plasma membrane vesicles and protoplasts of Escherichia coli were fused to planar lipid bilayers and studied with electrophysiological techniques. Large transmembrane aqueous channels were opened when 0.2 nM LamB signal peptide was added to the cytoplasmic side of the membrane. These aqueous pores are similar in condu...


    pub_type: 杂志文章


    authors: Simon SM,Blobel G

    更新日期:1992-05-15 00:00:00

  • The Antagonistic Gene Paralogs Upf3a and Upf3b Govern Nonsense-Mediated RNA Decay.

    abstract::Gene duplication is a major evolutionary force driving adaptation and speciation, as it allows for the acquisition of new functions and can augment or diversify existing functions. Here, we report a gene duplication event that yielded another outcome--the generation of antagonistic functions. One product of this dupli...


    pub_type: 杂志文章


    authors: Shum EY,Jones SH,Shao A,Dumdie J,Krause MD,Chan WK,Lou CH,Espinoza JL,Song HW,Phan MH,Ramaiah M,Huang L,McCarrey JR,Peterson KJ,De Rooij DG,Cook-Andersen H,Wilkinson MF

    更新日期:2016-04-07 00:00:00

  • Selective Chemical Inhibition of PGC-1α Gluconeogenic Activity Ameliorates Type 2 Diabetes.

    abstract::Type 2 diabetes (T2D) is a worldwide epidemic with a medical need for additional targeted therapies. Suppression of hepatic glucose production (HGP) effectively ameliorates diabetes and can be exploited for its treatment. We hypothesized that targeting PGC-1α acetylation in the liver, a chemical modification known to ...


    pub_type: 杂志文章


    authors: Sharabi K,Lin H,Tavares CDJ,Dominy JE,Camporez JP,Perry RJ,Schilling R,Rines AK,Lee J,Hickey M,Bennion M,Palmer M,Nag PP,Bittker JA,Perez J,Jedrychowski MP,Ozcan U,Gygi SP,Kamenecka TM,Shulman GI,Schreiber SL,Gr

    更新日期:2017-03-23 00:00:00

  • Ceramide synthase mediates daunorubicin-induced apoptosis: an alternative mechanism for generating death signals.

    abstract::The sphingomyelin pathway, which is initiated by sphingomyelin hydrolysis to generate the second messenger ceramide, signals apoptosis for tumor necrosis factor alpha, Fas, and ionizing radiation. In the present studies, the anticancer drug daunorubicin also stimulated ceramide elevation and apoptosis in P388 and U937...


    pub_type: 杂志文章


    authors: Bose R,Verheij M,Haimovitz-Friedman A,Scotto K,Fuks Z,Kolesnick R

    更新日期:1995-08-11 00:00:00

  • Molecular cloning and functional analysis of Drosophila TAF110 reveal properties expected of coactivators.

    abstract::The general transcription factor TFIID is a multiprotein complex containing the TATA-binding protein and several associated factors (TAFs), some of which may function as coactivators that are essential for activated, but not basal, transcription. Here we describe the isolation and characterization of the first gene en...


    pub_type: 杂志文章


    authors: Hoey T,Weinzierl RO,Gill G,Chen JL,Dynlacht BD,Tjian R

    更新日期:1993-01-29 00:00:00

  • Cryo-EM Structure of the Human Cannabinoid Receptor CB2-Gi Signaling Complex.

    abstract::Drugs selectively targeting CB2 hold promise for treating neurodegenerative disorders, inflammation, and pain while avoiding psychotropic side effects mediated by CB1. The mechanisms underlying CB2 activation and signaling are poorly understood but critical for drug design. Here we report the cryo-EM structure of the ...


    pub_type: 杂志文章


    authors: Xing C,Zhuang Y,Xu TH,Feng Z,Zhou XE,Chen M,Wang L,Meng X,Xue Y,Wang J,Liu H,McGuire TF,Zhao G,Melcher K,Zhang C,Xu HE,Xie XQ

    更新日期:2020-02-20 00:00:00

  • Cowpea mosaic virus RNAs have neither m7GpppN ... nor mono-, di- or triphosphates at their 5' ends.

    abstract::32P-labeled cowpea mosaic virus (CPMV) RNAs were analyzed for their 5' termini by three different fingerprinting standard procedures. These analyses showed unequivocally that CPMV RNAs lack a structure like m7GpppN ... at their 5' termini. In addition, neither mono-, di- or triphosphorylated [(p)(p)pN ....] termini we...


    pub_type: 杂志文章


    authors: Klootwijk J,Klein I,Zabel P,van Kammen A

    更新日期:1977-05-01 00:00:00

  • A secondary attachment site for bacteriophage lambda in trpC of E. coli.

    abstract::We have determined the nucleotide sequence of a secondary lambda attachment site in trpC. Direct sequence analysis of lambdatrp transducing phage DNA fragments carrying the two prophage attachment sites reveals a 6 nucleotide homology in the crossover region which is a subset of the 15 nucleotide core sequence in the ...


    pub_type: 杂志文章


    authors: Christie GE,Platt T

    更新日期:1979-02-01 00:00:00

  • Release of low density lipoprotein from its cell surface receptor by sulfated glycosaminoglycans.

    abstract::The sulfated glycosaminoglycan, heparin, was found to release 125I-labeled low density lipoprotein (125I-LDL) from its receptor site on the surface of normal human fibroblasts. Measurement of the amount of 125I-LDL released by heparin permitted the resolution of the total cellular uptake of 125I-LDL at 37 degrees C in...


    pub_type: 杂志文章


    authors: Goldstein JL,Basu SK,Brunschede GY,Brown MS

    更新日期:1976-01-01 00:00:00

  • Family of proteins that interact with TFIID and regulate promoter activity.

    abstract::A family of proteins was shown to bind cooperatively with TFIID to core promoters, as previously demonstrated for the general initiation factor TFIIA. These factors form distinct complexes with TFIID, fail to bind DNA in the absence of TFIID, differ chromatographically from TFIIA, and compete with TFIIA for binding to...


    pub_type: 杂志文章


    authors: Meisterernst M,Roeder RG

    更新日期:1991-11-01 00:00:00

  • Regulatory Expansion in Mammals of Multivalent hnRNP Assemblies that Globally Control Alternative Splicing.

    abstract::Alternative splicing (AS) patterns have diverged rapidly during vertebrate evolution, yet the functions of most species- and lineage-specific splicing events are not known. We observe that mammalian-specific AS events are enriched in transcript sequences encoding intrinsically disordered regions (IDRs) of proteins, in...


    pub_type: 杂志文章


    authors: Gueroussov S,Weatheritt RJ,O'Hanlon D,Lin ZY,Narula A,Gingras AC,Blencowe BJ

    更新日期:2017-07-13 00:00:00

  • Mutant p53 gain of function in two mouse models of Li-Fraumeni syndrome.

    abstract::The p53 tumor suppressor gene is commonly altered in human tumors, predominantly through missense mutations that result in accumulation of mutant p53 protein. These mutations may confer dominant-negative or gain-of-function properties to p53. To ascertain the physiological effects of p53 point mutation, the structural...


    pub_type: 杂志文章


    authors: Olive KP,Tuveson DA,Ruhe ZC,Yin B,Willis NA,Bronson RT,Crowley D,Jacks T

    更新日期:2004-12-17 00:00:00

  • Metabolic Control of Longevity.

    abstract::Several metabolic alterations accumulate over time along with a reduction in biological fitness, suggesting the existence of a "metabolic clock" that controls aging. Multiple inborn defects in metabolic circuitries accelerate aging, whereas genetic loci linked to exceptional longevity influence metabolism. Each of the...


    pub_type: 杂志文章,评审


    authors: López-Otín C,Galluzzi L,Freije JMP,Madeo F,Kroemer G

    更新日期:2016-08-11 00:00:00

  • Corticoamygdala Transfer of Socially Derived Information Gates Observational Learning.

    abstract::Observational learning is a powerful survival tool allowing individuals to learn about threat-predictive stimuli without directly experiencing the pairing of the predictive cue and punishment. This ability has been linked to the anterior cingulate cortex (ACC) and the basolateral amygdala (BLA). To investigate how inf...


    pub_type: 杂志文章


    authors: Allsop SA,Wichmann R,Mills F,Burgos-Robles A,Chang CJ,Felix-Ortiz AC,Vienne A,Beyeler A,Izadmehr EM,Glober G,Cum MI,Stergiadou J,Anandalingam KK,Farris K,Namburi P,Leppla CA,Weddington JC,Nieh EH,Smith AC,Ba D,Bro

    更新日期:2018-05-31 00:00:00

  • At least 104 nucleotides are transposed from the 5' terminus of the avian sarcoma virus genome to the 5' termini of smaller viral mRNAs.

    abstract::Cells producing avian sarcoma virus (ASV) contain at least three virus-specific mRNAs, two of which are encoded within the 3' half of the viral genome. Each of these viral RNAs can hybridize with single-stranded DNA(cDNA5') that is complementary to a sequence of 101 nucleotides found at the 5' terminus of the ASV geno...


    pub_type: 杂志文章


    authors: Cordell B,Weiss SR,Varmus HE,Bishop JM

    更新日期:1978-09-01 00:00:00

  • Exported proteins required for virulence and rigidity of Plasmodium falciparum-infected human erythrocytes.

    abstract::A major part of virulence for Plasmodium falciparum malaria infection, the most lethal parasitic disease of humans, results from increased rigidity and adhesiveness of infected host red cells. These changes are caused by parasite proteins exported to the erythrocyte using novel trafficking machinery assembled in the h...


    pub_type: 杂志文章


    authors: Maier AG,Rug M,O'Neill MT,Brown M,Chakravorty S,Szestak T,Chesson J,Wu Y,Hughes K,Coppel RL,Newbold C,Beeson JG,Craig A,Crabb BS,Cowman AF

    更新日期:2008-07-11 00:00:00

  • Human IL-3 (multi-CSF): identification by expression cloning of a novel hematopoietic growth factor related to murine IL-3.

    abstract::A cDNA clone encoding a novel hematopoietic growth factor activity produced by a gibbon T cell line has been identified using a mammalian cell expression cloning system. The sequence of this cDNA proved to have significant homology to the sequence encoding murine interleukin 3 (IL-3). The human gene, which was readily...


    pub_type: 杂志文章


    authors: Yang YC,Ciarletta AB,Temple PA,Chung MP,Kovacic S,Witek-Giannotti JS,Leary AC,Kriz R,Donahue RE,Wong GG

    更新日期:1986-10-10 00:00:00

  • Eucaryotic transposable genetic elements with inverted terminal repeats.

    abstract::DNA carrying inverted repeats was tested for transposition within the Drosophila genome. Five Bam HI segments containing related inverted repeats were isolated from D. melanogaster and analyzed by electron microscopy and restriction mapping. Southern blot experiments using single-copy flanking sequences as probes allo...


    pub_type: 杂志文章


    authors: Potter S,Truett M,Phillips M,Maher A

    更新日期:1980-07-01 00:00:00

  • Probing the Diversity of T Cell Dysfunction in Cancer.

    abstract::T cell dysfunction in cancer comes in many forms, with two new varieties reported in this issue. Daley et al. find that T cells expressing γδ T cell receptors (TCR) promote pancreatic tumor growth by inhibiting activation of T cells with conventional TCRs. Singer et al. characterize dysfunctional tumor infiltrating ly...


    pub_type: 评论,杂志文章


    authors: Sowell RT,Kaech SM

    更新日期:2016-09-08 00:00:00

  • A role for hematopoietic stem cells in promoting angiogenesis.

    abstract::Angiogenesis is an important event for embryonic organogenesis as well as for tissue repair in the adult. Here, we show that hematopoietic stem cells (HSCs) play important roles for angiogenesis during embryogenesis. To investigate the role of HSCs in endothelial cell (EC) development, we analyzed AML1-deficient embry...


    pub_type: 杂志文章


    authors: Takakura N,Watanabe T,Suenobu S,Yamada Y,Noda T,Ito Y,Satake M,Suda T

    更新日期:2000-07-21 00:00:00

  • Centrosomes, and not nuclei, initiate pole cell formation in Drosophila embryos.

    abstract::An injection of aphidicolin into early Drosophila embryos inhibits DNA synthesis and nuclear division, while centrosome replication and many other aspects of the mitotic cycle continue. If aphidicolin is injected at nuclear cycle 7-8, the normal migration of nuclei to the embryo cortex is completely inhibited. In most...


    pub_type: 杂志文章


    authors: Raff JW,Glover DM

    更新日期:1989-05-19 00:00:00

  • Molecular Diversity of Midbrain Development in Mouse, Human, and Stem Cells.

    abstract::Understanding human embryonic ventral midbrain is of major interest for Parkinson's disease. However, the cell types, their gene expression dynamics, and their relationship to commonly used rodent models remain to be defined. We performed single-cell RNA sequencing to examine ventral midbrain development in human and ...


    pub_type: 杂志文章


    authors: La Manno G,Gyllborg D,Codeluppi S,Nishimura K,Salto C,Zeisel A,Borm LE,Stott SRW,Toledo EM,Villaescusa JC,Lönnerberg P,Ryge J,Barker RA,Arenas E,Linnarsson S

    更新日期:2016-10-06 00:00:00