Abstract：
:The relationship between activation of resting chick embryo fibroblasts by proteases and proteolytic alteration of the cell surface has been investigated. Five different proteases were examined: trypsin, collagenase, plasmin, alpha-chymotrypsin, and thrombin. All of these proteases, when added to the culture medium at concentrations of 0.08-2.2 mug/ml, stimulated deoxyglucose uptake and induced cell division. The absolute levels of stimulation depended on the specific protease. Activation ranged from a doubling in cell number in 24 hr for trypsin and thrombin down to a 47% increase in cell number for alpha-chymotrypsin. Except in the case of thrombin, the stimulatory effects of these proteases correlated with breakdown of Z, a protein which is the major chick surface protein as revealed by lactoperoxidase-catalyzed iodination and which disappears upon transformation. In the case of thrombin, stimulatory concentrations brought about no detectable loss of surface components. Thus loss of Z is not a necessary condition for activation of chick fibroblasts; it may be a sufficient condition for activation of part of the cell population.
journal_name
Celljournal_title
Cellauthors
Blumberg PM,Robbins PWdoi
10.1016/0092-8674(75)90004-5subject
Has Abstractpub_date
1975-10-01 00:00:00pages
137-47issue
2eissn
0092-8674issn
1097-4172pii
0092-8674(75)90004-5journal_volume
6pub_type
杂志文章相关文献
CELL文献大全abstract：:Membrane scission is essential for intracellular trafficking. While BAR domain proteins such as endophilin have been reported in dynamin-independent scission of tubular membrane necks, the cutting mechanism has yet to be deciphered. Here, we combine a theoretical model, in vitro, and in vivo experiments revealing how ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.05.047
更新日期：2017-06-29 00:00:00
abstract：:Chronic viral infections represent a major burden to human health, and modulation of the immune system is emerging as a novel approach to fighting such infections. Pellegrini et al. (2011) demonstrate that treatment with the cytokine IL-7 may reinvigorate the immune response to persistent infection by targeting immuno...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2011.01.038
更新日期：2011-02-18 00:00:00
abstract：:Cellular functioning requires the orchestration of thousands of molecular interactions in time and space. Yet most molecules in a cell move by diffusion, which is sensitive to external factors like temperature. How cells sustain complex, diffusion-based systems across wide temperature ranges is unknown. Here, we uncov...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2020.10.017
更新日期：2020-12-10 00:00:00
abstract：:There are still no effective treatments to prevent, halt, or reverse Alzheimer's disease, but research advances over the past three decades could change this gloomy picture. Genetic studies demonstrate that the disease has multiple causes. Interdisciplinary approaches combining biochemistry, molecular and cell biology...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2012.02.040
更新日期：2012-03-16 00:00:00
abstract：:We have identified a nuclear pathway that rapidly degrades unspliced pre-mRNAs in yeast. This involves 3'-->5' degradation by the exosome complex and 5'-->3' degradation by the exonuclease Rat1p. 3'-->5' degradation is normally the major pathway and is regulated in response to carbon source. Inhibition of pre-mRNA deg...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)00065-9
更新日期：2000-09-15 00:00:00
abstract：:During early Drosophila embryogenesis, several zygotic gene products act to establish a posttranscriptional activity gradient of the morphogen DPP. Among these molecules, Tolloid, a putative metalloprotease related to BMP-1, enhances DPP function, while SOG, an ortholog of the Xenopus organizer Chordin, inhibits DPP f...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80425-0
更新日期：1997-10-31 00:00:00
abstract：:Sexual reproduction predominates among eukaryotic organisms on our planet. While debate continues over why this should be so, burgeoning genomic and functional information now allows us to begin to think reasonably about some of the events that may have occurred to make sex possible in the first place. ...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/s0092-8674(01)00500-1
更新日期：2001-09-21 00:00:00
abstract：:The basal ganglia (BG) are critical for adaptive motor control, but the circuit principles underlying their pathway-specific modulation of target regions are not well understood. Here, we dissect the mechanisms underlying BG direct and indirect pathway-mediated control of the mesencephalic locomotor region (MLR), a br...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.12.037
更新日期：2016-01-28 00:00:00
abstract：:The rapid cell proliferation characteristic of early animal embryos is accomplished with an abbreviated cell cycle and no DNA replication checkpoint. Blythe and Wieschaus provide evidence that nascent zygotic transcription precedes—and may trigger—this checkpoint at the midblastula transition. ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2015.02.051
更新日期：2015-03-12 00:00:00
abstract：:PD-1 immune checkpoint blockade provides significant clinical benefits for melanoma patients. We analyzed the somatic mutanomes and transcriptomes of pretreatment melanoma biopsies to identify factors that may influence innate sensitivity or resistance to anti-PD-1 therapy. We find that overall high mutational loads a...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2016.02.065
更新日期：2016-03-24 00:00:00
abstract：:Excessive accumulation of neurofilaments in the cell bodies and proximal axons of motor neurons is a major pathological hallmark during the early stages of many human motor neuron diseases. To test directly the consequence of overexpression of the major neurofilament subunit NF-L, we produced transgenic mice that accu...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(93)90157-l
更新日期：1993-04-09 00:00:00
abstract：:Science is a powerful force for change in modern society. As the professionals at its helm, scientists have a unique responsibility to shepherd that change with thoughtful advocacy of their research and careful ethical scrutiny of their own behavior. ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2006.06.001
更新日期：2006-06-16 00:00:00
abstract：:NF-kappaB, a key regulator of the cellular inflammatory and immune response, is activated by the HTLV-I transforming and transactivating protein Tax. We show that Tax binds to the amino terminus of the protein kinase MEKK1, a component of an IkappaB kinase complex, and stimulates MEKK1 kinase activity. Tax expression ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81447-6
更新日期：1998-05-29 00:00:00
abstract：:Multipotent cardiac progenitor cells are found in the fetal and adult heart of many mammalian species including humans and form as intermediates during the differentiation of embryonic stem cells. Despite similar biological properties, the molecular identities of these different cardiac progenitor cell populations app...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2008.02.002
更新日期：2008-02-22 00:00:00
abstract：:mRNA made in eukaryotic cells typically has a 3' poly(A) tail that is added posttranscriptionally. To investigate mechanisms by which 3' poly(A) is formed, we identified the genes for the two vaccina virus-encoded polypeptides, VP55 and VP39. Primer-dependent polyadenylation activity was associated exclusively with pu...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(91)90048-4
更新日期：1991-09-20 00:00:00
abstract：:A 1.1 kb intron containing an open reading frame (ORF) in one allele (omega+) of the yeast mitochondrial 21S rRNA gene is nearly quantitatively inserted in crosses into a 21S rRNA allele lacking that intron (omega-). We have determined that this nonreciprocal exchange initiates soon after cells fuse to form zygotes an...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(85)90348-4
更新日期：1985-04-01 00:00:00
abstract：:The molecular mediator and functional significance of meal-associated brown fat (BAT) thermogenesis remains elusive. Here, we identified the gut hormone secretin as a non-sympathetic BAT activator mediating prandial thermogenesis, which consequentially induces satiation, thereby establishing a gut-secretin-BAT-brain a...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.10.016
更新日期：2018-11-29 00:00:00
abstract：:RNA transcripts are subject to posttranscriptional gene regulation involving hundreds of RNA-binding proteins (RBPs) and microRNA-containing ribonucleoprotein complexes (miRNPs) expressed in a cell-type dependent fashion. We developed a cell-based crosslinking approach to determine at high resolution and transcriptome...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2010.03.009
更新日期：2010-04-02 00:00:00
abstract：:Inflammation is associated with many neurodegenerative diseases, including Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, and multiple sclerosis. In this Review, we discuss inducers, sensors, transducers, and effectors of neuroinflammation that contribute to neuronal dysfunction and death. Al...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2010.02.016
更新日期：2010-03-19 00:00:00
abstract：:Human hepatitis B virus core protein expressed in E. coli assembles into two sizes of particle. We have determined their three-dimensional structures by electron cryomicroscopy and image processing. The large and small particles correspond to triangulation number T = 4 and T = 3 dimer clustered packings, containing 24...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(94)90142-2
更新日期：1994-06-17 00:00:00
abstract：:Nucleosomes positioned over promoters are usually inhibitory to protein binding and activity. We analyzed at the nucleotide level of resolution the nucleosomal organization of a distal, liver-specific enhancer in various mouse tissues and found that the enhancer exists in an array of three precisely positioned nucleos...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(93)80079-t
更新日期：1993-10-22 00:00:00
abstract：:Proteolysis by the ubiquitin-proteasome pathway is often regulated, but the mechanisms underlying such regulation remain ill-defined. In Saccharomyces cerevisiae, cell type is controlled by the MAT transcription factors. The alpha2 repressor is a known ubiquitin pathway substrate in alpha haploid cells. We show that a...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81421-x
更新日期：1998-07-24 00:00:00
abstract：:The transcriptional induction of heat shock genes is mediated by the heat shock transcription factor (HSF). We have investigated the oligomerization state of HSF from S. cerevisiae and find that it forms a trimer in solution and when bound to DNA. Trimerization is mediated by a region of HSF that, like the leucine zip...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(89)90604-1
更新日期：1989-12-01 00:00:00
abstract：:We describe a monoclonal antibody (anti-FC-1) that inhibits the attachment of chicken embryo fibroblasts to artificial substrates. In the immunofluorescence, anti-FC-1 also stained the focal contacts of the fibroblasts, organelles suggested to be involved in cell-substrate adhesion. This antibody was absorbed on the s...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(82)90322-1
更新日期：1982-12-01 00:00:00
abstract：:Upon heat shock, Drosophila Kc cells still contain normal cellular messenger RNAs in the cytoplasm. The distribution of these 25 degrees C mRNAs between polysomes and the postpolysomal fraction of heat-shocked cells appears unaltered as compared with control cells. The translatability of these normal cellular messages...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90155-0
更新日期：1981-02-01 00:00:00
abstract：:We describe here a bizarre organization of the single large subunit (LSU) and small subunit (SSU) rRNA genes in the mitochondrial DNA of Chlamydomonas reinhardtii. Each gene is discontinuous, with gene pieces encoding specific rRNA domains ("modules") interspersed with one another and with intact protein coding and tR...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(88)90026-8
更新日期：1988-11-04 00:00:00
abstract：:Xenogeneic spinal cord chimeras were constructed by grafting fragments of quail neural primordium into chick embryos at 2 days of incubation. Hatched birds displayed normal motor behavior for about 5 to 7 weeks, whereupon they developed a neurological syndrome; in the grafted spinal cord the pathological signs of the ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(86)90395-8
更新日期：1986-04-25 00:00:00
abstract：:Epidermal growth factor (EGF) regulates cell proliferation and differentiation by binding to the EGF receptor (EGFR) extracellular region, comprising domains I-IV, with the resultant dimerization of the receptor tyrosine kinase. In this study, the crystal structure of a 2:2 complex of human EGF and the EGFR extracellu...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(02)00963-7
更新日期：2002-09-20 00:00:00
abstract：:An important signaling pathway to the actin cytoskeleton links the Rho family GTPase Cdc42 to the actin-nucleating Arp2/3 complex through N-WASP. Nevertheless, these previously identified components are not sufficient to mediate Cdc42-induced actin polymerization in a physiological context. In this paper, we describe ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2004.06.027
更新日期：2004-07-23 00:00:00
abstract：:In this issue, Wilson et al. (2008) demonstrate that there are two functional subsets of hematopoietic stem cells that have distinctive kinetics of cell cycling. They present evidence that cells may transition between the two kinetic states, establishing one subpopulation that is ready to proliferate and another that ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2008.11.034
更新日期：2008-12-12 00:00:00
