HTLV-I Tax protein binds to MEKK1 to stimulate IkappaB kinase activity and NF-kappaB activation.


:NF-kappaB, a key regulator of the cellular inflammatory and immune response, is activated by the HTLV-I transforming and transactivating protein Tax. We show that Tax binds to the amino terminus of the protein kinase MEKK1, a component of an IkappaB kinase complex, and stimulates MEKK1 kinase activity. Tax expression increases the activity of IkappaB kinase beta (IKKbeta) to enhance phosphorylation of serine residues in IkappaB alpha that lead to its degradation. Dominant negative mutants of both IKKbeta and MEKK1 prevent Tax activation of the NF-kappaB pathway. Furthermore, recombinant MEKK1 stimulates IKKbeta phosphorylation of IkappaB alpha. Thus, Tax-mediated increases in NF-kappaB nuclear translocation result from direct interactions of Tax and MEKK1 leading to enhanced IKKbeta phosphorylation of IkappaB alpha.






Yin MJ,Christerson LB,Yamamoto Y,Kwak YT,Xu S,Mercurio F,Barbosa M,Cobb MH,Gaynor RB




Has Abstract


1998-05-29 00:00:00
















  • Mutations in the gene for EF-G reduce the requirement for 4.5S RNA in the growth of E. coli.

    abstract::A general strategy is described for the isolation of suppressors of essential genes whose functions are unknown. This strategy was used to analyze the role of 4.5S RNA, an essential RNA of E. coli. In this strategy, the structural gene for 4.5S RNA is fused to the Ptac promoter in such a way that the strain becomes de...


    pub_type: 杂志文章


    authors: Brown S

    更新日期:1987-06-19 00:00:00

  • An insertion within a variably spliced Drosophila tropomyosin gene blocks accumulation of only one encoded isoform.

    abstract::We have characterized an aberrant allele of a variably spliced Drosophila tropomyosin gene. The allele was recovered from the flightless Ifm(3)3 strain, which has been shown to have structurally and functionally abnormal indirect flight muscles. The transcribed portion of the mutant gene is interrupted by an 8,8 kb in...


    pub_type: 杂志文章


    authors: Karlik CC,Fyrberg EA

    更新日期:1985-05-01 00:00:00

  • A single gene from yeast for both nuclear and cytoplasmic polyadenylate-binding proteins: domain structure and expression.

    abstract::Nuclear and cytoplasmic poly(A)-binding proteins have been purified from Saccharomyces cerevisiae, and antisera have been used to isolate a gene that encodes them. The gene occurs in a single copy on chromosome 5 and gives rise to a unique, unspliced 2.1 kb transcript. The nuclear protein appears to be derived from th...


    pub_type: 杂志文章


    authors: Sachs AB,Bond MW,Kornberg RD

    更新日期:1986-06-20 00:00:00

  • Will the ubiquitin system furnish as many drug targets as protein kinases?

    abstract::Protein phosphorylation and protein ubiquitination regulate most aspects of cell life, and defects in these control mechanisms cause cancer and many other diseases. In the past decade, protein kinases have become one of the most important classes of drug targets for the pharmaceutical industry. In contrast, drug disco...


    pub_type: 杂志文章


    authors: Cohen P,Tcherpakov M

    更新日期:2010-11-24 00:00:00

  • A major step on the road to understanding a unique posttranslational modification and its role in a genetic disease.

    abstract::The posttranslational conversion of cysteine to C(alpha)-formylglycine in the catalytic site of mammalian sulfatases is deficient in the rare but devastating disorder multiple sulfatase deficiency (MSD). Two papers in this issue of Cell report the cloning of a gene responsible for this activity. ...


    pub_type: 评论,杂志文章,评审


    authors: Baenziger JU

    更新日期:2003-05-16 00:00:00

  • Targeted disruption of the trkB neurotrophin receptor gene results in nervous system lesions and neonatal death.

    abstract::We have generated mice carrying a germline mutation in the tyrosine kinase catalytic domain of the trkB gene. This mutation eliminates expression of gp145trkB, a protein-tyrosine kinase that serves as the signaling receptor for two members of the nerve growth factor family of neurotrophins, brain-derived neurotrophic ...


    pub_type: 杂志文章


    authors: Klein R,Smeyne RJ,Wurst W,Long LK,Auerbach BA,Joyner AL,Barbacid M

    更新日期:1993-10-08 00:00:00

  • Functional and genomic analyses reveal an essential coordination between the unfolded protein response and ER-associated degradation.

    abstract::The unfolded protein response (UPR) regulates gene expression in response to stress in the endoplasmic reticulum (ER). We determined the transcriptional scope of the UPR using DNA microarrays. Rather than regulating only ER-resident chaperones and phospholipid biosynthesis, as anticipated from earlier work, the UPR af...


    pub_type: 杂志文章


    authors: Travers KJ,Patil CK,Wodicka L,Lockhart DJ,Weissman JS,Walter P

    更新日期:2000-04-28 00:00:00

  • Changes in gene position are accompanied by a change in time of replication.

    abstract::The globin and immunoglobulin multigene families have been used to study the effect of chromosomal organization on the time of gene replication. Some of the genes are late-replicating, providing the first identification of late-replicating sequences that are not highly repetitive. One is a member of the mouse alpha-gl...


    pub_type: 杂志文章


    authors: Calza RE,Eckhardt LA,DelGiudice T,Schildkraut CL

    更新日期:1984-03-01 00:00:00

  • RNA polymerase interactions with the upstream region of the E. coli tyrT promoter.

    abstract::The rate of in vivo transcription from the E. coli tRNA and rRNA promoters depends on both cellular growth rate and aminoacid availability. To investigate the molecular mechanisms involved we determined the extent of interaction of RNA polymerase with the promoter of the tyrT stable RNA gene. We show that the enzyme c...


    pub_type: 杂志文章


    authors: Travers AA,Lamond AI,Mace HA,Berman ML

    更新日期:1983-11-01 00:00:00

  • Beta-arrestin1 regulates zebrafish hematopoiesis through binding to YY1 and relieving polycomb group repression.

    abstract::Beta-arrestin1 is a multifunctional protein critically involved in signal transduction. Recently, it is also identified as a nuclear transcriptional regulator, but the underlying mechanisms and physiological significance remain to be explored. Here, we identified beta-arrestin1 as an evolutionarily conserved protein e...


    pub_type: 杂志文章


    authors: Yue R,Kang J,Zhao C,Hu W,Tang Y,Liu X,Pei G

    更新日期:2009-10-30 00:00:00

  • Renal Sodium Gradient Orchestrates a Dynamic Antibacterial Defense Zone.

    abstract::Lower urinary tract infections are among the most common human bacterial infections, but extension to the kidneys is rare. This has been attributed to mechanical forces, such as urine flow, that prevent the ascent of bladder microbes. Here, we show that the regional hypersalinity, required for the kidney's urine-conce...


    pub_type: 杂志文章


    authors: Berry MR,Mathews RJ,Ferdinand JR,Jing C,Loudon KW,Wlodek E,Dennison TW,Kuper C,Neuhofer W,Clatworthy MR

    更新日期:2017-08-24 00:00:00

  • Formation of a keratinizing epithelium in culture by a cloned cell line derived from a teratoma.

    abstract::From a transplantable mouse teratoma it has been possible to derive an established keratinizing cell line (XB) which grows well in cultures containing lethally irradiated 3T3 fibroblasts at the correct density. Single cells of the keratinizing line grow into colonies each consisting of a stratified squamous epithelium...


    pub_type: 杂志文章


    authors: Rheinwald JG,Green H

    更新日期:1975-11-01 00:00:00

  • Phase transition of spindle-associated protein regulate spindle apparatus assembly.

    abstract::Spindle assembly required during mitosis depends on microtubule polymerization. We demonstrate that the evolutionarily conserved low-complexity protein, BuGZ, undergoes phase transition or coacervation to promote assembly of both spindles and their associated components. BuGZ forms temperature-dependent liquid droplet...


    pub_type: 杂志文章


    authors: Jiang H,Wang S,Huang Y,He X,Cui H,Zhu X,Zheng Y

    更新日期:2015-09-24 00:00:00

  • Splicing misplaced.

    abstract::Newly synthesized transcripts are usually spliced during transcription or immediately thereafter. So pre-mRNA splicing has been presumed to occur exclusively in the cell nucleus. In this issue of Cell, Denis et al. (2005) now report the presence of functional spliceosomes and signal-dependent pre-mRNA splicing in the ...


    pub_type: 评论,杂志文章,评审


    authors: Meshorer E,Misteli T

    更新日期:2005-08-12 00:00:00

  • The BELL1 gene encodes a homeodomain protein involved in pattern formation in the Arabidopsis ovule primordium.

    abstract::Ovule development in Arabidopsis involves the formation of three morphologically defined proximal-distal pattern elements. Integuments arise from the central pattern element. Analysis of Bell 1 (Bel 1) mutant ovules indicated that BEL1 was required for integument development. Cloning of the BEL1 locus reveals that it ...


    pub_type: 杂志文章


    authors: Reiser L,Modrusan Z,Margossian L,Samach A,Ohad N,Haughn GW,Fischer RL

    更新日期:1995-12-01 00:00:00

  • Stereo immunofluorescence microscopy: I. Three-dimensional arrangement of microfilaments, microtubules and tonofilaments.

    abstract::An easy manipulation of the commercial fluorescence microscope allows stereo pairs of pictures to be taken, which when examined with a stereo viewer, give a strong three-dimensional impression. The procedure is described in detail. Its use allows the documentation by immunofluorescence microscopy of the three-dimensio...


    pub_type: 杂志文章


    authors: Osborn M,Born T,Koitsch HJ,Weber K

    更新日期:1978-07-01 00:00:00

  • Free intermingling of mammalian beta-tubulin isotypes among functionally distinct microtubules.

    abstract::Mammalian cells express a spectrum of tubulin isotypes whose relationship to the diversity of microtubule function is unknown. To examine whether different isotypes are segregated into functionally distinct microtubules, we generated immune sera capable of discriminating among the various naturally occurring beta-tubu...


    pub_type: 杂志文章


    authors: Lewis SA,Gu W,Cowan NJ

    更新日期:1987-05-22 00:00:00

  • Isolation of two genes that affect mitotic chromosome transmission in S. cerevisiae.

    abstract::Two DNA sequences that reduce mitotic fidelity of chromosome transmission have been identified: MIF1 and MIF2. MIF1 is a unique sequence located on the right arm of chromosome XII that stimulates loss and recombination for both chromosomes V and VII when present in a high copy number plasmid. MIF1 is not essential for...


    pub_type: 杂志文章


    authors: Meeks-Wagner D,Wood JS,Garvik B,Hartwell LH

    更新日期:1986-01-17 00:00:00

  • Architecture of the 90S Pre-ribosome: A Structural View on the Birth of the Eukaryotic Ribosome.

    abstract::The 90S pre-ribosome is an early biogenesis intermediate formed during co-transcriptional ribosome formation, composed of ∼70 assembly factors and several small nucleolar RNAs (snoRNAs) that associate with nascent pre-rRNA. We report the cryo-EM structure of the Chaetomium thermophilum 90S pre-ribosome, revealing how ...


    pub_type: 杂志文章


    authors: Kornprobst M,Turk M,Kellner N,Cheng J,Flemming D,Koš-Braun I,Koš M,Thoms M,Berninghausen O,Beckmann R,Hurt E

    更新日期:2016-07-14 00:00:00

  • Noncanonical MMS2-encoded ubiquitin-conjugating enzyme functions in assembly of novel polyubiquitin chains for DNA repair.

    abstract::Ubiquitin-conjugating enzyme variant (UEV) proteins resemble ubiquitin-conjugating enzymes (E2s) but lack the defining E2 active-site residue. The MMS2-encoded UEV protein has been genetically implicated in error-free postreplicative DNA repair in Saccharomyces cerevisiae. We show that Mms2p forms a specific heteromer...


    pub_type: 杂志文章


    authors: Hofmann RM,Pickart CM

    更新日期:1999-03-05 00:00:00

  • Prokaryotic Argonautes Function beyond Immunity by Unlinking Replicating Chromosomes.

    abstract::Eukaryotic Argonaute proteins strictly mediate RNA-guided RNA interference. In contrast, prokaryotic Argonautes can utilize DNA guides to target complementary DNA sequences to protect their hosts against invading DNA. In this issue of Cell, Jolly and colleagues demonstrate that Thermus thermophilus Argonaute additiona...


    pub_type: 杂志文章


    authors: Swarts DC

    更新日期:2020-09-17 00:00:00

  • Identification of early replicating fragile sites that contribute to genome instability.

    abstract::DNA double-strand breaks (DSBs) in B lymphocytes arise stochastically during replication or as a result of targeted DNA damage by activation-induced cytidine deaminase (AID). Here we identify recurrent, early replicating, and AID-independent DNA lesions, termed early replication fragile sites (ERFSs), by genome-wide l...


    pub_type: 杂志文章,评审


    authors: Barlow JH,Faryabi RB,Callén E,Wong N,Malhowski A,Chen HT,Gutierrez-Cruz G,Sun HW,McKinnon P,Wright G,Casellas R,Robbiani DF,Staudt L,Fernandez-Capetillo O,Nussenzweig A

    更新日期:2013-01-31 00:00:00

  • Raphe Circuits on the Menu.

    abstract::The dorsal raphe nucleus (DRN) is an important brain area for body-weight regulation. In this issue of Cell, Nectow et al. uncover cell-type-specific neural circuitry and pharmacology for appetite control within the DRN. ...


    pub_type: 评论,杂志文章


    authors: Yang H,Sternson SM

    更新日期:2017-07-27 00:00:00

  • Chk1 suppresses a caspase-2 apoptotic response to DNA damage that bypasses p53, Bcl-2, and caspase-3.

    abstract::Evasion of DNA damage-induced cell death, via mutation of the p53 tumor suppressor or overexpression of prosurvival Bcl-2 family proteins, is a key step toward malignant transformation and therapeutic resistance. We report that depletion or acute inhibition of checkpoint kinase 1 (Chk1) is sufficient to restore gamma-...


    pub_type: 杂志文章


    authors: Sidi S,Sanda T,Kennedy RD,Hagen AT,Jette CA,Hoffmans R,Pascual J,Imamura S,Kishi S,Amatruda JF,Kanki JP,Green DR,D'Andrea AA,Look AT

    更新日期:2008-05-30 00:00:00

  • Tempering temperature changes for robust development.

    abstract::Developmental signaling pathways needed to evolve to be robust against environmental fluctuations. In this issue, Shimizu et al. reveal a complex system of interacting endocytic pathways that help to maintain consistent levels of Notch activity across a range of temperatures. ...


    pub_type: 评论,杂志文章


    authors: Delidakis C

    更新日期:2014-05-22 00:00:00

  • Identification and mapping of the transcriptional and translational products of the yeast plasmid, 2mu circle.

    abstract::We have identified two major and approximately ten minor poly(A)-containing RNA species in S. cerevisiae which arise from in vivo transcription of the yeast plasmid, known as 2mu circle. The two major species, which are 1325 and 1275 bases in length, are transcribed from the two unique halves of the plasmid and extend...


    pub_type: 杂志文章


    authors: Broach JR,Atkins JF,McGill C,Chow L

    更新日期:1979-04-01 00:00:00

  • Rumi is a CAP10 domain glycosyltransferase that modifies Notch and is required for Notch signaling.

    abstract::Notch signaling is broadly used to regulate cell-fate decisions. We have identified a gene, rumi, with a temperature-sensitive Notch phenotype. At 28 degrees C-30 degrees C, rumi clones exhibit a full-blown loss of Notch signaling in all tissues tested. However, at 18 degrees C only a mild Notch phenotype is evident. ...


    pub_type: 杂志文章


    authors: Acar M,Jafar-Nejad H,Takeuchi H,Rajan A,Ibrani D,Rana NA,Pan H,Haltiwanger RS,Bellen HJ

    更新日期:2008-01-25 00:00:00

  • Multiscale Structuring of the E. coli Chromosome by Nucleoid-Associated and Condensin Proteins.

    abstract::As in eukaryotes, bacterial genomes are not randomly folded. Bacterial genetic information is generally carried on a circular chromosome with a single origin of replication from which two replication forks proceed bidirectionally toward the opposite terminus region. Here, we investigate the higher-order architecture o...


    pub_type: 杂志文章


    authors: Lioy VS,Cournac A,Marbouty M,Duigou S,Mozziconacci J,Espéli O,Boccard F,Koszul R

    更新日期:2018-02-08 00:00:00

  • A chromosome RNAissance.

    abstract::In RNA-mediated interference (RNAi), double-stranded RNAs (dsRNAs) target complementary mRNAs for degradation. New work demonstrates that essential chromosomal functions are mediated through RNAi protein components and short RNAs, which alter chromosome function at specific DNA loci via histone modification. ...


    pub_type: 杂志文章


    authors: Dernburg AF,Karpen GH

    更新日期:2002-10-18 00:00:00

  • Formation of a rate-limiting intermediate in 5S RNA gene transcription.

    abstract::We have performed in vitro kinetic analyses of transcription from the 5S gene promoter in order to resolve the rate-limiting events which lead to accurate transcription of the 5S RNA gene. We demonstrate that a rate-limiting intermediate can be formed during an extended incubation prior to initiation of transcription....


    pub_type: 杂志文章


    authors: Bieker JJ,Martin PL,Roeder RG

    更新日期:1985-01-01 00:00:00