Trimerization of a yeast transcriptional activator via a coiled-coil motif.

Abstract:

:The transcriptional induction of heat shock genes is mediated by the heat shock transcription factor (HSF). We have investigated the oligomerization state of HSF from S. cerevisiae and find that it forms a trimer in solution and when bound to DNA. Trimerization is mediated by a region of HSF that, like the leucine zipper, is characterized by the occurrence of hydrophobic amino acids every 7 residues. We propose that it forms a three-stranded coiled coil.

journal_name

Cell

journal_title

Cell

authors

Sorger PK,Nelson HC

doi

10.1016/0092-8674(89)90604-1

subject

Has Abstract

pub_date

1989-12-01 00:00:00

pages

807-13

issue

5

eissn

0092-8674

issn

1097-4172

pii

0092-8674(89)90604-1

journal_volume

59

pub_type

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