Abstract：
:Membrane scission is essential for intracellular trafficking. While BAR domain proteins such as endophilin have been reported in dynamin-independent scission of tubular membrane necks, the cutting mechanism has yet to be deciphered. Here, we combine a theoretical model, in vitro, and in vivo experiments revealing how protein scaffolds may cut tubular membranes. We demonstrate that the protein scaffold bound to the underlying tube creates a frictional barrier for lipid diffusion; tube elongation thus builds local membrane tension until the membrane undergoes scission through lysis. We call this mechanism friction-driven scission (FDS). In cells, motors pull tubes, particularly during endocytosis. Through reconstitution, we show that motors not only can pull out and extend protein-scaffolded tubes but also can cut them by FDS. FDS is generic, operating even in the absence of amphipathic helices in the BAR domain, and could in principle apply to any high-friction protein and membrane assembly.
journal_name
Celljournal_title
Cellauthors
Simunovic M,Manneville JB,Renard HF,Evergren E,Raghunathan K,Bhatia D,Kenworthy AK,Voth GA,Prost J,McMahon HT,Johannes L,Bassereau P,Callan-Jones Adoi
10.1016/j.cell.2017.05.047subject
Has Abstractpub_date
2017-06-29 00:00:00pages
172-184.e11issue
1eissn
0092-8674issn
1097-4172pii
S0092-8674(17)30640-2journal_volume
170pub_type
杂志文章相关文献
CELL文献大全abstract：:Science is a powerful force for change in modern society. As the professionals at its helm, scientists have a unique responsibility to shepherd that change with thoughtful advocacy of their research and careful ethical scrutiny of their own behavior. ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2006.06.001
更新日期：2006-06-16 00:00:00
abstract：:The maternal Dorsal regulatory gradient initiates the differentiation of several tissues in the early Drosophila embryo. Whole-genome microarray assays identified as many as 40 new Dorsal target genes, which encode a broad spectrum of cell signaling proteins and transcription factors. Evidence is presented that a tiss...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(02)01087-5
更新日期：2002-11-27 00:00:00
abstract：:Eukaryotic cells have developed mechanisms for regulating the nuclear transport of macromolecules that control various cellular events including movement through defined stages of the cell cycle. In yeast cells, where the nuclear envelope remains intact throughout the cell cycle, these transport regulatory mechanisms ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(03)00986-3
更新日期：2003-12-26 00:00:00
abstract：:Muscle cell cultures from Drosophila melanogaster were obtained by plating dissociated gastrula stage embryo cells on protamine-treated culture dishes. They myogenic cells in these cultures fuse to form multinucleated pulsating cells by 15 hr after plating. An analysis of protein synthesis during myogenesis in these c...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(78)90210-6
更新日期：1978-04-01 00:00:00
abstract：:The transcription factor NRF2 is a master regulator of the cellular antioxidant response, and it is often genetically activated in non-small-cell lung cancers (NSCLCs) by, for instance, mutations in the negative regulator KEAP1. While direct pharmacological inhibition of NRF2 has proven challenging, its aberrant activ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.08.051
更新日期：2017-10-19 00:00:00
abstract：:The precursor of the 55K adenovirus terminal protein is an 87K protein that is covalently linked to viral DNA. This protein is likely to be identical to the 80,000 dalton protein described by Challberg et al. (1980). The mRNA for the 87K terminal protein precursor, like that for the E2-72K DNA binding protein, is dete...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90145-8
更新日期：1981-02-01 00:00:00
abstract：:TOR complex 1 (TORC1) is a potent anabolic regulator of cellular growth and metabolism. When cells have sufficient amino acids, TORC1 is active due to its lysosomal localization mediated via the Rag GTPases. Upon amino acid removal, the Rag GTPases release TORC1, causing it to become cytoplasmic and inactive. We show ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.01.024
更新日期：2014-02-13 00:00:00
abstract：:The detection and discrimination of odorants in mammals is thought to be mediated by a family of 100-1000 seven transmembrane domain receptor proteins, although none of these putative olfactory receptors have been shown to bind individual odorants with high affinity. We have used a genetic approach to identify the gen...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(95)90118-3
更新日期：1995-11-03 00:00:00
abstract：:Telomerase is the ribonucleoprotein enzyme that replenishes telomeric DNA and maintains genome integrity. Minimally, telomerase activity requires a templating RNA and a catalytic protein. Additional proteins are required for activity on telomeres in vivo. Here, we report that the Pop1, Pop6, and Pop7 proteins, known c...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2016.04.018
更新日期：2016-05-19 00:00:00
abstract：:Cellular functioning requires the orchestration of thousands of molecular interactions in time and space. Yet most molecules in a cell move by diffusion, which is sensitive to external factors like temperature. How cells sustain complex, diffusion-based systems across wide temperature ranges is unknown. Here, we uncov...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2020.10.017
更新日期：2020-12-10 00:00:00
abstract：:DMC1 is a new meiosis-specific yeast gene. Dmc1 protein is structurally similar to bacterial RecA proteins. dmc1 mutants are defective in reciprocal recombination, accumulate double-strand break (DSB) recombination intermediates, fail to form normal synaptonemal complex (SC), and arrest late in meiotic prophase. dmc1 ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(92)90446-j
更新日期：1992-05-01 00:00:00
abstract：:A diverse range of ultimate chemical carcinogens inhibited the transfer of methyl groups from S-adenosylmethionine to hemimethylated DNA in a reaction catalyzed by mouse spleen methyltransferase. The formation of alkali-labile sites in DNA lessened its ability to accept methyl groups in vitro, but the methylation reac...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90514-7
更新日期：1983-01-01 00:00:00
abstract：:The histone tails on the nucleosome surface are subject to enzyme-catalyzed modifications that may, singly or in combination, form a code specifying patterns of gene expression. Recent papers provide insights into how a combinatorial code might be set and read. They show how modification of one residue can influence t...
journal_title：Cell
pub_type： 评论,杂志文章,评审
doi：10.1016/s0092-8674(02)01080-2
更新日期：2002-11-01 00:00:00
abstract：:Much of the Y chromosome consists of large palindromic arrays harboring genes that are critical for spermatogenesis. In this issue, Lange et al. (2009) show that although gene conversion within these arrays maintains their integrity, it also permits rare unequal sister chromatid-exchange events within palindromes that...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2009.08.022
更新日期：2009-09-04 00:00:00
abstract：:Propagation of a species requires periodic cell renewal to avoid clonal senescence. Liu et al. (2010) now describe a new mechanism of cell renewal in budding yeast, in which damaged protein aggregates are transported out of the daughter buds along actin cables to preserve youthfulness. ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2010.01.015
更新日期：2010-01-22 00:00:00
abstract：:Double-strand break (DSB) repair is critical for maintaining genomic integrity and requires the processing of the 5' DSB ends. Recent studies have shed light on the mechanism and regulation of DNA end processing during DSB repair by homologous recombination. ...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2009.05.007
更新日期：2009-05-29 00:00:00
abstract：:A key aspect of genomic medicine is to make individualized clinical decisions from personal genomes. We developed a machine-learning framework to integrate personal genomes and electronic health record (EHR) data and used this framework to study abdominal aortic aneurysm (AAA), a prevalent irreversible cardiovascular ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.07.021
更新日期：2018-09-06 00:00:00
abstract：:S. cerevisiae inherits its vacuole by projecting vacuole-derived membrane vesicles and tubules into the bud, where they fuse to establish the daughter vacuole. This homotypic fusion event can be assayed in vitro. It requires Sec17p and Sec18p, the homologs of the mammalian alpha-SNAP and NSF, which cooperate in multip...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81084-3
更新日期：1996-04-05 00:00:00
abstract：:Females but not males of the low-lymphoma RF/J strain transmit a non-Mendelian factor which suppresses the development of lymphoma in F1 crosses with mice of the high-lymphoma AKR/J strain. Suppression of lymphoma was also evident in the first backcross generation to the parental AKR strain, but only when (RF female x...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(80)90517-6
更新日期：1980-02-01 00:00:00
abstract：:The exchange of metabolites between the mitochondrial matrix and the cytosol depends on β-barrel channels in the outer membrane and α-helical carrier proteins in the inner membrane. The essential translocase of the inner membrane (TIM) chaperones escort these proteins through the intermembrane space, but the structura...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.10.039
更新日期：2018-11-15 00:00:00
abstract：:Signaling proteins from the same family can have markedly different roles in a given cellular context. Here, we show that expression of one hundred constitutively active human small GTPases induced cell morphologies that fell into nine distinct classes. We developed an algorithm for pairs of classes that predicted ami...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(03)00315-5
更新日期：2003-05-02 00:00:00
abstract：:Based on the above mechanisms of mRNA degradation, an integrated model of mRNA turnover can be proposed (Figure 1). In this model, all polyadenylated mRNAs would be degraded by the deadenylation-dependent pathway at some rate. In addition to this default pathway, another layer of complexity would come from degradation...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/0092-8674(95)90326-7
更新日期：1995-04-21 00:00:00
abstract：:Clinical trials are key to translating scientific advances into progress in cancer research and care. Confronted by developments in basic science, the landscape of clinical cancer research is rapidly evolving. Here, we review recent changes in clinical trials' design and conduct, and we forecast future directions towa...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.01.027
更新日期：2017-02-09 00:00:00
abstract：:Ions serve as essential nutrients in higher plants and can also act as signaling molecules. Little is known about how plants sense changes in soil nutrient concentrations. Previous studies showed that T101-phosphorylated CHL1 is a high-affinity nitrate transporter, whereas T101-dephosphorylated CHL1 is a low-affinity ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2009.07.004
更新日期：2009-09-18 00:00:00
abstract：:SecA, the peripheral subunit of E. coli preprotein translocase, alternates between a membrane inserted and a deinserted state as part of the catalytic cycle of preprotein translocation. When SecA is complexed with SecY/E and preprotein, ATP drives a profound conformational change, leading to membrane insertion of a 30...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(94)90582-7
更新日期：1994-09-09 00:00:00
abstract：:Initiator tRNAs from the cytoplasm of salmon testes and salmon liver have been purified. The nucleotide sequence of these initiator tRNAs has been determined and found identical to that of initiator tRNA from mammalian cytoplasm. The only difference is the extent of modification of the nuceloside located between the d...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(75)90189-0
更新日期：1975-11-01 00:00:00
abstract：:Yeast chromosomes terminate in tracts of simple repetitive DNA (poly[G1-3T]). Mutations in the gene TEL1 result in shortened telomeres. Sequence analysis of TEL1 indicates that it encodes a very large (322 kDa) protein with amino acid motifs found in phosphatidylinositol/protein kinases. The closest homolog to TEL1 is...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(95)90479-4
更新日期：1995-09-08 00:00:00
abstract：:Notch signaling is a key developmental pathway that is subject to frequent genetic and epigenetic perturbations in many different human tumors. Here we investigate whether long noncoding RNA (lncRNA) genes, in addition to mRNAs, are key downstream targets of oncogenic Notch1 in human T cell acute lymphoblastic leukemi...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.05.049
更新日期：2014-07-31 00:00:00
abstract：:Telomerase activity was demonstrated in cell-free extracts from S. cerevisiae through the use of a PCR-based assay. As expected, this activity was eliminated by RNase or phenol treatment of the extract and was dependent on dGTP and dTTP. Telomerase was not detected in extracts prepared from cells grown for approximate...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(05)80017-0
更新日期：1995-06-30 00:00:00
abstract：:H2S is an endogenous gasotransmitter that plays an important role in physiological conditions. In this issue, Das et al. provide evidence that SIRT1-dependent angiogenesis is augmented by H2S-findings reinforced by Longchamp et al., who demonstrate that H2S-dependent angiogenesis is triggered by amino acid deprivation...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2018.03.011
更新日期：2018-03-22 00:00:00
