Switch-of-function mutants based on morphology classification of Ras superfamily small GTPases.

abstract：:The subcellular distribution of the type II enzyme of cAMP-dependent protein kinase (cAMP-dPK II) in epithelial and fibroblastic cells was determined by indirect immunofluorescence microscopy. In interphase cells both regulatory (R II) and catalytic (C) subunits were concentrated in a perinuclear area. By comparison o...

journal_title：Cell

authors： Nigg EA,Schäfer G,Hilz H,Eppenberger HM

更新日期：1985-07-01 00:00:00

abstract：:ATP-dependent proteases are vital to maintain cellular protein homeostasis. Here, we study the mechanisms of force generation and intersubunit coordination in the ClpXP protease from E. coli to understand how these machines couple ATP hydrolysis to mechanical protein unfolding. Single-molecule analyses reveal that pho...

journal_title：Cell

authors： Sen M,Maillard RA,Nyquist K,Rodriguez-Aliaga P,Pressé S,Martin A,Bustamante C

更新日期：2013-10-24 00:00:00

abstract：:We have cloned and characterized the rat telomerase protein component 1 gene (TLP1), which is related to the gene for Tetrahymena p80. The cDNA encodes a 2629 amino acid sequence and produces the TLP1 proteins p240 and p230. The anti-TLP1 antibody specifically immunoprecipitated the telomerase activity. Moreover, p240...

journal_title：Cell

authors： Nakayama J,Saito M,Nakamura H,Matsuura A,Ishikawa F

更新日期：1997-03-21 00:00:00

abstract：:Transfer of human chromosome 11, which contains the myoD locus, from primary fibroblasts into 10T1/2 cells results in activation of myoD. In contrast, hybrids that retain human chromosome 11 and additional human chromosomes fail to activate myoD. We show that human chromosome 4 inhibits myoD activation. myoD enhancer/...

journal_title：Cell

authors： Woloshin P,Song K,Degnin C,Killary AM,Goldhamer DJ,Sassoon D,Thayer MJ

更新日期：1995-08-25 00:00:00

abstract：:The bHLH-ZIP protein Mad heterodimerizes with Max as a sequence-specific transcriptional repressor. Mad is rapidly induced upon differentiation, and the associated switch from Myc-Max to Mad-Max heterocomplexes seem to repress genes normally activated by Myc-Max. We have identified two related mammalian cDNAs that enc...

journal_title：Cell

authors： Ayer DE,Lawrence QA,Eisenman RN

更新日期：1995-03-10 00:00:00

abstract：:tDNA sequences essential for promotion of transcription have been identified for a tRNA1met gene of X. laevis. A cloned tRNA gene unit was altered by resection of the 5' flanking sequences or by specific deletions of gene and trailing sequences. Gene internal sequences were also substituted by unrelated sequences. The...

journal_title：Cell

authors： Hofstetter H,Kressman A,Birnstiel ML

更新日期：1981-05-01 00:00:00

abstract：:In this issue of Cell, a study by Valerie Mizrahi and her colleagues suggests that a putative error-prone DNA polymerase encoded by the dnaE2 gene of Mycobacterium tuberculosis may bypass certain types of DNA base damage, generating mutations. This may be an important mechanism for generating drug-resistant strains of...

journal_title：Cell

authors： Friedberg EC,Fischhaber PL

更新日期：2003-04-18 00:00:00

abstract：:The steroid hormone ecdysone triggers coordinate changes in Drosophila tissue development that result in metamorphosis. To advance our understanding of the genetic regulatory hierarchies controlling this tissue response, we have isolated and characterized a gene, EcR, for a new steroid receptor homolog and have shown ...

journal_title：Cell

authors： Koelle MR,Talbot WS,Segraves WA,Bender MT,Cherbas P,Hogness DS

更新日期：1991-10-04 00:00:00

abstract：:The accurate timing and execution of organelle biogenesis is crucial for cell physiology. Centriole biogenesis is regulated by Polo-like kinase 4 (Plk4) and initiates in S-phase when a daughter centriole grows from the side of a pre-existing mother. Here, we show that a Plk4 oscillation at the base of the growing cent...

journal_title：Cell

authors： Aydogan MG,Steinacker TL,Mofatteh M,Wilmott ZM,Zhou FY,Gartenmann L,Wainman A,Saurya S,Novak ZA,Wong SS,Goriely A,Boemo MA,Raff JW

更新日期：2020-06-25 00:00:00

abstract：:Synaptotagmin is an abundant synaptic vesicle-associated protein proposed to be involved in calcium-mediated neurotransmitter release. Our molecular and genetic results demonstrate that, although synaptotagmin is required for the proper function of the presynaptic nerve terminal in C. elegans, some neurotransmitter re...

journal_title：Cell

authors： Nonet ML,Grundahl K,Meyer BJ,Rand JB

更新日期：1993-07-02 00:00:00

abstract：:Enduring forms of synaptic plasticity and memory require new protein synthesis, but little is known about the underlying regulatory mechanisms. Here, we investigate the role of MAPK signaling in these processes. Conditional expression of a dominant-negative form of MEK1 in the postnatal murine forebrain inhibited ERK ...

journal_title：Cell

authors： Kelleher RJ 3rd,Govindarajan A,Jung HY,Kang H,Tonegawa S

更新日期：2004-02-06 00:00:00

abstract：:Pathogenic simian immunodeficiency virus (SIV) infection is associated with enteropathy, which likely contributes to AIDS progression. To identify candidate etiologies for AIDS enteropathy, we used next-generation sequencing to define the enteric virome during SIV infection in nonhuman primates. Pathogenic, but not no...

journal_title：Cell

authors： Handley SA,Thackray LB,Zhao G,Presti R,Miller AD,Droit L,Abbink P,Maxfield LF,Kambal A,Duan E,Stanley K,Kramer J,Macri SC,Permar SR,Schmitz JE,Mansfield K,Brenchley JM,Veazey RS,Stappenbeck TS,Wang D,Barouch DH,

更新日期：2012-10-12 00:00:00

abstract：:Newly synthesized transcripts are usually spliced during transcription or immediately thereafter. So pre-mRNA splicing has been presumed to occur exclusively in the cell nucleus. In this issue of Cell, Denis et al. (2005) now report the presence of functional spliceosomes and signal-dependent pre-mRNA splicing in the ...

journal_title：Cell

authors： Meshorer E,Misteli T

更新日期：2005-08-12 00:00:00

abstract：:Identifying the molecular mechanisms that underlie aging and their pharmacological manipulation are key aims for improving lifelong human health. Here, we identify a critical role for Ras-Erk-ETS signaling in aging in Drosophila. We show that inhibition of Ras is sufficient for lifespan extension downstream of reduced...

journal_title：Cell

authors： Slack C,Alic N,Foley A,Cabecinha M,Hoddinott MP,Partridge L

更新日期：2015-07-02 00:00:00

abstract：:Chronic Pseudomonas aeruginosa infections evade antibiotic therapy and are associated with mortality in cystic fibrosis (CF) patients. We find that in vitro resistance evolution of P. aeruginosa toward clinically relevant antibiotics leads to phenotypic convergence toward distinct states. These states are associated w...

journal_title：Cell

authors： Imamovic L,Ellabaan MMH,Dantas Machado AM,Citterio L,Wulff T,Molin S,Krogh Johansen H,Sommer MOA

更新日期：2018-01-11 00:00:00

abstract：:Dentatorubral-pallidoluysian atrophy is a progressive neurodegenerative disease caused by the expansion of a polyglutamine repeats within the Atrophin-1 protein. The in vivo function of Atrophin-1 is unknown. We have characterized a Drosophila gene encoding an Atrophin family protein. Analysis of mutant phenotypes ind...

journal_title：Cell

authors： Zhang S,Xu L,Lee J,Xu T

更新日期：2002-01-11 00:00:00

abstract：:Transcriptional analysis of SV40 late promoter mutants indicates that the DNA sequence 5'-GGTACCTAACC-3' (map positions 294-304) is important in the control of SV40 late RNA expression. C to T base substitutions at map positions 298, 299 and 304 increased initiation of late RNA synthesis at map position 325 five to te...

journal_title：Cell

authors： Brady J,Radonovich M,Vodkin M,Natarajan V,Thoren M,Das G,Janik J,Salzman NP

更新日期：1982-12-01 00:00:00

abstract：:Polyadenylation of mRNA precursors by poly(A) polymerase depends on a specificity factor, CPF, recognizing the polyadenylation signal AAUAAA. This paper describes an apparently novel poly(A)-binding protein that acts as a second specificity factor, mediating the recognition of the growing poly(A) tail. A transition fr...

journal_title：Cell

authors： Wahle E

更新日期：1991-08-23 00:00:00

abstract：:X-ray studies have established that the structure of a right-handed, Watson-Crick double helix can change from place to place along its length as a function of base sequence. The base pairs transmit deformations out to the phosphate backbone, where they can then be recognized by proteins and other DNA-binding reagents...

journal_title：Cell

authors： Drew HR,Travers AA

更新日期：1984-06-01 00:00:00

abstract：:During aging, stromal functions are thought to be impaired, but little is known whether this stems from changes of fibroblasts. Using population- and single-cell transcriptomics, as well as long-term lineage tracing, we studied whether murine dermal fibroblasts are altered during physiological aging under different di...

journal_title：Cell

authors： Salzer MC,Lafzi A,Berenguer-Llergo A,Youssif C,Castellanos A,Solanas G,Peixoto FO,Stephan-Otto Attolini C,Prats N,Aguilera M,Martín-Caballero J,Heyn H,Benitah SA

更新日期：2018-11-29 00:00:00

abstract：:A gene, bcl-3, is found on chromosome 19 adjacent to the breakpoints in the translocation t(14;19)(q32;q13.1), which occurs in some cases of chronic lymphocytic leukemia. Sequence analysis of the human bcl-3 gene predicts a protein containing seven tandem copies of the SWI6/cdc10 motif. This motif was previously ident...

journal_title：Cell

authors： Ohno H,Takimoto G,McKeithan TW

更新日期：1990-03-23 00:00:00

abstract：:Linear simian virus 40 (SV40) chromosomes were prepared by Eco R1 nuclease cleavage of the circular SV40 chromosomes released from virions with dithiothreitol at pH 9,8. Chromatin-DNA hybrids were constructed with segments of 3H-labeled, naked SV40 DNA covalently joined via the Eco R1-generated cohesive ends to segmen...

journal_title：Cell

authors： Beard P

更新日期：1978-11-01 00:00:00

abstract：:Gastrointestinal enterochromaffin cells regulate bone and gut homeostasis via serotonin (5-hydroxytryptamine [5-HT]) production. A recent report suggested that gut microbes regulate 5-HT levels; however, the precise underlying molecular mechanisms are unexplored. Here, we reveal that the cation channel Piezo1 in the g...

journal_title：Cell

authors： Sugisawa E,Takayama Y,Takemura N,Kondo T,Hatakeyama S,Kumagai Y,Sunagawa M,Tominaga M,Maruyama K

更新日期：2020-08-06 00:00:00

abstract：:In this issue of Cell, Coppens and coworkers (Coppens et al., 2006) describe how Toxoplasma gondii, an obligate intracellular parasite, feeds on the host. Coppens et al. provide evidence that the parasite takes host cell endosomes and lysosomes hostage by sequestering them where the parasite resides, within invaginati...

journal_title：Cell

authors： Gruenberg J,van der Goot FG

更新日期：2006-04-21 00:00:00

abstract：:The high affinity receptor that binds the Fc domain of immunoglobulin G (IgG) subclasses 1 and 3 (Fc gamma RI) mediates important immune defense functions by inducing cell surface changes on human leukocytes. In this article, we document direct high affinity binding of Fc gamma RI to the actin filament cross-linking p...

journal_title：Cell

authors： Ohta Y,Stossel TP,Hartwig JH

更新日期：1991-10-18 00:00:00

abstract：:Hematopoietic stem cells (HSCs) exist in the bone marrow and circulate in the blood. In this issue, Massberg et al. (2007) report that HSCs also travel through the lymphatic system. Furthermore, migration of HSCs--which express Toll-like receptors--allows the recognition of pathogenic molecules in peripheral tissues t...

journal_title：Cell

authors： Welner RS,Kincade PW

更新日期：2007-11-30 00:00:00

abstract：:The recent Zika virus outbreak highlights the need for low-cost diagnostics that can be rapidly developed for distribution and use in pandemic regions. Here, we report a pipeline for the rapid design, assembly, and validation of cell-free, paper-based sensors for the detection of the Zika virus RNA genome. By linking ...

journal_title：Cell

authors： Pardee K,Green AA,Takahashi MK,Braff D,Lambert G,Lee JW,Ferrante T,Ma D,Donghia N,Fan M,Daringer NM,Bosch I,Dudley DM,O'Connor DH,Gehrke L,Collins JJ

更新日期：2016-05-19 00:00:00

abstract：:A cDNA clone encoding a novel hematopoietic growth factor activity produced by a gibbon T cell line has been identified using a mammalian cell expression cloning system. The sequence of this cDNA proved to have significant homology to the sequence encoding murine interleukin 3 (IL-3). The human gene, which was readily...

journal_title：Cell

authors： Yang YC,Ciarletta AB,Temple PA,Chung MP,Kovacic S,Witek-Giannotti JS,Leary AC,Kriz R,Donahue RE,Wong GG

更新日期：1986-10-10 00:00:00

abstract：:The Zika epidemic in the Americas has challenged surveillance and control. As the epidemic appears to be waning, it is unclear whether transmission is still ongoing, which is exacerbated by discrepancies in reporting. To uncover locations with lingering outbreaks, we investigated travel-associated Zika cases to identi...

journal_title：Cell

authors： Grubaugh ND,Saraf S,Gangavarapu K,Watts A,Tan AL,Oidtman RJ,Ladner JT,Oliveira G,Matteson NL,Kraemer MUG,Vogels CBF,Hentoff A,Bhatia D,Stanek D,Scott B,Landis V,Stryker I,Cone MR,Kopp EW 4th,Cannons AC,Heberlein-L

更新日期：2019-08-22 00:00:00

abstract：:Erythroid Krüppel-like factor (EKLF) is necessary for stage-specific expression of the human beta-globin gene. We show that EKLF requires a SWI/SNF-related chromatin remodeling complex, EKLF coactivator-remodeling complex 1 (E-RC1), to generate a DNase I hypersensitive, transcriptionally active beta-globin promoter on...

journal_title：Cell

authors： Armstrong JA,Bieker JJ,Emerson BM

更新日期：1998-10-02 00:00:00